Rorug07G0196400

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008120
Physical Location & Seq
Forward (+)
16912343 .. 16913063
721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0196400.1

Sequence Viewer

Length: 483 bp
ATGCATGTCTTCCTTGCATCAATGACTGTCCGACTCGCAACGAAACTATCGCTCAAAGACTATGAAGAGCCAATAGACCTCGACAATCTTCGCTACCCTGGAAAAAGGTTTCTTGCACCTCAGTTCTTCCTGGTTGGACGACTGAACAGTGCCCGACTGGTGATCTTGGATTCTTTCCGGAGTGCAGTCCGCTCCATGTGGCGCCTCTCGTTGCCAATGGATGTGCAGGCTCGAGGAGATCACTTCATCTTTACGTTTGCCAACAAACGTGATATTGGACGTGTGAAGAAAGGATGCCTTTTGATCGACGAGACGATTATTCCAGTCCTACAAGTTGATGACCCCGGGATCCGATATGGTGTATTTCACTATGAGCAGCTGTTGGGGTGTTGTCGGGAATGCTCCATGATCAACCGCTGCGGTGCTCGCTGTCCTAGTGAGCAAGGGGCGGGAGAAATGCCTGAAACAGGTGGATCGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

18.07

Weight (kDa)

8.58

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 201
AccBSI CCGCTC 1 cut(s) 192
AccIII TCCGGA 1 cut(s) 177
AciI CCGC 4 cut(s) 190, 415, 420, 449
AclWI GGATC 3 cut(s) 343, 356, 481
AcyI GRCGYC 1 cut(s) 202
AfiI CCNNNNNNNGG 1 cut(s) 467
AflIII ACRYGT 1 cut(s) 280
AjiI CACGTC 1 cut(s) 281
AjnI CCWGG 2 cut(s) 97, 129
AluBI AGCT 1 cut(s) 379
AluI AGCT 1 cut(s) 379
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 1 cut(s) 305
AlwI GGATC 3 cut(s) 343, 356, 481
Ama87I CYCGRG 2 cut(s) 231, 344
Aor13HI TCCGGA 1 cut(s) 177
ApeKI GCWGC 2 cut(s) 376, 417
AspLEI GCGC 1 cut(s) 204
AsuC2I CCSGG 2 cut(s) 345, 346
AsuHPI GGTGA 1 cut(s) 172
AvaI CYCGRG 2 cut(s) 231, 344
BaeGI GKGCMC 1 cut(s) 154
BamHI GGATCC 1 cut(s) 348
BanI GGYRCC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 2 cut(s) 388, 404
BciT130I CCWGG 2 cut(s) 99, 131
BclI TGATCA 1 cut(s) 408
BcnI CCSGG 2 cut(s) 345, 346
BcoDI GTCTC 1 cut(s) 305
BfaI CTAG 1 cut(s) 435
BfoI RGCGCY 1 cut(s) 205
BisI GCNGC 2 cut(s) 377, 418
BlsI GCNGC 2 cut(s) 378, 419
Bme1390I CCNGG 4 cut(s) 99, 131, 345, 346
BmeT110I CYCGRG 2 cut(s) 231, 344
BmgBI CACGTC 1 cut(s) 281
BmiI GGNNCC 2 cut(s) 203, 350
BmrFI CCNGG 4 cut(s) 99, 131, 345, 346
BmsI GCATC 2 cut(s) 26, 284
BpuMI CCSGG 2 cut(s) 345, 346
BsaHI GRCGYC 1 cut(s) 202
BsaJI CCNNGG 3 cut(s) 97, 343, 344
BsaWI WCCGGW 1 cut(s) 177
Bsc4I CCNNNNNNNGG 1 cut(s) 467
Bse1I ACTGG 2 cut(s) 162, 323
BseAI TCCGGA 1 cut(s) 177
BseBI CCWGG 2 cut(s) 99, 131
BseDI CCNNGG 3 cut(s) 97, 343, 344
BseGI GGATG 2 cut(s) 226, 299
BseLI CCNNNNNNNGG 1 cut(s) 467
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 2 cut(s) 162, 323
BseRI GAGGAG 1 cut(s) 249
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 2 cut(s) 388, 404
BsgI GTGCAG 2 cut(s) 204, 245
BshNI GGYRCC 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 427
BsiHKCI CYCGRG 2 cut(s) 231, 344
BsiSI CCGG 2 cut(s) 178, 345
BslI CCNNNNNNNGG 1 cut(s) 467
BsmAI GTCTC 1 cut(s) 305
