Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003033

Physical Location & Seq

Genomic Coordinates Forward (+)

4532653 .. 4534560

Gene Structure 1908 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1056800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1908 bp

ATGGCTGAGAACGGCGAAGAGAAATTGTTAGCTATGGCGCGCCACATAGCCAAGACTCTGGGCAACAACGATAACATGGCCGACGATATTTTGCAGATTTTTTCCAACTTCGACGGCAGGTTCTCTCGCGAGAAATTGGCTTCCGACGACGAACGCACTCGCAGCTGCGCCGCCCTCGAGCTCTCGCTGAAGTCGCTGGACCGTCGGATCTCGCAGTATGTTGCTGCTGACCACCCAATCTGGGCCTACTCGGCCGATTCCGCGGCGTTTCTTAACTTGATCGACGACCTCATCGCCACCATACGGGACTGGACTCCCATGGCCGACGACAAGTCCGTCGGCGTTTGCCTCGCGCGTGCTGAGGATCTTATGCAGCAGGCCATGTTCCGGCTCGAGGACGAGTTCAGATCCTTGGTCGAACGCGGCGGCGAGTCACGAAAACTCAATCGCGCGTTTCATGGCGAGTCAAATGGCGCGTTGTCGTTTGAGTCGGGAGACGATGAAGAAGAAGAGGATGAGGTAATTGGGGACGGTGAGGAGCACCAGATCCCTACTGCTCAGCCGATCGGCGACTACGACATCGTGATCGATGCGCTGCCTTCTGGAACCATCAACGACCTCCATGAGATTGCTAAGCGGATGGTGGCGGCGGGGTTCGGAAAGGAGTGCTCGCACGTGTACAGCAGCTGCCGGAGAGAGTTCCTGGAAGAGAGCATGTCGAGGCTAGGCTTGCAGAAGCTGAGCATCGAAGAGGTTCAGAAGACGCCATGGCAGGACCTTGAGGACGAAATCGAGCGGTGGATCAAATCCTCAAACGTCGCGCTTCGGATTCTGTTCCCGAGCGAGCGTAGACTCTGCGATCGCATCTTCTACGGCCTCTCCTCCGCCGCAGACCTCTCTTTCATGGAGGTTTGCCGGGGCTCGACGATTCAGATACTGAACTTCGCCGACGCGGTAGCAATCGGAAGCCGATCGCCGGAGCGATTGTTCAGGATTCTCGACGTGTTCGAGACTCTGCGGGATTTGATGCCTGAATTCGAGTTAGTGTTTTCGGATCAGTACTGTTTGTTTCTCAGGAACGAAGCGAATACGATTTGGAAAAGGTTAGGGGAAGCGATCAGAGGCATTTTCATGGAGTTGGAGAATCTGATCAGTCGCGACCCTCCGAAAACTCCAGTTCCTGGCGGCGGGTTGCATCCGATCACTCGATATGTGATGAATTATCTCCGTGCAGCATGCCGATCGCGCCAGACCCTCGAGCAGGTTTTCGAGGACAGTGCTGCGGTCTCTCATCAGCCCAAGGTTGATGATCGAGCTTCGTCTTCGCCCATGTCGGTGCAAATGGCGTGGATTATGGAGCTTCTGGAGAGCAATTTGGAAGCCAAGTCGAAGATTTATGGGGACTCTGCGTTGTGCTGTGTTTTCATGATGAACAATGGGAGGTACATTGTGCAGAAAGTGAAGGACAGTGAGTTGGGATCGCTTTTGGGCGATGATTGGATCCGAAAACACACTGCGAAAATCCGACAGTACCTTGTGAATTACCAGAGAAGCTCATGGAACAGGGTGCTTGGAGTGTTGAAGCCCGAGAGTGGCTCATTGTCGCCAAGTGCTGCTGTGAAGTCCATGAAAGAGAAGCTCAAGTTGTTCAACATCTACTTCGAAGAGATCTGTAAAACGCAGTCCAATTGGGTAGTTTTTGACGATCAACTCAGAGATGATTTGAGAATTTCGCTTGTCAAAATCTTGTTGCCAGCGTATCAGAGCTTCATCGGGAGGTTTCAGAATGTTCCCGAAATCGGGCGGCATAAGTACATTAAGTATACTAGTGAGGACATTGATGCTAAGATCAATGGCTTGTTCCGCGGCAGCAGAGGATCCGCCGGCACTGGTGGTGGCCGGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000145 GO:0001101 GO:0002237 GO:0002238 GO:0002831 GO:0002833 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005938 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010035 GO:0010119 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016192 GO:0023051 GO:0023056 GO:0031347 GO:0031349 GO:0031982 GO:0032101 GO:0032103 GO:0032940 GO:0032991 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043230 GO:0043231 GO:0043621 GO:0043900 GO:0043902 GO:0044421 GO:0044424 GO:0044444 GO:0044448 GO:0044464 GO:0045229 GO:0046903 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0065007 GO:0070062 GO:0071554 GO:0071555 GO:0071840 GO:0071944 GO:0080134 GO:0090333 GO:0098542 GO:0099023 GO:0099568 GO:1900150 GO:1900424 GO:1900426 GO:1901419 GO:1901421 GO:1901700 GO:1902288 GO:1902290 GO:1903561 GO:1905957 GO:1905959

