Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002428

Physical Location & Seq

Genomic Coordinates Forward (+)

33126877 .. 33134574

Gene Structure 7698 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1259000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3798 bp

ATGAAATTTGGAGGGGGATTTTTGATTCCCCAGGATGACGATTTACGAGTCACGGATTCTTGGGTACCGGTGACTCCACAGAAGCCGATTCCAGTGAAGCCTCATCCGGTCCCGGTCAACCTTTATGGGAACCTCCGTCAAGGAGGAAATTGGCAACAGCAGTTGACTGGAATTTCAAGGGAGGATGTACCGGTGGGGGCTAGTTACAATGGCATGGCTCAACCTGTTTGCTCAAATGATCAGCTACTTAGAAATGGTTTTGATGAAAGTCCGGGGGAGAACATTCAAATGATCAATCACATTTCGGACTCCTACAATGCAGGATCCTTTACACAGTTGCTCTTTGATGAGGGCTCAAGTTGGAACGATAATCTAATGACACAACAGCTGCTACATGATAATGCTGCGTATGTTGCCTCTGCAAACAGGAACCCGAGCAAAAGTGTGGACATAGCAGCAAACACACCTCTAATTCCTAAGTTGCATCCTCAAATGAGCAACCAAGGAATCGACTCGGGCAGTTTCTTGCTAACCAACCAAAATTGCAACCATGGTTCATATCCTTGGAGTAATGTGGGCATAGCAGTGAACACACATCCAATTCCCAAGTTGCATCCTCAATTAAGAAACCAAGGAATTGACTCGGACAATTTCTTGCTAACCAACCAAAATTGGAACAATGGTTCATATCCTTCAAGTAGTGTGGATGTAGCAACAAAGACAAATCAAATTCCTAAGTTGCATCCTCAATTGAGCAACCAAGGAATCACCCCGGGCAGTTTCTTGCTAACTGACCAAAATTGCAGCAATGGTTCATATCCTTCAAGTAATGTGATGAGCACATCATTGGTTATGGATTTTCCTTCTCAAGTTGACAACAGTTGTAGAGATTCCAACTCAGTTCATTGGTTATCTGCGGATCAAAATCACTGTTCAAGTTCAAATTCAAACCCATTGAGTAATGGTGACAGCTCATCCCAGACATGCCGGTATGGTTTCCCTTCACCACTCCTGTCAAGCTGTGACCTAAACTCCTTACCAAGAATAAAAGCTGACGCTTCCCCATGTGTTGCCAGGCAACACCAGTTCACAACAGACCAGAACAAGAATTTGGAGAACGACCAGCTTTCTGCAATACTGGAATTCTTGAAGGATGAAGATTCTGGCAAGGAAAAAGACATTCAGGTGAAACTAACCATGTCTATAGAAGATGAAGCAATTAAAAAATATAGTGACGAACTCTTGCAGAACATTGTGGAGTCGTCATCTGCAGCCATTTCTACACCGTACAAGGAAAACAAAGATTCTGACAGGGAAGGTGACAGAGGGATTGATCTAAACATTACACCGCAGCAGAAAGCACCAAAGCGAAGAAAACACAGGCCCAAGGTAATCAGAGAAGGCAAGCCCAAAGGGACACCAAAGCCTGCAACTCCAAACAATACAGAGTCTAAGGAGAGCCAACCAGCGAAGAGGAAGTATGTAAGAAAGAGTGTTCAAAAGGAATCCCCAAGTCCACTGGGAGACGGTGCAAGGGAAACAATAGACCCTAATGGTGGAAAAGGTGCAAAATCATGCAAAAGAGCTCTAGATTTTGATTCGGAAAATACCATGGATGAAAACCAATGCAAAGCAGGTGGTCAACAAGAGCAGATGCAGCAAGGGATCAATATGAATTTTGATTCTCAAGGGAAACCTATGGTCCCTGGAACTAGCCAGGGTTTTAAAGCCAGGCCATCAGAGCAAAGTGTCATACATAGCAAATTGAAGGTAGAAAACCAGATTCCAGGAACTATGAATAACTGCACCTCTTCCATGAATCTTATGTCGGATAACTTTGTATTCCTGCCCGAAAGGCGACCATCAGCATCCCTACTTGCTACAACAAAAGACATGCATCTGAAAAAATCGCATGTTATGGGGAGACATGTAGAGAATGGGAGTTCTGATTTATCTCATAGAAGATACATGGATGGATACACTCCCTTTCAGCAACATACCCATGCTAAAGGAGTAGGTCAAGATGCCATTCGAATGAAAACTAGCAGTGAAAGCCTTCAGATGAAAGAAAATATTACCCAGGGTAACTCTCAGTCAGTACAAAAGATCCTCTCCTTCTCTCTTCCAAGAGAAGTAAGGGGATCCAAGAGGGAGTATTGTCGAACGGTTGAGCATACACATCTTTCAACCAATCATCCACCTAGTTCACTATCTTGCCAAGAGATAAAACAATTGGATGGCCATCAGAGAATGCTCAGCCAGGATATCTCACTACGTCATAAGCAGCAGAAATTTGAGAATGGATATTTGAGCATCTATAATATGCCTTGTGAAGTTACACCGGTTGAGGAGTGTCTGGGAATATTTGAAAGAACAGGGCCAAATACAGTCAACTCAAATGGATTCGCATCAAAGAACCATACAATGTTAAGTTCTTCCATTGAACGTAATGAAAGGATGGACAGACAGAACAAAGGCATAGGCAGGTTTATGAGTGACAGTTACACTCAATCAGTTGCTTCTGGGAATAGTTTTCTAAATCCGCAGATCTCATCCACACATTCATGCCAAGATTTCGCTCAAGTTCTTAGCTTTTCCAACCATTCCACAATTGAAGCCTGTAACCAGCTAGCCTCATCATATCCCAGAAAATCCTTTCAACCAGGAAACAAGGAAGTATCTCAACCTCACCACGATAATATTTTAAACATGAAGCAGACTATGGGACCCACACAGGCAGGTATAGATAATGTCCTGCAAGAGAAGGATGCCTTATATGATTATCAGCAGCCTTCTGCAAAAGCAATCGGGTTTACTGTGAGAAGATATGCTACATTACCAGATGACATTGTACATAAATTTAATAATCTAAACCTGAATGGAAGATGCAGCAAAATTTTAGAGCATGAGCAAAATGCACTTGTCCCATACAAAGGAGATGGTGCAGTAGTTCCCTATGAAAGATTTATCAAGAAACGTAAGCCACGGCCCAAAGTAGAGCTTGACCCGGAAACAAATAGGATATGGAATCTCTTGATGGGAATGGAAGGAAGTGGAGGCATTGAAGGAAATGATATGGAAAAGGAGAAGTATTGGGAAGAGGAAAGAAAGGTTTTCCAAGGCCGAGTTGAATCATTCATTGCACGCATGCATCTTGTTCAAGGAGACAGACGTTTTTCAAAATGGAAAGGATCAGTTGTTGACTCAGTGATTGGAGTTTTCCTTACTCAGAATGTTTCAGACCATCTATCAAGCTCTGCCTTTATGTCTTTGGCTGCACGATTTCCTCTTAAGTCAAGCAATCACCAAGCTCAACCCAAGGTTAGGACAAATATATTGGTTAAAGAACCAGCAGTTCACATGACAAGTCCAGATGATGCCACCAAATGGCATGAAGACAGGTCAAGTCAACCAATCTATCACCCGATTTCTATGACACTCCATGAATCTGAAGCAAACCAGAAAGACAGTGAGACCTCAGGGACAGAAAGGAACTTAGTGGATGCACATAGTCAATGTTTGGAGGAAGAATTTGTATCTTCTCAAGATTCTTTTGAATCCTCAGTCACTCAAGGCGCTTTAGGAATCAAATCATGCTCAGGCTCCAACTCAGAAACAGAAGATCCTGGCACTGGGTGCCAACCCAAGGTTCATGTCTCCATTTCAACATGTCAACAGGTGGCGAAGGGGACCAAGTTTCAAGACTTTTACAATCAAGTAAATGGAAGTTCAGTGTCATATGATGGATCCAAGAATGGGCATTTTAAAATATGCACAGCTAAAGAGAAAATCAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003906 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006349 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010468 GO:0016787 GO:0016798 GO:0016799 GO:0019104 GO:0019222 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0034641 GO:0040029 GO:0043076 GO:0043078 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0061458 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Perm-CXXC RRM_DME

