Rh5DG397300

Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004109
Physical Location & Seq
Reverse (-)
62612866 .. 62614762
1897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG397300.1

Sequence Viewer

Length: 912 bp
ATGGATGGGATGGGTGCATCCGGAAACCATGATCTTAGAGGTCACAGCGTCATTAATGACTTTGAGTTACAAGATTTCGTAGATGATGCAAACTTCGACCAGTTCATCGATCTTATTCGTGGAGAAAATGAAGATCCAGCAGCTCAGTTTAATCTTGACCTTACCAGCGGGTCATTCTTTGATAACCAACTTTGTCCAACTACTGATACGGTCAGTTTTTTTGGTTTTGATCATAATCTTACAATCCTATGCGATCCACCTCCCTTTCTTACTACATTACCGAGTTTTGGTGGAGAAATGAACGACATTGGCGAAGAAGATAATGATGGGGGAGATTCGTGTGGGACAACGACAACGACAACAACAACCACCACAACCACAGCCACAACGACGAAGAAGCCAAAGGTTGATCGGTCGAGAACCTTGGTTTCGGAAAGAAGGAGAAGGGGAAGGATGAAGGAAAAACTCTATGCATTGCGTTCCTTGGTTCCTAACATCACTAAGATGGATAAGGCGTCCATTATTGGAGATGCTGTATTGTATGTGCAAGACCTGGAAAAGCAGGCTAAGAAGCTGAGAGCTGAGATTTTGGGACTTGAAGCATCCTTAGAAGGATCAGAGGAAAATCAAGGATCACCCCAAAATCCAACCAAGAATAAATTAGTTACAGACAATTACCATTTAATTCCCAAGGGAACCATTCAGATAGATGTGTCTCAAGTAAAGGAGAAAGGCTTTCATGTGAAAGTGGGATGCAACAGAGGAGTCGGGGTGGCCACAGCACTTTACAAGGCCATAGAATCCCTTACAAGCTTCACTGTTCAGAGTTCAAACTTGAACATGGTTAATTCCAAGAGATTCGAAATAACACTCGCCTTGAACCACCACAACCACCGAAGCCGTGCGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

303

Amino Acids

33.43

Weight (kDa)