KEGG Pathways

Metabolic & Signaling

ko04910 map04910

Pfam Domains

Protein Families

Exo70

Protein Analysis

636

Amino Acids

71.9

Weight (kDa)

5.26

Isoelectric Point (pI)

49.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exo70_N	PF20669	64 - 140	2.1e-09	Exocyst complex component Exo70 N-terminal
Exo70_C	PF03081	266 - 618	1.4e-123	Exo70 exocyst complex subunit C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003033)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58430
fragaria_vesca	FvH4_3g39790
malus_domestica	MD03G1056800.v1.1
prunus_persica	Prupe.6G048000_v2.0.a1
pyrus_communis	pycom03g04520 pycom11g04860
rosa_chinensis	RchiOBHm_Chr5g0004641 RchiOBHm_Chr5g0071681
rosa_laevigata	RLG00000002358 RLG00000008736 RLG00000018357 RLG00000026108 RLG00000029385 RLG00000036239
rosa_multiflora	Rmu_sc0002255.1_g000001
rosa_roxburghii	Rroxscaffold_1G00005050 Rroxscaffold_1G00009490 Rroxscaffold_4G00312970
rosa_rugosa	Rorug05G0399200 Rorug06G0105500
rosa_samantha	Rh1BG001700 Rh1DG215500 Rh2CG371300 Rh5AG470200 Rh5BG236300 Rh5BG489700 Rh5BG566000 Rh5CG513500 Rh5CG588000 Rh5DG500500 Rh6AG501300 Rh6BG021300 Rh6BG467900 Rh7AG147100 Rh7BG007900 Rh7CG151300
rosa_wichuraiana	Rw5G036600 Rw5G043690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	335
Acc36I	ACCTGC	2 cut(s)	108, 1252
AccB7I	CCANNNNNTGG	1 cut(s)	1181
AccBSI	CCGCTC	1 cut(s)	796
AccI	GTMKAC	2 cut(s)	850, 1823
AcoI	YGGCCR	4 cut(s)	78, 252, 321, 1897
AcsI	RAATTY	2 cut(s)	1034, 1728
AcuI	CTGAAG	1 cut(s)	209
AcvI	CACGTG	1 cut(s)	676
AcyI	GRCGYC	1 cut(s)	764
AfaI	GTAC	5 cut(s)	680, 1061, 1445, 1532, 1814
AflIII	ACRYGT	2 cut(s)	675, 1002
AgsI	TTSAA	2 cut(s)	1582, 1651
AhdI	GACNNNNNGTC	1 cut(s)	331
AhlI	ACTAGT	1 cut(s)	1826
AjiI	CACGTC	1 cut(s)	1003
AjnI	CCWGG	2 cut(s)	702, 1180
AloI	GAACNNNNNNTCC	2 cut(s)	971, 1003
Alw21I	GWGCWC	3 cut(s)	183, 543, 671
Alw26I	GTCTC	3 cut(s)	489, 1004, 1291
AlwNI	CAGNNNCTG	4 cut(s)	687, 739, 937, 1181
Ama87I	CYCGRG	5 cut(s)	176, 392, 838, 1256, 1586
AoxI	GGCC	7 cut(s)	78, 243, 252, 321, 378, 874, 1897
ApoI	RAATTY	2 cut(s)	1034, 1728
