Rh5BG367000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008120
Physical Location & Seq
Forward (+)
57549093 .. 57552376
3284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG367000.1

Sequence Viewer

Length: 390 bp
ATGCCCACGCAGATTCGCTACAGCAGGGGAGCACGAGAAGTCCAGGTGGAAGAAGTGATTTTGAAATTAAGATCCGACCTGAGCCAGAAGAGTTCGAATTCCCTGCAAATCTCTAGGCCCCGGGAGATCAATATTCAAGGAGGAGTACTGACGGTGGAAGACTGGCAGGAGAAATTCAGATACGTGAAATCAGATTACGGTCTTGGTCACGGTCAGGGCGAGAATCCAACCCAGCAGATCGACAAGGACCCGGACGAGAATCCAACCCAGCAGATCGATAAGGACCCGGAGAACAATATTGAAGTTTTTGCATCTTTGTTTAGTACTACAACTGGTGGCAGTGGTAAAAGTACAGATGATCCTCCTGCAAGGAGTTGGGAAGATGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

129

Amino Acids

14.47

Weight (kDa)

4.7

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 66, 353
AcsI RAATTY 2 cut(s) 97, 173
AfaI GTAC 3 cut(s) 147, 325, 352
AgsI TTSAA 3 cut(s) 64, 137, 302
AjnI CCWGG 1 cut(s) 42
Alw21I GWGCWC 1 cut(s) 34
AlwI GGATC 2 cut(s) 66, 353
Ama87I CYCGRG 1 cut(s) 120
AoxI GGCC 1 cut(s) 116
ApoI RAATTY 2 cut(s) 97, 173
AspS9I GGNCC 3 cut(s) 117, 247, 283
AsuC2I CCSGG 4 cut(s) 121, 122, 251, 287
AsuII TTCGAA 1 cut(s) 95
AvaI CYCGRG 1 cut(s) 120
AvaII GGWCC 2 cut(s) 247, 283
BauI CACGAG 1 cut(s) 33
BbsI GAAGAC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 34
BccI CCATC 1 cut(s) 377
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 1 cut(s) 44
BcnI CCSGG 4 cut(s) 121, 122, 251, 287
BfaI CTAG 1 cut(s) 114
BfmI CTRYAG 1 cut(s) 19
BmcAI AGTACT 2 cut(s) 147, 325
Bme1390I CCNGG 5 cut(s) 44, 121, 122, 251, 287
Bme18I GGWCC 2 cut(s) 247, 283
BmeT110I CYCGRG 1 cut(s) 120
BmgT120I GGNCC 3 cut(s) 117, 247, 283
BmiI GGNNCC 3 cut(s) 119, 249, 285
BmrFI CCNGG 5 cut(s) 44, 121, 122, 251, 287
BmsI GCATC 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 165
Bpu10I CCTNAGC 1 cut(s) 80
Bpu14I TTCGAA 1 cut(s) 95
BpuMI CCSGG 4 cut(s) 121, 122, 251, 287
Bsa29I ATCGAT 1 cut(s) 276
BsaAI YACGTR 1 cut(s) 184
BsaJI CCNNGG 2 cut(s) 119, 120
Bse1I ACTGG 2 cut(s) 167, 337
BseBI CCWGG 1 cut(s) 44
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 2 cut(s) 119, 120
BseMII CTCAG 1 cut(s) 71
BseNI ACTGG 2 cut(s) 167, 337
BseRI GAGGAG 1 cut(s) 156
BseYI CCCAGC 2 cut(s) 231, 267
BshFI GGCC 1 cut(s) 118
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 34
BsiHKCI CYCGRG 1 cut(s) 120
BsiSI CCGG 3 cut(s) 121, 251, 287
BsnI GGCC 1 cut(s) 118
BsoBI CYCGRG 1 cut(s) 120
Bsp119I TTCGAA 1 cut(s) 95
Bsp1286I GDGCHC 1 cut(s) 34
Bsp143I GATC 5 cut(s) 71, 126, 237, 273, 358
BspANI GGCC 1 cut(s) 118
BspCNI CTCAG 1 cut(s) 72
BspDI ATCGAT 1 cut(s) 276
BspLI GGNNCC 3 cut(s) 119, 249, 285
BspPI GGATC 2 cut(s) 66, 353
BspT104I TTCGAA 1 cut(s) 95
BsrI ACTGG 2 cut(s) 167, 337
BssECI CCNNGG 2 cut(s) 119, 120
BssMI GATC 5 cut(s) 71, 126, 237, 273, 358
BssSI CACGAG 1 cut(s) 33
Bst2BI CACGAG 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 3 cut(s) 154, 200, 212
Bst6I CTCTTC 1 cut(s) 83
BstBAI YACGTR 1 cut(s) 184
BstBI TTCGAA 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 80
BstKTI GATC 5 cut(s) 74, 129, 240, 276, 361
BstMBI GATC 5 cut(s) 71, 126, 237, 273, 358
BstNI CCWGG 1 cut(s) 44
BstSCI CCNGG 5 cut(s) 42, 119, 120, 249, 285
BstSFI CTRYAG 1 cut(s) 19
BstV2I GAAGAC 1 cut(s) 165
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 1 cut(s) 118
