MD02G1302400.v1.1

Uncharacterized protein family, UPF0114

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005158
Physical Location & Seq
Reverse (-)
35670507 .. 35672960
2454 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1302400.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGCTCTCTTCACTTCTATCTCCACCTCAACCCTCTCCGTCTCGGCGGCAATCCGTCCCATCGGGCCGTCGAACAGCTCTAAGCGCTTCTCTCATTCCATCGCTGCTTCTTCTCTGAGCTCCCCACAGTCTCCGGAATCAAAATCAACTGCCCCAAATCAAACCGCAGTCTCCTCCTCCACTGAAACTTCACCAAACCCTTTTAATGGTGCGTCGTCAAGACCCCCAGAGCCGGCGGGCTTCAACTACGCCCTTGCCAACGGCAACCCCTTTGTTCGGTTTGCCCGTTCCGCTGAGTCTTCCATTGAAAGGACAATATTTGACTTCCGGTTTTTGGCACTGTTTGCTGTTGGAGGTTCGTTGGCTGGTTCACTGTTGTGCTTTCTCAACGGTTGTGTTTATATAATGGATGCTTACAAAGTTTATTGGACTAGTTGTGTCAAAGGGAATCACACTGGACATATGGTTCTCCGACTTGTTGAAGCTATCGATGTTTACCTTGCTGGGACTGTCATGTTAATATTTGGCATGGGACTTTACGGATTATTTATCAGTAATGTCCCTAATGATGTGCCTTCCATTGATGATCGCGCTCTAAAGGGATCTTCTTTGTTCGGGATGTTTGCTATGAAGGAGAGACCTAAATGGATGAAAATTAGTTCACTCGATGAATTGAAGACGAAAGTGGGGCACGTAATTGTCATGATTCTTCTGGTAAAGATGTTTGAGCGGAGCAAGATGGTGCAGATAACCACCGGTTTGGATCTATTGAGCTATTCTGTCTGTATTTTCCTGTCTTCTGCTTCTCTGTACGTCCTTCATAATCTACACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0114

Protein Analysis

279

Amino Acids

30.11

Weight (kDa)

