MD03G1099000.v1.1

Belongs to the universal ribosomal protein uS8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005993
Physical Location & Seq
Forward (+)
8469123 .. 8471066
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1099000.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGTGCGCGTCAGCGTTCTCAATGACGCTCTGAAGAGCATGTACAACGCCGAGAAGAGGGGAAAGCGACAGGTCATGATCCGGCCGTCGTCAAAGGTCATCATCAAGTTCCTCCTGGTTATGCAGAAGCATGGATACATTGGGGAATTTGAGTATGTTGATGACCATAGAGCTGGAAAGATTGTGGTTGAGCTCAATGGGAGGCTGAACAAGTGTGGGGTTATCAGTCCCCGATTCGATGTCGGTGTGAAGGAGATTGAAGGCTGGACTGCTAGGCTTCTGCCTTCCAGACAGTTCGGATATATTGTGCTGACCACATCTGCTGGAATCATGGACCATGAAGAAGCTAGGAGAAAGAATGTTGGTGGCAAGGTCCTCGGGGAGTCTAGCAGAGGAAATGAAGTATGCCATTGTAGGTTCTCGTGCTATTTCAAAGAGGTCTTAAGCCTTGAGAAATGCCGAGGCAGATGCATAAGACCCTGGAGTAGGCACGGGCAATGGATACCCATGAGGTTTTGGGATGTGGTGCTGGTGCTGGTGGGTACCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S8

Protein Analysis

184

Amino Acids

20.99

Weight (kDa)

9.78

Isoelectric Point (pI)

