MD03G1129100.v1.1

Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004109
Physical Location & Seq
Reverse (-)
12560753 .. 12562473
1721 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1129100.v1.1.491

Sequence Viewer

Length: 972 bp
ATGGATTCGCTGGGAAACCATCAAGGAGGTCATAACATCAACGATTTTGAGTTGCAAGACTTCATAGATGATGCAAACTTTGGTCAATTCATTGATATTATTCGCGGTGACGGTGAAGATCCAGCGGCTAATTTTGATCCTGACCTTATGATGAATGGCTGCTTTGATGATTATAACCTGTTTGGTCAAGCTGGTTCTACTACTCCACCAGTTCTTATGTTTGGTTTTAATGATGCAATTGTACCCGATCCAACTACTTCTTTGTTTGCTACATCACCAAATTTTGATGGAGAAATGAAAGGCGGGGAAGAAGAGTACTATAATGACGGGGAAGATTCGTCTGGAACAACCACAACCATGACAACAACGAAGAGGCAAAAGGTTGACCGGTCGCGGACTTTGGTGTCGGAGAGGAAGAGGAGGGGGAGCATGAAGGAAAGGCTATATGCATTGCGGTCTTTAGTTCCTAACATAACCAAGATGGATAAGGCCTCCATTGTTGGAGATTCTGTATTGTATGTACAAGACTTGCAACAGCAGGCTAAGAAGTTGAAAGCTGAGATTGCAAGCCTTGAAGCATCCTTAGCCGGAGCAGATGATCGAGACGGACATCTGGAAGGATCAACCAAACCTAATAAAGATTCAAACAATGACCAGTTCGTTTCTAAGGGCATCCTTCAGGTTAGAATAGACGTGTCTCAAGTGGAGGAAAAAGGGTTTTATGTGAAGGTGGCATGCAACAAGGGAGGAGGAGTAGCCATATCACTTTACAAGGCACTTGAATCCCTAACAAGCTTCGATGTTCAAAGTTCTAACTTGAACACAGTTTCAGCTGACAGATTTGAAATCACATTTGCTTTGAATGTAAAGAAATGCGAGCAGGACGTCGTCAACCTGCCAAATTTGAAACTTTGGGTCACGGGTGCTTTTCTTAATCAAGGTTTTAAATTGGCTTCTGGATTCTCAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

324

Amino Acids

35.37

Weight (kDa)

