Rh5BG016500

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008120
Physical Location & Seq
Reverse (-)
1173665 .. 1176329
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG016500.1

Sequence Viewer

Length: 483 bp
ATGAAGCCAACACAAGTTGCGGAGAAACCCCTTTCGGGATCGAGATCGAGATCCGGATCCTGGCTGGGCCAGAATCCGATCCGCTCATTCGTGAGTGACCCCAAACGAGCTCGTTTGGATGATGGCTTCTTTATTAAAATCCCGCCTCACCTGCTAGCAGATTTAGGTGATGTTGACTCGACTGACAGTGAAGCAGAGGAAGAAGAAGAAAAAGAAGGTCACGGTCGTCTTGACAGCTGTCTGATTTGTGGTCGGATTGTTAGACATCACACTGCTTACTGCCCGTACTTGAGATTAGTCCCACACAATGCAACTCTTCCTCCTGGCGTTCAAAGACTTTGCAAGTGTTGCGGTTTGAGGGATGGCCACCCTGGTAGAACAGACTGGAAAGGATTTGTTGTTATGAAGCAGTGTGACAATTGTGGTTCCTATTTAGAGCACTGGACTGATGAATGCCCAAACAAGGATCGTAATGACCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

18.0

Weight (kDa)

6.37

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 159
Acc36I ACCTGC 1 cut(s) 159
AccBSI CCGCTC 1 cut(s) 84
AccIII TCCGGA 1 cut(s) 53
AciI CCGC 4 cut(s) 20, 82, 143, 351
AclWI GGATC 6 cut(s) 45, 46, 51, 64, 73, 474
AcoI YGGCCR 1 cut(s) 364
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 3 cut(s) 35, 60, 463
AgsI TTSAA 1 cut(s) 332
AjnI CCWGG 3 cut(s) 59, 322, 370
AluBI AGCT 2 cut(s) 110, 237
AluI AGCT 2 cut(s) 110, 237
Alw21I GWGCWC 2 cut(s) 112, 441
AlwI GGATC 6 cut(s) 45, 46, 51, 64, 73, 474
Aor13HI TCCGGA 1 cut(s) 53
AoxI GGCC 2 cut(s) 67, 364
AspS9I GGNCC 1 cut(s) 67
AsuHPI GGTGA 2 cut(s) 140, 179
AsuNHI GCTAGC 1 cut(s) 154
BalI TGGCCA 1 cut(s) 366
BamHI GGATCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 112
Bbv12I GWGCWC 2 cut(s) 112, 441
BccI CCATC 2 cut(s) 116, 356
BciT130I CCWGG 3 cut(s) 61, 324, 372
BfaI CTAG 1 cut(s) 155
BfuAI ACCTGC 1 cut(s) 159
Bme1390I CCNGG 3 cut(s) 61, 324, 372
BmgT120I GGNCC 1 cut(s) 67
BmiI GGNNCC 2 cut(s) 58, 427
BmrFI CCNGG 3 cut(s) 61, 324, 372
BmtI GCTAGC 1 cut(s) 158
BoxI GACNNNNGTC 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 310
BsaBI GATNNNNATC 3 cut(s) 43, 49, 55
BsaJI CCNNGG 1 cut(s) 370
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 304, 334
Bsc4I CCNNNNNNNGG 3 cut(s) 35, 60, 463
Bse1I ACTGG 3 cut(s) 389, 446, 478
Bse8I GATNNNNATC 3 cut(s) 43, 49, 55
BseAI TCCGGA 1 cut(s) 53
BseBI CCWGG 3 cut(s) 61, 324, 372
BseDI CCNNGG 1 cut(s) 370
BseGI GGATG 2 cut(s) 124, 367
BseJI GATNNNNATC 3 cut(s) 43, 49, 55
BseLI CCNNNNNNNGG 3 cut(s) 35, 60, 463
BseNI ACTGG 3 cut(s) 389, 446, 478
BseYI CCCAGC 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 2 cut(s) 69, 366
