RLG00000030579

Uncharacterized protein family, UPF0114

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005158
Physical Location & Seq
Reverse (-)
63765798 .. 63768008
2211 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030579

Sequence Viewer

Length: 558 bp
ATGGCTCTCTTCACTTCCATCTCCGCCTCCCCCTTCTCCGTCGCGTCGCCGATCGGCCGCTGCCCTTTAAACACCTTCAAGCGCTTCACTCATCCCATCATTGCTTCTTCTCTCAGCTCCCCATCTTCCCCGGAATCAAAAAAATCATCAGCTCCAAGTCAAACAGTAGTTTCCTCCACCGAAACCTCAGCAAATGCTTATCCTGCTTCCTCCAGACCGCCCGAGCCGAGCTTCAACTACGCCCTTGCCAACGGCAGCCTGTTTGCTCGTAATGTGCCTAATGATGTGCCTTCCAACGCTGATCGTGCTTTGAAGGGATCTTCTTTGTTTGGAATGTTTGCTTTGAAGGAGAGGCCTAAATGGATGAAAATTAGCTCACTTGATGAATTGAAGACAAAAGTGGGGCATGTTATTGTCATGATTCTTCTGGTAAAGATGTTTGAGAGGAGCAAGATGGTGCAGATAACCACTGGTTTGGATCTACTGAGCTATTCTGTCTGTATTTTTCTGTCTTCTGCTTCCTTGTACATCCTTCATAATCTACACAAGGAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0114

Protein Analysis

186

Amino Acids

20.02

Weight (kDa)

9.7

Isoelectric Point (pI)

