MD02G1276400.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008120
Physical Location & Seq
Forward (+)
33150169 .. 33152062
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1276400.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGGCGAACCATTCGTCGCATGCCCACCACCATGTAACGTAATCCAGATCCCGAATCCAGAATTCATCCAGAGCCAGGATCAGGAGCAGATCCCGCCTCCACCGAATGAAGACGAGCTGGCGATTCCGGGCAGACTCCATTGGCACTGGTTCGACGAAGAAGAAGAGCATCTAATGGGTGGCGAAGATCTGCCCGGGGTGTCTTACGTCCTCGAGGATAGCTATGAAGCCCAAATGGCTGCTAGAGAGAAGACGAGTAAGATAATGGCGCAAAGATTGGCACGAACATCTCCCGAAGATAAAGAAAACTGGGATTATTTGGTCAGTCGAGTCAGTTGGAAGCCAAAACATGGACAATGGCTTTGTCTCGTCAAAGAGCTGCCCAATTGTTTGTATTGTAGAATTTGTTGTACTGTTGGCCACAAGACTCAGGACTGCCCAGATAATGATGGCTCTCATGATCCTGCCTTTGTTTACATGATATGTGGTATTTGTTCAAGACGGGGTCACTGGGGTAACACCTGCCCATACCAGTACTATGTCCCAAGGAACGCAGCAGTTGGCCCTGGCGGTAGACTAGTCTGCAGGTGTTGTGAGAAAGAGGGTGGACACTCGGACCCTGATGACGATGAATGGATCGGAGTTGCTTTTGTGAATGATTGTGGGACTTGTGGTGCTGTTGGCAAGCACCGGTCTGAAGACTGCCCCAAGAAAGAGGGAACAGGGAAAATCGACCGGAAGACTGCCCTGAGAAAGAAGAAACAAACTTCCGTCCCAAGAAAGAAGAAACATGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

266

Amino Acids

29.87

Weight (kDa)

6.07

Isoelectric Point (pI)