9.28

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0114 PF03350 106 - 244 1.4e-34 Uncharacterized protein family, UPF0114
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 730
AccII CGCG 1 cut(s) 591
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 5 cut(s) 47, 165, 236, 291, 730
AclWI GGATC 2 cut(s) 610, 771
AfaI GTAC 1 cut(s) 812
AfeI AGCGCT 1 cut(s) 86
AfiI CCNNNNNNNGG 5 cut(s) 206, 232, 276, 309, 334
AgeI ACCGGT 1 cut(s) 755
AgsI TTSAA 4 cut(s) 244, 308, 482, 676
AhlI ACTAGT 1 cut(s) 431
AleI CACNNNNGTG 1 cut(s) 376
AloI GAACNNNNNNTCC 2 cut(s) 450, 482
AluBI AGCT 4 cut(s) 78, 120, 485, 774
AluI AGCT 4 cut(s) 78, 120, 485, 774
Alw21I GWGCWC 1 cut(s) 122
Alw26I GTCTC 4 cut(s) 46, 135, 175, 631
AlwI GGATC 2 cut(s) 610, 771
Aor13HI TCCGGA 1 cut(s) 133
Aor51HI AGCGCT 1 cut(s) 86
AoxI GGCC 1 cut(s) 65
ApeKI GCWGC 1 cut(s) 104
AsiGI ACCGGT 1 cut(s) 755
AspLEI GCGC 2 cut(s) 87, 593
AspS9I GGNCC 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 183
BaeGI GKGCMC 1 cut(s) 693
BanII GRGCYC 1 cut(s) 122
BbsI GAAGAC 3 cut(s) 291, 683, 789
Bbv12I GWGCWC 1 cut(s) 122
BbvI GCAGC 1 cut(s) 91
BccI CCATC 3 cut(s) 68, 107, 733
BceAI ACGGC 2 cut(s) 52, 277
BcoDI GTCTC 4 cut(s) 46, 135, 175, 631
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 1 cut(s) 432
BfoI RGCGCY 1 cut(s) 88
BisI GCNGC 2 cut(s) 48, 105
BlsI GCNGC 2 cut(s) 49, 106
BmgT120I GGNCC 1 cut(s) 65
BmsI GCATC 1 cut(s) 400
BpiI GAAGAC 3 cut(s) 291, 683, 789
Bsa29I ATCGAT 1 cut(s) 489
BsaAI YACGTR 1 cut(s) 694
BsaI GGTCTC 1 cut(s) 631
BsaWI WCCGGW 3 cut(s) 133, 327, 755
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 232, 276, 309, 334
Bse118I RCCGGY 2 cut(s) 232, 755
Bse1I ACTGG 1 cut(s) 460
BseAI TCCGGA 1 cut(s) 133
BseCI ATCGAT 1 cut(s) 489
BseGI GGATG 3 cut(s) 415, 624, 654
BseLI CCNNNNNNNGG 5 cut(s) 206, 232, 276, 309, 334
BseMII CTCAG 2 cut(s) 107, 285
BseNI ACTGG 1 cut(s) 460
BseRI GAGGAG 2 cut(s) 163, 166
BseSI GKGCMC 1 cut(s) 693
BseXI GCAGC 1 cut(s) 91
BseYI CCCAGC 1 cut(s) 503
BsgI GTGCAG 1 cut(s) 764
Bsh1236I CGCG 1 cut(s) 591
BshFI GGCC 1 cut(s) 67
BshTI ACCGGT 1 cut(s) 755
BshVI ATCGAT 1 cut(s) 489
BsiHKAI GWGCWC 1 cut(s) 122
BsiSI CCGG 4 cut(s) 134, 233, 328, 756
BslFI GGGAC 4 cut(s) 42, 520, 545, 546
BslI CCNNNNNNNGG 5 cut(s) 206, 232, 276, 309, 334
BsmAI GTCTC 4 cut(s) 46, 135, 175, 631
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 4 cut(s) 42, 520, 545, 546
BsnI GGCC 1 cut(s) 67
Bso31I GGTCTC 1 cut(s) 631
Bsp1286I GDGCHC 2 cut(s) 122, 693
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 3 cut(s) 586, 602, 763
BspACI CCGC 5 cut(s) 47, 165, 236, 291, 730
BspANI GGCC 1 cut(s) 67
BspCNI CTCAG 2 cut(s) 108, 286
BspDI ATCGAT 1 cut(s) 489
BspEI TCCGGA 1 cut(s) 133
BspFNI CGCG 1 cut(s) 591
BspHI TCATGA 1 cut(s) 702
BspPI GGATC 2 cut(s) 610, 771