5.65

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 142 - 186 5.9e-09 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 907
AccIII TCCGGA 1 cut(s) 20
AciI CCGC 1 cut(s) 168
AclWI GGATC 4 cut(s) 128, 248, 622, 640
AcoI YGGCCR 1 cut(s) 774
AcyI GRCGYC 1 cut(s) 515
AfiI CCNNNNNNNGG 1 cut(s) 287
AgsI TTSAA 4 cut(s) 599, 831, 838, 880
AjnI CCWGG 1 cut(s) 552
AluBI AGCT 4 cut(s) 143, 574, 581, 813
AluI AGCT 4 cut(s) 143, 574, 581, 813
Alw26I GTCTC 1 cut(s) 720
AlwI GGATC 4 cut(s) 128, 248, 622, 640
Aor13HI TCCGGA 1 cut(s) 20
AoxI GGCC 2 cut(s) 774, 792
ApeKI GCWGC 1 cut(s) 140
AseI ATTAAT 1 cut(s) 54
AspLEI GCGC 1 cut(s) 907
AsuHPI GGTGA 1 cut(s) 627
AsuII TTCGAA 1 cut(s) 861
BalI TGGCCA 1 cut(s) 776
BbvI GCAGC 1 cut(s) 152
BccI CCATC 3 cut(s) 4, 320, 499
BceAI ACGGC 1 cut(s) 885
BciT130I CCWGG 1 cut(s) 554
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 720
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 1 cut(s) 554
BmiI GGNNCC 2 cut(s) 489, 697
BmrFI CCNGG 1 cut(s) 554
BmsI GCATC 5 cut(s) 26, 76, 520, 611, 743
Bpu14I TTCGAA 1 cut(s) 861
BpuEI CTTGAG 1 cut(s) 702
Bsa29I ATCGAT 1 cut(s) 108
BsaBI GATNNNNATC 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 515
BsaJI CCNNGG 3 cut(s) 423, 483, 690
BsaWI WCCGGW 1 cut(s) 20
BsaXI ACNNNNNCTCC 4 cut(s) 519, 549, 756, 786
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 100
Bse3DI GCAATG 1 cut(s) 473
Bse8I GATNNNNATC 1 cut(s) 234
BseAI TCCGGA 1 cut(s) 20
BseBI CCWGG 1 cut(s) 554
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 3 cut(s) 423, 483, 690
BseGI GGATG 6 cut(s) 10, 15, 17, 459, 602, 758
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 1 cut(s) 473
BseMII CTCAG 3 cut(s) 158, 566, 573
BseNI ACTGG 1 cut(s) 100
BseRI GAGGAG 1 cut(s) 777
BseXI GCAGC 1 cut(s) 152
Bsh1236I CGCG 1 cut(s) 907
Bsh1285I CGRYCG 1 cut(s) 416
BshFI GGCC 2 cut(s) 776, 794
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 416
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 2 cut(s) 358, 606
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 720
BsmFI GGGAC 2 cut(s) 358, 606
BsnI GGCC 2 cut(s) 776, 794
Bsp119I TTCGAA 1 cut(s) 861
Bsp13I TCCGGA 1 cut(s) 20
Bsp143I GATC 8 cut(s) 31, 109, 133, 229, 253, 409, 614, 632
BspACI CCGC 1 cut(s) 168
BspANI GGCC 2 cut(s) 776, 794
BspCNI CTCAG 3 cut(s) 157, 567, 574
BspDI ATCGAT 1 cut(s) 108
BspEI TCCGGA 1 cut(s) 20
BspFNI CGCG 1 cut(s) 907
BspLI GGNNCC 2 cut(s) 489, 697
BspPI GGATC 4 cut(s) 128, 248, 622, 640
BspT104I TTCGAA 1 cut(s) 861
BsrDI GCAATG 1 cut(s) 473
BsrI ACTGG 1 cut(s) 100
BssECI CCNNGG 3 cut(s) 423, 483, 690
BssMI GATC 8 cut(s) 31, 109, 133, 229, 253, 409, 614, 632
BssNI GRCGYC 1 cut(s) 515
BssT1I CCWWGG 3 cut(s) 423, 483, 690
Bst2UI CCWGG 1 cut(s) 554
Bst4CI ACNGT 2 cut(s) 211, 820
BstACI GRCGYC 1 cut(s) 515
BstBI TTCGAA 1 cut(s) 861
BstC8I GCNNGC 1 cut(s) 564
BstDEI CTNAG 7 cut(s) 35, 144, 501, 567, 575, 582, 607
BstF5I GGATG 6 cut(s) 10, 15, 17, 459, 602, 758
BstFNI CGCG 1 cut(s) 907
BstHHI GCGC 1 cut(s) 907
BstKTI GATC 8 cut(s) 34, 112, 136, 232, 256, 412, 617, 635
BstMAI GTCTC 1 cut(s) 720
BstMBI GATC 8 cut(s) 31, 109, 133, 229, 253, 409, 614, 632
BstMCI CGRYCG 1 cut(s) 416
BstNI CCWGG 1 cut(s) 554
BstSCI CCNGG 1 cut(s) 552
BstUI CGCG 1 cut(s) 907
BstV1I GCAGC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
Bsu15I ATCGAT 1 cut(s) 108
BsuRI GGCC 2 cut(s) 776, 794
BsuTUI ATCGAT 1 cut(s) 108
BtsCI GGATG 6 cut(s) 10, 15, 17, 459, 602, 758
BtsIMutI CAGTG 1 cut(s) 816
Cac8I GCNNGC 1 cut(s) 564
CfoI GCGC 1 cut(s) 907
ClaI ATCGAT 1 cut(s) 108
CseI GACGC 2 cut(s) 37, 504
CviAII CATG 3 cut(s) 29, 740, 841
DdeI CTNAG 7 cut(s) 35, 144, 501, 567, 575, 582, 607
DpnI GATC 8 cut(s) 33, 111, 135, 231, 255, 411, 616, 634
DpnII GATC 8 cut(s) 31, 109, 133, 229, 253, 409, 614, 632