BsuTUI ATCGAT 1 cut(s) 276
BtsI GCAGTG 1 cut(s) 346
BtsIMutI CAGTG 1 cut(s) 346
Cfr13I GGNCC 3 cut(s) 117, 247, 283
Cfr9I CCCGGG 1 cut(s) 120
ClaI ATCGAT 1 cut(s) 276
Csp6I GTAC 3 cut(s) 146, 324, 351
CviJI RGCY 2 cut(s) 84, 118
CviKI_1 RGCY 2 cut(s) 84, 118
CviQI GTAC 3 cut(s) 146, 324, 351
DdeI CTNAG 1 cut(s) 80
DpnI GATC 5 cut(s) 73, 128, 239, 275, 360
DpnII GATC 5 cut(s) 71, 126, 237, 273, 358
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco47I GGWCC 2 cut(s) 247, 283
Eco88I CYCGRG 1 cut(s) 120
EcoO109I RGGNCCY 3 cut(s) 117, 247, 283
EcoRI GAATTC 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 42
FspBI CTAG 1 cut(s) 114
GsaI CCCAGC 2 cut(s) 235, 271
HaeIII GGCC 1 cut(s) 118
HapII CCGG 3 cut(s) 121, 251, 287
HinfI GANTC 3 cut(s) 13, 223, 259
HpaII CCGG 3 cut(s) 121, 251, 287
Hpy188I TCNGA 3 cut(s) 76, 179, 193
HpyCH4III ACNGT 3 cut(s) 154, 200, 212
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 3 cut(s) 106, 311, 368
HpyF3I CTNAG 1 cut(s) 80
HpySE526I ACGT 1 cut(s) 183
Kzo9I GATC 5 cut(s) 71, 126, 237, 273, 358
LmnI GCTCC 1 cut(s) 29
LweI GCATC 1 cut(s) 320
MaeI CTAG 1 cut(s) 114
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 1 cut(s) 206
MalI GATC 5 cut(s) 73, 128, 239, 275, 360
MboI GATC 5 cut(s) 71, 126, 237, 273, 358
MboII GAAGA 3 cut(s) 62, 100, 170
MflI RGATCY 1 cut(s) 71
MhlI GDGCHC 1 cut(s) 34
MluCI AATT 3 cut(s) 65, 97, 173
MmeI TCCRAC 3 cut(s) 99, 251, 287
MnlI CCTC 2 cut(s) 134, 372
MseI TTAA 1 cut(s) 68
MspI CCGG 3 cut(s) 121, 251, 287
MspR9I CCNGG 5 cut(s) 44, 121, 122, 251, 287
MvaI CCWGG 1 cut(s) 44
NciI CCSGG 4 cut(s) 121, 122, 251, 287
NdeII GATC 5 cut(s) 71, 126, 237, 273, 358
NlaIV GGNNCC 3 cut(s) 119, 249, 285
NmuCI GTSAC 1 cut(s) 206
NspV TTCGAA 1 cut(s) 95
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 3 cut(s) 13, 223, 259
Ppu21I YACGTR 1 cut(s) 184
PpuMI RGGWCCY 2 cut(s) 247, 283
Psp5II RGGWCCY 2 cut(s) 247, 283
Psp6I CCWGG 1 cut(s) 42
PspFI CCCAGC 2 cut(s) 231, 267
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 3 cut(s) 119, 249, 285
PspPI GGNCC 3 cut(s) 117, 247, 283
PspPPI RGGWCCY 2 cut(s) 247, 283
PsuI RGATCY 1 cut(s) 71
RsaI GTAC 3 cut(s) 147, 325, 352
RsaNI GTAC 3 cut(s) 146, 324, 351
SaqAI TTAA 1 cut(s) 68
Sau3AI GATC 5 cut(s) 71, 126, 237, 273, 358
Sau96I GGNCC 3 cut(s) 117, 247, 283
ScaI AGTACT 2 cut(s) 147, 325
ScrFI CCNGG 5 cut(s) 44, 121, 122, 251, 287
SduI GDGCHC 1 cut(s) 34
SetI ASST 3 cut(s) 48, 81, 186
SfaNI GCATC 1 cut(s) 320
SfcI CTRYAG 1 cut(s) 19
SfuI TTCGAA 1 cut(s) 95
SinI GGWCC 2 cut(s) 247, 283
SmaI CCCGGG 1 cut(s) 122
Sse9I AATT 3 cut(s) 65, 97, 173
SspI AATATT 2 cut(s) 133, 298
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 5 cut(s) 42, 119, 120, 249, 285
TaaI ACNGT 3 cut(s) 154, 200, 212
TaiI ACGT 1 cut(s) 186
TaqI TCGA 3 cut(s) 95, 240, 276
TasI AATT 3 cut(s) 65, 97, 173
TatI WGTACW 3 cut(s) 145, 323, 350
TfiI GAWTC 3 cut(s) 13, 223, 259
Tru1I TTAA 1 cut(s) 68
Tru9I TTAA 1 cut(s) 68
TscAI CASTG 1 cut(s) 346
TseFI GTSAC 1 cut(s) 206
Tsp45I GTSAC 1 cut(s) 206
TspMI CCCGGG 1 cut(s) 120
TspRI CASTG 1 cut(s) 346
VpaK11BI GGWCC 2 cut(s) 247, 283
XapI RAATTY 2 cut(s) 97, 173
XmaI CCCGGG 1 cut(s) 120
XspI CTAG 1 cut(s) 114
ZrmI AGTACT 2 cut(s) 147, 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.