MD03G1127600.v1.1

Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004109
Physical Location & Seq
Forward (+)
12394059 .. 12395298
1240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1127600.v1.1.491

Sequence Viewer

Length: 918 bp
ATGGATGCGCTTGGAAACCATCTAGAATGTCATAACATCAACAACTCTGAGTTACATGATTTCATAGACGATGAAAACTTTGATCAATTCATTGATCTTATTCGCGGTGGCGAAGGTGAAGATATAGCTAATTTTGATTGTGAACTTATGATCAATGGATGCTTGGATGATTATAACCTGTTCGGTCCAGCTAGTACTACTCCTCCAGGTCCTATGTTTGGTTTCAATGATGCATTTCTACCTGACCCGAGCACTTTTGTGCTTACTACATTACCAAATTTTGATGAAGAAGACTATAATGATGGGGAAGATTCGTCTGAAACAACAAACACAAACACAACCACGACGACAACAAAGAGGCAAAAGGTTGATCGGTCACGAACTTTGGTGTCGGAGAGGAAGAGGAGGGGGAGAATGAAGGAAATGCTCTATGCATTGCGGTCCTTGGTTCCTAACATAACTAAGATGGACAAGGCCTCCATTGTTGGAGATGCTGTATTGTATGTACAAGACTTGCAAATGCATGCTAAGAAGTTAAATGCTGAGATTGCAAGCCTTGAAGCATCATTAGCAGGAGCAGATGAGCATATGGGATATCTACAAGGATCAACCAAAACTAAAAACAAGAAAAAAGTTTCAGACAATAACCATTTATTTTCTAAGGGCATCATTCAGATAGACGGGTCTCAAGTGGAGGAAAAAGGTTTTTATGTGAAGGTGGCATGCAACAAGGTACAAGGAGTAGCCACAGCACTTTACAAGGCTCTCGAATCTCTTGCATGCTTCAATGTTCAGAGTTCTAACTTAAACACAGTTTCAGCAGACAGATTTGAATTAGCATTCACTTTGAATGTATGTATATATCAGTACAATATGATATTCTACTCTATCCTTGGTCGGCGATTTTTACAGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

306

Amino Acids

34.12

Weight (kDa)