AscI	GGCGCGCC	1 cut(s)	38
AsiSI	GCGATCGC	1 cut(s)	862
Asp700I	GAANNNNTTC	1 cut(s)	753
AspLEI	GCGC	9 cut(s)	40, 42, 170, 355, 452, 476, 595, 823, 1248
AspS9I	GGNCC	3 cut(s)	199, 243, 775
AsuC2I	CCSGG	1 cut(s)	917
AsuHPI	GGTGA	1 cut(s)	545
AsuII	TTCGAA	1 cut(s)	1662
AvaI	CYCGRG	5 cut(s)	176, 392, 838, 1256, 1586
AvaII	GGWCC	2 cut(s)	199, 775
BamHI	GGATCC	2 cut(s)	1500, 1877
BanII	GRGCYC	2 cut(s)	183, 923
BarI	GAAGNNNNNNTAC	2 cut(s)	1751, 1783
BbrPI	CACGTG	1 cut(s)	676
BbsI	GAAGAC	2 cut(s)	767, 1314
Bbv12I	GWGCWC	3 cut(s)	183, 543, 671
BbvCI	CCTCAGC	1 cut(s)	360
BccI	CCATC	2 cut(s)	617, 634
BceAI	ACGGC	3 cut(s)	28, 130, 889
BcgI	CGANNNNNNTGC	4 cut(s)	1224, 1258, 1259, 1293
BciT130I	CCWGG	2 cut(s)	704, 1182
BclI	TGATCA	1 cut(s)	1149
BcnI	CCSGG	1 cut(s)	917
BcoDI	GTCTC	3 cut(s)	489, 1004, 1291
BcuI	ACTAGT	1 cut(s)	1826
BfaI	CTAG	2 cut(s)	725, 1827
BfuAI	ACCTGC	2 cut(s)	108, 1252
BglI	GCCNNNNNGGC	1 cut(s)	251
BglII	AGATCT	1 cut(s)	1668
BlpI	GCTNAGC	3 cut(s)	558, 633, 740
BmcAI	AGTACT	1 cut(s)	1061
Bme1390I	CCNGG	3 cut(s)	704, 917, 1182
Bme18I	GGWCC	2 cut(s)	199, 775
BmeRI	GACNNNNNGTC	1 cut(s)	331
BmeT110I	CYCGRG	5 cut(s)	176, 392, 838, 1256, 1586
BmgBI	CACGTC	1 cut(s)	1003
BmgT120I	GGNCC	3 cut(s)	199, 243, 775
BmiI	GGNNCC	3 cut(s)	607, 1502, 1879
BmrFI	CCNGG	3 cut(s)	704, 917, 1182
BmsI	GCATC	6 cut(s)	580, 753, 873, 1017, 1204, 1831
BpiI	GAAGAC	2 cut(s)	767, 1314
BplI	GAGNNNNNCTC	2 cut(s)	1580, 1612
BpmI	CTGGAG	2 cut(s)	1158, 1385
Bpu10I	CCTNAGC	1 cut(s)	360
Bpu1102I	GCTNAGC	3 cut(s)	558, 633, 740
Bpu14I	TTCGAA	1 cut(s)	1662
BpuEI	CTTGAG	2 cut(s)	800, 1625
BpuMI	CCSGG	1 cut(s)	917
Bsa29I	ATCGAT	1 cut(s)	588
BsaAI	YACGTR	1 cut(s)	676
BsaHI	GRCGYC	1 cut(s)	764
BsaI	GGTCTC	1 cut(s)	1291
BsaJI	CCNNGG	7 cut(s)	261, 318, 411, 767, 916, 1299, 1864
BsaXI	ACNNNNNCTCC	4 cut(s)	863, 893, 971, 1001
Bse118I	RCCGGY	1 cut(s)	1883
Bse1I	ACTGG	3 cut(s)	314, 1175, 1894
BseBI	CCWGG	2 cut(s)	704, 1182
BseCI	ATCGAT	1 cut(s)	588
BseDI	CCNNGG	7 cut(s)	261, 318, 411, 767, 916, 1299, 1864
BseGI	GGATG	3 cut(s)	520, 645, 1195
BseMII	CTCAG	5 cut(s)	351, 572, 731, 1087, 1726