45.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 530, 576
AbsI CCTCGAGG 1 cut(s) 212
Acc36I ACCTGC 2 cut(s) 530, 576
AccB7I CCANNNNNTGG 1 cut(s) 350
AccI GTMKAC 1 cut(s) 574
AciI CCGC 2 cut(s) 95, 570
AclWI GGATC 5 cut(s) 43, 85, 87, 455, 644
AcoI YGGCCR 1 cut(s) 418
AcsI RAATTY 2 cut(s) 62, 402
AcuI CTGAAG 1 cut(s) 717
AfaI GTAC 2 cut(s) 412, 536
AfiI CCNNNNNNNGG 3 cut(s) 76, 82, 350
AgeI ACCGGT 1 cut(s) 690
AgsI TTSAA 1 cut(s) 498
AhlI ACTAGT 1 cut(s) 577
AjnI CCWGG 2 cut(s) 75, 565
AluBI AGCT 3 cut(s) 118, 222, 379
AluI AGCT 3 cut(s) 118, 222, 379
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 5 cut(s) 43, 85, 87, 455, 644
Ama87I CYCGRG 2 cut(s) 194, 212
AoxI GGCC 2 cut(s) 418, 562
ApeKI GCWGC 3 cut(s) 239, 379, 554
ApoI RAATTY 2 cut(s) 62, 402
ArsI GACNNNNNNTTYG 3 cut(s) 32, 345, 377
AsiGI ACCGGT 1 cut(s) 690
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 2 cut(s) 563, 616
AsuC2I CCSGG 3 cut(s) 129, 195, 196
AvaI CYCGRG 2 cut(s) 194, 212
AvaII GGWCC 1 cut(s) 616
BalI TGGCCA 1 cut(s) 420
BbsI GAAGAC 4 cut(s) 117, 257, 705, 746
BbvI GCAGC 3 cut(s) 226, 366, 566
BccI CCATC 1 cut(s) 443
BciT130I CCWGG 2 cut(s) 77, 567
BcnI CCSGG 3 cut(s) 129, 195, 196
BcoDI GTCTC 1 cut(s) 371
BcuI ACTAGT 1 cut(s) 577
BfaI CTAG 2 cut(s) 243, 578
BfmI CTRYAG 1 cut(s) 583
BfuAI ACCTGC 2 cut(s) 530, 576
BglI GCCNNNNNGGC 1 cut(s) 236
BglII AGATCT 1 cut(s) 187
BisI GCNGC 3 cut(s) 240, 380, 555
BlsI GCNGC 3 cut(s) 241, 381, 556
BmcAI AGTACT 1 cut(s) 536
Bme1390I CCNGG 5 cut(s) 77, 129, 195, 196, 567
Bme18I GGWCC 1 cut(s) 616
BmeT110I CYCGRG 2 cut(s) 194, 212
BmgT120I GGNCC 2 cut(s) 563, 616
BmiI GGNNCC 1 cut(s) 618
BmrFI CCNGG 5 cut(s) 77, 129, 195, 196, 567
BmrI ACTGGG 2 cut(s) 319, 520
BmsI GCATC 1 cut(s) 178
BmuI ACTGGG 2 cut(s) 319, 520
BpiI GAAGAC 4 cut(s) 117, 257, 705, 746
BpuMI CCSGG 3 cut(s) 129, 195, 196
BsaJI CCNNGG 4 cut(s) 194, 195, 545, 565
BsaWI WCCGGW 2 cut(s) 690, 735
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 82, 350
Bse118I RCCGGY 1 cut(s) 690
Bse1I ACTGG 4 cut(s) 152, 314, 515, 532
BseBI CCWGG 2 cut(s) 77, 567
BseDI CCNNGG 4 cut(s) 194, 195, 545, 565
BseGI GGATG 1 cut(s) 66
BseLI CCNNNNNNNGG 3 cut(s) 76, 82, 350
BseMII CTCAG 2 cut(s) 443, 740
BseNI ACTGG 4 cut(s) 152, 314, 515, 532
BseXI GCAGC 3 cut(s) 226, 366, 566
Bsh1285I CGRYCG 1 cut(s) 736
BshFI GGCC 2 cut(s) 420, 564
BshTI ACCGGT 1 cut(s) 690
BsiEI CGRYCG 1 cut(s) 736
BsiHKCI CYCGRG 2 cut(s) 194, 212
BsiSI CCGG 4 cut(s) 128, 195, 691, 736
BslFI GGGAC 3 cut(s) 527, 679, 758
BslI CCNNNNNNNGG 3 cut(s) 76, 82, 350
BsmAI GTCTC 1 cut(s) 371
BsmFI GGGAC 3 cut(s) 527, 679, 758
BsnI GGCC 2 cut(s) 420, 564
BsoBI CYCGRG 2 cut(s) 194, 212
Bsp143I GATC 6 cut(s) 48, 79, 90, 187, 460, 636
BspACI CCGC 2 cut(s) 95, 570
BspANI GGCC 2 cut(s) 420, 564
BspCNI CTCAG 2 cut(s) 442, 741
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 1 cut(s) 618
BspMAI CTGCAG 1 cut(s) 587
BspMI ACCTGC 2 cut(s) 530, 576
BspPI GGATC 5 cut(s) 43, 85, 87, 455, 644
BspQI GCTCTTC 1 cut(s) 159
BsrFI RCCGGY 1 cut(s) 690