28.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8 PF00410 7 - 127 1.5e-20 Ribosomal protein S8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 542
AccB1I GGYRCC 1 cut(s) 542
AccII CGCG 1 cut(s) 9
AclWI GGATC 1 cut(s) 73
AcoI YGGCCR 1 cut(s) 83
AcsI RAATTY 1 cut(s) 146
AcuI CTGAAG 1 cut(s) 53
AfaI GTAC 2 cut(s) 44, 544
AfiI CCNNNNNNNGG 2 cut(s) 57, 486
AflII CTTAAG 1 cut(s) 442
AgsI TTSAA 2 cut(s) 260, 433
AjnI CCWGG 2 cut(s) 114, 479
AluBI AGCT 3 cut(s) 173, 193, 347
AluI AGCT 3 cut(s) 173, 193, 347
Alw21I GWGCWC 1 cut(s) 195
AlwI GGATC 1 cut(s) 73
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 1 cut(s) 83
ApoI RAATTY 1 cut(s) 146
Asp718I GGTACC 1 cut(s) 542
AspLEI GCGC 1 cut(s) 9
AspS9I GGNCC 2 cut(s) 334, 373
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 2 cut(s) 334, 373
BanI GGYRCC 1 cut(s) 542
BanII GRGCYC 1 cut(s) 195
BarI GAAGNNNNNNTAC 4 cut(s) 26, 58, 119, 151
BauI CACGAG 1 cut(s) 421
Bbv12I GWGCWC 1 cut(s) 195
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 4 cut(s) 358, 392, 450, 484
BciT130I CCWGG 2 cut(s) 116, 481
BciVI GTATCC 2 cut(s) 128, 495
BfaI CTAG 3 cut(s) 273, 348, 387
BfrI CTTAAG 1 cut(s) 442
BfuI GTATCC 2 cut(s) 128, 495
Bme1390I CCNGG 2 cut(s) 116, 481
Bme18I GGWCC 2 cut(s) 334, 373
BmeT110I CYCGRG 1 cut(s) 377
BmgT120I GGNCC 2 cut(s) 334, 373
BmiI GGNNCC 1 cut(s) 544
BmrFI CCNGG 2 cut(s) 116, 481
BmsI GCATC 1 cut(s) 458
BpmI CTGGAG 1 cut(s) 502
BpuEI CTTGAG 1 cut(s) 470
BsaJI CCNNGG 3 cut(s) 376, 460, 479
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 486
Bse3DI GCAATG 1 cut(s) 503
BseBI CCWGG 2 cut(s) 116, 481
BseDI CCNNGG 3 cut(s) 376, 460, 479
BseGI GGATG 1 cut(s) 526
BseLI CCNNNNNNNGG 2 cut(s) 57, 486
BseMI GCAATG 1 cut(s) 503
BseX3I CGGCCG 1 cut(s) 83
Bsh1236I CGCG 1 cut(s) 9
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 1 cut(s) 85
BshNI GGYRCC 1 cut(s) 542
BsiEI CGRYCG 1 cut(s) 86
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 1 cut(s) 82
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 2 cut(s) 57, 486
BsmFI GGGAC 1 cut(s) 213
BsnI GGCC 1 cut(s) 85
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 195
Bsp1407I TGTACA 1 cut(s) 42
Bsp143I GATC 1 cut(s) 78
BspANI GGCC 1 cut(s) 85
BspFNI CGCG 1 cut(s) 9
BspHI TCATGA 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 544
BspPI GGATC 1 cut(s) 73
BspQI GCTCTTC 1 cut(s) 29
BspT107I GGYRCC 1 cut(s) 542
BspTI CTTAAG 1 cut(s) 442
BsrDI GCAATG 1 cut(s) 503
BsrGI TGTACA 1 cut(s) 42
BssECI CCNNGG 3 cut(s) 376, 460, 479
BssMI GATC 1 cut(s) 78
BssSI CACGAG 1 cut(s) 421
Bst2BI CACGAG 1 cut(s) 421
Bst2UI CCWGG 2 cut(s) 116, 481
Bst4CI ACNGT 1 cut(s) 294
Bst6I CTCTTC 2 cut(s) 29, 50
BstAFI CTTAAG 1 cut(s) 442
BstAUI TGTACA 1 cut(s) 42
BstENI CCTNNNNNAGG 1 cut(s) 484
BstF5I GGATG 1 cut(s) 526
BstFNI CGCG 1 cut(s) 9
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 1 cut(s) 81
BstMBI GATC 1 cut(s) 78
BstMCI CGRYCG 1 cut(s) 86
BstNI CCWGG 2 cut(s) 116, 481
BstNSI RCATGY 1 cut(s) 43
BstSCI CCNGG 2 cut(s) 114, 479
BstUI CGCG 1 cut(s) 9
BstXI CCANNNNNNTGG 1 cut(s) 173
BstZI CGGCCG 1 cut(s) 83
BsuI GTATCC 2 cut(s) 128, 495
BsuRI GGCC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 526
CciI TCATGA 1 cut(s) 75
CfoI GCGC 1 cut(s) 9
Cfr13I GGNCC 2 cut(s) 334, 373
CseI GACGC 1 cut(s) 35
Csp6I GTAC 2 cut(s) 43, 543
CviAII CATG 6 cut(s) 40, 76, 131, 331, 338, 508
CviJI RGCY 8 cut(s) 85, 173, 193, 205, 264, 277, 347, 447
CviKI_1 RGCY 8 cut(s) 85, 173, 193, 205, 264, 277, 347, 447