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 2 cut(s) 112, 441
BsiSI CCGG 1 cut(s) 54
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 3 cut(s) 35, 60, 463
BsmFI GGGAC 1 cut(s) 284
BsmI GAATGC 1 cut(s) 458
BsnI GGCC 2 cut(s) 69, 366
Bsp1286I GDGCHC 2 cut(s) 112, 441
Bsp13I TCCGGA 1 cut(s) 53
Bsp143I GATC 6 cut(s) 38, 44, 50, 56, 78, 466
BspACI CCGC 4 cut(s) 20, 82, 143, 351
BspANI GGCC 2 cut(s) 69, 366
BspEI TCCGGA 1 cut(s) 53
BspLI GGNNCC 2 cut(s) 58, 427
BspMI ACCTGC 1 cut(s) 159
BspOI GCTAGC 1 cut(s) 158
BspPI GGATC 6 cut(s) 45, 46, 51, 64, 73, 474
BsrBI CCGCTC 1 cut(s) 84
BsrI ACTGG 3 cut(s) 389, 446, 478
BssECI CCNNGG 1 cut(s) 370
BssMI GATC 6 cut(s) 38, 44, 50, 56, 78, 466
Bst2UI CCWGG 3 cut(s) 61, 324, 372
Bst4CI ACNGT 2 cut(s) 188, 224
Bst6I CTCTTC 1 cut(s) 321
BstAPI GCANNNNNTGC 1 cut(s) 348
BstC8I GCNNGC 1 cut(s) 156
BstF5I GGATG 2 cut(s) 124, 367
BstKTI GATC 6 cut(s) 41, 47, 53, 59, 81, 469
BstMBI GATC 6 cut(s) 38, 44, 50, 56, 78, 466
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 2 cut(s) 151, 348
BstNI CCWGG 3 cut(s) 61, 324, 372
BstPAI GACNNNNGTC 1 cut(s) 237
BstSCI CCNGG 3 cut(s) 59, 322, 370
BstX2I RGATCY 2 cut(s) 50, 56
BstYI RGATCY 2 cut(s) 50, 56
BsuRI GGCC 2 cut(s) 69, 366
BtsCI GGATG 2 cut(s) 124, 367
BtsI GCAGTG 2 cut(s) 270, 416
BtsIMutI CAGTG 4 cut(s) 193, 270, 416, 439
BveI ACCTGC 1 cut(s) 159
Cac8I GCNNGC 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 67
Csp6I GTAC 1 cut(s) 286
CviJI RGCY 7 cut(s) 7, 64, 69, 110, 126, 237, 366
CviKI_1 RGCY 7 cut(s) 7, 64, 69, 110, 126, 237, 366
CviQI GTAC 1 cut(s) 286
DpnI GATC 6 cut(s) 40, 46, 52, 58, 80, 468
DpnII GATC 6 cut(s) 38, 44, 50, 56, 78, 466
EaeI YGGCCR 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
Ecl136II GAGCTC 1 cut(s) 110
Eco24I GRGCYC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 110
EcoICRI GAGCTC 1 cut(s) 110
EcoRII CCWGG 3 cut(s) 59, 322, 370
EcoT38I GRGCYC 1 cut(s) 112
FaiI YATR 1 cut(s) 404
FaqI GGGAC 1 cut(s) 284
FauI CCCGC 1 cut(s) 150
FokI GGATG 2 cut(s) 131, 374
FriOI GRGCYC 1 cut(s) 112
FspBI CTAG 1 cut(s) 155
GsaI CCCAGC 1 cut(s) 68
HaeIII GGCC 2 cut(s) 69, 366
HapII CCGG 1 cut(s) 54
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 2 cut(s) 73, 176
HpaII CCGG 1 cut(s) 54
HphI GGTGA 2 cut(s) 140, 179
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 3 cut(s) 78, 243, 255
Hpy188III TCNNGA 6 cut(s) 36, 42, 48, 54, 91, 230
Hpy8I GTNNAC 1 cut(s) 175