BseNI	ACTGG	3 cut(s)	314, 1175, 1894
BsePI	GCGCGC	1 cut(s)	38
BseRI	GAGGAG	2 cut(s)	551, 871
BseX3I	CGGCCG	1 cut(s)	252
BsgI	GTGCAG	2 cut(s)	1251, 1472
Bsh1285I	CGRYCG	5 cut(s)	255, 567, 862, 974, 1244
BshFI	GGCC	7 cut(s)	80, 245, 254, 323, 380, 876, 1899
BshVI	ATCGAT	1 cut(s)	588
BsiEI	CGRYCG	5 cut(s)	255, 567, 862, 974, 1244
BsiHKAI	GWGCWC	3 cut(s)	183, 543, 671
BsiHKCI	CYCGRG	5 cut(s)	176, 392, 838, 1256, 1586
BsiSI	CCGG	6 cut(s)	388, 691, 916, 977, 1884, 1900
BslFI	GGGAC	3 cut(s)	320, 542, 1415
BsmAI	GTCTC	3 cut(s)	489, 1004, 1291
BsmBI	CGTCTC	1 cut(s)	489
BsmFI	GGGAC	3 cut(s)	320, 542, 1415
BsnI	GGCC	7 cut(s)	80, 245, 254, 323, 380, 876, 1899
Bso31I	GGTCTC	1 cut(s)	1291
BsoBI	CYCGRG	5 cut(s)	176, 392, 838, 1256, 1586
Bsp119I	TTCGAA	1 cut(s)	1662
Bsp1286I	GDGCHC	4 cut(s)	183, 543, 671, 923
Bsp1407I	TGTACA	1 cut(s)	678
Bsp1720I	GCTNAGC	3 cut(s)	558, 633, 740
Bsp19I	CCATGG	2 cut(s)	318, 767
Bsp68I	TCGCGA	2 cut(s)	129, 1158
BspANI	GGCC	7 cut(s)	80, 245, 254, 323, 380, 876, 1899
BspCNI	CTCAG	5 cut(s)	352, 571, 732, 1086, 1725
BspDI	ATCGAT	1 cut(s)	588
BspHI	TCATGA	1 cut(s)	1425
BspLI	GGNNCC	3 cut(s)	607, 1502, 1879
BspMI	ACCTGC	2 cut(s)	108, 1252
BspT104I	TTCGAA	1 cut(s)	1662
BspTNI	GGTCTC	1 cut(s)	1291
BsrBI	CCGCTC	1 cut(s)	796
BsrFI	RCCGGY	1 cut(s)	1883
BsrGI	TGTACA	1 cut(s)	678
BsrI	ACTGG	3 cut(s)	314, 1175, 1894
BssAI	RCCGGY	1 cut(s)	1883
BssECI	CCNNGG	7 cut(s)	261, 318, 411, 767, 916, 1299, 1864
BssHII	GCGCGC	1 cut(s)	38
BssNAI	GTATAC	1 cut(s)	1824
BssNI	GRCGYC	1 cut(s)	764
BssT1I	CCWWGG	4 cut(s)	318, 411, 767, 1299
Bst1107I	GTATAC	1 cut(s)	1824
Bst2UI	CCWGG	2 cut(s)	704, 1182
Bst4CI	ACNGT	6 cut(s)	203, 533, 1064, 1277, 1469, 1530
Bst6I	CTCTTC	5 cut(s)	12, 504, 702, 744, 1659
BstACI	GRCGYC	1 cut(s)	764
BstAUI	TGTACA	1 cut(s)	678
BstBAI	YACGTR	1 cut(s)	676
BstBI	TTCGAA	1 cut(s)	1662
BstC8I	GCNNGC	9 cut(s)	40, 357, 378, 671, 731, 845, 1237, 1755, 1885
BstDEI	CTNAG	8 cut(s)	6, 360, 558, 633, 740, 1073, 1712, 1845
BstDSI	CCRYGG	4 cut(s)	261, 318, 767, 1864
BstENI	CCTNNNNNAGG	1 cut(s)	1259
BstF5I	GGATG	3 cut(s)	520, 645, 1195
