Rw5G033450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008120
Physical Location & Seq
Forward (+)
56928197 .. 56931598
3402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G033450.1

Sequence Viewer

Length: 648 bp
ATGCCCACGCAGATTAGCTACAGCAGGGGGGCACGAGAAGTCAAGGTGGAAGAAGTCATTTTGAAATTAAGATCCGACCTGAGCCAGAAGTGTTCGAATTCCCTGCAAATCTCTAGGCCCCGGGAGATCAATATTCAAGGAGGAGTACTGACGGTGGAAGACTGGCAGGAGAAATTCAGATACGTGAAATCAGGTTACGGTCATGGTCACGGTCAGGGCGAGAATCCAACCCAGCAGATCGATAAGGACCCGGAGATCAATATTGAAGCAGGTGGCAGTGGTAAAAGTACAGATAATCCTCCTGCAAGGAGTTGGGGAGATGAATCAGATGATGATATGCTTCTTCACATATATGGAGTACCCTTTGCTGATGACGAGTTTGACGGACCTCTTGCAAGATGTGAGCTTTGTGGTCAGACCGTATCTCACTATGCTGCAAGCTGCCCCTACTTGATTCATTTGCCACACCACATAACTCTTCCTCGTGGTTATGACAGAGCTTGTAAGTGTTGTGGTAGAAAGGATGGCCACCCTGGTCAGAAGTGGGAGGGATTTGCTATCCGCAAGTATTGTTTTAATTGTTGTCGGGGAGCTGACCACTGGGATGGTGACCCAGAATGTCCCAAGAAAAAGCAACGTGTTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

215

Amino Acids

24.12

Weight (kDa)