4.73

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 134 - 178 2.4e-08 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 174
AasI GACNNNNNNGTC 1 cut(s) 403
AatII GACGTC 1 cut(s) 888
Acc36I ACCTGC 1 cut(s) 903
AccII CGCG 2 cut(s) 105, 394
AciI CCGC 5 cut(s) 105, 125, 303, 394, 454
AclWI GGATC 4 cut(s) 113, 131, 242, 628
AcsI RAATTY 2 cut(s) 280, 901
AcuI CTGAAG 1 cut(s) 662
AcyI GRCGYC 1 cut(s) 885
AfaI GTAC 3 cut(s) 243, 317, 522
AflIII ACRYGT 1 cut(s) 693
AgeI ACCGGT 1 cut(s) 387
AgsI TTSAA 9 cut(s) 553, 575, 645, 782, 806, 820, 845, 862, 907
AjiI CACGTC 1 cut(s) 694
AluBI AGCT 4 cut(s) 191, 557, 795, 833
AluI AGCT 4 cut(s) 191, 557, 795, 833
Alw26I GTCTC 2 cut(s) 597, 702
AlwI GGATC 4 cut(s) 113, 131, 242, 628
AoxI GGCC 1 cut(s) 489
ApeKI GCWGC 1 cut(s) 159
ApoI RAATTY 2 cut(s) 280, 901
AsiGI ACCGGT 1 cut(s) 387
AsuHPI GGTGA 3 cut(s) 119, 125, 267
BbvI GCAGC 1 cut(s) 146
BccI CCATC 3 cut(s) 27, 281, 475
BcoDI GTCTC 2 cut(s) 597, 702
BfuAI ACCTGC 1 cut(s) 903
BisI GCNGC 2 cut(s) 126, 160
BlsI GCNGC 2 cut(s) 127, 161
BmcAI AGTACT 1 cut(s) 317
BmgBI CACGTC 1 cut(s) 694
BmsI GCATC 4 cut(s) 61, 223, 587, 681
Bpu10I CCTNAGC 1 cut(s) 583
BpuEI CTTGAG 1 cut(s) 684
BsaHI GRCGYC 1 cut(s) 885
BsaWI WCCGGW 1 cut(s) 387
BsaXI ACNNNNNCTCC 4 cut(s) 495, 525, 738, 768
Bse118I RCCGGY 1 cut(s) 387
Bse1I ACTGG 2 cut(s) 209, 655
Bse3DI GCAATG 1 cut(s) 449
BseGI GGATG 2 cut(s) 578, 672
BseMI GCAATG 1 cut(s) 449
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 2 cut(s) 209, 655
BseRI GAGGAG 3 cut(s) 433, 762, 765
BseXI GCAGC 1 cut(s) 146
BseYI CCCAGC 1 cut(s) 10
Bsh1236I CGCG 2 cut(s) 105, 394
Bsh1285I CGRYCG 1 cut(s) 392
BshFI GGCC 1 cut(s) 491
BshTI ACCGGT 1 cut(s) 387
BsiEI CGRYCG 1 cut(s) 392
BsiSI CCGG 2 cut(s) 388, 588
BsmAI GTCTC 2 cut(s) 597, 702
BsmBI CGTCTC 1 cut(s) 597
BsnI GGCC 1 cut(s) 491
Bsp1407I TGTACA 1 cut(s) 520
Bsp143I GATC 5 cut(s) 118, 136, 247, 598, 620
BspACI CCGC 5 cut(s) 105, 125, 303, 394, 454
BspANI GGCC 1 cut(s) 491
BspCNI CTCAG 1 cut(s) 550
BspFNI CGCG 2 cut(s) 105, 394
BspMI ACCTGC 1 cut(s) 903
BspPI GGATC 4 cut(s) 113, 131, 242, 628
BsrDI GCAATG 1 cut(s) 449
BsrFI RCCGGY 1 cut(s) 387
BsrGI TGTACA 1 cut(s) 520
BsrI ACTGG 2 cut(s) 209, 655
BssAI RCCGGY 1 cut(s) 387
BssMI GATC 5 cut(s) 118, 136, 247, 598, 620
BssNI GRCGYC 1 cut(s) 885
Bst4CI ACNGT 2 cut(s) 113, 826
Bst6I CTCTTC 3 cut(s) 306, 365, 410
BstACI GRCGYC 1 cut(s) 885
BstAUI TGTACA 1 cut(s) 520
BstC8I GCNNGC 4 cut(s) 540, 568, 736, 878
BstDEI CTNAG 5 cut(s) 543, 558, 583, 666, 964
BstF5I GGATG 2 cut(s) 578, 672
BstFNI CGCG 2 cut(s) 105, 394
BstKTI GATC 5 cut(s) 121, 139, 250, 601, 623
BstMAI GTCTC 2 cut(s) 597, 702
BstMBI GATC 5 cut(s) 118, 136, 247, 598, 620
BstMCI CGRYCG 1 cut(s) 392
BstMWI GCNNNNNNNGC 2 cut(s) 563, 584
BstNSI RCATGY 1 cut(s) 738
BstUI CGCG 2 cut(s) 105, 394
BstV1I GCAGC 1 cut(s) 146
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
BsuRI GGCC 1 cut(s) 491
BtrI CACGTC 1 cut(s) 694
BtsCI GGATG 2 cut(s) 578, 672
BveI ACCTGC 1 cut(s) 903
Cac8I GCNNGC 4 cut(s) 540, 568, 736, 878
Cfr10I RCCGGY 1 cut(s) 387
Csp6I GTAC 3 cut(s) 242, 316, 521
CspAI ACCGGT 1 cut(s) 387
CviAII CATG 3 cut(s) 358, 430, 735
CviQI GTAC 3 cut(s) 242, 316, 521
DdeI CTNAG 5 cut(s) 543, 558, 583, 666, 964
DpnI GATC 5 cut(s) 120, 138, 249, 600, 622
DpnII GATC 5 cut(s) 118, 136, 247, 598, 620
DraI TTTAAA 1 cut(s) 946
DrdI GACNNNNNNGTC 1 cut(s) 403
DseDI GACNNNNNNGTC 1 cut(s) 403
Eam1104I CTCTTC 3 cut(s) 306, 365, 410
EarI CTCTTC 3 cut(s) 306, 365, 410
Eco147I AGGCCT 1 cut(s) 491
Eco57I CTGAAG 1 cut(s) 662
EcoT22I ATGCAT 1 cut(s) 451