BstHHI	GCGC	9 cut(s)	40, 42, 170, 355, 452, 476, 595, 823, 1248
BstMAI	GTCTC	3 cut(s)	489, 1004, 1291
BstMCI	CGRYCG	5 cut(s)	255, 567, 862, 974, 1244
BstMWI	GCNNNNNNNGC	7 cut(s)	162, 193, 251, 260, 730, 1245, 1863
BstNI	CCWGG	2 cut(s)	704, 1182
BstNSI	RCATGY	2 cut(s)	718, 1239
BstSCI	CCNGG	3 cut(s)	702, 915, 1180
BstV2I	GAAGAC	2 cut(s)	767, 1314
BstX2I	RGATCY	7 cut(s)	207, 364, 407, 546, 1500, 1668, 1877
BstXI	CCANNNNNNTGG	1 cut(s)	58
BstYI	RGATCY	7 cut(s)	207, 364, 407, 546, 1500, 1668, 1877
BstZ17I	GTATAC	1 cut(s)	1824
BstZI	CGGCCG	1 cut(s)	252
Bsu15I	ATCGAT	1 cut(s)	588
BsuRI	GGCC	7 cut(s)	80, 245, 254, 323, 380, 876, 1899
BsuTUI	ATCGAT	1 cut(s)	588
BtgI	CCRYGG	4 cut(s)	261, 318, 767, 1864
BtgZI	GCGATG	2 cut(s)	277, 1506
BtrI	CACGTC	1 cut(s)	1003
BtsCI	GGATG	3 cut(s)	520, 645, 1195
BtsI	GCAGTG	1 cut(s)	1512
BtsIMutI	CAGTG	4 cut(s)	1282, 1474, 1512, 1887
BtuMI	TCGCGA	2 cut(s)	129, 1158
BveI	ACCTGC	2 cut(s)	108, 1252
Cac8I	GCNNGC	9 cut(s)	40, 357, 378, 671, 731, 845, 1237, 1755, 1885
CaiI	CAGNNNCTG	4 cut(s)	687, 739, 937, 1181
CciI	TCATGA	1 cut(s)	1425
CfoI	GCGC	9 cut(s)	40, 42, 170, 355, 452, 476, 595, 823, 1248
Cfr10I	RCCGGY	1 cut(s)	1883
Cfr13I	GGNCC	3 cut(s)	199, 243, 775
Cfr42I	CCGCGG	2 cut(s)	264, 1867
ClaI	ATCGAT	1 cut(s)	588
CseI	GACGC	2 cut(s)	772, 959
Csp6I	GTAC	5 cut(s)	679, 1060, 1444, 1531, 1813
CspCI	CAANNNNNGTGG	2 cut(s)	1328, 1363
CviQI	GTAC	5 cut(s)	679, 1060, 1444, 1531, 1813
DdeI	CTNAG	8 cut(s)	6, 360, 558, 633, 740, 1073, 1712, 1845
DrdI	GACNNNNNNGTC	1 cut(s)	335
DriI	GACNNNNNGTC	1 cut(s)	331
DseDI	GACNNNNNNGTC	1 cut(s)	335
EaeI	YGGCCR	4 cut(s)	78, 252, 321, 1897
EagI	CGGCCG	1 cut(s)	252
Eam1104I	CTCTTC	5 cut(s)	12, 504, 702, 744, 1659
Eam1105I	GACNNNNNGTC	1 cut(s)	331
EarI	CTCTTC	5 cut(s)	12, 504, 702, 744, 1659
EciI	GGCGGA	2 cut(s)	874, 1870
Ecl136II	GAGCTC	1 cut(s)	181
EclXI	CGGCCG	1 cut(s)	252
Eco130I	CCWWGG	4 cut(s)	318, 411, 767, 1299
Eco24I	GRGCYC	2 cut(s)	183, 923
Eco31I	GGTCTC	1 cut(s)	1291
Eco47I	GGWCC	2 cut(s)	199, 775
Eco52I	CGGCCG	1 cut(s)	252
Eco53kI	GAGCTC	1 cut(s)	181
Eco57I	CTGAAG	1 cut(s)	209
