MD03G1127500.v1.1

Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004109
Physical Location & Seq
Forward (+)
12380842 .. 12382816
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1127500.v1.1.491

Sequence Viewer

Length: 876 bp
ATGGATGCGCTTGGAAACCATCTAGAGTGTCATAACATCGACAGCTCTGAGTTACATGATTTCATAGATGATGCAAACCTTGATCAATTCATTGATCTTATTCGCGGTGGCGAAGGTGAAGATCCGATAGCTAATTTTGATTGTGAACTTATGATCAATGGCTGCTTGGATGATTATAACCTGTTCGGTCCAGCTAGTACTACTCCTCCAAGTCCTATGTTTGGTTTCAATGATGCATTTCTACCTGACCCGAGCACTTTTGTGTCAGAAACAACAAACACAAACACAACCACGACGACAACAAAGAGGCAAAAGGTTGATCGGTCACGAACTTTGGTGTCAGAGAGGAAGAGGAGGGGGAGAATGAAGGAAAGGCTCTATGCATTGCGGTCCTTGGTTCCTAACATAACTAAGATGGACAAGGCCTCCATTGTTCGAGATGCTGTATTGTATGTACAAGACTTGCAAATGCATGCTAAGAAGTTAAATGCTGAGATTGCAAACCTTGAAGCATCATTAGCAGGAGGATATCTACAAGGATCAACCAAAACTAAAAACAAGAAAAAAGTTTCAGACAATAACCATTTAGCTTCTAAAGGCATCATTCAGATAGACGTGTCTCAAGTGGAGGAAAAAGGTTTTTATGTGAAGGTGACATGCAACAAGGGACAAGTAGTAGCTACAGCACTTTACAAGGCTCTCGAATCTCTTGCACGCTTCAATGTTCAGAGTTCTAACTTAAACACGGTTTCAGCAGACAGATTTGAATTCGCATTCACTTTGAATGCAAAGGATTGCGAGCAGGGCATCATCAACTTGCCAAATTTGAAACATTGGGTGGCTGGGGCTTTTCTGAACCAAGGCTTTGTAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

292

Amino Acids

32.3

Weight (kDa)

