MD07G1021700.v1.1

Uncharacterized protein family, UPF0114

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005158
Physical Location & Seq
Forward (+)
1836425 .. 1838882
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1021700.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGCTCTCTTCACTTCTATCTCCTCCTCAACCCTCTGCCTCTCCACCTCAATCCATCCCATCGGGCTGTCGAACACCTCTAAACGGTTCTCTCGTCCCATCGCTGCTTCTTCTCTGAGCTCCCCACCGTCTCCGGAATCAAAATCAACGGCCCCAAAACAAACCGTAGTCTCCTCCTCCACTGAAACTTCACCAAACCATTTTAATGGTGCGTCCTCTAGACCCCCCGAGCCGGCGGGCTTCAACTACGCCCCTTCCAACGGCAACCCCTTTGTCCGGTTTGCCCGTTCCGCTGAGTCTTCCATTGAAAGGACAATATTTGACTTCCGGTTTTTGGCACTGTTTGCTGTTGGAGGTTCGTTGGCTGGTTCGTTGTTGTGCTTTCTCAATGGTTGTGTTTATATAATGGATGCGTATAAAGTTTACTGGACTAGTTGTGTCAAAGGGATTCACACTGGACGTATGGTTCTACGACTTGTTGAAGCTATCGATGTTTACCTTGCTGGGACAGTCATGCTAATATTTGGTATGGGACTGTATGGATTATTTATCAGTAATGTCCCTCATGATGTGCCTTCCATTGATGATCGTGCTCTTAAGGGCTCTTCTTTGTTCGGAATGTTTTCTCTGAAGGAAAGACCTAAATGGATGAAAATTAGCTCACTTGATGAACTGAAGACGAAGGTGGGACACGTCATTGTCATGATTCTTCTGGTTAAGATGTTTGAGAGGAGCAAGATGGTACAGATAACCACTGGTTTGGATCTGTTGAGTTATTCTGTCTGTATTTTCCTGTCTTCAGCTTCTTTGTACATCCTTCATAATCTGCACAGGGAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0114

Protein Analysis

281

Amino Acids

30.53

Weight (kDa)