Protein Analysis

1266

Amino Acids

140.5

Weight (kDa)

7.0

Isoelectric Point (pI)

46.5

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002428)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04560 AT5G04560 AT5G04560
fragaria_vesca	FvH4_2g10170 FvH4_2g10170 FvH4_2g10170 FvH4_2g10170 FvH4_2g10170
malus_domestica	MD02G1245300.v1.1 MD07G1071800.v1.1 MD09G1259000.v1.1 MD09G1259100.v1.1 MD17G1253500.v1.1 MD17G1253600.v1.1
prunus_persica	Prupe.2G089400_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1
pyrus_communis	pycom02g21110 pycom09g17380 pycom17g25620 pycom17g25630
rosa_chinensis	RchiOBHm_Chr6g0267351 RchiOBHm_Chr6g0267361
rosa_laevigata	RLG00000014013
rosa_multiflora	Rmu_sc0000746.1_g000002 Rmu_sc0019664.1_g000001
rosa_roxburghii	Rroxscaffold_7G00200830
rosa_rugosa	Rorug06G0031600 Rorug06G0031700 Rorug06G0031800 Rorug06G0031900 Rorug06G0032000
rosa_samantha	Rh6AG153500 Rh6BG153200 Rh6CG149300 Rh6DG137600
rosa_wichuraiana	Rw6G013200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1625
Acc36I	ACCTGC	3 cut(s)	1625, 2475, 2729
Acc65I	GGTACC	1 cut(s)	64
AccB1I	GGYRCC	2 cut(s)	64, 3636
AccB7I	CCANNNNNTGG	1 cut(s)	3387
AciI	CCGC	3 cut(s)	917, 1349, 2543
AcoI	YGGCCR	1 cut(s)	2239
AcuI	CTGAAG	2 cut(s)	2042, 3471
AdeI	CACNNNGTG	1 cut(s)	3636
AfaI	GTAC	5 cut(s)	66, 189, 1289, 2100, 2853
AfiI	CCNNNNNNNGG	7 cut(s)	1556, 2933, 3324, 3387, 3632, 3646, 3756
AflII	CTTAAG	1 cut(s)	3290
AflIII	ACRYGT	2 cut(s)	1927, 3668
AgeI	ACCGGT	3 cut(s)	67, 190, 2341
AjuI	GAANNNNNNNTTGG	4 cut(s)	1094, 1126, 3633, 3665
AloI	GAACNNNNNNTCC	2 cut(s)	667, 699
Alw21I	GWGCWC	2 cut(s)	842, 1588
Alw26I	GTCTC	5 cut(s)	1518, 1918, 3159, 3467, 3661
Ama87I	CYCGRG	3 cut(s)	433, 514, 772
AoxI	GGCC	6 cut(s)	1382, 1733, 2239, 2378, 2987, 3121
AsiGI	ACCGGT	3 cut(s)	67, 190, 2341
Asp700I	GAANNNNTTC	1 cut(s)	2055
Asp718I	GGTACC	1 cut(s)	64
AspLEI	GCGC	1 cut(s)	3578
AspS9I	GGNCC	7 cut(s)	109, 1383, 1702, 2378, 2726, 2988, 3690
AsuC2I	CCSGG	5 cut(s)	113, 273, 773, 774, 3008
AsuHPI	GGTGA	9 cut(s)	82, 760, 977, 996, 1198, 1331, 2681, 3296, 3413
AsuII	TTCGAA	1 cut(s)	2032