BspTNI GGTCTC 1 cut(s) 631
BsrBI CCGCTC 1 cut(s) 730
BsrFI RCCGGY 2 cut(s) 232, 755
BsrI ACTGG 1 cut(s) 460
BssAI RCCGGY 2 cut(s) 232, 755
BssMI GATC 3 cut(s) 586, 602, 763
Bst4CI ACNGT 5 cut(s) 129, 342, 375, 392, 511
Bst6I CTCTTC 1 cut(s) 14
BstAPI GCANNNNNTGC 1 cut(s) 344
BstBAI YACGTR 1 cut(s) 694
BstC8I GCNNGC 2 cut(s) 234, 238
BstDEI CTNAG 3 cut(s) 81, 116, 294
BstF5I GGATG 3 cut(s) 415, 624, 654
BstFNI CGCG 1 cut(s) 591
BstH2I RGCGCY 1 cut(s) 88
BstHHI GCGC 2 cut(s) 87, 593
BstKTI GATC 3 cut(s) 589, 605, 766
BstMAI GTCTC 4 cut(s) 46, 135, 175, 631
BstMBI GATC 3 cut(s) 586, 602, 763
BstMWI GCNNNNNNNGC 3 cut(s) 84, 290, 344
BstSLI GKGCMC 1 cut(s) 693
BstUI CGCG 1 cut(s) 591
BstV1I GCAGC 1 cut(s) 91
BstV2I GAAGAC 3 cut(s) 291, 683, 789
BstX2I RGATCY 2 cut(s) 602, 763
BstXI CCANNNNNNTGG 1 cut(s) 760
BstYI RGATCY 2 cut(s) 602, 763
Bsu15I ATCGAT 1 cut(s) 489
BsuRI GGCC 1 cut(s) 67
BsuTUI ATCGAT 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 3 cut(s) 415, 624, 654
BtsIMutI CAGTG 4 cut(s) 180, 338, 371, 453
Cac8I GCNNGC 2 cut(s) 234, 238
CciI TCATGA 1 cut(s) 702
CfoI GCGC 2 cut(s) 87, 593
Cfr10I RCCGGY 2 cut(s) 232, 755
Cfr13I GGNCC 1 cut(s) 65
ClaI ATCGAT 1 cut(s) 489
CseI GACGC 1 cut(s) 201
Csp6I GTAC 1 cut(s) 811
CspAI ACCGGT 1 cut(s) 755
CviAII CATG 3 cut(s) 514, 529, 703
CviJI RGCY 9 cut(s) 5, 67, 78, 120, 232, 240, 365, 485, 774
CviKI_1 RGCY 9 cut(s) 5, 67, 78, 120, 232, 240, 365, 485, 774
CviQI GTAC 1 cut(s) 811
DdeI CTNAG 3 cut(s) 81, 116, 294
DpnI GATC 3 cut(s) 588, 604, 765
DpnII GATC 3 cut(s) 586, 602, 763
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Ecl136II GAGCTC 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 122
Eco31I GGTCTC 1 cut(s) 631
Eco47III AGCGCT 1 cut(s) 86
Eco53kI GAGCTC 1 cut(s) 120
EcoICRI GAGCTC 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 122
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 3 cut(s) 517, 532, 706
FaiI YATR 9 cut(s) 402, 404, 462, 464, 515, 530, 629, 704, 822
FaqI GGGAC 4 cut(s) 42, 520, 545, 546
FatI CATG 3 cut(s) 513, 528, 702
FauI CCCGC 1 cut(s) 229
FauNDI CATATG 1 cut(s) 462
Fnu4HI GCNGC 2 cut(s) 48, 105
FokI GGATG 3 cut(s) 422, 631, 661
FriOI GRGCYC 1 cut(s) 122
Fsp4HI GCNGC 2 cut(s) 48, 105
FspBI CTAG 1 cut(s) 432
GlaI GCGC 2 cut(s) 86, 592
GluI GCNGC 2 cut(s) 48, 105
GsaI CCCAGC 1 cut(s) 507
HaeII RGCGCY 1 cut(s) 88
HaeIII GGCC 1 cut(s) 67
HapII CCGG 4 cut(s) 134, 233, 328, 756
HgaI GACGC 1 cut(s) 201
HhaI GCGC 2 cut(s) 87, 593
Hin1II CATG 3 cut(s) 517, 532, 706
Hin6I GCGC 2 cut(s) 85, 591
HinP1I GCGC 2 cut(s) 85, 591
HinfI GANTC 4 cut(s) 137, 296, 448, 706
HpaII CCGG 4 cut(s) 134, 233, 328, 756
HphI GGTGA 1 cut(s) 183
Hpy166II GTNNAC 3 cut(s) 371, 496, 662
Hpy188I TCNGA 2 cut(s) 117, 473
Hpy188III TCNNGA 4 cut(s) 134, 219, 616, 703
Hpy8I GTNNAC 3 cut(s) 371, 496, 662