9.32

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0114 PF03350 106 - 244 4e-34 Uncharacterized protein family, UPF0114
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 2 cut(s) 236, 291
AclWI GGATC 1 cut(s) 771
AcuI CTGAAG 3 cut(s) 650, 695, 783
AfaI GTAC 2 cut(s) 744, 812
AfiI CCNNNNNNNGG 6 cut(s) 84, 232, 260, 276, 309, 334
AflII CTTAAG 1 cut(s) 596
AflIII ACRYGT 1 cut(s) 691
AgsI TTSAA 3 cut(s) 244, 308, 482
AhlI ACTAGT 1 cut(s) 431
AjiI CACGTC 1 cut(s) 694
AloI GAACNNNNNNTCC 2 cut(s) 450, 482
AluBI AGCT 4 cut(s) 120, 485, 660, 803
AluI AGCT 4 cut(s) 120, 485, 660, 803
Alw21I GWGCWC 2 cut(s) 122, 595
Alw26I GTCTC 2 cut(s) 135, 175
AlwI GGATC 1 cut(s) 771
Ama87I CYCGRG 1 cut(s) 227
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 1 cut(s) 150
ApeKI GCWGC 1 cut(s) 104
Asp700I GAANNNNTTC 1 cut(s) 622
AspS9I GGNCC 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 183
AvaI CYCGRG 1 cut(s) 227
BanII GRGCYC 2 cut(s) 122, 605
BbsI GAAGAC 3 cut(s) 291, 683, 789
Bbv12I GWGCWC 2 cut(s) 122, 595
BbvI GCAGC 1 cut(s) 91
BccI CCATC 4 cut(s) 63, 68, 107, 733
BceAI ACGGC 2 cut(s) 165, 277
BcoDI GTCTC 2 cut(s) 135, 175
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 2 cut(s) 219, 432
BfrI CTTAAG 1 cut(s) 596
BisI GCNGC 1 cut(s) 105
BlsI GCNGC 1 cut(s) 106
BmeT110I CYCGRG 1 cut(s) 227
BmgBI CACGTC 1 cut(s) 694
BmgT120I GGNCC 1 cut(s) 151
BmiI GGNNCC 1 cut(s) 153
BmsI GCATC 1 cut(s) 400
BpiI GAAGAC 3 cut(s) 291, 683, 789
Bsa29I ATCGAT 1 cut(s) 489
BsaWI WCCGGW 3 cut(s) 133, 276, 327
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 232, 260, 276, 309, 334
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 3 cut(s) 431, 460, 760
BseAI TCCGGA 1 cut(s) 133
BseCI ATCGAT 1 cut(s) 489
BseGI GGATG 4 cut(s) 55, 415, 654, 813
BseLI CCNNNNNNNGG 6 cut(s) 84, 232, 260, 276, 309, 334
BseMII CTCAG 2 cut(s) 107, 285
BseNI ACTGG 3 cut(s) 431, 460, 760
BseRI GAGGAG 5 cut(s) 13, 16, 163, 166, 745
BseXI GCAGC 1 cut(s) 91
BseYI CCCAGC 1 cut(s) 503
BsgI GTGCAG 1 cut(s) 812
BshFI GGCC 1 cut(s) 152
BshVI ATCGAT 1 cut(s) 489
BsiHKAI GWGCWC 2 cut(s) 122, 595
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 4 cut(s) 134, 233, 277, 328
BslFI GGGAC 5 cut(s) 81, 520, 545, 546, 702
BslI CCNNNNNNNGG 6 cut(s) 84, 232, 260, 276, 309, 334
BsmAI GTCTC 2 cut(s) 135, 175
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 5 cut(s) 81, 520, 545, 546, 702
BsnI GGCC 1 cut(s) 152
BsoBI CYCGRG 1 cut(s) 227
Bsp1286I GDGCHC 3 cut(s) 122, 595, 605
Bsp13I TCCGGA 1 cut(s) 133
Bsp1407I TGTACA 1 cut(s) 810
Bsp143I GATC 2 cut(s) 586, 763
BspACI CCGC 2 cut(s) 236, 291
BspANI GGCC 1 cut(s) 152
BspCNI CTCAG 2 cut(s) 108, 286
BspDI ATCGAT 1 cut(s) 489
BspEI TCCGGA 1 cut(s) 133
BspHI TCATGA 2 cut(s) 565, 702
BspLI GGNNCC 1 cut(s) 153
BspPI GGATC 1 cut(s) 771
BspQI GCTCTTC 1 cut(s) 610
BspTI CTTAAG 1 cut(s) 596
BsrFI RCCGGY 1 cut(s) 232
BsrGI TGTACA 1 cut(s) 810
BsrI ACTGG 3 cut(s) 431, 460, 760
BssAI RCCGGY 1 cut(s) 232
BssMI GATC 2 cut(s) 586, 763
Bst4CI ACNGT 6 cut(s) 87, 129, 166, 342, 511, 537
Bst6I CTCTTC 2 cut(s) 14, 610
BstAFI CTTAAG 1 cut(s) 596
BstAPI GCANNNNNTGC 1 cut(s) 344
BstAUI TGTACA 1 cut(s) 810
BstC8I GCNNGC 2 cut(s) 234, 238
BstDEI CTNAG 2 cut(s) 116, 294
BstF5I GGATG 4 cut(s) 55, 415, 654, 813
BstKTI GATC 2 cut(s) 589, 766
BstMAI GTCTC 2 cut(s) 135, 175
BstMBI GATC 2 cut(s) 586, 763
BstMWI GCNNNNNNNGC 2 cut(s) 290, 344
BstV1I GCAGC 1 cut(s) 91
BstV2I GAAGAC 3 cut(s) 291, 683, 789
BstX2I RGATCY 1 cut(s) 763
BstXI CCANNNNNNTGG 2 cut(s) 206, 760
BstYI RGATCY 1 cut(s) 763
Bsu15I ATCGAT 1 cut(s) 489
BsuRI GGCC 1 cut(s) 152
BsuTUI ATCGAT 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 85
BtrI CACGTC 1 cut(s) 694
BtsCI GGATG 4 cut(s) 55, 415, 654, 813
BtsIMutI CAGTG 4 cut(s) 180, 338, 453, 753
Cac8I GCNNGC 2 cut(s) 234, 238
CciI TCATGA 2 cut(s) 565, 702
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 151
ClaI ATCGAT 1 cut(s) 489
CseI GACGC 1 cut(s) 201
Csp6I GTAC 2 cut(s) 743, 811
CviAII CATG 3 cut(s) 514, 566, 703
CviQI GTAC 2 cut(s) 743, 811