AsuNHI	GCTAGC	1 cut(s)	2629
AvaI	CYCGRG	3 cut(s)	433, 514, 772
AvaII	GGWCC	4 cut(s)	109, 1702, 2726, 3690
AxyI	CCTNAGG	1 cut(s)	3478
BalI	TGGCCA	1 cut(s)	2241
BamHI	GGATCC	3 cut(s)	323, 2141, 3746
BanI	GGYRCC	2 cut(s)	64, 3636
BanII	GRGCYC	2 cut(s)	356, 1588
BarI	GAAGNNNNNNTAC	2 cut(s)	2815, 2847
BbsI	GAAGAC	1 cut(s)	3402
Bbv12I	GWGCWC	2 cut(s)	842, 1588
BceAI	ACGGC	1 cut(s)	3002
BciVI	GTATCC	1 cut(s)	1970
BclI	TGATCA	2 cut(s)	238, 291
BcnI	CCSGG	5 cut(s)	113, 273, 773, 774, 3008
BcoDI	GTCTC	5 cut(s)	1518, 1918, 3159, 3467, 3661
BfaI	CTAG	6 cut(s)	201, 1589, 1713, 2043, 2202, 2630
BfmI	CTRYAG	2 cut(s)	1203, 1269
BfoI	RGCGCY	1 cut(s)	3579
BfrI	CTTAAG	1 cut(s)	3290
BfuAI	ACCTGC	3 cut(s)	1625, 2475, 2729
BfuI	GTATCC	1 cut(s)	1970
BglI	GCCNNNNNGGC	1 cut(s)	1855
BglII	AGATCT	1 cut(s)	2547
BlpI	GCTNAGC	1 cut(s)	2255
Bme18I	GGWCC	4 cut(s)	109, 1702, 2726, 3690
BmeT110I	CYCGRG	3 cut(s)	433, 514, 772
BmgT120I	GGNCC	7 cut(s)	109, 1383, 1702, 2378, 2726, 2988, 3690
BmrI	ACTGGG	2 cut(s)	1529, 3642
BmtI	GCTAGC	1 cut(s)	2633
BmuI	ACTGGG	2 cut(s)	1529, 3642
BpiI	GAAGAC	1 cut(s)	3402
Bpu10I	CCTNAGC	1 cut(s)	3598
Bpu1102I	GCTNAGC	1 cut(s)	2255
Bpu14I	TTCGAA	1 cut(s)	2032
BpuEI	CTTGAG	6 cut(s)	340, 852, 1671, 2565, 3528, 3555
BpuMI	CCSGG	5 cut(s)	113, 273, 773, 774, 3008
BsaBI	GATNNNNATC	3 cut(s)	924, 2241, 2407
BsaI	GGTCTC	1 cut(s)	3467
BsaWI	WCCGGW	4 cut(s)	67, 106, 190, 2341
BsaXI	ACNNNNNCTCC	2 cut(s)	1016, 1046
Bsc4I	CCNNNNNNNGG	7 cut(s)	1556, 2933, 3324, 3387, 3632, 3646, 3756
Bse118I	RCCGGY	4 cut(s)	67, 190, 987, 2341
Bse1I	ACTGG	6 cut(s)	92, 172, 1084, 1143, 1524, 3637
Bse21I	CCTNAGG	1 cut(s)	3478
Bse3DI	GCAATG	2 cut(s)	814, 3138
Bse8I	GATNNNNATC	3 cut(s)	924, 2241, 2407
BseJI	GATNNNNATC	3 cut(s)	924, 2241, 2407
BseLI	CCNNNNNNNGG	7 cut(s)	1556, 2933, 3324, 3387, 3632, 3646, 3756
BseMI	GCAATG	2 cut(s)	814, 3138
BseMII	CTCAG	9 cut(s)	912, 2105, 2269, 3219, 3242, 3492, 3576, 3612, 3624
BseNI	ACTGG	6 cut(s)	92, 172, 1084, 1143, 1524, 3637
BseRI	GAGGAG	1 cut(s)	2363
BsgI	GTGCAG	3 cut(s)	1789, 2964, 3261
BshFI	GGCC	6 cut(s)	1384, 1735, 2241, 2380, 2989, 3123