4.93

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 129 - 173 1.5e-09 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 174
AccII CGCG 1 cut(s) 105
AciI CCGC 2 cut(s) 105, 439
AclWI GGATC 1 cut(s) 613
AcsI RAATTY 1 cut(s) 277
AfaI GTAC 4 cut(s) 196, 507, 735, 869
AfiI CCNNNNNNNGG 1 cut(s) 218
AgsI TTSAA 5 cut(s) 226, 560, 787, 833, 850
AjnI CCWGG 1 cut(s) 205
AleI CACNNNNGTG 1 cut(s) 257
AluBI AGCT 2 cut(s) 128, 191
AluI AGCT 2 cut(s) 128, 191
Alw21I GWGCWC 1 cut(s) 254
Alw26I GTCTC 1 cut(s) 690
AlwI GGATC 1 cut(s) 613
Ama87I CYCGRG 1 cut(s) 247
AoxI GGCC 1 cut(s) 474
ApoI RAATTY 1 cut(s) 277
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 3 cut(s) 185, 209, 441
AsuHPI GGTGA 1 cut(s) 128
AvaI CYCGRG 1 cut(s) 247
AvaII GGWCC 3 cut(s) 185, 209, 441
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 254
BccI CCATC 3 cut(s) 27, 296, 460
BcgI CGANNNNNNTGC 2 cut(s) 758, 792
BciT130I CCWGG 1 cut(s) 207
BclI TGATCA 2 cut(s) 82, 150
BcoDI GTCTC 1 cut(s) 690
BfaI CTAG 2 cut(s) 23, 192
BmcAI AGTACT 1 cut(s) 196
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 3 cut(s) 185, 209, 441
BmeT110I CYCGRG 1 cut(s) 247
BmgT120I GGNCC 3 cut(s) 185, 209, 441
BmiI GGNNCC 1 cut(s) 450
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 5 cut(s) 149, 220, 481, 572, 675
BpiI GAAGAC 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 189
BpuEI CTTGAG 1 cut(s) 672
BsaI GGTCTC 1 cut(s) 690
BsaJI CCNNGG 2 cut(s) 444, 892
BsaXI ACNNNNNCTCC 6 cut(s) 187, 217, 461, 480, 491, 510
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse3DI GCAATG 1 cut(s) 434
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 444, 892
BseGI GGATG 3 cut(s) 10, 164, 172
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMI GCAATG 1 cut(s) 434
BseMII CTCAG 2 cut(s) 39, 534
BseRI GAGGAG 2 cut(s) 192, 418
Bsh1236I CGCG 1 cut(s) 105
BshFI GGCC 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 254
BsiHKCI CYCGRG 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 1 cut(s) 690
BsmI GAATGC 1 cut(s) 839
BsnI GGCC 1 cut(s) 476
Bso31I GGTCTC 1 cut(s) 690
BsoBI CYCGRG 1 cut(s) 247
Bsp1286I GDGCHC 1 cut(s) 254
Bsp1407I TGTACA 1 cut(s) 505
Bsp143I GATC 5 cut(s) 82, 94, 150, 370, 605
BspACI CCGC 2 cut(s) 105, 439
BspANI GGCC 1 cut(s) 476
BspCNI CTCAG 2 cut(s) 40, 535
BspFNI CGCG 1 cut(s) 105
BspLI GGNNCC 1 cut(s) 450
BspPI GGATC 1 cut(s) 613
BspTNI GGTCTC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 434
BsrGI TGTACA 1 cut(s) 505
BssECI CCNNGG 2 cut(s) 444, 892
BssMI GATC 5 cut(s) 82, 94, 150, 370, 605
BssT1I CCWWGG 2 cut(s) 444, 892
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 2 cut(s) 814, 912
Bst6I CTCTTC 1 cut(s) 395
BstAUI TGTACA 1 cut(s) 505
BstC8I GCNNGC 4 cut(s) 525, 553, 724, 781
BstDEI CTNAG 5 cut(s) 48, 462, 528, 543, 660
BstF5I GGATG 3 cut(s) 10, 164, 172
BstFNI CGCG 1 cut(s) 105
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 5 cut(s) 85, 97, 153, 373, 608
BstMAI GTCTC 1 cut(s) 690
BstMBI GATC 5 cut(s) 82, 94, 150, 370, 605
BstMWI GCNNNNNNNGC 2 cut(s) 548, 569
BstNI CCWGG 1 cut(s) 207
BstNSI RCATGY 3 cut(s) 527, 726, 783
BstSCI CCNGG 1 cut(s) 205
BstUI CGCG 1 cut(s) 105
BstV2I GAAGAC 1 cut(s) 297
BsuRI GGCC 1 cut(s) 476
BtsCI GGATG 3 cut(s) 10, 164, 172
Cac8I GCNNGC 4 cut(s) 525, 553, 724, 781
CfoI GCGC 1 cut(s) 10
Cfr13I GGNCC 3 cut(s) 185, 209, 441
Csp6I GTAC 4 cut(s) 195, 506, 734, 868
CviAII CATG 4 cut(s) 56, 524, 723, 780
CviJI RGCY 6 cut(s) 128, 191, 476, 555, 746, 764
CviKI_1 RGCY 6 cut(s) 128, 191, 476, 555, 746, 764
CviQI GTAC 4 cut(s) 195, 506, 734, 868
DdeI CTNAG 5 cut(s) 48, 462, 528, 543, 660
DpnI GATC 5 cut(s) 84, 96, 152, 372, 607
DpnII GATC 5 cut(s) 82, 94, 150, 370, 605
Eam1104I CTCTTC 1 cut(s) 395
EarI CTCTTC 1 cut(s) 395
Eco130I CCWWGG 2 cut(s) 444, 892
Eco147I AGGCCT 1 cut(s) 476
Eco31I GGTCTC 1 cut(s) 690
Eco32I GATATC 1 cut(s) 596
