Rw5G035090

Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004109
Physical Location & Seq
Reverse (-)
60577031 .. 60578580
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G035090.1

Sequence Viewer

Length: 984 bp
ATGGATGGGATGGGTGCATCCGGAAACCATGATCTTAGAGGTCACAGCGTCATTAATGACTTTGAGTTACAAGATTTCGTAGATGATGCAAACTTCGACCAGTTCATCGATCTTATTCGTGGAGAAAATGAAGATCCAGCAGCTCAGTTTAATCTTGACCTTACCAGCGAGTCATTCTTTGATAACCAACTTTGTCCAACTACTGATACGGTCAGTTTTTATGGTTTTGATCATAATCTTACAATCCTATGCGATCCACCTCCCTTTCTTACTACATTACCGAGTTTTGGTGGAGAAATGAACGACATTGGCGAAGAAGATAATGATGGGGGAGATTCGTGTGGGACAACGACAACGACAACAACAACCACCACAACCACAACGACGAAGAAGCCAAAGGTTGATCGGTCGAGAACCTTGGTTTCGGAAAGAAGGAGAAGGGGAAGGATGAAGGAAAAACTCTATGCATTGCGTTCCTTGGTTCCTAACATCACTAAGATGGATAAGGCGTCCATTATTGGAGATGCTGTATTGTATGTGCAAGACCTGGAAAAGCAGGCTAAGAAGCTGAGAGCTGAGATTTTGGGACTTGAAGCATCCTTAGAAGGATCAGAGGAAAATCAAGGATCAACCCAAAATCCAACCAAGAATAAATTAGTTACAGACAATTACCATTTAATTCCCAAGGGAACCATTCAGATAGATGTGTCTCAAGTAAAGGAGAAAGGCTTTCATGTGAAAGTGGGATGCAACAGAGGAGTAGGGGTGGCCACAGCACTTTACAAGGCCATAGAATCCCTTACAAGCTTCACTGTTCAGAGTTCAAACTTGAACATGGTTGATTCCAAGAGATTCGAAATAACACTCGTCTTGAACGTAAGCTTTCGGTTTCTTCTTTATCGACATCACTGTAACCCAAATTTGAAACTTTGGGTGACTGGGGCTTTTCTGAACCAGGGTTTTGAATTAGCTACAATCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

327

Amino Acids

36.37

Weight (kDa)