BsrI ACTGG 4 cut(s) 152, 314, 515, 532
BssAI RCCGGY 1 cut(s) 690
BssECI CCNNGG 4 cut(s) 194, 195, 545, 565
BssMI GATC 6 cut(s) 48, 79, 90, 187, 460, 636
BssT1I CCWWGG 1 cut(s) 545
Bst2UI CCWGG 2 cut(s) 77, 567
Bst4CI ACNGT 1 cut(s) 415
Bst6I CTCTTC 1 cut(s) 159
BstC8I GCNNGC 3 cut(s) 22, 120, 686
BstDEI CTNAG 2 cut(s) 429, 749
BstF5I GGATG 1 cut(s) 66
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 6 cut(s) 51, 82, 93, 190, 463, 639
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 6 cut(s) 48, 79, 90, 187, 460, 636
BstMCI CGRYCG 1 cut(s) 736
BstMWI GCNNNNNNNGC 2 cut(s) 94, 236
BstNI CCWGG 2 cut(s) 77, 567
BstNSI RCATGY 1 cut(s) 24
BstSCI CCNGG 5 cut(s) 75, 127, 193, 194, 565
BstSFI CTRYAG 1 cut(s) 583
BstV1I GCAGC 3 cut(s) 226, 366, 566
BstV2I GAAGAC 4 cut(s) 117, 257, 705, 746
BstX2I RGATCY 3 cut(s) 48, 90, 187
BstYI RGATCY 3 cut(s) 48, 90, 187
BsuRI GGCC 2 cut(s) 420, 564
BtsCI GGATG 1 cut(s) 66
BtsIMutI CAGTG 2 cut(s) 145, 508
BveI ACCTGC 2 cut(s) 530, 576
Cac8I GCNNGC 3 cut(s) 22, 120, 686
CciI TCATGA 1 cut(s) 457
CfoI GCGC 1 cut(s) 271
Cfr10I RCCGGY 1 cut(s) 690
Cfr13I GGNCC 2 cut(s) 563, 616
Cfr9I CCCGGG 1 cut(s) 194
Csp6I GTAC 2 cut(s) 411, 535
CspAI ACCGGT 1 cut(s) 690
CviAII CATG 6 cut(s) 21, 33, 350, 458, 478, 791
CviQI GTAC 2 cut(s) 411, 535
DdeI CTNAG 2 cut(s) 429, 749
DpnI GATC 6 cut(s) 50, 81, 92, 189, 462, 638
DpnII GATC 6 cut(s) 48, 79, 90, 187, 460, 636
EaeI YGGCCR 1 cut(s) 418
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 545
Eco47I GGWCC 1 cut(s) 616
Eco57I CTGAAG 1 cut(s) 717
Eco88I CYCGRG 2 cut(s) 194, 212
EcoRI GAATTC 1 cut(s) 62
EcoRII CCWGG 2 cut(s) 75, 565
EcoT14I CCWWGG 1 cut(s) 545
ErhI CCWWGG 1 cut(s) 545
FaeI CATG 6 cut(s) 24, 36, 353, 461, 481, 794
FaqI GGGAC 3 cut(s) 527, 679, 758
FatI CATG 6 cut(s) 20, 32, 349, 457, 477, 790
FauI CCCGC 1 cut(s) 102
FblI GTMKAC 1 cut(s) 574
Fnu4HI GCNGC 3 cut(s) 240, 380, 555
FokI GGATG 1 cut(s) 53
Fsp4HI GCNGC 3 cut(s) 240, 380, 555
FspBI CTAG 2 cut(s) 243, 578
GlaI GCGC 1 cut(s) 270
GluI GCNGC 3 cut(s) 240, 380, 555
HaeIII GGCC 2 cut(s) 420, 564
HapII CCGG 4 cut(s) 128, 195, 691, 736
HhaI GCGC 1 cut(s) 271
Hin1II CATG 6 cut(s) 24, 36, 353, 461, 481, 794
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HinfI GANTC 5 cut(s) 55, 124, 135, 330, 427
HpaII CCGG 4 cut(s) 128, 195, 691, 736
Hpy166II GTNNAC 3 cut(s) 475, 575, 608
Hpy188I TCNGA 3 cut(s) 616, 641, 697
Hpy188III TCNNGA 9 cut(s) 46, 52, 59, 70, 83, 293, 431, 458, 498
Hpy8I GTNNAC 3 cut(s) 475, 575, 608
Hpy99I CGWCG 2 cut(s) 20, 158
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4IV ACGT 2 cut(s) 39, 207
HpyCH4V TGCA 1 cut(s) 585
HpyF10VI GCNNNNNNNGC 2 cut(s) 94, 236
HpyF3I CTNAG 2 cut(s) 429, 749
HpySE526I ACGT 2 cut(s) 39, 207
Hsp92II CATG 6 cut(s) 24, 36, 353, 461, 481, 794
HspAI GCGC 1 cut(s) 269
Kzo9I GATC 6 cut(s) 48, 79, 90, 187, 460, 636
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 1 cut(s) 85
Lsp1109I GCAGC 3 cut(s) 226, 366, 566
LweI GCATC 1 cut(s) 178
MaeI CTAG 2 cut(s) 243, 578
MaeII ACGT 2 cut(s) 39, 207
