MD09G1111400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008120
Physical Location & Seq
Reverse (-)
8465611 .. 8472921
7311 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1111400.v1.1.491

Sequence Viewer

Length: 348 bp
ATGAGCCAGAGCGGTAACGAGTCCGAGCGCCAGAGCAAGAAACAAAAACTCTTGAGTCCAGATCCAGAATCCCTCCAGGGCCATATCCCGGACTTACCACCGAACGCAGACGAGCTGCGCAAGCGGGGGAAGCTCATATGGCACTGGGACGATAGCGATGATGATGAATTCGAGGACTTCCCCCCGGGAAAGGTTTATTTTGCTATCGAGGAAAACTATAATGCTCGAATGACCGCGAAAAAAGAATTGAGTGAGATAAGGGCACGAAGATCATCCCTCAAAGAAAGGAAGAACACCAGTATGTCCCCCGAATTAACAAGACTAGTCAGCGGAAGCACAGACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

13.28

Weight (kDa)

5.74

Isoelectric Point (pI)

78.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 12
AccII CGCG 1 cut(s) 236
AciI CCGC 4 cut(s) 12, 124, 234, 330
AclWI GGATC 1 cut(s) 56
AcsI RAATTY 1 cut(s) 167
AfiI CCNNNNNNNGG 2 cut(s) 88, 190
AhlI ACTAGT 1 cut(s) 322
AjnI CCWGG 1 cut(s) 75
AluBI AGCT 2 cut(s) 115, 133
AluI AGCT 2 cut(s) 115, 133
AlwI GGATC 1 cut(s) 56
Ama87I CYCGRG 1 cut(s) 184
AoxI GGCC 1 cut(s) 79
ApeKI GCWGC 1 cut(s) 115
ApoI RAATTY 1 cut(s) 167
AspLEI GCGC 2 cut(s) 30, 120
AspS9I GGNCC 1 cut(s) 79
AsuC2I CCSGG 3 cut(s) 89, 185, 186
AvaI CYCGRG 1 cut(s) 184
BaeGI GKGCMC 1 cut(s) 265
BbvI GCAGC 1 cut(s) 102
BciT130I CCWGG 1 cut(s) 77
BcnI CCSGG 3 cut(s) 89, 185, 186
BcuI ACTAGT 1 cut(s) 322
BfaI CTAG 1 cut(s) 323
BfoI RGCGCY 1 cut(s) 31
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
Bme1390I CCNGG 4 cut(s) 77, 89, 185, 186
BmeT110I CYCGRG 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 79
BmrFI CCNGG 4 cut(s) 77, 89, 185, 186
BmrI ACTGGG 1 cut(s) 154
BmuI ACTGGG 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 73
BpuMI CCSGG 3 cut(s) 89, 185, 186
BsaJI CCNNGG 3 cut(s) 76, 183, 184
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 190
Bse1I ACTGG 2 cut(s) 149, 297
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 3 cut(s) 76, 183, 184
BseGI GGATG 1 cut(s) 272
BseLI CCNNNNNNNGG 2 cut(s) 88, 190
BseNI ACTGG 2 cut(s) 149, 297
BseSI GKGCMC 1 cut(s) 265
BseXI GCAGC 1 cut(s) 102
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 1 cut(s) 81
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 2 cut(s) 89, 185
BslFI GGGAC 2 cut(s) 161, 289
BslI CCNNNNNNNGG 2 cut(s) 88, 190
BsmFI GGGAC 2 cut(s) 161, 289
BsnI GGCC 1 cut(s) 81
BsoBI CYCGRG 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 265
Bsp143I GATC 2 cut(s) 61, 269
BspACI CCGC 4 cut(s) 12, 124, 234, 330
BspANI GGCC 1 cut(s) 81
BspFNI CGCG 1 cut(s) 236
BspPI GGATC 1 cut(s) 56
BsrBI CCGCTC 1 cut(s) 12
BsrI ACTGG 2 cut(s) 149, 297
BssECI CCNNGG 3 cut(s) 76, 183, 184
BssMI GATC 2 cut(s) 61, 269
Bst2UI CCWGG 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 122
BstF5I GGATG 1 cut(s) 272
BstFNI CGCG 1 cut(s) 236
BstH2I RGCGCY 1 cut(s) 31
BstHHI GCGC 2 cut(s) 30, 120
BstKTI GATC 2 cut(s) 64, 272
BstMBI GATC 2 cut(s) 61, 269
BstMWI GCNNNNNNNGC 3 cut(s) 121, 130, 139
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 4 cut(s) 75, 87, 183, 184