Esp3I CGTCTC 1 cut(s) 597
FaeI CATG 3 cut(s) 361, 433, 738
FatI CATG 3 cut(s) 357, 429, 734
FauI CCCGC 1 cut(s) 296
Fnu4HI GCNGC 2 cut(s) 126, 160
FokI GGATG 2 cut(s) 565, 659
Fsp4HI GCNGC 2 cut(s) 126, 160
GluI GCNGC 2 cut(s) 126, 160
GsaI CCCAGC 1 cut(s) 14
HaeIII GGCC 1 cut(s) 491
HapII CCGG 2 cut(s) 388, 588
Hin1I GRCGYC 1 cut(s) 885
Hin1II CATG 3 cut(s) 361, 433, 738
HincII GTYRAC 2 cut(s) 385, 892
HindII GTYRAC 2 cut(s) 385, 892
HindIII AAGCTT 1 cut(s) 793
HinfI GANTC 6 cut(s) 5, 335, 506, 641, 782, 960
HpaII CCGG 2 cut(s) 388, 588
HphI GGTGA 3 cut(s) 119, 125, 267
Hpy166II GTNNAC 2 cut(s) 385, 892
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 5 cut(s) 140, 342, 602, 614, 957
Hpy8I GTNNAC 2 cut(s) 385, 892
Hpy99I CGWCG 1 cut(s) 890
HpyAV CCTTC 4 cut(s) 427, 611, 686, 721
HpyCH4III ACNGT 2 cut(s) 113, 826
HpyCH4IV ACGT 2 cut(s) 693, 885
HpyCH4V TGCA 7 cut(s) 55, 74, 236, 449, 532, 566, 738
HpyF10VI GCNNNNNNNGC 2 cut(s) 563, 584
HpyF3I CTNAG 5 cut(s) 543, 558, 583, 666, 964
HpySE526I ACGT 2 cut(s) 693, 885
Hsp92I GRCGYC 1 cut(s) 885
Hsp92II CATG 3 cut(s) 361, 433, 738
Kzo9I GATC 5 cut(s) 118, 136, 247, 598, 620
LmnI GCTCC 2 cut(s) 426, 590
Lsp1109I GCAGC 1 cut(s) 146
LweI GCATC 4 cut(s) 61, 223, 587, 681
MaeII ACGT 2 cut(s) 693, 885
MaeIII GTNAC 2 cut(s) 107, 916
MalI GATC 5 cut(s) 120, 138, 249, 600, 622
MboI GATC 5 cut(s) 118, 136, 247, 598, 620
MboII GAAGA 6 cut(s) 128, 320, 323, 344, 382, 427
MfeI CAATTG 1 cut(s) 237
MflI RGATCY 1 cut(s) 118
MluCI AATT 6 cut(s) 86, 130, 237, 280, 901, 947
MmeI TCCRAC 3 cut(s) 275, 387, 481
MnlI CCTC 9 cut(s) 20, 366, 405, 411, 414, 502, 700, 740, 743
Mph1103I ATGCAT 1 cut(s) 451
MseI TTAA 3 cut(s) 228, 933, 945
MslI CAYNNNNRTG 1 cut(s) 356
MspA1I CMGCKG 2 cut(s) 125, 833
MspI CCGG 2 cut(s) 388, 588
MunI CAATTG 1 cut(s) 237
MvnI CGCG 2 cut(s) 105, 394
MwoI GCNNNNNNNGC 2 cut(s) 563, 584
NdeII GATC 5 cut(s) 118, 136, 247, 598, 620
NlaIII CATG 3 cut(s) 361, 433, 738
NmuCI GTSAC 2 cut(s) 107, 916
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 1 cut(s) 738
PaeI GCATGC 1 cut(s) 738
PceI AGGCCT 1 cut(s) 491
PfeI GAWTC 6 cut(s) 5, 335, 506, 641, 782, 960
PflFI GACNNNGTC 1 cut(s) 887
PinAI ACCGGT 1 cut(s) 387
PkrI GCNGC 2 cut(s) 127, 161
PsiI TTATAA 1 cut(s) 174
PspFI CCCAGC 1 cut(s) 10
PsuI RGATCY 1 cut(s) 118
PsyI GACNNNGTC 1 cut(s) 887
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 3 cut(s) 243, 317, 522
RsaNI GTAC 3 cut(s) 242, 316, 521
RseI CAYNNNNRTG 1 cut(s) 356
SaqAI TTAA 3 cut(s) 228, 933, 945
SatI GCNGC 2 cut(s) 126, 160
Sau3AI GATC 5 cut(s) 118, 136, 247, 598, 620
ScaI AGTACT 1 cut(s) 317
SfaNI GCATC 4 cut(s) 61, 223, 587, 681
SmiMI CAYNNNNRTG 1 cut(s) 356
SmlI CTYRAG 1 cut(s) 699
SmoI CTYRAG 1 cut(s) 699
SphI GCATGC 1 cut(s) 738
Sse9I AATT 6 cut(s) 86, 130, 237, 280, 901, 947
SseBI AGGCCT 1 cut(s) 491
SsiI CCGC 5 cut(s) 105, 125, 303, 394, 454
StuI AGGCCT 1 cut(s) 491
TaaI ACNGT 2 cut(s) 113, 826
TaiI ACGT 2 cut(s) 696, 888
TaqI TCGA 2 cut(s) 601, 798
TasI AATT 6 cut(s) 86, 130, 237, 280, 901, 947
TatI WGTACW 2 cut(s) 315, 520
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 6 cut(s) 5, 335, 506, 641, 782, 960
Tru1I TTAA 3 cut(s) 228, 933, 945
Tru9I TTAA 3 cut(s) 228, 933, 945
TseFI GTSAC 2 cut(s) 107, 916
TseI GCWGC 1 cut(s) 159
Tsp45I GTSAC 2 cut(s) 107, 916
TspDTI ATGAA 5 cut(s) 52, 79, 167, 311, 446
TspGWI ACGGA 1 cut(s) 621
Tth111I GACNNNGTC 1 cut(s) 887
XapI RAATTY 2 cut(s) 280, 901
XceI RCATGY 1 cut(s) 738
ZraI GACGTC 1 cut(s) 886
ZrmI AGTACT 1 cut(s) 317
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.