CviQI GTAC 2 cut(s) 43, 543
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
EaeI YGGCCR 1 cut(s) 83
EagI CGGCCG 1 cut(s) 83
Eam1104I CTCTTC 2 cut(s) 29, 50
EarI CTCTTC 2 cut(s) 29, 50
Ecl136II GAGCTC 1 cut(s) 193
EclXI CGGCCG 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 2 cut(s) 334, 373
Eco52I CGGCCG 1 cut(s) 83
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 53
Eco88I CYCGRG 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 193
EcoNI CCTNNNNNAGG 1 cut(s) 484
EcoO109I RGGNCCY 1 cut(s) 373
EcoRII CCWGG 2 cut(s) 114, 479
EcoT22I ATGCAT 1 cut(s) 473
EcoT38I GRGCYC 1 cut(s) 195
FaeI CATG 6 cut(s) 43, 79, 134, 334, 341, 511
FaqI GGGAC 1 cut(s) 213
FatI CATG 6 cut(s) 39, 75, 130, 330, 337, 507
FokI GGATG 1 cut(s) 533
FriOI GRGCYC 1 cut(s) 195
FspBI CTAG 3 cut(s) 273, 348, 387
GlaI GCGC 1 cut(s) 8
GsuI CTGGAG 1 cut(s) 502
HaeIII GGCC 1 cut(s) 85
HapII CCGG 1 cut(s) 82
HgaI GACGC 1 cut(s) 35
HhaI GCGC 1 cut(s) 9
Hin1II CATG 6 cut(s) 43, 79, 134, 334, 341, 511
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HinfI GANTC 3 cut(s) 234, 327, 383
HpaII CCGG 1 cut(s) 82
Hpy188I TCNGA 2 cut(s) 33, 299
Hpy188III TCNNGA 2 cut(s) 76, 288
Hpy99I CGWCG 1 cut(s) 91
HpyAV CCTTC 3 cut(s) 244, 254, 294
HpyCH4III ACNGT 1 cut(s) 294
HpyCH4V TGCA 2 cut(s) 124, 471
Hsp92II CATG 6 cut(s) 43, 79, 134, 334, 341, 511
HspAI GCGC 1 cut(s) 7
KpnI GGTACC 1 cut(s) 546
Kzo9I GATC 1 cut(s) 78
LguI GCTCTTC 1 cut(s) 29
LweI GCATC 1 cut(s) 458
MaeI CTAG 3 cut(s) 273, 348, 387
MalI GATC 1 cut(s) 80
MboI GATC 1 cut(s) 78
MboII GAAGA 3 cut(s) 46, 67, 353
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 1 cut(s) 146
MlyI GAGTC 1 cut(s) 392
MnlI CCTC 8 cut(s) 51, 122, 195, 386, 386, 430, 455, 504
Mph1103I ATGCAT 1 cut(s) 473
MseI TTAA 1 cut(s) 443
MspCI CTTAAG 1 cut(s) 442
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 2 cut(s) 116, 481
MvaI CCWGG 2 cut(s) 116, 481
MvnI CGCG 1 cut(s) 9
NdeII GATC 1 cut(s) 78
NlaIII CATG 6 cut(s) 43, 79, 134, 334, 341, 511
NlaIV GGNNCC 1 cut(s) 544
NmeAIII GCCGAG 2 cut(s) 76, 485
NsiI ATGCAT 1 cut(s) 473
NspI RCATGY 1 cut(s) 43
PagI TCATGA 1 cut(s) 75
PciSI GCTCTTC 1 cut(s) 29
PfeI GAWTC 2 cut(s) 234, 327
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
PpuMI RGGWCCY 1 cut(s) 373
Psp124BI GAGCTC 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 114, 479
PspGI CCWGG 2 cut(s) 114, 479
PspN4I GGNNCC 1 cut(s) 544
PspPI GGNCC 2 cut(s) 334, 373
PspPPI RGGWCCY 1 cut(s) 373
RsaI GTAC 2 cut(s) 44, 544
RsaNI GTAC 2 cut(s) 43, 543
SacI GAGCTC 1 cut(s) 195
SapI GCTCTTC 1 cut(s) 29
SaqAI TTAA 1 cut(s) 443
Sau3AI GATC 1 cut(s) 78
Sau96I GGNCC 2 cut(s) 334, 373
SchI GAGTC 1 cut(s) 392
ScrFI CCNGG 2 cut(s) 116, 481
SduI GDGCHC 1 cut(s) 195
SetI ASST 9 cut(s) 75, 99, 175, 195, 349, 375, 419, 441, 515
SfaNI GCATC 1 cut(s) 458
SinI GGWCC 2 cut(s) 334, 373
SmlI CTYRAG 2 cut(s) 442, 449
SmoI CTYRAG 2 cut(s) 442, 449
Sse9I AATT 1 cut(s) 146
SspMI CTAG 3 cut(s) 273, 348, 387
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 114, 479
TaaI ACNGT 1 cut(s) 294
TaqI TCGA 1 cut(s) 237
TasI AATT 1 cut(s) 146
TatI WGTACW 1 cut(s) 42
TfiI GAWTC 2 cut(s) 234, 327
Tru1I TTAA 1 cut(s) 443
Tru9I TTAA 1 cut(s) 443
TspDTI ATGAA 2 cut(s) 354, 414
Vha464I CTTAAG 1 cut(s) 442
VpaK11BI GGWCC 2 cut(s) 334, 373
XagI CCTNNNNNAGG 1 cut(s) 484
XapI RAATTY 1 cut(s) 146
XceI RCATGY 1 cut(s) 43
XspI CTAG 3 cut(s) 273, 348, 387
Zsp2I ATGCAT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.