BsmBI CGTCTC 1 cut(s) 305
BsmI GAATGC 1 cut(s) 404
BsoBI CYCGRG 2 cut(s) 231, 344
Bsp1286I GDGCHC 2 cut(s) 154, 427
Bsp13I TCCGGA 1 cut(s) 177
Bsp143I GATC 6 cut(s) 162, 238, 303, 348, 408, 473
BspACI CCGC 4 cut(s) 190, 415, 420, 449
BspCNI CTCAG 1 cut(s) 133
BspEI TCCGGA 1 cut(s) 177
BspLI GGNNCC 2 cut(s) 203, 350
BspPI GGATC 3 cut(s) 343, 356, 481
BspQI GCTCTTC 1 cut(s) 60
BspT107I GGYRCC 1 cut(s) 201
BsrBI CCGCTC 1 cut(s) 192
BsrI ACTGG 2 cut(s) 162, 323
BssECI CCNNGG 3 cut(s) 97, 343, 344
BssMI GATC 6 cut(s) 162, 238, 303, 348, 408, 473
BssNI GRCGYC 1 cut(s) 202
Bst2UI CCWGG 2 cut(s) 99, 131
Bst4CI ACNGT 2 cut(s) 28, 149
Bst6I CTCTTC 1 cut(s) 60
BstACI GRCGYC 1 cut(s) 202
BstC8I GCNNGC 2 cut(s) 228, 427
BstDEI CTNAG 1 cut(s) 120
BstENI CCTNNNNNAGG 1 cut(s) 465
BstF5I GGATG 2 cut(s) 226, 299
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 6 cut(s) 165, 241, 306, 351, 411, 476
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 6 cut(s) 162, 238, 303, 348, 408, 473
BstMWI GCNNNNNNNGC 1 cut(s) 426
BstNI CCWGG 2 cut(s) 99, 131
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 4 cut(s) 97, 129, 343, 344
BstSLI GKGCMC 1 cut(s) 154
BstV1I GCAGC 2 cut(s) 388, 404
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BtrI CACGTC 1 cut(s) 281
BtsCI GGATG 2 cut(s) 226, 299
BtsIMutI CAGTG 1 cut(s) 154
Cac8I GCNNGC 2 cut(s) 228, 427
CfoI GCGC 1 cut(s) 204
Cfr9I CCCGGG 1 cut(s) 344
CviAII CATG 3 cut(s) 5, 196, 406
CviJI RGCY 3 cut(s) 70, 230, 379
CviKI_1 RGCY 3 cut(s) 70, 230, 379
DdeI CTNAG 1 cut(s) 120
DinI GGCGCC 1 cut(s) 203
DpnI GATC 6 cut(s) 164, 240, 305, 350, 410, 475
DpnII GATC 6 cut(s) 162, 238, 303, 348, 408, 473
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Eco88I CYCGRG 2 cut(s) 231, 344
EcoNI CCTNNNNNAGG 1 cut(s) 465
EcoRII CCWGG 2 cut(s) 97, 129
EcoT22I ATGCAT 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 203
EheI GGCGCC 1 cut(s) 203
Esp3I CGTCTC 1 cut(s) 305
FaeI CATG 3 cut(s) 8, 199, 409
FaiI YATR 6 cut(s) 6, 63, 197, 357, 372, 407
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FatI CATG 3 cut(s) 4, 195, 405
FauI CCCGC 1 cut(s) 442
FbaI TGATCA 1 cut(s) 408
Fnu4HI GCNGC 2 cut(s) 377, 418
FokI GGATG 2 cut(s) 233, 306
Fsp4HI GCNGC 2 cut(s) 377, 418
FspBI CTAG 1 cut(s) 435
GlaI GCGC 1 cut(s) 203
GluI GCNGC 2 cut(s) 377, 418
HaeII RGCGCY 1 cut(s) 205
HapII CCGG 2 cut(s) 178, 345
HhaI GCGC 1 cut(s) 204
Hin1I GRCGYC 1 cut(s) 202
Hin1II CATG 3 cut(s) 8, 199, 409
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HinfI GANTC 2 cut(s) 33, 170
HpaII CCGG 2 cut(s) 178, 345
HphI GGTGA 1 cut(s) 172
Hpy188I TCNGA 2 cut(s) 32, 353
Hpy188III TCNNGA 2 cut(s) 178, 395
Hpy99I CGWCG 1 cut(s) 311
HpyCH4III ACNGT 2 cut(s) 28, 149
HpyCH4IV ACGT 3 cut(s) 254, 268, 280