5.25

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 140 - 184 6.6e-09 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 20
AclWI GGATC 4 cut(s) 128, 248, 616, 634
AcoI YGGCCR 1 cut(s) 768
AcsI RAATTY 1 cut(s) 919
AcyI GRCGYC 1 cut(s) 509
AfiI CCNNNNNNNGG 1 cut(s) 287
AgsI TTSAA 6 cut(s) 593, 825, 832, 874, 925, 965
AjnI CCWGG 2 cut(s) 546, 954
AluBI AGCT 6 cut(s) 143, 568, 575, 807, 882, 971
AluI AGCT 6 cut(s) 143, 568, 575, 807, 882, 971
Alw26I GTCTC 1 cut(s) 714
AlwI GGATC 4 cut(s) 128, 248, 616, 634
Aor13HI TCCGGA 1 cut(s) 20
AoxI GGCC 2 cut(s) 768, 786
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 1 cut(s) 919
AseI ATTAAT 1 cut(s) 54
AsuHPI GGTGA 1 cut(s) 946
AsuII TTCGAA 1 cut(s) 855
BalI TGGCCA 1 cut(s) 770
BbvI GCAGC 1 cut(s) 152
BccI CCATC 3 cut(s) 4, 320, 493
BciT130I CCWGG 2 cut(s) 548, 956
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 714
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 2 cut(s) 548, 956
BmiI GGNNCC 2 cut(s) 483, 691
BmrFI CCNGG 2 cut(s) 548, 956
BmrI ACTGGG 1 cut(s) 948
BmsI GCATC 5 cut(s) 26, 76, 514, 605, 737
BmuI ACTGGG 1 cut(s) 948
Bpu14I TTCGAA 1 cut(s) 855
BpuEI CTTGAG 1 cut(s) 696
Bsa29I ATCGAT 1 cut(s) 108
BsaBI GATNNNNATC 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 509
BsaJI CCNNGG 4 cut(s) 417, 477, 684, 955
BsaWI WCCGGW 1 cut(s) 20
BsaXI ACNNNNNCTCC 4 cut(s) 513, 543, 750, 780
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 100, 943
Bse3DI GCAATG 1 cut(s) 467
Bse8I GATNNNNATC 1 cut(s) 234
BseAI TCCGGA 1 cut(s) 20
BseBI CCWGG 2 cut(s) 548, 956
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 4 cut(s) 417, 477, 684, 955
BseGI GGATG 6 cut(s) 10, 15, 17, 453, 596, 752
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 1 cut(s) 467
BseMII CTCAG 3 cut(s) 158, 560, 567
BseNI ACTGG 2 cut(s) 100, 943
BseRI GAGGAG 1 cut(s) 771
BseXI GCAGC 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 410
BshFI GGCC 2 cut(s) 770, 788
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 410
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 2 cut(s) 358, 600
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 714
BsmFI GGGAC 2 cut(s) 358, 600
BsnI GGCC 2 cut(s) 770, 788
Bsp119I TTCGAA 1 cut(s) 855
Bsp13I TCCGGA 1 cut(s) 20
Bsp143I GATC 8 cut(s) 31, 109, 133, 229, 253, 403, 608, 626
BspANI GGCC 2 cut(s) 770, 788
BspCNI CTCAG 3 cut(s) 157, 561, 568
BspDI ATCGAT 1 cut(s) 108
BspEI TCCGGA 1 cut(s) 20
BspLI GGNNCC 2 cut(s) 483, 691
BspPI GGATC 4 cut(s) 128, 248, 616, 634
BspT104I TTCGAA 1 cut(s) 855
BsrDI GCAATG 1 cut(s) 467
BsrI ACTGG 2 cut(s) 100, 943
BssECI CCNNGG 4 cut(s) 417, 477, 684, 955
BssMI GATC 8 cut(s) 31, 109, 133, 229, 253, 403, 608, 626
BssNI GRCGYC 1 cut(s) 509
BssT1I CCWWGG 3 cut(s) 417, 477, 684
Bst2UI CCWGG 2 cut(s) 548, 956
Bst4CI ACNGT 3 cut(s) 211, 814, 911
BstACI GRCGYC 1 cut(s) 509
BstBI TTCGAA 1 cut(s) 855
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 7 cut(s) 35, 144, 495, 561, 569, 576, 601
BstF5I GGATG 6 cut(s) 10, 15, 17, 453, 596, 752
BstKTI GATC 8 cut(s) 34, 112, 136, 232, 256, 406, 611, 629
BstMAI GTCTC 1 cut(s) 714
BstMBI GATC 8 cut(s) 31, 109, 133, 229, 253, 403, 608, 626
BstMCI CGRYCG 1 cut(s) 410
BstNI CCWGG 2 cut(s) 548, 956
BstSCI CCNGG 2 cut(s) 546, 954
BstV1I GCAGC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
Bsu15I ATCGAT 1 cut(s) 108
BsuRI GGCC 2 cut(s) 770, 788
BsuTUI ATCGAT 1 cut(s) 108
BtsCI GGATG 6 cut(s) 10, 15, 17, 453, 596, 752
BtsIMutI CAGTG 2 cut(s) 810, 907
Cac8I GCNNGC 1 cut(s) 558
ClaI ATCGAT 1 cut(s) 108
CseI GACGC 2 cut(s) 37, 498
CviAII CATG 3 cut(s) 29, 734, 835
DdeI CTNAG 7 cut(s) 35, 144, 495, 561, 569, 576, 601
DpnI GATC 8 cut(s) 33, 111, 135, 231, 255, 405, 610, 628
DpnII GATC 8 cut(s) 31, 109, 133, 229, 253, 403, 608, 626
