Rmu_sc0000654.1_g000031

Uncharacterized protein family, UPF0114

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000654.1
OG0005158
Physical Location & Seq
Forward (+)
152315 .. 154565
2251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000654.1_g000031.1.cds

Sequence Viewer

Length: 615 bp
atggctctcttcacttccatctccgcctccccattctccgtcgcatcgccgatcggccgctgccctctaaacaccttcaagcgcttcactcatcccatcattgcttcttctctcagctccccatcttccccggaatcaaaatcatcatcagctccaagtcaaacagtagtttcctccactgaaacctcagcaaatgcttatcctgcttcctccagaccgcccgagccgagcttcaactacgcccttgccaacggcagcctgtttgctcggtttgccaagtctgctgagtcttccattgaaagaatatttatcttgctggaactgtacgtcatgctaatatttgctatgggattatatggattatttatcaataatgtgcctaacgatgagccttccagcgctgatcgttctttgaagggatcttctttgtttggaatgtttgctttgaagtggttgtggacatctctagctgttttttggccaatagatgctccagttgctaagggcctgtacaccaaggttgactttgcttggcagttggcagaacttggaagtctttgtttggaagaagtgggactaggccatgtgacaaagttattgagtagaggactttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0114

Protein Analysis

204

Amino Acids

21.97

Weight (kDa)

7.76

Isoelectric Point (pI)