MaeIII GTNAC 3 cut(s) 35, 506, 515
MalI GATC 6 cut(s) 50, 81, 92, 189, 462, 638
MboI GATC 6 cut(s) 48, 79, 90, 187, 460, 636
MfeI CAATTG 1 cut(s) 385
MflI RGATCY 3 cut(s) 48, 90, 187
MlsI TGGCCA 1 cut(s) 420
MluCI AATT 3 cut(s) 62, 385, 402
MluNI TGGCCA 1 cut(s) 420
MlyI GAGTC 3 cut(s) 129, 339, 421
MmeI TCCRAC 1 cut(s) 317
MnlI CCTC 5 cut(s) 108, 208, 221, 595, 709
Mox20I TGGCCA 1 cut(s) 420
MscI TGGCCA 1 cut(s) 420
MslI CAYNNNNRTG 1 cut(s) 31
Msp20I TGGCCA 1 cut(s) 420
MspI CCGG 4 cut(s) 128, 195, 691, 736
MspR9I CCNGG 5 cut(s) 77, 129, 195, 196, 567
MunI CAATTG 1 cut(s) 385
MvaI CCWGG 2 cut(s) 77, 567
MwoI GCNNNNNNNGC 2 cut(s) 94, 236
NciI CCSGG 3 cut(s) 129, 195, 196
NdeII GATC 6 cut(s) 48, 79, 90, 187, 460, 636
NlaIII CATG 6 cut(s) 24, 36, 353, 461, 481, 794
NlaIV GGNNCC 1 cut(s) 618
NmuCI GTSAC 1 cut(s) 506
NspI RCATGY 1 cut(s) 24
PaeI GCATGC 1 cut(s) 24
PaeR7I CTCGAG 1 cut(s) 212
PagI TCATGA 1 cut(s) 457
PaqCI CACCTGC 2 cut(s) 530, 576
PciSI GCTCTTC 1 cut(s) 159
PfeI GAWTC 2 cut(s) 55, 124
PflFI GACNNNGTC 1 cut(s) 504
PflMI CCANNNNNTGG 1 cut(s) 350
PinAI ACCGGT 1 cut(s) 690
PkrI GCNGC 3 cut(s) 241, 381, 556
PleI GAGTC 3 cut(s) 129, 338, 421
PpsI GAGTC 3 cut(s) 129, 338, 421
Psp6I CCWGG 2 cut(s) 75, 565
PspGI CCWGG 2 cut(s) 75, 565
PspN4I GGNNCC 1 cut(s) 618
PspPI GGNCC 2 cut(s) 563, 616
PspXI VCTCGAGB 1 cut(s) 212
PstI CTGCAG 1 cut(s) 587
PsuI RGATCY 3 cut(s) 48, 90, 187
PsyI GACNNNGTC 1 cut(s) 504
RsaI GTAC 2 cut(s) 412, 536
RsaNI GTAC 2 cut(s) 411, 535
RseI CAYNNNNRTG 1 cut(s) 31
SapI GCTCTTC 1 cut(s) 159
SatI GCNGC 3 cut(s) 240, 380, 555
Sau3AI GATC 6 cut(s) 48, 79, 90, 187, 460, 636
Sau96I GGNCC 2 cut(s) 563, 616
ScaI AGTACT 1 cut(s) 536
SchI GAGTC 3 cut(s) 129, 339, 421
ScrFI CCNGG 5 cut(s) 77, 129, 195, 196, 567
SetI ASST 7 cut(s) 42, 120, 210, 224, 381, 524, 590
SfaNI GCATC 1 cut(s) 178
SfcI CTRYAG 1 cut(s) 583
Sfr274I CTCGAG 1 cut(s) 212
SinI GGWCC 1 cut(s) 616
SlaI CTCGAG 1 cut(s) 212
SmaI CCCGGG 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 31
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
SpeI ACTAGT 1 cut(s) 577
SphI GCATGC 1 cut(s) 24
Sse9I AATT 3 cut(s) 62, 385, 402
SsiI CCGC 2 cut(s) 95, 570
SspMI CTAG 2 cut(s) 243, 578
StyD4I CCNGG 5 cut(s) 75, 127, 193, 194, 565
StyI CCWWGG 1 cut(s) 545
TaaI ACNGT 1 cut(s) 415
TaiI ACGT 2 cut(s) 42, 210
TaqI TCGA 4 cut(s) 153, 213, 328, 732
TasI AATT 3 cut(s) 62, 385, 402
TatI WGTACW 2 cut(s) 410, 534
TfiI GAWTC 2 cut(s) 55, 124
TscAI CASTG 2 cut(s) 152, 515
TseFI GTSAC 1 cut(s) 506
TseI GCWGC 3 cut(s) 239, 379, 554
Tsp45I GTSAC 1 cut(s) 506
TspDTI ATGAA 4 cut(s) 55, 123, 240, 645
TspGWI ACGGA 1 cut(s) 760
TspMI CCCGGG 1 cut(s) 194
TspRI CASTG 2 cut(s) 152, 515
Tth111I GACNNNGTC 1 cut(s) 504
Van91I CCANNNNNTGG 1 cut(s) 350
VpaK11BI GGWCC 1 cut(s) 616
XapI RAATTY 2 cut(s) 62, 402
XceI RCATGY 1 cut(s) 24
XhoI CTCGAG 1 cut(s) 212
XmaI CCCGGG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 574
XspI CTAG 2 cut(s) 243, 578
ZrmI AGTACT 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.