BstSLI GKGCMC 1 cut(s) 265
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
BsuRI GGCC 1 cut(s) 81
BtgZI GCGATG 1 cut(s) 171
BtsCI GGATG 1 cut(s) 272
BtsIMutI CAGTG 1 cut(s) 142
Cac8I GCNNGC 1 cut(s) 122
CfoI GCGC 2 cut(s) 30, 120
Cfr13I GGNCC 1 cut(s) 79
Cfr9I CCCGGG 1 cut(s) 184
CviJI RGCY 4 cut(s) 6, 81, 115, 133
CviKI_1 RGCY 4 cut(s) 6, 81, 115, 133
DpnI GATC 2 cut(s) 63, 271
DpnII GATC 2 cut(s) 61, 269
Eco88I CYCGRG 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 167
EcoRII CCWGG 1 cut(s) 75
FaiI YATR 5 cut(s) 84, 137, 139, 219, 302
FaqI GGGAC 2 cut(s) 161, 289
FauI CCCGC 1 cut(s) 117
FauNDI CATATG 1 cut(s) 137
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 1 cut(s) 259
Fsp4HI GCNGC 1 cut(s) 116
FspBI CTAG 1 cut(s) 323
FspI TGCGCA 1 cut(s) 119
GlaI GCGC 2 cut(s) 29, 119
GluI GCNGC 1 cut(s) 116
GsuI CTGGAG 1 cut(s) 59
HaeII RGCGCY 1 cut(s) 31
HaeIII GGCC 1 cut(s) 81
HapII CCGG 2 cut(s) 89, 185
HhaI GCGC 2 cut(s) 30, 120
Hin6I GCGC 2 cut(s) 28, 118
HinP1I GCGC 2 cut(s) 28, 118
HinfI GANTC 3 cut(s) 20, 55, 68
HpaII CCGG 2 cut(s) 89, 185
Hpy188I TCNGA 1 cut(s) 25
Hpy188III TCNNGA 3 cut(s) 52, 59, 65
HpyF10VI GCNNNNNNNGC 3 cut(s) 121, 130, 139
HspAI GCGC 2 cut(s) 28, 118
Kzo9I GATC 2 cut(s) 61, 269
Lsp1109I GCAGC 1 cut(s) 102
MaeI CTAG 1 cut(s) 323
MaeIII GTNAC 1 cut(s) 14
MalI GATC 2 cut(s) 63, 271
MbiI CCGCTC 1 cut(s) 12
MboI GATC 2 cut(s) 61, 269
MboII GAAGA 2 cut(s) 279, 301
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 1 cut(s) 265
MluCI AATT 3 cut(s) 167, 245, 311
MlyI GAGTC 2 cut(s) 29, 64
MnlI CCTC 4 cut(s) 83, 166, 202, 287
MseI TTAA 1 cut(s) 314
MslI CAYNNNNRTG 1 cut(s) 299
MspA1I CMGCKG 1 cut(s) 330
MspI CCGG 2 cut(s) 89, 185
MspR9I CCNGG 4 cut(s) 77, 89, 185, 186
MvaI CCWGG 1 cut(s) 77
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 3 cut(s) 121, 130, 139
NciI CCSGG 3 cut(s) 89, 185, 186
NdeI CATATG 1 cut(s) 137
NdeII GATC 2 cut(s) 61, 269
NsbI TGCGCA 1 cut(s) 119
PfeI GAWTC 1 cut(s) 68
PfoI TCCNGGA 1 cut(s) 87
PkrI GCNGC 1 cut(s) 117
PleI GAGTC 2 cut(s) 28, 63
PpsI GAGTC 2 cut(s) 28, 63
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspPI GGNCC 1 cut(s) 79
PsuI RGATCY 1 cut(s) 61
RseI CAYNNNNRTG 1 cut(s) 299
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 2 cut(s) 61, 269
Sau96I GGNCC 1 cut(s) 79
SchI GAGTC 2 cut(s) 29, 64
ScrFI CCNGG 4 cut(s) 77, 89, 185, 186
SduI GDGCHC 1 cut(s) 265
SetI ASST 3 cut(s) 117, 135, 195
SmaI CCCGGG 1 cut(s) 186
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
SpeI ACTAGT 1 cut(s) 322
Sse9I AATT 3 cut(s) 167, 245, 311
SsiI CCGC 4 cut(s) 12, 124, 234, 330
SspMI CTAG 1 cut(s) 323
StyD4I CCNGG 4 cut(s) 75, 87, 183, 184
TaqI TCGA 3 cut(s) 171, 207, 226
TasI AATT 3 cut(s) 167, 245, 311
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TscAI CASTG 1 cut(s) 149
TseI GCWGC 1 cut(s) 115
TspDTI ATGAA 1 cut(s) 180
TspMI CCCGGG 1 cut(s) 184
TspRI CASTG 1 cut(s) 149
XapI RAATTY 1 cut(s) 167
XmaI CCCGGG 1 cut(s) 184
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.