BshNI	GGYRCC	2 cut(s)	64, 3636
BshTI	ACCGGT	3 cut(s)	67, 190, 2341
BsiHKAI	GWGCWC	2 cut(s)	842, 1588
BsiHKCI	CYCGRG	3 cut(s)	433, 514, 772
BsiSI	CCGG	9 cut(s)	68, 107, 113, 191, 272, 773, 988, 2342, 3008
BslFI	GGGAC	7 cut(s)	95, 1429, 1688, 2739, 2909, 3496, 3703
BslI	CCNNNNNNNGG	7 cut(s)	1556, 2933, 3324, 3387, 3632, 3646, 3756
BsmAI	GTCTC	5 cut(s)	1518, 1918, 3159, 3467, 3661
BsmBI	CGTCTC	1 cut(s)	1518
BsmFI	GGGAC	7 cut(s)	95, 1429, 1688, 2739, 2909, 3496, 3703
BsmI	GAATGC	1 cut(s)	2256
BsnI	GGCC	6 cut(s)	1384, 1735, 2241, 2380, 2989, 3123
Bso31I	GGTCTC	1 cut(s)	3467
BsoBI	CYCGRG	3 cut(s)	433, 514, 772
Bsp119I	TTCGAA	1 cut(s)	2032
Bsp1286I	GDGCHC	3 cut(s)	356, 842, 1588
Bsp1407I	TGTACA	1 cut(s)	2851
Bsp1720I	GCTNAGC	1 cut(s)	2255
Bsp19I	CCATGG	2 cut(s)	550, 1611
BspACI	CCGC	3 cut(s)	917, 1349, 2543
BspANI	GGCC	6 cut(s)	1384, 1735, 2241, 2380, 2989, 3123
BspCNI	CTCAG	9 cut(s)	911, 2104, 2268, 3218, 3241, 3491, 3575, 3611, 3623
BspMAI	CTGCAG	1 cut(s)	1273
BspMI	ACCTGC	3 cut(s)	1625, 2475, 2729
BspOI	GCTAGC	1 cut(s)	2633
BspT104I	TTCGAA	1 cut(s)	2032
BspT107I	GGYRCC	2 cut(s)	64, 3636
BspTI	CTTAAG	1 cut(s)	3290
BspTNI	GGTCTC	1 cut(s)	3467
BsrDI	GCAATG	2 cut(s)	814, 3138
BsrFI	RCCGGY	4 cut(s)	67, 190, 987, 2341
BsrGI	TGTACA	1 cut(s)	2851
BsrI	ACTGG	6 cut(s)	92, 172, 1084, 1143, 1524, 3637
BssAI	RCCGGY	4 cut(s)	67, 190, 987, 2341
Bst6I	CTCTTC	4 cut(s)	1466, 1816, 2127, 3093
BstAFI	CTTAAG	1 cut(s)	3290
BstAPI	GCANNNNNTGC	1 cut(s)	3636
BstAUI	TGTACA	1 cut(s)	2851
BstBI	TTCGAA	1 cut(s)	2032
BstC8I	GCNNGC	5 cut(s)	1406, 1428, 2631, 3145, 3149
BstDSI	CCRYGG	3 cut(s)	550, 1611, 2984
BstH2I	RGCGCY	1 cut(s)	3579
BstHHI	GCGC	1 cut(s)	3578
BstMAI	GTCTC	5 cut(s)	1518, 1918, 3159, 3467, 3661
BstMWI	GCNNNNNNNGC	6 cut(s)	413, 1657, 1741, 1855, 2052, 3636
BstNSI	RCATGY	6 cut(s)	987, 1897, 1916, 1931, 3151, 3672
BstSFI	CTRYAG	2 cut(s)	1203, 1269
BstV2I	GAAGAC	1 cut(s)	3402
BstX2I	RGATCY	6 cut(s)	323, 2106, 2141, 2547, 3622, 3746
BstYI	RGATCY	6 cut(s)	323, 2106, 2141, 2547, 3622, 3746
Bsu36I	CCTNAGG	1 cut(s)	3478
BsuI	GTATCC	1 cut(s)	1970
BsuRI	GGCC	6 cut(s)	1384, 1735, 2241, 2380, 2989, 3123