DdeI CTNAG 2 cut(s) 116, 294
DpnI GATC 2 cut(s) 588, 765
DpnII GATC 2 cut(s) 586, 763
Eam1104I CTCTTC 2 cut(s) 14, 610
EarI CTCTTC 2 cut(s) 14, 610
Ecl136II GAGCTC 1 cut(s) 120
Eco24I GRGCYC 2 cut(s) 122, 605
Eco53kI GAGCTC 1 cut(s) 120
Eco57I CTGAAG 3 cut(s) 650, 695, 783
Eco88I CYCGRG 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 120
EcoT38I GRGCYC 2 cut(s) 122, 605
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 3 cut(s) 517, 569, 706
FaqI GGGAC 5 cut(s) 81, 520, 545, 546, 702
FatI CATG 3 cut(s) 513, 565, 702
FauI CCCGC 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 105
FokI GGATG 4 cut(s) 42, 422, 661, 800
FriOI GRGCYC 2 cut(s) 122, 605
Fsp4HI GCNGC 1 cut(s) 105
FspBI CTAG 2 cut(s) 219, 432
GluI GCNGC 1 cut(s) 105
GsaI CCCAGC 1 cut(s) 507
HaeIII GGCC 1 cut(s) 152
HapII CCGG 4 cut(s) 134, 233, 277, 328
HgaI GACGC 1 cut(s) 201
Hin1II CATG 3 cut(s) 517, 569, 706
HinfI GANTC 4 cut(s) 137, 296, 448, 706
HpaII CCGG 4 cut(s) 134, 233, 277, 328
HphI GGTGA 1 cut(s) 183
Hpy166II GTNNAC 2 cut(s) 424, 496
Hpy188I TCNGA 3 cut(s) 117, 617, 630
Hpy188III TCNNGA 4 cut(s) 134, 219, 566, 703
Hpy8I GTNNAC 2 cut(s) 424, 496
HpyAV CCTTC 6 cut(s) 264, 585, 625, 676, 827, 830
HpyCH4III ACNGT 6 cut(s) 87, 129, 166, 342, 511, 537
HpyCH4IV ACGT 2 cut(s) 460, 693
HpyCH4V TGCA 1 cut(s) 829
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 344
HpyF3I CTNAG 2 cut(s) 116, 294
HpySE526I ACGT 2 cut(s) 460, 693
Hsp92II CATG 3 cut(s) 517, 569, 706
Kpn2I TCCGGA 1 cut(s) 133
KroI GCCGGC 1 cut(s) 232
KroNI GCCGGC 1 cut(s) 234
Kzo9I GATC 2 cut(s) 586, 763
LguI GCTCTTC 1 cut(s) 610
LmnI GCTCC 2 cut(s) 125, 732
Lsp1109I GCAGC 1 cut(s) 91
LweI GCATC 1 cut(s) 400
MaeI CTAG 2 cut(s) 219, 432
MaeII ACGT 2 cut(s) 460, 693
MalI GATC 2 cut(s) 588, 765
MboI GATC 2 cut(s) 586, 763
MboII GAAGA 6 cut(s) 102, 291, 597, 688, 701, 789
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 3 cut(s) 122, 595, 605
MluCI AATT 1 cut(s) 654
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 2 cut(s) 282, 331
MroI TCCGGA 1 cut(s) 133
MroNI GCCGGC 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 622
MseI TTAA 3 cut(s) 204, 597, 717
MslI CAYNNNNRTG 2 cut(s) 204, 701
MspA1I CMGCKG 1 cut(s) 293
MspCI CTTAAG 1 cut(s) 596
MspI CCGG 4 cut(s) 134, 233, 277, 328
MwoI GCNNNNNNNGC 2 cut(s) 290, 344
NaeI GCCGGC 1 cut(s) 234
NdeII GATC 2 cut(s) 586, 763
NgoMIV GCCGGC 1 cut(s) 232
NlaIII CATG 3 cut(s) 517, 569, 706
NlaIV GGNNCC 1 cut(s) 153
PagI TCATGA 2 cut(s) 565, 702
PciSI GCTCTTC 1 cut(s) 610
PcsI WCGNNNNNNNCGW 1 cut(s) 91
PdiI GCCGGC 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 622
PfeI GAWTC 3 cut(s) 137, 448, 706
PkrI GCNGC 1 cut(s) 106
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
Psp124BI GAGCTC 1 cut(s) 122
PspFI CCCAGC 1 cut(s) 503
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 1 cut(s) 151
PsuI RGATCY 1 cut(s) 763
RsaI GTAC 2 cut(s) 744, 812
RsaNI GTAC 2 cut(s) 743, 811
RseI CAYNNNNRTG 2 cut(s) 204, 701
SacI GAGCTC 1 cut(s) 122
SapI GCTCTTC 1 cut(s) 610
SaqAI TTAA 3 cut(s) 204, 597, 717
SatI GCNGC 1 cut(s) 105
Sau3AI GATC 2 cut(s) 586, 763
Sau96I GGNCC 1 cut(s) 151
SchI GAGTC 1 cut(s) 305
SduI GDGCHC 3 cut(s) 122, 595, 605
SfaNI GCATC 1 cut(s) 400
SmiMI CAYNNNNRTG 2 cut(s) 204, 701
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
SpeI ACTAGT 1 cut(s) 431
Sse9I AATT 1 cut(s) 654
SsiI CCGC 2 cut(s) 236, 291
SspI AATATT 2 cut(s) 318, 522
SspMI CTAG 2 cut(s) 219, 432
SstI GAGCTC 1 cut(s) 122
TaaI ACNGT 6 cut(s) 87, 129, 166, 342, 511, 537
TaiI ACGT 2 cut(s) 463, 696
TaqI TCGA 2 cut(s) 71, 489
TasI AATT 1 cut(s) 654
TatI WGTACW 1 cut(s) 810
TfiI GAWTC 3 cut(s) 137, 448, 706
Tru1I TTAA 3 cut(s) 204, 597, 717
Tru9I TTAA 3 cut(s) 204, 597, 717
TscAI CASTG 4 cut(s) 187, 345, 460, 760
TseI GCWGC 1 cut(s) 104
TspDTI ATGAA 3 cut(s) 665, 684, 809
TspRI CASTG 4 cut(s) 187, 345, 460, 760
Vha464I CTTAAG 1 cut(s) 596
XbaI TCTAGA 1 cut(s) 218
XmnI GAANNNNTTC 1 cut(s) 622
XspI CTAG 2 cut(s) 219, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.