Eco72I	CACGTG	1 cut(s)	676
Eco88I	CYCGRG	5 cut(s)	176, 392, 838, 1256, 1586
EcoICRI	GAGCTC	1 cut(s)	181
EcoNI	CCTNNNNNAGG	1 cut(s)	1259
EcoO109I	RGGNCCY	1 cut(s)	775
EcoRI	GAATTC	1 cut(s)	1034
EcoRII	CCWGG	2 cut(s)	702, 1180
EcoT14I	CCWWGG	4 cut(s)	318, 411, 767, 1299
EcoT38I	GRGCYC	2 cut(s)	183, 923
ErhI	CCWWGG	4 cut(s)	318, 411, 767, 1299
Esp3I	CGTCTC	1 cut(s)	489
FaqI	GGGAC	3 cut(s)	320, 542, 1415
FauI	CCCGC	3 cut(s)	643, 1011, 1181
FbaI	TGATCA	1 cut(s)	1149
FblI	GTMKAC	2 cut(s)	850, 1823
FokI	GGATG	3 cut(s)	527, 652, 1182
FriOI	GRGCYC	2 cut(s)	183, 923
FspBI	CTAG	2 cut(s)	725, 1827
GlaI	GCGC	9 cut(s)	39, 41, 169, 354, 451, 475, 594, 822, 1247
GsuI	CTGGAG	2 cut(s)	1158, 1385
HaeIII	GGCC	7 cut(s)	80, 245, 254, 323, 380, 876, 1899
HapII	CCGG	6 cut(s)	388, 691, 916, 977, 1884, 1900
HgaI	GACGC	2 cut(s)	772, 959
HhaI	GCGC	9 cut(s)	40, 42, 170, 355, 452, 476, 595, 823, 1248
Hin1I	GRCGYC	1 cut(s)	764
Hin6I	GCGC	9 cut(s)	38, 40, 168, 353, 450, 474, 593, 821, 1246
HinP1I	GCGC	9 cut(s)	38, 40, 168, 353, 450, 474, 593, 821, 1246
HpaII	CCGG	6 cut(s)	388, 691, 916, 977, 1884, 1900
HphI	GGTGA	1 cut(s)	545
Hpy166II	GTNNAC	3 cut(s)	679, 851, 1824
Hpy8I	GTNNAC	3 cut(s)	679, 851, 1824
HpyAV	CCTTC	2 cut(s)	609, 1456
HpyCH4III	ACNGT	6 cut(s)	203, 533, 1064, 1277, 1469, 1530
HpyCH4IV	ACGT	3 cut(s)	675, 816, 1002
HpyCH4V	TGCA	7 cut(s)	94, 373, 733, 1195, 1232, 1339, 1453
HpyF10VI	GCNNNNNNNGC	7 cut(s)	162, 193, 251, 260, 730, 1245, 1863
HpyF3I	CTNAG	8 cut(s)	6, 360, 558, 633, 740, 1073, 1712, 1845
HpySE526I	ACGT	3 cut(s)	675, 816, 1002
Hsp92I	GRCGYC	1 cut(s)	764
HspAI	GCGC	9 cut(s)	38, 40, 168, 353, 450, 474, 593, 821, 1246
KroI	GCCGGC	1 cut(s)	1883
KroNI	GCCGGC	1 cut(s)	1885
Ksp22I	TGATCA	1 cut(s)	1149
KspI	CCGCGG	2 cut(s)	264, 1867
LmnI	GCTCC	3 cut(s)	538, 979, 1357
LweI	GCATC	6 cut(s)	580, 753, 873, 1017, 1204, 1831
MaeI	CTAG	2 cut(s)	725, 1827
MaeII	ACGT	3 cut(s)	675, 816, 1002
MaeIII	GTNAC	1 cut(s)	432
MbiI	CCGCTC	1 cut(s)	796
MfeI	CAATTG	1 cut(s)	1687
MflI	RGATCY	7 cut(s)	207, 364, 407, 546, 1500, 1668, 1877
MhlI	GDGCHC	4 cut(s)	183, 543, 671, 923