Eco47I GGWCC 3 cut(s) 185, 209, 441
Eco88I CYCGRG 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 205
EcoRV GATATC 1 cut(s) 596
EcoT14I CCWWGG 2 cut(s) 444, 892
EcoT22I ATGCAT 3 cut(s) 235, 436, 525
ErhI CCWWGG 2 cut(s) 444, 892
FaeI CATG 4 cut(s) 59, 527, 726, 783
FatI CATG 4 cut(s) 55, 523, 722, 779
FauNDI CATATG 1 cut(s) 588
FbaI TGATCA 2 cut(s) 82, 150
FokI GGATG 3 cut(s) 17, 171, 179
FspBI CTAG 2 cut(s) 23, 192
GlaI GCGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 189
HaeIII GGCC 1 cut(s) 476
HhaI GCGC 1 cut(s) 10
Hin1II CATG 4 cut(s) 59, 527, 726, 783
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HinfI GANTC 2 cut(s) 311, 770
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 1 cut(s) 143
Hpy188I TCNGA 6 cut(s) 49, 319, 394, 640, 675, 795
Hpy188III TCNNGA 3 cut(s) 23, 378, 767
Hpy8I GTNNAC 1 cut(s) 143
Hpy99I CGWCG 1 cut(s) 349
HpyAV CCTTC 3 cut(s) 107, 412, 709
HpyCH4III ACNGT 2 cut(s) 814, 912
HpyCH4V TGCA 7 cut(s) 233, 434, 517, 523, 551, 726, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 548, 569
HpyF3I CTNAG 5 cut(s) 48, 462, 528, 543, 660
Hsp92II CATG 4 cut(s) 59, 527, 726, 783
HspAI GCGC 1 cut(s) 8
Ksp22I TGATCA 2 cut(s) 82, 150
Kzo9I GATC 5 cut(s) 82, 94, 150, 370, 605
LmnI GCTCC 1 cut(s) 575
LpnPI CCDG 6 cut(s) 191, 192, 201, 219, 255, 558
LweI GCATC 5 cut(s) 149, 220, 481, 572, 675
MaeI CTAG 2 cut(s) 23, 192
MaeIII GTNAC 2 cut(s) 51, 375
MalI GATC 5 cut(s) 84, 96, 152, 372, 607
MboI GATC 5 cut(s) 82, 94, 150, 370, 605
MboII GAAGA 5 cut(s) 131, 299, 302, 320, 412
MhlI GDGCHC 1 cut(s) 254
MluCI AATT 4 cut(s) 86, 130, 277, 833
MmeI TCCRAC 2 cut(s) 372, 466
MnlI CCTC 7 cut(s) 213, 351, 390, 396, 399, 487, 688
Mph1103I ATGCAT 3 cut(s) 235, 436, 525
MseI TTAA 2 cut(s) 536, 806
MslI CAYNNNNRTG 1 cut(s) 257
MspR9I CCNGG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 839
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 2 cut(s) 548, 569
NdeI CATATG 1 cut(s) 588
NdeII GATC 5 cut(s) 82, 94, 150, 370, 605
NlaIII CATG 4 cut(s) 59, 527, 726, 783
NlaIV GGNNCC 1 cut(s) 450
NmuCI GTSAC 1 cut(s) 375
NsiI ATGCAT 3 cut(s) 235, 436, 525
NspI RCATGY 3 cut(s) 527, 726, 783
OliI CACNNNNGTG 1 cut(s) 257
PaeI GCATGC 3 cut(s) 527, 726, 783
PceI AGGCCT 1 cut(s) 476
PctI GAATGC 1 cut(s) 839
PfeI GAWTC 2 cut(s) 311, 770
PpuMI RGGWCCY 1 cut(s) 209
PsiI TTATAA 1 cut(s) 174
Psp5II RGGWCCY 1 cut(s) 209
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 450
PspPI GGNCC 3 cut(s) 185, 209, 441
PspPPI RGGWCCY 1 cut(s) 209
RsaI GTAC 4 cut(s) 196, 507, 735, 869
RsaNI GTAC 4 cut(s) 195, 506, 734, 868
RseI CAYNNNNRTG 1 cut(s) 257
SaqAI TTAA 2 cut(s) 536, 806
Sau3AI GATC 5 cut(s) 82, 94, 150, 370, 605
Sau96I GGNCC 3 cut(s) 185, 209, 441
ScaI AGTACT 1 cut(s) 196
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 1 cut(s) 254
SfaNI GCATC 5 cut(s) 149, 220, 481, 572, 675
SinI GGWCC 3 cut(s) 185, 209, 441
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 687
SmoI CTYRAG 1 cut(s) 687
SphI GCATGC 3 cut(s) 527, 726, 783
Sse9I AATT 4 cut(s) 86, 130, 277, 833
SseBI AGGCCT 1 cut(s) 476
SsiI CCGC 2 cut(s) 105, 439
SspMI CTAG 2 cut(s) 23, 192
StuI AGGCCT 1 cut(s) 476
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 2 cut(s) 444, 892
TaaI ACNGT 2 cut(s) 814, 912
TaqI TCGA 1 cut(s) 768
TaqII GACCGA 2 cut(s) 173, 363
TasI AATT 4 cut(s) 86, 130, 277, 833
TatI WGTACW 3 cut(s) 194, 505, 867
TfiI GAWTC 2 cut(s) 311, 770
Tru1I TTAA 2 cut(s) 536, 806
Tru9I TTAA 2 cut(s) 536, 806
TseFI GTSAC 1 cut(s) 375
Tsp45I GTSAC 1 cut(s) 375
TspDTI ATGAA 5 cut(s) 52, 79, 87, 300, 431
VpaK11BI GGWCC 3 cut(s) 185, 209, 441
XapI RAATTY 1 cut(s) 277
XbaI TCTAGA 1 cut(s) 22
XceI RCATGY 3 cut(s) 527, 726, 783
XspI CTAG 2 cut(s) 23, 192
ZrmI AGTACT 1 cut(s) 196
Zsp2I ATGCAT 3 cut(s) 235, 436, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.