BtgI	CCRYGG	3 cut(s)	550, 1611, 2984
BtsI	GCAGTG	2 cut(s)	591, 2053
BtsIMutI	CAGTG	9 cut(s)	99, 591, 928, 1517, 2053, 3213, 3475, 3630, 3738
BveI	ACCTGC	3 cut(s)	1625, 2475, 2729
Cac8I	GCNNGC	5 cut(s)	1406, 1428, 2631, 3145, 3149
CfoI	GCGC	1 cut(s)	3578
Cfr10I	RCCGGY	4 cut(s)	67, 190, 987, 2341
Cfr13I	GGNCC	7 cut(s)	109, 1383, 1702, 2378, 2726, 2988, 3690
Cfr9I	CCCGGG	1 cut(s)	772
CseI	GACGC	1 cut(s)	1064
Csp6I	GTAC	5 cut(s)	65, 188, 1288, 2099, 2852
CspAI	ACCGGT	3 cut(s)	67, 190, 2341
CspCI	CAANNNNNGTGG	4 cut(s)	684, 719, 1618, 1653
CviQI	GTAC	5 cut(s)	65, 188, 1288, 2099, 2852
DraI	TTTAAA	3 cut(s)	1726, 2706, 3766
DraIII	CACNNNGTG	1 cut(s)	3636
EaeI	YGGCCR	1 cut(s)	2239
Eam1104I	CTCTTC	4 cut(s)	1466, 1816, 2127, 3093
EarI	CTCTTC	4 cut(s)	1466, 1816, 2127, 3093
Ecl136II	GAGCTC	1 cut(s)	1586
Eco24I	GRGCYC	2 cut(s)	356, 1588
Eco31I	GGTCTC	1 cut(s)	3467
Eco32I	GATATC	1 cut(s)	2266
Eco47I	GGWCC	4 cut(s)	109, 1702, 2726, 3690
Eco53kI	GAGCTC	1 cut(s)	1586
Eco57I	CTGAAG	2 cut(s)	2042, 3471
Eco81I	CCTNAGG	1 cut(s)	3478
Eco88I	CYCGRG	3 cut(s)	433, 514, 772
EcoICRI	GAGCTC	1 cut(s)	1586
EcoO109I	RGGNCCY	1 cut(s)	2726
EcoRI	GAATTC	1 cut(s)	1142
EcoRV	GATATC	1 cut(s)	2266
EcoT22I	ATGCAT	2 cut(s)	1899, 3153
EcoT38I	GRGCYC	2 cut(s)	356, 1588
Esp3I	CGTCTC	1 cut(s)	1518
FalI	AAGNNNNNCTT	4 cut(s)	2756, 2788, 2985, 3017
FaqI	GGGAC	7 cut(s)	95, 1429, 1688, 2739, 2909, 3496, 3703
FauNDI	CATATG	1 cut(s)	3739
FbaI	TGATCA	2 cut(s)	238, 291
FriOI	GRGCYC	2 cut(s)	356, 1588
FspBI	CTAG	6 cut(s)	201, 1589, 1713, 2043, 2202, 2630
GlaI	GCGC	1 cut(s)	3577
HaeII	RGCGCY	1 cut(s)	3579
HaeIII	GGCC	6 cut(s)	1384, 1735, 2241, 2380, 2989, 3123
HapII	CCGG	9 cut(s)	68, 107, 113, 191, 272, 773, 988, 2342, 3008
HgaI	GACGC	1 cut(s)	1064
HhaI	GCGC	1 cut(s)	3578
Hin6I	GCGC	1 cut(s)	3576
HinP1I	GCGC	1 cut(s)	3576
HincII	GTYRAC	8 cut(s)	118, 165, 874, 1643, 2392, 3202, 3410, 3674
HindII	GTYRAC	8 cut(s)	118, 165, 874, 1643, 2392, 3202, 3410, 3674
HpaII	CCGG	9 cut(s)	68, 107, 113, 191, 272, 773, 988, 2342, 3008
HphI	GGTGA	9 cut(s)	82, 760, 977, 996, 1198, 1331, 2681, 3296, 3413