6.1

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 112 - 156 2.2e-10 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AccII CGCG 1 cut(s) 105
AciI CCGC 2 cut(s) 105, 388
AclWI GGATC 2 cut(s) 116, 547
AcsI RAATTY 2 cut(s) 767, 823
AfaI GTAC 2 cut(s) 199, 456
AfiI CCNNNNNNNGG 1 cut(s) 221
AflIII ACRYGT 1 cut(s) 615
AgsI TTSAA 6 cut(s) 229, 509, 721, 767, 784, 829
AjiI CACGTC 1 cut(s) 616
AleI CACNNNNGTG 1 cut(s) 260
AluBI AGCT 5 cut(s) 45, 131, 194, 590, 680
AluI AGCT 5 cut(s) 45, 131, 194, 590, 680
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 1 cut(s) 624
AlwI GGATC 2 cut(s) 116, 547
Ama87I CYCGRG 1 cut(s) 250
AoxI GGCC 1 cut(s) 423
ApeKI GCWGC 1 cut(s) 162
ApoI RAATTY 2 cut(s) 767, 823
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 2 cut(s) 188, 390
AsuHPI GGTGA 2 cut(s) 128, 664
AvaI CYCGRG 1 cut(s) 250
AvaII GGWCC 2 cut(s) 188, 390
Bbv12I GWGCWC 1 cut(s) 257
BbvI GCAGC 1 cut(s) 149
BccI CCATC 2 cut(s) 27, 409
BcgI CGANNNNNNTGC 2 cut(s) 692, 726
BclI TGATCA 2 cut(s) 82, 153
BcoDI GTCTC 1 cut(s) 624
BfaI CTAG 2 cut(s) 23, 195
BfmI CTRYAG 1 cut(s) 681
BisI GCNGC 1 cut(s) 163
BlsI GCNGC 1 cut(s) 164
BmcAI AGTACT 1 cut(s) 199
Bme18I GGWCC 2 cut(s) 188, 390
BmeT110I CYCGRG 1 cut(s) 250
BmgBI CACGTC 1 cut(s) 616
BmgT120I GGNCC 2 cut(s) 188, 390
BmiI GGNNCC 1 cut(s) 399
BmsI GCATC 6 cut(s) 61, 223, 430, 521, 609, 816
BpuEI CTTGAG 1 cut(s) 606
BsaJI CCNNGG 2 cut(s) 393, 859
BsaXI ACNNNNNCTCC 4 cut(s) 190, 220, 410, 440
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse3DI GCAATG 1 cut(s) 383
BseDI CCNNGG 2 cut(s) 393, 859
BseGI GGATG 2 cut(s) 10, 175
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMI GCAATG 1 cut(s) 383
BseMII CTCAG 2 cut(s) 39, 483
BseRI GAGGAG 2 cut(s) 195, 367
BseXI GCAGC 1 cut(s) 149
BseYI CCCAGC 1 cut(s) 842
Bsh1236I CGCG 1 cut(s) 105
BshFI GGCC 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 257
BsiHKCI CYCGRG 1 cut(s) 250
BslFI GGGAC 1 cut(s) 681
BslI CCNNNNNNNGG 1 cut(s) 221
BsmAI GTCTC 1 cut(s) 624
BsmFI GGGAC 1 cut(s) 681
BsmI GAATGC 2 cut(s) 773, 790
BsnI GGCC 1 cut(s) 425
BsoBI CYCGRG 1 cut(s) 250
Bsp1286I GDGCHC 1 cut(s) 257
Bsp1407I TGTACA 1 cut(s) 454
Bsp143I GATC 6 cut(s) 82, 94, 121, 153, 319, 539
BspACI CCGC 2 cut(s) 105, 388
BspANI GGCC 1 cut(s) 425
BspCNI CTCAG 2 cut(s) 40, 484
BspFNI CGCG 1 cut(s) 105
BspLI GGNNCC 1 cut(s) 399
BspPI GGATC 2 cut(s) 116, 547
BsrDI GCAATG 1 cut(s) 383
BsrGI TGTACA 1 cut(s) 454
BssECI CCNNGG 2 cut(s) 393, 859
BssMI GATC 6 cut(s) 82, 94, 121, 153, 319, 539
BssT1I CCWWGG 2 cut(s) 393, 859
Bst4CI ACNGT 1 cut(s) 748
Bst6I CTCTTC 1 cut(s) 344
BstAUI TGTACA 1 cut(s) 454
BstC8I GCNNGC 3 cut(s) 474, 715, 800
BstDEI CTNAG 4 cut(s) 48, 411, 477, 492
BstF5I GGATG 2 cut(s) 10, 175
BstFNI CGCG 1 cut(s) 105
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 6 cut(s) 85, 97, 124, 156, 322, 542
BstMAI GTCTC 1 cut(s) 624
BstMBI GATC 6 cut(s) 82, 94, 121, 153, 319, 539
BstMWI GCNNNNNNNGC 3 cut(s) 497, 518, 804
BstNSI RCATGY 2 cut(s) 476, 660
BstSFI CTRYAG 1 cut(s) 681
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 1 cut(s) 149
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BsuRI GGCC 1 cut(s) 425
BtrI CACGTC 1 cut(s) 616
BtsCI GGATG 2 cut(s) 10, 175
Cac8I GCNNGC 3 cut(s) 474, 715, 800
CfoI GCGC 1 cut(s) 10
Cfr13I GGNCC 2 cut(s) 188, 390
Csp6I GTAC 2 cut(s) 198, 455
CviAII CATG 3 cut(s) 56, 473, 657
CviQI GTAC 2 cut(s) 198, 455
DdeI CTNAG 4 cut(s) 48, 411, 477, 492
DpnI GATC 6 cut(s) 84, 96, 123, 155, 321, 541
DpnII GATC 6 cut(s) 82, 94, 121, 153, 319, 539
Eam1104I CTCTTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 344
Eco130I CCWWGG 2 cut(s) 393, 859
Eco147I AGGCCT 1 cut(s) 425
Eco32I GATATC 1 cut(s) 530
Eco47I GGWCC 2 cut(s) 188, 390
Eco88I CYCGRG 1 cut(s) 250