EaeI YGGCCR 1 cut(s) 768
Eco130I CCWWGG 3 cut(s) 417, 477, 684
EcoRII CCWGG 2 cut(s) 546, 954
EcoT14I CCWWGG 3 cut(s) 417, 477, 684
EcoT22I ATGCAT 1 cut(s) 469
ErhI CCWWGG 3 cut(s) 417, 477, 684
FaeI CATG 3 cut(s) 32, 737, 838
FaiI YATR 9 cut(s) 30, 222, 234, 250, 465, 537, 735, 791, 836
FaqI GGGAC 2 cut(s) 358, 600
FatI CATG 3 cut(s) 28, 733, 834
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 6 cut(s) 4, 17, 22, 460, 583, 759
Fsp4HI GCNGC 1 cut(s) 141
GluI GCNGC 1 cut(s) 141
HaeIII GGCC 2 cut(s) 770, 788
HapII CCGG 1 cut(s) 21
HgaI GACGC 2 cut(s) 37, 498
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 3 cut(s) 32, 737, 838
HindIII AAGCTT 2 cut(s) 805, 880
HinfI GANTC 5 cut(s) 170, 335, 794, 842, 852
HpaII CCGG 1 cut(s) 21
HphI GGTGA 1 cut(s) 946
Hpy188I TCNGA 5 cut(s) 427, 613, 699, 819, 951
Hpy188III TCNNGA 4 cut(s) 21, 155, 411, 871
Hpy99I CGWCG 1 cut(s) 388
HpyAV CCTTC 5 cut(s) 426, 432, 438, 445, 599
HpyCH4III ACNGT 3 cut(s) 211, 814, 911
HpyCH4IV ACGT 1 cut(s) 876
HpyCH4V TGCA 5 cut(s) 17, 89, 467, 541, 750
HpyF3I CTNAG 7 cut(s) 35, 144, 495, 561, 569, 576, 601
HpySE526I ACGT 1 cut(s) 876
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 3 cut(s) 32, 737, 838
Kpn2I TCCGGA 1 cut(s) 20
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 8 cut(s) 31, 109, 133, 229, 253, 403, 608, 626
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 5 cut(s) 26, 76, 514, 605, 737
MaeII ACGT 1 cut(s) 876
MaeIII GTNAC 5 cut(s) 41, 66, 658, 911, 934
MalI GATC 8 cut(s) 33, 111, 135, 231, 255, 405, 610, 628
MboI GATC 8 cut(s) 31, 109, 133, 229, 253, 403, 608, 626
MboII GAAGA 5 cut(s) 143, 326, 329, 400, 884
MflI RGATCY 1 cut(s) 133
MlsI TGGCCA 1 cut(s) 770
MluCI AATT 5 cut(s) 653, 667, 678, 919, 965
MluNI TGGCCA 1 cut(s) 770
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 2 cut(s) 221, 665
MnlI CCTC 4 cut(s) 32, 270, 607, 749
Mox20I TGGCCA 1 cut(s) 770
Mph1103I ATGCAT 1 cut(s) 469
MroI TCCGGA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 770
MseI TTAA 3 cut(s) 54, 150, 677
MslI CAYNNNNRTG 1 cut(s) 497
Msp20I TGGCCA 1 cut(s) 770
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 2 cut(s) 548, 956
MvaI CCWGG 2 cut(s) 548, 956
NdeII GATC 8 cut(s) 31, 109, 133, 229, 253, 403, 608, 626
NlaIII CATG 3 cut(s) 32, 737, 838
NlaIV GGNNCC 2 cut(s) 483, 691
NmuCI GTSAC 2 cut(s) 41, 934
NsiI ATGCAT 1 cut(s) 469
NspV TTCGAA 1 cut(s) 855
PcsI WCGNNNNNNNCGW 2 cut(s) 309, 873
PfeI GAWTC 4 cut(s) 335, 794, 842, 852
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PshBI ATTAAT 1 cut(s) 54
Psp6I CCWGG 2 cut(s) 546, 954
PspGI CCWGG 2 cut(s) 546, 954
PspN4I GGNNCC 2 cut(s) 483, 691
PsuI RGATCY 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 497
SaqAI TTAA 3 cut(s) 54, 150, 677
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 8 cut(s) 31, 109, 133, 229, 253, 403, 608, 626
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 2 cut(s) 548, 956
SfaNI GCATC 5 cut(s) 26, 76, 514, 605, 737
SfuI TTCGAA 1 cut(s) 855
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 1 cut(s) 711
SmoI CTYRAG 1 cut(s) 711
Sse9I AATT 5 cut(s) 653, 667, 678, 919, 965
StyD4I CCNGG 2 cut(s) 546, 954
StyI CCWWGG 3 cut(s) 417, 477, 684
TaaI ACNGT 3 cut(s) 211, 814, 911
TaiI ACGT 1 cut(s) 879
TaqI TCGA 5 cut(s) 96, 108, 410, 855, 901
TaqII GACCGA 1 cut(s) 396
TasI AATT 5 cut(s) 653, 667, 678, 919, 965
TfiI GAWTC 4 cut(s) 335, 794, 842, 852
Tru1I TTAA 3 cut(s) 54, 150, 677
Tru9I TTAA 3 cut(s) 54, 150, 677
TscAI CASTG 2 cut(s) 817, 914
TseFI GTSAC 2 cut(s) 41, 934
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 2 cut(s) 41, 934
TspDTI ATGAA 5 cut(s) 94, 144, 314, 464, 722
TspRI CASTG 2 cut(s) 817, 914
VspI ATTAAT 1 cut(s) 54
XapI RAATTY 1 cut(s) 919
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.