Rroxscaffold_1G00023420

Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004109
Physical Location & Seq
Forward (+)
28911307 .. 28912856
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00023420.1

Sequence Viewer

Length: 897 bp
ATGGATGGGATGGGTGCATCTGGAAACCACGATCTAGGAGGTCACAGCATCATTAATGACTTTGAGTTACAAGACTTCATAGATGATGCAAACTTCGACCAGTTCATCAATCTTATTCGCGGCGAAAATGAGGATCCAGCAGCAGCTCATTTTGATTGCGACCTTATCAGTGGCTCGTGCTTTGTTGATAACCAGTTTAGTCCAACTACTCCGGGTAGTGGTTTCTATGGTTTAGATCATAATACTACTGTCGTATCAGATCCAGCTCCTTTTCTTACTACATTACCAAATTTTGATGGAGAAATGAACGATATTGGGGAAGAAGATAATGACGCAGACTCATGTGGAACAACGACAACAACCACCACAAATACCACAGCCACGACGACAAAGAAGCCAAAGGTTGATCGGTCGAGAACCTTGGTTTCGGAGAGGAAGAGGAGGGGAAGGATGAAAGAAAAACTCTATGCATTGAGGTCCTTGGTTCCTAACATCACTAAGATGGATAAAGCCTCCATCGTTGGAGATGCTGTATCATATGTACAAGATCTTCAAAAACACGCTAAGAAGCTGGAAGCTGAGATTTCAGGTCTTGAAGCATCCATAGAAGGATCAGGGATAGATGTATCCCAAGTAGAGGAGAAAAGCTTTTATGTGAAAGTGGGATGCAACAAAGGAGTAGGCGTGGCCACAGCGCTTTACAAGGCTATTGAATCCCTTACAAGCTTCAATGTTCTGAGTTCAAACTTGAACACGGTTAATTCTGGGAGATTCGAAATAACACTCAGTTTGAATGTGAAAGAAAGTGAGCAGGAGATCATCAACTTACCAAACCTGAAACTTTGGGTGACTGGAGCTTTCCTGAACCAAGGTTTTGAGCTAGCTACACGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

298

Amino Acids

32.44

Weight (kDa)