MluCI	AATT	9 cut(s)	23, 134, 522, 1034, 1219, 1372, 1540, 1687, 1728
MlyI	GAGTC	8 cut(s)	49, 307, 440, 473, 497, 846, 1006, 1397
MmeI	TCCRAC	5 cut(s)	129, 168, 185, 1119, 1549
MroNI	GCCGGC	1 cut(s)	1883
MroXI	GAANNNNTTC	1 cut(s)	753
MseI	TTAA	2 cut(s)	273, 1818
MslI	CAYNNNNRTG	2 cut(s)	1130, 1334
MspA1I	CMGCKG	4 cut(s)	165, 263, 687, 1866
MspI	CCGG	6 cut(s)	388, 691, 916, 977, 1884, 1900
MspR9I	CCNGG	3 cut(s)	704, 917, 1182
MunI	CAATTG	1 cut(s)	1687
MvaI	CCWGG	2 cut(s)	704, 1182
MwoI	GCNNNNNNNGC	7 cut(s)	162, 193, 251, 260, 730, 1245, 1863
NaeI	GCCGGC	1 cut(s)	1885
NciI	CCSGG	1 cut(s)	917
NcoI	CCATGG	2 cut(s)	318, 767
NgoMIV	GCCGGC	1 cut(s)	1883
NlaIV	GGNNCC	3 cut(s)	607, 1502, 1879
NmeAIII	GCCGAG	1 cut(s)	230
NmuCI	GTSAC	1 cut(s)	432
NruI	TCGCGA	2 cut(s)	129, 1158
NspI	RCATGY	2 cut(s)	718, 1239
NspV	TTCGAA	1 cut(s)	1662
PaeI	GCATGC	1 cut(s)	1239
PaeR7I	CTCGAG	3 cut(s)	176, 392, 1256
PagI	TCATGA	1 cut(s)	1425
PalAI	GGCGCGCC	1 cut(s)	38
PauI	GCGCGC	1 cut(s)	38
PcsI	WCGNNNNNNNCGW	3 cut(s)	333, 573, 1005
PdiI	GCCGGC	1 cut(s)	1885
PdmI	GAANNNNTTC	1 cut(s)	753
PfeI	GAWTC	5 cut(s)	257, 829, 928, 994, 1144
PflMI	CCANNNNNTGG	1 cut(s)	1181
PfoI	TCCNGGA	1 cut(s)	702
Ple19I	CGATCG	4 cut(s)	567, 862, 974, 1244
PleI	GAGTC	8 cut(s)	49, 307, 439, 472, 496, 846, 1006, 1397
PmaCI	CACGTG	1 cut(s)	676
PmlI	CACGTG	1 cut(s)	676
PpsI	GAGTC	8 cut(s)	49, 307, 439, 472, 496, 846, 1006, 1397
Ppu21I	YACGTR	1 cut(s)	676
PpuMI	RGGWCCY	1 cut(s)	775
Psp124BI	GAGCTC	1 cut(s)	183
Psp5II	RGGWCCY	1 cut(s)	775
Psp6I	CCWGG	2 cut(s)	702, 1180
PspCI	CACGTG	1 cut(s)	676
PspGI	CCWGG	2 cut(s)	702, 1180
PspN4I	GGNNCC	3 cut(s)	607, 1502, 1879
PspPI	GGNCC	3 cut(s)	199, 243, 775
PspPPI	RGGWCCY	1 cut(s)	775
PspXI	VCTCGAGB	3 cut(s)	176, 392, 1256
PstNI	CAGNNNCTG	4 cut(s)	687, 739, 937, 1181
PsuI	RGATCY	7 cut(s)	207, 364, 407, 546, 1500, 1668, 1877
PteI	GCGCGC	1 cut(s)	38
PvuI	CGATCG	4 cut(s)	567, 862, 974, 1244
PvuII	CAGCTG	2 cut(s)	165, 687
RgaI	GCGATCGC	1 cut(s)	862
RruI	TCGCGA	2 cut(s)	129, 1158
RsaI	GTAC	5 cut(s)	680, 1061, 1445, 1532, 1814