Hpy188III	TCNNGA	8 cut(s)	1147, 1589, 2021, 2971, 3034, 3371, 3545, 3701
HpyCH4IV	ACGT	4 cut(s)	2275, 2446, 2977, 3172
HpyF10VI	GCNNNNNNNGC	6 cut(s)	413, 1657, 1741, 1855, 2052, 3636
HpySE526I	ACGT	4 cut(s)	2275, 2446, 2977, 3172
HspAI	GCGC	1 cut(s)	3576
KflI	GGGWCCC	1 cut(s)	2726
KpnI	GGTACC	1 cut(s)	68
Ksp22I	TGATCA	2 cut(s)	238, 291
LmnI	GCTCC	1 cut(s)	3608
MaeI	CTAG	6 cut(s)	201, 1589, 1713, 2043, 2202, 2630
MaeII	ACGT	4 cut(s)	2275, 2446, 2977, 3172
MfeI	CAATTG	3 cut(s)	749, 2231, 2610
MflI	RGATCY	6 cut(s)	323, 2106, 2141, 2547, 3622, 3746
MhlI	GDGCHC	3 cut(s)	356, 842, 1588
MlsI	TGGCCA	1 cut(s)	2241
MluNI	TGGCCA	1 cut(s)	2241
MlyI	GAGTC	8 cut(s)	57, 67, 302, 506, 635, 1268, 1457, 3197
MmeI	TCCRAC	5 cut(s)	341, 918, 1809, 2622, 3630
Mox20I	TGGCCA	1 cut(s)	2241
Mph1103I	ATGCAT	2 cut(s)	1899, 3153
MroXI	GAANNNNTTC	1 cut(s)	2055
MscI	TGGCCA	1 cut(s)	2241
MseI	TTAA	9 cut(s)	623, 1221, 1725, 2429, 2705, 2862, 3291, 3342, 3765
MslI	CAYNNNNRTG	7 cut(s)	287, 399, 584, 1184, 2001, 2033, 2563
Msp20I	TGGCCA	1 cut(s)	2241
MspA1I	CMGCKG	1 cut(s)	388
MspCI	CTTAAG	1 cut(s)	3290
MspI	CCGG	9 cut(s)	68, 107, 113, 191, 272, 773, 988, 2342, 3008
MunI	CAATTG	3 cut(s)	749, 2231, 2610
Mva1269I	GAATGC	1 cut(s)	2256
MwoI	GCNNNNNNNGC	6 cut(s)	413, 1657, 1741, 1855, 2052, 3636
NciI	CCSGG	5 cut(s)	113, 273, 773, 774, 3008
NcoI	CCATGG	2 cut(s)	550, 1611
NdeI	CATATG	1 cut(s)	3739
NheI	GCTAGC	1 cut(s)	2629
NmeAIII	GCCGAG	1 cut(s)	3149
NmuCI	GTSAC	8 cut(s)	49, 70, 965, 1022, 1232, 1319, 2495, 3565
NsiI	ATGCAT	2 cut(s)	1899, 3153
NspI	RCATGY	6 cut(s)	987, 1897, 1916, 1931, 3151, 3672
NspV	TTCGAA	1 cut(s)	2032
PaeI	GCATGC	1 cut(s)	3151
PaqCI	CACCTGC	1 cut(s)	1625
PasI	CCCWGGG	1 cut(s)	2080
PciI	ACATGT	2 cut(s)	1927, 3668
PctI	GAATGC	1 cut(s)	2256
PdmI	GAANNNNTTC	1 cut(s)	2055
PflMI	CCANNNNNTGG	1 cut(s)	3387
PfoI	TCCNGGA	1 cut(s)	1786
PinAI	ACCGGT	3 cut(s)	67, 190, 2341
PleI	GAGTC	8 cut(s)	56, 67, 302, 506, 635, 1267, 1456, 3197
PpsI	GAGTC	8 cut(s)	56, 67, 302, 506, 635, 1267, 1456, 3197
PpuMI	RGGWCCY	1 cut(s)	2726
PscI	ACATGT	2 cut(s)	1927, 3668
Psp124BI	GAGCTC	1 cut(s)	1588