EaeI YGGCCR 1 cut(s) 774
Eco130I CCWWGG 3 cut(s) 423, 483, 690
EcoRII CCWGG 1 cut(s) 552
EcoT14I CCWWGG 3 cut(s) 423, 483, 690
EcoT22I ATGCAT 1 cut(s) 475
ErhI CCWWGG 3 cut(s) 423, 483, 690
FaeI CATG 3 cut(s) 32, 743, 844
FaiI YATR 8 cut(s) 30, 234, 250, 471, 543, 741, 797, 842
FaqI GGGAC 2 cut(s) 358, 606
FatI CATG 3 cut(s) 28, 739, 840
FauI CCCGC 1 cut(s) 161
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 6 cut(s) 4, 17, 22, 466, 589, 765
Fsp4HI GCNGC 1 cut(s) 141
GlaI GCGC 1 cut(s) 906
GluI GCNGC 1 cut(s) 141
HaeIII GGCC 2 cut(s) 776, 794
HapII CCGG 1 cut(s) 21
HgaI GACGC 2 cut(s) 37, 504
HhaI GCGC 1 cut(s) 907
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 3 cut(s) 32, 743, 844
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HindIII AAGCTT 1 cut(s) 811
HinfI GANTC 4 cut(s) 335, 765, 800, 858
HpaII CCGG 1 cut(s) 21
HphI GGTGA 1 cut(s) 627
Hpy188I TCNGA 4 cut(s) 433, 619, 705, 825
Hpy188III TCNNGA 3 cut(s) 21, 155, 417
Hpy99I CGWCG 1 cut(s) 394
HpyAV CCTTC 5 cut(s) 432, 438, 444, 451, 605
HpyCH4III ACNGT 2 cut(s) 211, 820
HpyCH4V TGCA 5 cut(s) 17, 89, 473, 547, 756
HpyF3I CTNAG 7 cut(s) 35, 144, 501, 567, 575, 582, 607
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 3 cut(s) 32, 743, 844
HspAI GCGC 1 cut(s) 905
Kpn2I TCCGGA 1 cut(s) 20
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 8 cut(s) 31, 109, 133, 229, 253, 409, 614, 632
LpnPI CCDG 7 cut(s) 34, 113, 150, 178, 539, 548, 566
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 5 cut(s) 26, 76, 520, 611, 743
MaeIII GTNAC 3 cut(s) 41, 66, 664
MalI GATC 8 cut(s) 33, 111, 135, 231, 255, 411, 616, 634
MboI GATC 8 cut(s) 31, 109, 133, 229, 253, 409, 614, 632
MboII GAAGA 4 cut(s) 143, 326, 329, 406
MflI RGATCY 1 cut(s) 133
MlsI TGGCCA 1 cut(s) 776
MluCI AATT 4 cut(s) 659, 673, 684, 847
MluNI TGGCCA 1 cut(s) 776
MlyI GAGTC 1 cut(s) 774
MmeI TCCRAC 2 cut(s) 221, 671
MnlI CCTC 4 cut(s) 32, 270, 613, 755
Mox20I TGGCCA 1 cut(s) 776
Mph1103I ATGCAT 1 cut(s) 475
MroI TCCGGA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 776
MseI TTAA 4 cut(s) 54, 150, 683, 846
MslI CAYNNNNRTG 1 cut(s) 503
Msp20I TGGCCA 1 cut(s) 776
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 1 cut(s) 554
MvaI CCWGG 1 cut(s) 554
MvnI CGCG 1 cut(s) 907
NdeII GATC 8 cut(s) 31, 109, 133, 229, 253, 409, 614, 632
NlaIII CATG 3 cut(s) 32, 743, 844
NlaIV GGNNCC 2 cut(s) 489, 697
NmuCI GTSAC 1 cut(s) 41
NsiI ATGCAT 1 cut(s) 475
NspV TTCGAA 1 cut(s) 861
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PfeI GAWTC 3 cut(s) 335, 800, 858
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 1 cut(s) 773
PpsI GAGTC 1 cut(s) 773
PshBI ATTAAT 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 552
PspGI CCWGG 1 cut(s) 552
PspN4I GGNNCC 2 cut(s) 489, 697
PsuI RGATCY 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 503
SaqAI TTAA 4 cut(s) 54, 150, 683, 846
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 8 cut(s) 31, 109, 133, 229, 253, 409, 614, 632
SchI GAGTC 1 cut(s) 774
ScrFI CCNGG 1 cut(s) 554
SfaNI GCATC 5 cut(s) 26, 76, 520, 611, 743
SfuI TTCGAA 1 cut(s) 861
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 717
SmoI CTYRAG 1 cut(s) 717
Sse9I AATT 4 cut(s) 659, 673, 684, 847
SsiI CCGC 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 552
StyI CCWWGG 3 cut(s) 423, 483, 690
TaaI ACNGT 2 cut(s) 211, 820
TaqI TCGA 4 cut(s) 96, 108, 416, 861
TaqII GACCGA 1 cut(s) 402
TasI AATT 4 cut(s) 659, 673, 684, 847
TfiI GAWTC 3 cut(s) 335, 800, 858
Tru1I TTAA 4 cut(s) 54, 150, 683, 846
Tru9I TTAA 4 cut(s) 54, 150, 683, 846
TscAI CASTG 1 cut(s) 823
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 5 cut(s) 94, 144, 314, 470, 728
TspRI CASTG 1 cut(s) 823
VspI ATTAAT 1 cut(s) 54
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.