EcoRI GAATTC 1 cut(s) 767
EcoRV GATATC 1 cut(s) 530
EcoT14I CCWWGG 2 cut(s) 393, 859
EcoT22I ATGCAT 3 cut(s) 238, 385, 474
ErhI CCWWGG 2 cut(s) 393, 859
FaeI CATG 3 cut(s) 59, 476, 660
FaqI GGGAC 1 cut(s) 681
FatI CATG 3 cut(s) 55, 472, 656
FbaI TGATCA 2 cut(s) 82, 153
Fnu4HI GCNGC 1 cut(s) 163
FokI GGATG 2 cut(s) 17, 182
Fsp4HI GCNGC 1 cut(s) 163
FspBI CTAG 2 cut(s) 23, 195
GlaI GCGC 1 cut(s) 9
GluI GCNGC 1 cut(s) 163
GsaI CCCAGC 1 cut(s) 846
HaeIII GGCC 1 cut(s) 425
HhaI GCGC 1 cut(s) 10
Hin1II CATG 3 cut(s) 59, 476, 660
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HinfI GANTC 1 cut(s) 704
HphI GGTGA 2 cut(s) 128, 664
Hpy166II GTNNAC 1 cut(s) 146
Hpy188I TCNGA 9 cut(s) 49, 126, 268, 343, 574, 609, 729, 855, 875
Hpy188III TCNNGA 4 cut(s) 23, 327, 437, 701
Hpy8I GTNNAC 1 cut(s) 146
Hpy99I CGWCG 1 cut(s) 298
HpyAV CCTTC 3 cut(s) 107, 361, 643
HpyCH4III ACNGT 1 cut(s) 748
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 9 cut(s) 74, 236, 383, 466, 472, 500, 660, 713, 788
HpyF10VI GCNNNNNNNGC 3 cut(s) 497, 518, 804
HpyF3I CTNAG 4 cut(s) 48, 411, 477, 492
HpySE526I ACGT 1 cut(s) 615
Hsp92II CATG 3 cut(s) 59, 476, 660
HspAI GCGC 1 cut(s) 8
Ksp22I TGATCA 2 cut(s) 82, 153
Kzo9I GATC 6 cut(s) 82, 94, 121, 153, 319, 539
LpnPI CCDG 6 cut(s) 194, 204, 258, 507, 788, 828
Lsp1109I GCAGC 1 cut(s) 149
LweI GCATC 6 cut(s) 61, 223, 430, 521, 609, 816
MaeI CTAG 2 cut(s) 23, 195
MaeII ACGT 1 cut(s) 615
MaeIII GTNAC 3 cut(s) 51, 324, 652
MalI GATC 6 cut(s) 84, 96, 123, 155, 321, 541
MboI GATC 6 cut(s) 82, 94, 121, 153, 319, 539
MboII GAAGA 2 cut(s) 131, 361
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 257
MluCI AATT 4 cut(s) 86, 133, 767, 823
MnlI CCTC 8 cut(s) 216, 300, 339, 345, 348, 436, 518, 622
Mph1103I ATGCAT 3 cut(s) 238, 385, 474
MseI TTAA 2 cut(s) 485, 740
MslI CAYNNNNRTG 1 cut(s) 260
Mva1269I GAATGC 2 cut(s) 773, 790
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 3 cut(s) 497, 518, 804
NdeII GATC 6 cut(s) 82, 94, 121, 153, 319, 539
NlaIII CATG 3 cut(s) 59, 476, 660
NlaIV GGNNCC 1 cut(s) 399
NmuCI GTSAC 2 cut(s) 324, 652
NsiI ATGCAT 3 cut(s) 238, 385, 474
NspI RCATGY 2 cut(s) 476, 660
OliI CACNNNNGTG 1 cut(s) 260
PaeI GCATGC 1 cut(s) 476
PceI AGGCCT 1 cut(s) 425
PctI GAATGC 2 cut(s) 773, 790
PfeI GAWTC 1 cut(s) 704
PkrI GCNGC 1 cut(s) 164
PsiI TTATAA 1 cut(s) 177
PspFI CCCAGC 1 cut(s) 842
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 2 cut(s) 188, 390
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 2 cut(s) 199, 456
RsaNI GTAC 2 cut(s) 198, 455
RseI CAYNNNNRTG 1 cut(s) 260
SaqAI TTAA 2 cut(s) 485, 740
SatI GCNGC 1 cut(s) 163
Sau3AI GATC 6 cut(s) 82, 94, 121, 153, 319, 539
Sau96I GGNCC 2 cut(s) 188, 390
ScaI AGTACT 1 cut(s) 199
SduI GDGCHC 1 cut(s) 257
SfaNI GCATC 6 cut(s) 61, 223, 430, 521, 609, 816
SfcI CTRYAG 1 cut(s) 681
SinI GGWCC 2 cut(s) 188, 390
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 621
SmoI CTYRAG 1 cut(s) 621
SphI GCATGC 1 cut(s) 476
Sse9I AATT 4 cut(s) 86, 133, 767, 823
SseBI AGGCCT 1 cut(s) 425
SsiI CCGC 2 cut(s) 105, 388
SspMI CTAG 2 cut(s) 23, 195
StuI AGGCCT 1 cut(s) 425
StyI CCWWGG 2 cut(s) 393, 859
TaaI ACNGT 1 cut(s) 748
TaiI ACGT 1 cut(s) 618
TaqI TCGA 3 cut(s) 39, 436, 702
TaqII GACCGA 2 cut(s) 176, 312
TasI AATT 4 cut(s) 86, 133, 767, 823
TatI WGTACW 2 cut(s) 197, 454
TfiI GAWTC 1 cut(s) 704
Tru1I TTAA 2 cut(s) 485, 740
Tru9I TTAA 2 cut(s) 485, 740
TseFI GTSAC 2 cut(s) 324, 652
TseI GCWGC 1 cut(s) 162
Tsp45I GTSAC 2 cut(s) 324, 652
TspDTI ATGAA 3 cut(s) 52, 79, 380
VpaK11BI GGWCC 2 cut(s) 188, 390
XapI RAATTY 2 cut(s) 767, 823
XbaI TCTAGA 1 cut(s) 22
XceI RCATGY 2 cut(s) 476, 660
XspI CTAG 2 cut(s) 23, 195
ZrmI AGTACT 1 cut(s) 199
Zsp2I ATGCAT 3 cut(s) 238, 385, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.