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 2 cut(s) 188, 224
HpyCH4V TGCA 2 cut(s) 311, 342
HpyF10VI GCNNNNNNNGC 2 cut(s) 151, 348
Kpn2I TCCGGA 1 cut(s) 53
Kzo9I GATC 6 cut(s) 38, 44, 50, 56, 78, 466
MaeI CTAG 1 cut(s) 155
MaeIII GTNAC 3 cut(s) 95, 218, 413
MalI GATC 6 cut(s) 40, 46, 52, 58, 80, 468
MbiI CCGCTC 1 cut(s) 84
MboI GATC 6 cut(s) 38, 44, 50, 56, 78, 466
MboII GAAGA 4 cut(s) 212, 215, 218, 308
MfeI CAATTG 1 cut(s) 418
MflI RGATCY 2 cut(s) 50, 56
MhlI GDGCHC 2 cut(s) 112, 441
MlsI TGGCCA 1 cut(s) 366
MluCI AATT 1 cut(s) 418
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 4 cut(s) 156, 190, 330, 351
Mox20I TGGCCA 1 cut(s) 366
MroI TCCGGA 1 cut(s) 53
MscI TGGCCA 1 cut(s) 366
MseI TTAA 1 cut(s) 135
Msp20I TGGCCA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 237
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 3 cut(s) 61, 324, 372
MunI CAATTG 1 cut(s) 418
Mva1269I GAATGC 1 cut(s) 458
MvaI CCWGG 3 cut(s) 61, 324, 372
MwoI GCNNNNNNNGC 2 cut(s) 151, 348
NdeII GATC 6 cut(s) 38, 44, 50, 56, 78, 466
NheI GCTAGC 1 cut(s) 154
NlaIV GGNNCC 2 cut(s) 58, 427
NmuCI GTSAC 3 cut(s) 95, 218, 413
PaqCI CACCTGC 1 cut(s) 159
PctI GAATGC 1 cut(s) 458
PfeI GAWTC 1 cut(s) 73
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PshAI GACNNNNGTC 1 cut(s) 237
Psp124BI GAGCTC 1 cut(s) 112
Psp6I CCWGG 3 cut(s) 59, 322, 370
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 3 cut(s) 59, 322, 370
PspN4I GGNNCC 2 cut(s) 58, 427
PspPI GGNCC 1 cut(s) 67
PsuI RGATCY 2 cut(s) 50, 56
PvuII CAGCTG 1 cut(s) 237
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SacI GAGCTC 1 cut(s) 112
SaqAI TTAA 1 cut(s) 135
Sau3AI GATC 6 cut(s) 38, 44, 50, 56, 78, 466
Sau96I GGNCC 1 cut(s) 67
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 3 cut(s) 61, 324, 372
SduI GDGCHC 2 cut(s) 112, 441
SetI ASST 5 cut(s) 112, 153, 169, 220, 239
SmlI CTYRAG 1 cut(s) 289
SmoI CTYRAG 1 cut(s) 289
Sse9I AATT 1 cut(s) 418
SsiI CCGC 4 cut(s) 20, 82, 143, 351
SspMI CTAG 1 cut(s) 155
SstI GAGCTC 1 cut(s) 112
StyD4I CCNGG 3 cut(s) 59, 322, 370
TaaI ACNGT 2 cut(s) 188, 224
TaqI TCGA 3 cut(s) 41, 47, 179
TasI AATT 1 cut(s) 418
TfiI GAWTC 1 cut(s) 73
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 4 cut(s) 193, 277, 416, 446
TseFI GTSAC 3 cut(s) 95, 218, 413
Tsp45I GTSAC 3 cut(s) 95, 218, 413
TspDTI ATGAA 3 cut(s) 17, 419, 465
TspRI CASTG 4 cut(s) 193, 277, 416, 446
XspI CTAG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.