Hpy99I CGWCG 2 cut(s) 73, 217
HpyAV CCTTC 3 cut(s) 585, 625, 827
HpyCH4III ACNGT 5 cut(s) 129, 342, 375, 392, 511
HpyCH4IV ACGT 2 cut(s) 693, 813
HpyCH4V TGCA 1 cut(s) 745
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 290, 344
HpyF3I CTNAG 3 cut(s) 81, 116, 294
HpySE526I ACGT 2 cut(s) 693, 813
Hsp92II CATG 3 cut(s) 517, 532, 706
HspAI GCGC 2 cut(s) 85, 591
Kpn2I TCCGGA 1 cut(s) 133
KroI GCCGGC 1 cut(s) 232
KroNI GCCGGC 1 cut(s) 234
Kzo9I GATC 3 cut(s) 586, 602, 763
LmnI GCTCC 2 cut(s) 125, 732
Lsp1109I GCAGC 1 cut(s) 91
LweI GCATC 1 cut(s) 400
MaeI CTAG 1 cut(s) 432
MaeII ACGT 2 cut(s) 693, 813
MalI GATC 3 cut(s) 588, 604, 765
MbiI CCGCTC 1 cut(s) 730
MboI GATC 3 cut(s) 586, 602, 763
MboII GAAGA 6 cut(s) 102, 291, 597, 688, 701, 789
MflI RGATCY 2 cut(s) 602, 763
MhlI GDGCHC 2 cut(s) 122, 693
MluCI AATT 3 cut(s) 654, 671, 696
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 2 cut(s) 331, 496
MnlI CCTC 5 cut(s) 37, 44, 184, 187, 347
MroI TCCGGA 1 cut(s) 133
MroNI GCCGGC 1 cut(s) 232
MseI TTAA 2 cut(s) 204, 518
MslI CAYNNNNRTG 1 cut(s) 376
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 4 cut(s) 134, 233, 328, 756
MvnI CGCG 1 cut(s) 591
MwoI GCNNNNNNNGC 3 cut(s) 84, 290, 344
NaeI GCCGGC 1 cut(s) 234
NdeI CATATG 1 cut(s) 462
NdeII GATC 3 cut(s) 586, 602, 763
NgoMIV GCCGGC 1 cut(s) 232
NlaIII CATG 3 cut(s) 517, 532, 706
NmeAIII GCCGAG 1 cut(s) 23
OliI CACNNNNGTG 1 cut(s) 376
PagI TCATGA 1 cut(s) 702
PcsI WCGNNNNNNNCGW 1 cut(s) 283
PdiI GCCGGC 1 cut(s) 234
PfeI GAWTC 3 cut(s) 137, 448, 706
PinAI ACCGGT 1 cut(s) 755
PkrI GCNGC 2 cut(s) 49, 106
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
Ppu21I YACGTR 1 cut(s) 694
Psp124BI GAGCTC 1 cut(s) 122
PspFI CCCAGC 1 cut(s) 503
PspPI GGNCC 1 cut(s) 65
PsuI RGATCY 2 cut(s) 602, 763
RsaI GTAC 1 cut(s) 812
RsaNI GTAC 1 cut(s) 811
RseI CAYNNNNRTG 1 cut(s) 376
SacI GAGCTC 1 cut(s) 122
SaqAI TTAA 2 cut(s) 204, 518
SatI GCNGC 2 cut(s) 48, 105
Sau3AI GATC 3 cut(s) 586, 602, 763
Sau96I GGNCC 1 cut(s) 65
SchI GAGTC 1 cut(s) 305
SduI GDGCHC 2 cut(s) 122, 693
SfaNI GCATC 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 376
SpeI ACTAGT 1 cut(s) 431
Sse9I AATT 3 cut(s) 654, 671, 696
SsiI CCGC 5 cut(s) 47, 165, 236, 291, 730
SspI AATATT 2 cut(s) 318, 522
SspMI CTAG 1 cut(s) 432
SstI GAGCTC 1 cut(s) 122
TaaI ACNGT 5 cut(s) 129, 342, 375, 392, 511
TaiI ACGT 2 cut(s) 696, 816
TaqI TCGA 3 cut(s) 71, 489, 666
TasI AATT 3 cut(s) 654, 671, 696
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 3 cut(s) 137, 448, 706
Tru1I TTAA 2 cut(s) 204, 518
Tru9I TTAA 2 cut(s) 204, 518
TscAI CASTG 4 cut(s) 187, 345, 378, 460
TseI GCWGC 1 cut(s) 104
TspDTI ATGAA 4 cut(s) 644, 665, 684, 809
TspGWI ACGGA 3 cut(s) 28, 44, 555
TspRI CASTG 4 cut(s) 187, 345, 378, 460
XspI CTAG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.