6.3

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 260
Acc36I ACCTGC 1 cut(s) 260
AciI CCGC 1 cut(s) 562
AclWI GGATC 1 cut(s) 66
AcoI YGGCCR 1 cut(s) 526
AcsI RAATTY 2 cut(s) 97, 173
AfaI GTAC 3 cut(s) 147, 289, 360
AflIII ACRYGT 1 cut(s) 637
AgsI TTSAA 3 cut(s) 64, 137, 266
AjnI CCWGG 1 cut(s) 532
AluBI AGCT 5 cut(s) 18, 406, 441, 500, 593
AluI AGCT 5 cut(s) 18, 406, 441, 500, 593
AlwI GGATC 1 cut(s) 66
Ama87I CYCGRG 1 cut(s) 120
AoxI GGCC 2 cut(s) 116, 526
ApeKI GCWGC 2 cut(s) 434, 441
ApoI RAATTY 2 cut(s) 97, 173
AspS9I GGNCC 3 cut(s) 117, 247, 386
AsuC2I CCSGG 3 cut(s) 121, 122, 251
AsuHPI GGTGA 1 cut(s) 620
AsuII TTCGAA 1 cut(s) 95
AvaI CYCGRG 1 cut(s) 120
AvaII GGWCC 2 cut(s) 247, 386
BaeGI GKGCMC 1 cut(s) 34
BalI TGGCCA 1 cut(s) 528
BauI CACGAG 2 cut(s) 33, 483
BbsI GAAGAC 1 cut(s) 165
BbvI GCAGC 2 cut(s) 421, 428
BccI CCATC 2 cut(s) 518, 599
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 1 cut(s) 534
BcnI CCSGG 3 cut(s) 121, 122, 251
BfaI CTAG 1 cut(s) 114
BfmI CTRYAG 1 cut(s) 19
BfuAI ACCTGC 1 cut(s) 260
BisI GCNGC 2 cut(s) 435, 442
BlsI GCNGC 2 cut(s) 436, 443
BmcAI AGTACT 1 cut(s) 147
Bme1390I CCNGG 4 cut(s) 121, 122, 251, 534
Bme18I GGWCC 2 cut(s) 247, 386
BmeT110I CYCGRG 1 cut(s) 120
BmgT120I GGNCC 3 cut(s) 117, 247, 386
BmiI GGNNCC 2 cut(s) 119, 249
BmrFI CCNGG 4 cut(s) 121, 122, 251, 534
BmrI ACTGGG 1 cut(s) 610
BmuI ACTGGG 1 cut(s) 610
BpiI GAAGAC 1 cut(s) 165
Bpu10I CCTNAGC 1 cut(s) 80
Bpu14I TTCGAA 1 cut(s) 95
BpuMI CCSGG 3 cut(s) 121, 122, 251
Bsa29I ATCGAT 1 cut(s) 240
BsaAI YACGTR 1 cut(s) 184
BsaJI CCNNGG 3 cut(s) 119, 120, 532
Bse1I ACTGG 2 cut(s) 167, 605
BseBI CCWGG 1 cut(s) 534
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 3 cut(s) 119, 120, 532
BseGI GGATG 2 cut(s) 529, 610
BseMII CTCAG 1 cut(s) 71
BseNI ACTGG 2 cut(s) 167, 605
BseRI GAGGAG 1 cut(s) 156
BseSI GKGCMC 1 cut(s) 34
BseXI GCAGC 2 cut(s) 421, 428
BseYI CCCAGC 1 cut(s) 231
BshFI GGCC 2 cut(s) 118, 528
BshVI ATCGAT 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 120
BsiSI CCGG 2 cut(s) 121, 251
BslFI GGGAC 1 cut(s) 606
BsmFI GGGAC 1 cut(s) 606
BsnI GGCC 2 cut(s) 118, 528
BsoBI CYCGRG 1 cut(s) 120
Bsp119I TTCGAA 1 cut(s) 95
Bsp1286I GDGCHC 1 cut(s) 34
Bsp143I GATC 4 cut(s) 71, 126, 237, 255
BspACI CCGC 1 cut(s) 562
BspANI GGCC 2 cut(s) 118, 528
BspCNI CTCAG 1 cut(s) 72
BspDI ATCGAT 1 cut(s) 240
BspLI GGNNCC 2 cut(s) 119, 249
BspMI ACCTGC 1 cut(s) 260
BspPI GGATC 1 cut(s) 66
BspT104I TTCGAA 1 cut(s) 95
BsrI ACTGG 2 cut(s) 167, 605
BssECI CCNNGG 3 cut(s) 119, 120, 532
BssMI GATC 4 cut(s) 71, 126, 237, 255
BssSI CACGAG 2 cut(s) 33, 483
Bst2BI CACGAG 2 cut(s) 33, 483
Bst2UI CCWGG 1 cut(s) 534
Bst4CI ACNGT 4 cut(s) 154, 200, 212, 421
Bst6I CTCTTC 1 cut(s) 483
BstBAI YACGTR 1 cut(s) 184
BstBI TTCGAA 1 cut(s) 95
BstC8I GCNNGC 1 cut(s) 439
BstDEI CTNAG 1 cut(s) 80
BstEII GGTNACC 1 cut(s) 608
BstF5I GGATG 2 cut(s) 529, 610
BstKTI GATC 4 cut(s) 74, 129, 240, 258
BstMBI GATC 4 cut(s) 71, 126, 237, 255
BstNI CCWGG 1 cut(s) 534
BstPI GGTNACC 1 cut(s) 608
BstSCI CCNGG 4 cut(s) 119, 120, 249, 532
BstSFI CTRYAG 1 cut(s) 19
BstSLI GKGCMC 1 cut(s) 34
BstV1I GCAGC 2 cut(s) 421, 428
BstV2I GAAGAC 1 cut(s) 165
BstX2I RGATCY 1 cut(s) 71
BstXI CCANNNNNNTGG 1 cut(s) 605
BstYI RGATCY 1 cut(s) 71
Bsu15I ATCGAT 1 cut(s) 240
BsuRI GGCC 2 cut(s) 118, 528
BsuTUI ATCGAT 1 cut(s) 240
BtsCI GGATG 2 cut(s) 529, 610
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 2 cut(s) 283, 598
BveI ACCTGC 1 cut(s) 260
Cac8I GCNNGC 1 cut(s) 439
Cfr13I GGNCC 3 cut(s) 117, 247, 386
Cfr9I CCCGGG 1 cut(s) 120
ClaI ATCGAT 1 cut(s) 240
Csp6I GTAC 3 cut(s) 146, 288, 359
CviAII CATG 1 cut(s) 203
CviJI RGCY 8 cut(s) 18, 84, 118, 406, 441, 500, 528, 593
CviKI_1 RGCY 8 cut(s) 18, 84, 118, 406, 441, 500, 528, 593
CviQI GTAC 3 cut(s) 146, 288, 359