Psp5II	RGGWCCY	1 cut(s)	2726
PspPI	GGNCC	7 cut(s)	109, 1383, 1702, 2378, 2726, 2988, 3690
PspPPI	RGGWCCY	1 cut(s)	2726
PstI	CTGCAG	1 cut(s)	1273
PsuI	RGATCY	6 cut(s)	323, 2106, 2141, 2547, 3622, 3746
PvuII	CAGCTG	1 cut(s)	388
RsaI	GTAC	5 cut(s)	66, 189, 1289, 2100, 2853
RsaNI	GTAC	5 cut(s)	65, 188, 1288, 2099, 2852
RseI	CAYNNNNRTG	7 cut(s)	287, 399, 584, 1184, 2001, 2033, 2563
SacI	GAGCTC	1 cut(s)	1588
SaqAI	TTAA	9 cut(s)	623, 1221, 1725, 2429, 2705, 2862, 3291, 3342, 3765
Sau96I	GGNCC	7 cut(s)	109, 1383, 1702, 2378, 2726, 2988, 3690
SchI	GAGTC	8 cut(s)	57, 67, 302, 506, 635, 1268, 1457, 3197
SduI	GDGCHC	3 cut(s)	356, 842, 1588
SfcI	CTRYAG	2 cut(s)	1203, 1269
SfuI	TTCGAA	1 cut(s)	2032
SinI	GGWCC	4 cut(s)	109, 1702, 2726, 3690
SmaI	CCCGGG	1 cut(s)	774
SmiMI	CAYNNNNRTG	7 cut(s)	287, 399, 584, 1184, 2001, 2033, 2563
SmlI	CTYRAG	7 cut(s)	355, 867, 1686, 2580, 3290, 3543, 3570
SmoI	CTYRAG	7 cut(s)	355, 867, 1686, 2580, 3290, 3543, 3570
SphI	GCATGC	1 cut(s)	3151
SsiI	CCGC	3 cut(s)	917, 1349, 2543
SspI	AATATT	3 cut(s)	2074, 2364, 2701
SspMI	CTAG	6 cut(s)	201, 1589, 1713, 2043, 2202, 2630
SstI	GAGCTC	1 cut(s)	1588
TaiI	ACGT	4 cut(s)	2278, 2449, 2980, 3175
TaqI	TCGA	3 cut(s)	510, 2032, 2161
TatI	WGTACW	2 cut(s)	2098, 2851
Tru1I	TTAA	9 cut(s)	623, 1221, 1725, 2429, 2705, 2862, 3291, 3342, 3765
Tru9I	TTAA	9 cut(s)	623, 1221, 1725, 2429, 2705, 2862, 3291, 3342, 3765
TscAI	CASTG	9 cut(s)	99, 591, 935, 1524, 2053, 3213, 3475, 3637, 3738
TseFI	GTSAC	8 cut(s)	49, 70, 965, 1022, 1232, 1319, 2495, 3565
Tsp45I	GTSAC	8 cut(s)	49, 70, 965, 1022, 1232, 1319, 2495, 3565
TspGWI	ACGGA	2 cut(s)	68, 125
TspMI	CCCGGG	1 cut(s)	772
TspRI	CASTG	9 cut(s)	99, 591, 935, 1524, 2053, 3213, 3475, 3637, 3738
Van91I	CCANNNNNTGG	1 cut(s)	3387
Vha464I	CTTAAG	1 cut(s)	3290
VpaK11BI	GGWCC	4 cut(s)	109, 1702, 2726, 3690
XbaI	TCTAGA	1 cut(s)	1588
XceI	RCATGY	6 cut(s)	987, 1897, 1916, 1931, 3151, 3672
XmaI	CCCGGG	1 cut(s)	772
XmnI	GAANNNNTTC	1 cut(s)	2055
XspI	CTAG	6 cut(s)	201, 1589, 1713, 2043, 2202, 2630
Zsp2I	ATGCAT	2 cut(s)	1899, 3153

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.