HpyCH4V TGCA 5 cut(s) 4, 17, 116, 185, 226
HpyF10VI GCNNNNNNNGC 1 cut(s) 426
HpyF3I CTNAG 1 cut(s) 120
HpySE526I ACGT 3 cut(s) 254, 268, 280
Hsp92I GRCGYC 1 cut(s) 202
Hsp92II CATG 3 cut(s) 8, 199, 409
HspAI GCGC 1 cut(s) 202
KasI GGCGCC 1 cut(s) 201
Kpn2I TCCGGA 1 cut(s) 177
Ksp22I TGATCA 1 cut(s) 408
Kzo9I GATC 6 cut(s) 162, 238, 303, 348, 408, 473
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 2 cut(s) 197, 407
Lsp1109I GCAGC 2 cut(s) 388, 404
LweI GCATC 2 cut(s) 26, 284
MaeI CTAG 1 cut(s) 435
MaeII ACGT 3 cut(s) 254, 268, 280
MalI GATC 6 cut(s) 164, 240, 305, 350, 410, 475
MbiI CCGCTC 1 cut(s) 192
MboI GATC 6 cut(s) 162, 238, 303, 348, 408, 473
MboII GAAGA 4 cut(s) 77, 80, 118, 298
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 2 cut(s) 154, 427
Mly113I GGCGCC 1 cut(s) 202
MlyI GAGTC 1 cut(s) 27
MmeI TCCRAC 2 cut(s) 55, 115
MnlI CCTC 4 cut(s) 89, 129, 215, 227
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 177
MspA1I CMGCKG 2 cut(s) 379, 417
MspI CCGG 2 cut(s) 178, 345
MspR9I CCNGG 4 cut(s) 99, 131, 345, 346
Mva1269I GAATGC 1 cut(s) 404
MvaI CCWGG 2 cut(s) 99, 131
MwoI GCNNNNNNNGC 1 cut(s) 426
NarI GGCGCC 1 cut(s) 202
NciI CCSGG 2 cut(s) 345, 346
NdeII GATC 6 cut(s) 162, 238, 303, 348, 408, 473
NlaIII CATG 3 cut(s) 8, 199, 409
NlaIV GGNNCC 2 cut(s) 203, 350
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeR7I CTCGAG 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 60
PctI GAATGC 1 cut(s) 404
PfeI GAWTC 1 cut(s) 170
PkrI GCNGC 2 cut(s) 378, 419
PleI GAGTC 1 cut(s) 27
PluTI GGCGCC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 27
Psp6I CCWGG 2 cut(s) 97, 129
PspGI CCWGG 2 cut(s) 97, 129
PspN4I GGNNCC 2 cut(s) 203, 350
PspXI VCTCGAGB 1 cut(s) 231
PsuI RGATCY 1 cut(s) 348
PvuII CAGCTG 1 cut(s) 379
SapI GCTCTTC 1 cut(s) 60
SatI GCNGC 2 cut(s) 377, 418
Sau3AI GATC 6 cut(s) 162, 238, 303, 348, 408, 473
SchI GAGTC 1 cut(s) 27
ScrFI CCNGG 4 cut(s) 99, 131, 345, 346
SduI GDGCHC 2 cut(s) 154, 427
SetI ASST 8 cut(s) 81, 110, 121, 257, 271, 283, 381, 472
SfaNI GCATC 2 cut(s) 26, 284
SfoI GGCGCC 1 cut(s) 203
Sfr274I CTCGAG 1 cut(s) 231
SlaI CTCGAG 1 cut(s) 231
SmaI CCCGGG 1 cut(s) 346
SmlI CTYRAG 1 cut(s) 231
SmoI CTYRAG 1 cut(s) 231
SsiI CCGC 4 cut(s) 190, 415, 420, 449
SspDI GGCGCC 1 cut(s) 201
SspMI CTAG 1 cut(s) 435
StyD4I CCNGG 4 cut(s) 97, 129, 343, 344
TaaI ACNGT 2 cut(s) 28, 149
TaiI ACGT 3 cut(s) 257, 271, 283
TaqI TCGA 3 cut(s) 81, 232, 306
TfiI GAWTC 1 cut(s) 170
TscAI CASTG 1 cut(s) 154
TseI GCWGC 2 cut(s) 376, 417
TspDTI ATGAA 2 cut(s) 78, 235
TspMI CCCGGG 1 cut(s) 344
TspRI CASTG 1 cut(s) 154
XagI CCTNNNNNAGG 1 cut(s) 465
XceI RCATGY 1 cut(s) 8
XhoI CTCGAG 1 cut(s) 231
XmaI CCCGGG 1 cut(s) 344
XspI CTAG 1 cut(s) 435
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.