DdeI CTNAG 1 cut(s) 80
DpnI GATC 4 cut(s) 73, 128, 239, 257
DpnII GATC 4 cut(s) 71, 126, 237, 255
EaeI YGGCCR 1 cut(s) 526
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
Eco47I GGWCC 2 cut(s) 247, 386
Eco88I CYCGRG 1 cut(s) 120
Eco91I GGTNACC 1 cut(s) 608
EcoO109I RGGNCCY 2 cut(s) 117, 247
EcoO65I GGTNACC 1 cut(s) 608
EcoRI GAATTC 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 532
FaeI CATG 1 cut(s) 206
FaiI YATR 8 cut(s) 204, 338, 350, 352, 354, 432, 473, 492
FaqI GGGAC 1 cut(s) 606
FatI CATG 1 cut(s) 202
Fnu4HI GCNGC 2 cut(s) 435, 442
FokI GGATG 2 cut(s) 536, 617
Fsp4HI GCNGC 2 cut(s) 435, 442
FspBI CTAG 1 cut(s) 114
GluI GCNGC 2 cut(s) 435, 442
GsaI CCCAGC 1 cut(s) 235
HaeIII GGCC 2 cut(s) 118, 528
HapII CCGG 2 cut(s) 121, 251
Hin1II CATG 1 cut(s) 206
HinfI GANTC 3 cut(s) 223, 323, 454
HpaII CCGG 2 cut(s) 121, 251
HphI GGTGA 1 cut(s) 620
Hpy188I TCNGA 5 cut(s) 76, 179, 328, 417, 540
HpyCH4III ACNGT 4 cut(s) 154, 200, 212, 421
HpyCH4IV ACGT 2 cut(s) 183, 637
HpyCH4V TGCA 4 cut(s) 106, 305, 395, 437
HpyF3I CTNAG 1 cut(s) 80
HpySE526I ACGT 2 cut(s) 183, 637
Hsp92II CATG 1 cut(s) 206
Kzo9I GATC 4 cut(s) 71, 126, 237, 255
LmnI GCTCC 1 cut(s) 590
Lsp1109I GCAGC 2 cut(s) 421, 428
MaeI CTAG 1 cut(s) 114
MaeII ACGT 2 cut(s) 183, 637
MaeIII GTNAC 3 cut(s) 194, 206, 608
MalI GATC 4 cut(s) 73, 128, 239, 257
MboI GATC 4 cut(s) 71, 126, 237, 255
MboII GAAGA 4 cut(s) 62, 170, 335, 470
MflI RGATCY 1 cut(s) 71
MhlI GDGCHC 1 cut(s) 34
MlsI TGGCCA 1 cut(s) 528
MluCI AATT 4 cut(s) 65, 97, 173, 577
MluNI TGGCCA 1 cut(s) 528
MmeI TCCRAC 2 cut(s) 99, 251
MnlI CCTC 5 cut(s) 134, 309, 399, 492, 541
Mox20I TGGCCA 1 cut(s) 528
MscI TGGCCA 1 cut(s) 528
MseI TTAA 2 cut(s) 68, 576
MslI CAYNNNNRTG 2 cut(s) 351, 603
Msp20I TGGCCA 1 cut(s) 528
MspI CCGG 2 cut(s) 121, 251
MspR9I CCNGG 4 cut(s) 121, 122, 251, 534
MvaI CCWGG 1 cut(s) 534
NciI CCSGG 3 cut(s) 121, 122, 251
NdeII GATC 4 cut(s) 71, 126, 237, 255
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 2 cut(s) 119, 249
NmuCI GTSAC 2 cut(s) 206, 608
NspV TTCGAA 1 cut(s) 95
PaqCI CACCTGC 1 cut(s) 260
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 3 cut(s) 223, 323, 454
PkrI GCNGC 2 cut(s) 436, 443
Ppu21I YACGTR 1 cut(s) 184
PpuMI RGGWCCY 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 532
PspEI GGTNACC 1 cut(s) 608
PspFI CCCAGC 1 cut(s) 231
PspGI CCWGG 1 cut(s) 532
PspN4I GGNNCC 2 cut(s) 119, 249
PspPI GGNCC 3 cut(s) 117, 247, 386
PspPPI RGGWCCY 1 cut(s) 247
PsuI RGATCY 1 cut(s) 71
RsaI GTAC 3 cut(s) 147, 289, 360
RsaNI GTAC 3 cut(s) 146, 288, 359
RseI CAYNNNNRTG 2 cut(s) 351, 603
SaqAI TTAA 2 cut(s) 68, 576
SatI GCNGC 2 cut(s) 435, 442
Sau3AI GATC 4 cut(s) 71, 126, 237, 255
Sau96I GGNCC 3 cut(s) 117, 247, 386
ScaI AGTACT 1 cut(s) 147
ScrFI CCNGG 4 cut(s) 121, 122, 251, 534
SduI GDGCHC 1 cut(s) 34
SfcI CTRYAG 1 cut(s) 19
SfuI TTCGAA 1 cut(s) 95
SinI GGWCC 2 cut(s) 247, 386
SmaI CCCGGG 1 cut(s) 122
SmiMI CAYNNNNRTG 2 cut(s) 351, 603
Sse9I AATT 4 cut(s) 65, 97, 173, 577
SsiI CCGC 1 cut(s) 562
SspI AATATT 2 cut(s) 133, 262
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 4 cut(s) 119, 120, 249, 532
TaaI ACNGT 4 cut(s) 154, 200, 212, 421
TaiI ACGT 2 cut(s) 186, 640
TaqI TCGA 2 cut(s) 95, 240
TasI AATT 4 cut(s) 65, 97, 173, 577
TatI WGTACW 2 cut(s) 145, 287
TfiI GAWTC 3 cut(s) 223, 323, 454
Tru1I TTAA 2 cut(s) 68, 576
Tru9I TTAA 2 cut(s) 68, 576
TscAI CASTG 2 cut(s) 283, 605
TseFI GTSAC 2 cut(s) 206, 608
TseI GCWGC 2 cut(s) 434, 441
Tsp45I GTSAC 2 cut(s) 206, 608
TspDTI ATGAA 2 cut(s) 336, 446
TspGWI ACGGA 1 cut(s) 399
TspMI CCCGGG 1 cut(s) 120
TspRI CASTG 2 cut(s) 283, 605
VpaK11BI GGWCC 2 cut(s) 247, 386
XapI RAATTY 2 cut(s) 97, 173
XmaI CCCGGG 1 cut(s) 120
XspI CTAG 1 cut(s) 114
ZrmI AGTACT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.