RsaNI	GTAC	5 cut(s)	679, 1060, 1444, 1531, 1813
RseI	CAYNNNNRTG	2 cut(s)	1130, 1334
SacI	GAGCTC	1 cut(s)	183
SacII	CCGCGG	2 cut(s)	264, 1867
SaqAI	TTAA	2 cut(s)	273, 1818
Sau96I	GGNCC	3 cut(s)	199, 243, 775
ScaI	AGTACT	1 cut(s)	1061
SchI	GAGTC	8 cut(s)	49, 307, 440, 473, 497, 846, 1006, 1397
ScrFI	CCNGG	3 cut(s)	704, 917, 1182
SduI	GDGCHC	4 cut(s)	183, 543, 671, 923
SfaAI	GCGATCGC	1 cut(s)	862
SfaNI	GCATC	6 cut(s)	580, 753, 873, 1017, 1204, 1831
SfiI	GGCCNNNNNGGCC	1 cut(s)	251
Sfr274I	CTCGAG	3 cut(s)	176, 392, 1256
Sfr303I	CCGCGG	2 cut(s)	264, 1867
SfuI	TTCGAA	1 cut(s)	1662
SgfI	GCGATCGC	1 cut(s)	862
SgrBI	CCGCGG	2 cut(s)	264, 1867
SgsI	GGCGCGCC	1 cut(s)	38
SinI	GGWCC	2 cut(s)	199, 775
SlaI	CTCGAG	3 cut(s)	176, 392, 1256
SmiMI	CAYNNNNRTG	2 cut(s)	1130, 1334
SmlI	CTYRAG	5 cut(s)	176, 392, 779, 1256, 1640
SmoI	CTYRAG	5 cut(s)	176, 392, 779, 1256, 1640
SpeI	ACTAGT	1 cut(s)	1826
SphI	GCATGC	1 cut(s)	1239
Sse9I	AATT	9 cut(s)	23, 134, 522, 1034, 1219, 1372, 1540, 1687, 1728
SspMI	CTAG	2 cut(s)	725, 1827
SstI	GAGCTC	1 cut(s)	183
StyD4I	CCNGG	3 cut(s)	702, 915, 1180
StyI	CCWWGG	4 cut(s)	318, 411, 767, 1299
TaaI	ACNGT	6 cut(s)	203, 533, 1064, 1277, 1469, 1530
TaiI	ACGT	3 cut(s)	678, 819, 1005
TasI	AATT	9 cut(s)	23, 134, 522, 1034, 1219, 1372, 1540, 1687, 1728
TatI	WGTACW	3 cut(s)	678, 1059, 1812
TauI	GCSGC	9 cut(s)	173, 266, 426, 429, 650, 890, 1188, 1807, 1869
TfiI	GAWTC	5 cut(s)	257, 829, 928, 994, 1144
Tru1I	TTAA	2 cut(s)	273, 1818
Tru9I	TTAA	2 cut(s)	273, 1818
TscAI	CASTG	4 cut(s)	1282, 1474, 1519, 1894
TseFI	GTSAC	1 cut(s)	432
Tsp45I	GTSAC	1 cut(s)	432
TspDTI	ATGAA	9 cut(s)	446, 516, 892, 1120, 1232, 1414, 1445, 1643, 1759
TspGWI	ACGGA	2 cut(s)	325, 1217
TspRI	CASTG	4 cut(s)	1282, 1474, 1519, 1894
Van91I	CCANNNNNTGG	1 cut(s)	1181
VpaK11BI	GGWCC	2 cut(s)	199, 775
XagI	CCTNNNNNAGG	1 cut(s)	1259
XapI	RAATTY	2 cut(s)	1034, 1728
XceI	RCATGY	2 cut(s)	718, 1239
XhoI	CTCGAG	3 cut(s)	176, 392, 1256
XmiI	GTMKAC	2 cut(s)	850, 1823
XmnI	GAANNNNTTC	1 cut(s)	753
XspI	CTAG	2 cut(s)	725, 1827
ZrmI	AGTACT	1 cut(s)	1061

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.