54.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 24, 58, 218
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 2 cut(s) 55, 479
AfaI GTAC 2 cut(s) 326, 512
AfeI AGCGCT 2 cut(s) 83, 400
AgsI TTSAA 5 cut(s) 79, 235, 299, 415, 448
AluBI AGCT 4 cut(s) 117, 152, 231, 470
AluI AGCT 4 cut(s) 117, 152, 231, 470
AlwI GGATC 1 cut(s) 427
Ama87I CYCGRG 1 cut(s) 221
Aor51HI AGCGCT 2 cut(s) 83, 400
AoxI GGCC 4 cut(s) 55, 479, 505, 580
ApeKI GCWGC 2 cut(s) 60, 255
AspLEI GCGC 2 cut(s) 84, 401
AspS9I GGNCC 1 cut(s) 505
AsuC2I CCSGG 1 cut(s) 131
AvaI CYCGRG 1 cut(s) 221
BalI TGGCCA 1 cut(s) 481
BbsI GAAGAC 1 cut(s) 282
BbvCI CCTCAGC 1 cut(s) 187
BbvI GCAGC 2 cut(s) 47, 267
BccI CCATC 3 cut(s) 26, 104, 130
BceAI ACGGC 1 cut(s) 268
BcnI CCSGG 1 cut(s) 131
BfaI CTAG 2 cut(s) 467, 578
BfoI RGCGCY 2 cut(s) 85, 402
BisI GCNGC 3 cut(s) 58, 61, 256
BlsI GCNGC 3 cut(s) 59, 62, 257
Bme1390I CCNGG 1 cut(s) 131
BmeT110I CYCGRG 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 505
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 2 cut(s) 53, 478
BpiI GAAGAC 1 cut(s) 282
BpmI CTGGAG 2 cut(s) 196, 477
Bpu10I CCTNAGC 2 cut(s) 187, 501
BpuMI CCSGG 1 cut(s) 131
BsaJI CCNNGG 2 cut(s) 129, 516
Bse1I ACTGG 1 cut(s) 494
Bse3DI GCAATG 1 cut(s) 99
BseDI CCNNGG 2 cut(s) 129, 516
BseGI GGATG 1 cut(s) 91
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 3 cut(s) 127, 201, 276
BseNI ACTGG 1 cut(s) 494
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 2 cut(s) 47, 267
Bsh1285I CGRYCG 2 cut(s) 54, 58
BshFI GGCC 4 cut(s) 57, 481, 507, 582
BsiEI CGRYCG 2 cut(s) 54, 58
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 1 cut(s) 131
BslFI GGGAC 1 cut(s) 588
BsmFI GGGAC 1 cut(s) 588
BsnI GGCC 4 cut(s) 57, 481, 507, 582
BsoBI CYCGRG 1 cut(s) 221
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 3 cut(s) 51, 403, 419
BspACI CCGC 3 cut(s) 24, 58, 218
BspANI GGCC 4 cut(s) 57, 481, 507, 582
BspCNI CTCAG 3 cut(s) 126, 200, 277
BspPI GGATC 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 99
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 1 cut(s) 494
BssECI CCNNGG 2 cut(s) 129, 516
BssMI GATC 3 cut(s) 51, 403, 419
BssT1I CCWWGG 1 cut(s) 516
Bst4CI ACNGT 2 cut(s) 166, 324
Bst6I CTCTTC 1 cut(s) 14
BstAUI TGTACA 1 cut(s) 510
BstDEI CTNAG 4 cut(s) 113, 187, 285, 501
BstF5I GGATG 1 cut(s) 91
BstH2I RGCGCY 2 cut(s) 85, 402
BstHHI GCGC 2 cut(s) 84, 401
BstKTI GATC 3 cut(s) 54, 406, 422
BstMBI GATC 3 cut(s) 51, 403, 419
BstMCI CGRYCG 2 cut(s) 54, 58
BstMWI GCNNNNNNNGC 4 cut(s) 203, 272, 281, 497
BstSCI CCNGG 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 47, 267
BstV2I GAAGAC 1 cut(s) 282
BstX2I RGATCY 1 cut(s) 419
BstYI RGATCY 1 cut(s) 419
BstZI CGGCCG 1 cut(s) 55
BsuRI GGCC 4 cut(s) 57, 481, 507, 582
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 1 cut(s) 91
BtsIMutI CAGTG 1 cut(s) 177
CfoI GCGC 2 cut(s) 84, 401
Cfr13I GGNCC 1 cut(s) 505
Csp6I GTAC 2 cut(s) 325, 511
CviAII CATG 2 cut(s) 331, 584
CviQI GTAC 2 cut(s) 325, 511
DdeI CTNAG 4 cut(s) 113, 187, 285, 501
DpnI GATC 3 cut(s) 53, 405, 421
DpnII GATC 3 cut(s) 51, 403, 419
EaeI YGGCCR 2 cut(s) 55, 479
EagI CGGCCG 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
EciI GGCGGA 1 cut(s) 13
EclXI CGGCCG 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 516
Eco47III AGCGCT 2 cut(s) 83, 400
Eco52I CGGCCG 1 cut(s) 55
Eco88I CYCGRG 1 cut(s) 221
EcoO109I RGGNCCY 1 cut(s) 505
EcoT14I CCWWGG 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 516
FaeI CATG 2 cut(s) 334, 587
FaiI YATR 5 cut(s) 332, 347, 355, 357, 585
FalI AAGNNNNNCTT 2 cut(s) 509, 541
FaqI GGGAC 1 cut(s) 588
FatI CATG 2 cut(s) 330, 583
Fnu4HI GCNGC 3 cut(s) 58, 61, 256
FokI GGATG 1 cut(s) 78
Fsp4HI GCNGC 3 cut(s) 58, 61, 256
FspBI CTAG 2 cut(s) 467, 578
GlaI GCGC 2 cut(s) 83, 400
GluI GCNGC 3 cut(s) 58, 61, 256
GsuI CTGGAG 2 cut(s) 196, 477
HaeII RGCGCY 2 cut(s) 85, 402
HaeIII GGCC 4 cut(s) 57, 481, 507, 582
HapII CCGG 1 cut(s) 131
HhaI GCGC 2 cut(s) 84, 401
Hin1II CATG 2 cut(s) 334, 587
Hin6I GCGC 2 cut(s) 82, 399
HinP1I GCGC 2 cut(s) 82, 399
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HinfI GANTC 2 cut(s) 134, 287
HpaII CCGG 1 cut(s) 131
Hpy166II GTNNAC 3 cut(s) 459, 513, 523
Hpy188III TCNNGA 1 cut(s) 213
Hpy8I GTNNAC 3 cut(s) 459, 513, 523
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 3 cut(s) 85, 402, 409
HpyCH4III ACNGT 2 cut(s) 166, 324
HpyCH4IV ACGT 1 cut(s) 327
HpyF10VI GCNNNNNNNGC 4 cut(s) 203, 272, 281, 497
HpyF3I CTNAG 4 cut(s) 113, 187, 285, 501
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 2 cut(s) 334, 587
HspAI GCGC 2 cut(s) 82, 399
Kzo9I GATC 3 cut(s) 51, 403, 419
LmnI GCTCC 3 cut(s) 122, 157, 496
LpnPI CCDG 8 cut(s) 144, 216, 226, 272, 302, 409, 507, 521
Lsp1109I GCAGC 2 cut(s) 47, 267
LweI GCATC 2 cut(s) 53, 478
MaeI CTAG 2 cut(s) 467, 578
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 586
MalI GATC 3 cut(s) 53, 405, 421
MboI GATC 3 cut(s) 51, 403, 419
MboII GAAGA 5 cut(s) 99, 117, 282, 414, 578
MflI RGATCY 1 cut(s) 419
MlsI TGGCCA 1 cut(s) 481
MluNI TGGCCA 1 cut(s) 481
MlyI GAGTC 1 cut(s) 296
MnlI CCTC 6 cut(s) 37, 75, 184, 196, 220, 599
Mox20I TGGCCA 1 cut(s) 481
MscI TGGCCA 1 cut(s) 481
Msp20I TGGCCA 1 cut(s) 481
MspA1I CMGCKG 1 cut(s) 60
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 131
MwoI GCNNNNNNNGC 4 cut(s) 203, 272, 281, 497
NciI CCSGG 1 cut(s) 131
NdeII GATC 3 cut(s) 51, 403, 419
NlaIII CATG 2 cut(s) 334, 587
NmeAIII GCCGAG 1 cut(s) 252
NmuCI GTSAC 1 cut(s) 586
PfeI GAWTC 1 cut(s) 134
PkrI GCNGC 3 cut(s) 59, 62, 257
Ple19I CGATCG 1 cut(s) 54
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
PspPI GGNCC 1 cut(s) 505
PsuI RGATCY 1 cut(s) 419
PvuI CGATCG 1 cut(s) 54
RsaI GTAC 2 cut(s) 326, 512
RsaNI GTAC 2 cut(s) 325, 511
SatI GCNGC 3 cut(s) 58, 61, 256
Sau3AI GATC 3 cut(s) 51, 403, 419
Sau96I GGNCC 1 cut(s) 505
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 1 cut(s) 131
SetI ASST 8 cut(s) 77, 119, 154, 188, 233, 330, 472, 522
SfaNI GCATC 2 cut(s) 53, 478
SsiI CCGC 3 cut(s) 24, 58, 218
SspI AATATT 2 cut(s) 306, 339
SspMI CTAG 2 cut(s) 467, 578
StyD4I CCNGG 1 cut(s) 129
StyI CCWWGG 1 cut(s) 516
TaaI ACNGT 2 cut(s) 166, 324
TaiI ACGT 1 cut(s) 330
TatI WGTACW 1 cut(s) 510
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 1 cut(s) 134
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 2 cut(s) 60, 255
Tsp45I GTSAC 1 cut(s) 586
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 1 cut(s) 184
XspI CTAG 2 cut(s) 467, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.