59.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0114 PF03350 116 - 149 1.2e-09 Uncharacterized protein family, UPF0114
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 44
AciI CCGC 3 cut(s) 24, 58, 218
AclWI GGATC 2 cut(s) 325, 486
AcoI YGGCCR 1 cut(s) 55
AfaI GTAC 1 cut(s) 527
AfeI AGCGCT 1 cut(s) 83
AgsI TTSAA 5 cut(s) 79, 235, 313, 346, 391
AluBI AGCT 5 cut(s) 117, 152, 231, 375, 489
AluI AGCT 5 cut(s) 117, 152, 231, 375, 489
AlwI GGATC 2 cut(s) 325, 486
Ama87I CYCGRG 1 cut(s) 221
Aor51HI AGCGCT 1 cut(s) 83
AoxI GGCC 2 cut(s) 55, 353
ApeKI GCWGC 2 cut(s) 60, 255
AspLEI GCGC 1 cut(s) 84
AsuC2I CCSGG 1 cut(s) 131
AvaI CYCGRG 1 cut(s) 221
BbsI GAAGAC 2 cut(s) 398, 504
BbvCI CCTCAGC 1 cut(s) 187
BbvI GCAGC 2 cut(s) 47, 267
BccI CCATC 4 cut(s) 26, 104, 130, 448
BceAI ACGGC 1 cut(s) 268
BcnI CCSGG 1 cut(s) 131
BfoI RGCGCY 1 cut(s) 85
BisI GCNGC 3 cut(s) 58, 61, 256
BlsI GCNGC 3 cut(s) 59, 62, 257
Bme1390I CCNGG 1 cut(s) 131
BmeT110I CYCGRG 1 cut(s) 221
BmrFI CCNGG 1 cut(s) 131
BpiI GAAGAC 2 cut(s) 398, 504
BpmI CTGGAG 1 cut(s) 196
Bpu10I CCTNAGC 1 cut(s) 187
BpuMI CCSGG 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 129
Bse1I ACTGG 1 cut(s) 475
Bse3DI GCAATG 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 3 cut(s) 91, 369, 528
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 3 cut(s) 127, 201, 476
BseNI ACTGG 1 cut(s) 475
BseRI GAGGAG 1 cut(s) 460
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 2 cut(s) 47, 267
BsgI GTGCAG 1 cut(s) 479
Bsh1236I CGCG 1 cut(s) 44
Bsh1285I CGRYCG 2 cut(s) 54, 58
BshFI GGCC 2 cut(s) 57, 355
BsiEI CGRYCG 2 cut(s) 54, 58
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 1 cut(s) 131
BsnI GGCC 2 cut(s) 57, 355
BsoBI CYCGRG 1 cut(s) 221
Bsp1407I TGTACA 1 cut(s) 525
Bsp143I GATC 4 cut(s) 51, 301, 317, 478
BspACI CCGC 3 cut(s) 24, 58, 218
BspANI GGCC 2 cut(s) 57, 355
BspCNI CTCAG 3 cut(s) 126, 200, 477
BspFNI CGCG 1 cut(s) 44
BspHI TCATGA 1 cut(s) 417
BspPI GGATC 2 cut(s) 325, 486
BsrDI GCAATG 1 cut(s) 99
BsrGI TGTACA 1 cut(s) 525
BsrI ACTGG 1 cut(s) 475
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 4 cut(s) 51, 301, 317, 478
Bst4CI ACNGT 1 cut(s) 166
Bst6I CTCTTC 1 cut(s) 14
BstAUI TGTACA 1 cut(s) 525
BstDEI CTNAG 3 cut(s) 113, 187, 485
BstF5I GGATG 3 cut(s) 91, 369, 528
BstFNI CGCG 1 cut(s) 44
BstH2I RGCGCY 1 cut(s) 85
BstHHI GCGC 1 cut(s) 84
BstKTI GATC 4 cut(s) 54, 304, 320, 481
BstMBI GATC 4 cut(s) 51, 301, 317, 478
BstMCI CGRYCG 2 cut(s) 54, 58
BstMWI GCNNNNNNNGC 2 cut(s) 203, 305
BstNSI RCATGY 1 cut(s) 410
BstSCI CCNGG 1 cut(s) 129
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 2 cut(s) 47, 267
BstV2I GAAGAC 2 cut(s) 398, 504
BstX2I RGATCY 2 cut(s) 317, 478
BstXI CCANNNNNNTGG 1 cut(s) 475
BstYI RGATCY 2 cut(s) 317, 478
BstZI CGGCCG 1 cut(s) 55
BsuRI GGCC 2 cut(s) 57, 355
BtsCI GGATG 3 cut(s) 91, 369, 528
BtsIMutI CAGTG 1 cut(s) 468
CciI TCATGA 1 cut(s) 417
CfoI GCGC 1 cut(s) 84
CseI GACGC 1 cut(s) 33
Csp6I GTAC 1 cut(s) 526
CviAII CATG 2 cut(s) 407, 418
CviQI GTAC 1 cut(s) 526
DdeI CTNAG 3 cut(s) 113, 187, 485
DpnI GATC 4 cut(s) 53, 303, 319, 480
DpnII GATC 4 cut(s) 51, 301, 317, 478
DraI TTTAAA 1 cut(s) 69
EaeI YGGCCR 1 cut(s) 55
EagI CGGCCG 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
EciI GGCGGA 1 cut(s) 13
EclXI CGGCCG 1 cut(s) 55
Eco147I AGGCCT 1 cut(s) 355
Eco47III AGCGCT 1 cut(s) 83
Eco52I CGGCCG 1 cut(s) 55
Eco88I CYCGRG 1 cut(s) 221
FaeI CATG 2 cut(s) 410, 421
FaiI YATR 3 cut(s) 408, 419, 537
FatI CATG 2 cut(s) 406, 417
Fnu4HI GCNGC 3 cut(s) 58, 61, 256
FokI GGATG 3 cut(s) 78, 376, 515
Fsp4HI GCNGC 3 cut(s) 58, 61, 256
GlaI GCGC 1 cut(s) 83
GluI GCNGC 3 cut(s) 58, 61, 256
GsuI CTGGAG 1 cut(s) 196
HaeII RGCGCY 1 cut(s) 85
HaeIII GGCC 2 cut(s) 57, 355
HapII CCGG 1 cut(s) 131
HgaI GACGC 1 cut(s) 33
HhaI GCGC 1 cut(s) 84
Hin1II CATG 2 cut(s) 410, 421
Hin6I GCGC 1 cut(s) 82
HinP1I GCGC 1 cut(s) 82
HinfI GANTC 2 cut(s) 134, 421
HpaII CCGG 1 cut(s) 131
Hpy188III TCNNGA 2 cut(s) 213, 418
Hpy99I CGWCG 2 cut(s) 44, 49
HpyAV CCTTC 7 cut(s) 43, 85, 300, 307, 340, 542, 545
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4V TGCA 1 cut(s) 460
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 305
HpyF3I CTNAG 3 cut(s) 113, 187, 485
Hsp92II CATG 2 cut(s) 410, 421
HspAI GCGC 1 cut(s) 82
Kzo9I GATC 4 cut(s) 51, 301, 317, 478
LmnI GCTCC 3 cut(s) 122, 157, 447
LpnPI CCDG 6 cut(s) 144, 216, 226, 272, 413, 456
Lsp1109I GCAGC 2 cut(s) 47, 267
MalI GATC 4 cut(s) 53, 303, 319, 480
MboI GATC 4 cut(s) 51, 301, 317, 478
MboII GAAGA 6 cut(s) 99, 117, 312, 403, 416, 504
MflI RGATCY 2 cut(s) 317, 478
MluCI AATT 2 cut(s) 369, 386
MmeI TCCRAC 1 cut(s) 318
MnlI CCTC 6 cut(s) 37, 184, 196, 220, 345, 438
MseI TTAA 1 cut(s) 68
MspA1I CMGCKG 1 cut(s) 60
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 131
MvnI CGCG 1 cut(s) 44
MwoI GCNNNNNNNGC 2 cut(s) 203, 305
NciI CCSGG 1 cut(s) 131
NdeII GATC 4 cut(s) 51, 301, 317, 478
NlaIII CATG 2 cut(s) 410, 421
NmeAIII GCCGAG 1 cut(s) 252
NspI RCATGY 1 cut(s) 410
PagI TCATGA 1 cut(s) 417
PceI AGGCCT 1 cut(s) 355
PfeI GAWTC 2 cut(s) 134, 421
PkrI GCNGC 3 cut(s) 59, 62, 257
Ple19I CGATCG 1 cut(s) 54
PsuI RGATCY 2 cut(s) 317, 478
PvuI CGATCG 1 cut(s) 54
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
SaqAI TTAA 1 cut(s) 68
SatI GCNGC 3 cut(s) 58, 61, 256
Sau3AI GATC 4 cut(s) 51, 301, 317, 478
ScrFI CCNGG 1 cut(s) 131
SetI ASST 7 cut(s) 77, 119, 154, 188, 233, 377, 491
Sse9I AATT 2 cut(s) 369, 386
SseBI AGGCCT 1 cut(s) 355
SsiI CCGC 3 cut(s) 24, 58, 218
StuI AGGCCT 1 cut(s) 355
StyD4I CCNGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 166
TasI AATT 2 cut(s) 369, 386
TatI WGTACW 1 cut(s) 525
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 2 cut(s) 134, 421
Tru1I TTAA 1 cut(s) 68
Tru9I TTAA 1 cut(s) 68
TscAI CASTG 1 cut(s) 475
TseI GCWGC 2 cut(s) 60, 255
TspDTI ATGAA 3 cut(s) 380, 399, 524
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 1 cut(s) 475
XceI RCATGY 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.