4.71

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 141 - 185 3.3e-10 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 120
AciI CCGC 1 cut(s) 120
AclWI GGATC 4 cut(s) 128, 141, 254, 619
AcoI YGGCCR 1 cut(s) 687
AcsI RAATTY 1 cut(s) 289
AfaI GTAC 1 cut(s) 543
AfeI AGCGCT 1 cut(s) 696
AfiI CCNNNNNNNGG 2 cut(s) 218, 637
AgsI TTSAA 7 cut(s) 554, 596, 713, 730, 744, 751, 793
AluBI AGCT 9 cut(s) 146, 266, 571, 578, 648, 726, 857, 880, 884
AluI AGCT 9 cut(s) 146, 266, 571, 578, 648, 726, 857, 880, 884
AlwI GGATC 4 cut(s) 128, 141, 254, 619
Aor51HI AGCGCT 1 cut(s) 696
AoxI GGCC 1 cut(s) 687
ApeKI GCWGC 2 cut(s) 140, 143
ApoI RAATTY 1 cut(s) 289
AseI ATTAAT 1 cut(s) 54
AspLEI GCGC 1 cut(s) 697
AspS9I GGNCC 1 cut(s) 477
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 1 cut(s) 859
AsuII TTCGAA 1 cut(s) 774
AsuNHI GCTAGC 1 cut(s) 880
AvaII GGWCC 1 cut(s) 477
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BalI TGGCCA 1 cut(s) 689
BamHI GGATCC 1 cut(s) 133
BarI GAAGNNNNNNTAC 2 cut(s) 534, 566
BauI CACGAG 1 cut(s) 175
BbvI GCAGC 2 cut(s) 152, 155
BccI CCATC 4 cut(s) 4, 290, 496, 524
BciVI GTATCC 1 cut(s) 637
BcnI CCSGG 1 cut(s) 213
BfaI CTAG 2 cut(s) 35, 881
BfoI RGCGCY 1 cut(s) 698
BfuI GTATCC 1 cut(s) 637
BglII AGATCT 1 cut(s) 547
BisI GCNGC 3 cut(s) 121, 141, 144
BlsI GCNGC 3 cut(s) 122, 142, 145
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 1 cut(s) 477
BmgT120I GGNCC 1 cut(s) 477
BmiI GGNNCC 2 cut(s) 135, 486
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 6 cut(s) 26, 57, 76, 517, 608, 656
BmtI GCTAGC 1 cut(s) 884
BpmI CTGGAG 1 cut(s) 873
Bpu14I TTCGAA 1 cut(s) 774
BpuMI CCSGG 1 cut(s) 213
BsaJI CCNNGG 3 cut(s) 420, 480, 868
BsaXI ACNNNNNCTCC 4 cut(s) 516, 546, 669, 699
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 637
Bse1I ACTGG 3 cut(s) 100, 193, 856
BseDI CCNNGG 3 cut(s) 420, 480, 868
BseGI GGATG 5 cut(s) 10, 15, 456, 599, 671
BseLI CCNNNNNNNGG 2 cut(s) 218, 637
BseMII CTCAG 3 cut(s) 570, 728, 799
BseNI ACTGG 3 cut(s) 100, 193, 856
BseRI GAGGAG 2 cut(s) 454, 653
BseXI GCAGC 2 cut(s) 152, 155
Bsh1236I CGCG 1 cut(s) 120
Bsh1285I CGRYCG 1 cut(s) 413
BshFI GGCC 1 cut(s) 689
BsiEI CGRYCG 1 cut(s) 413
BsiSI CCGG 1 cut(s) 212
BslI CCNNNNNNNGG 2 cut(s) 218, 637
BsnI GGCC 1 cut(s) 689
Bsp119I TTCGAA 1 cut(s) 774
Bsp1407I TGTACA 1 cut(s) 541
Bsp143I GATC 8 cut(s) 31, 133, 235, 259, 406, 547, 611, 816
BspACI CCGC 1 cut(s) 120
BspANI GGCC 1 cut(s) 689
BspCNI CTCAG 3 cut(s) 571, 729, 798
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 2 cut(s) 135, 486
BspOI GCTAGC 1 cut(s) 884
BspPI GGATC 4 cut(s) 128, 141, 254, 619
BspT104I TTCGAA 1 cut(s) 774
BsrGI TGTACA 1 cut(s) 541
BsrI ACTGG 3 cut(s) 100, 193, 856
BssECI CCNNGG 3 cut(s) 420, 480, 868
BssMI GATC 8 cut(s) 31, 133, 235, 259, 406, 547, 611, 816
BssSI CACGAG 1 cut(s) 175
BssT1I CCWWGG 3 cut(s) 420, 480, 868
Bst2BI CACGAG 1 cut(s) 175
Bst4CI ACNGT 2 cut(s) 250, 757
Bst6I CTCTTC 1 cut(s) 431
BstAUI TGTACA 1 cut(s) 541
BstBI TTCGAA 1 cut(s) 774
BstC8I GCNNGC 1 cut(s) 882
BstDEI CTNAG 5 cut(s) 498, 564, 579, 737, 785
BstF5I GGATG 5 cut(s) 10, 15, 456, 599, 671
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 698
BstHHI GCGC 1 cut(s) 697
BstKTI GATC 8 cut(s) 34, 136, 238, 262, 409, 550, 614, 819
BstMBI GATC 8 cut(s) 31, 133, 235, 259, 406, 547, 611, 816
BstMCI CGRYCG 1 cut(s) 413
BstSCI CCNGG 1 cut(s) 211
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 2 cut(s) 152, 155
BstX2I RGATCY 3 cut(s) 133, 259, 547
BstYI RGATCY 3 cut(s) 133, 259, 547
BsuI GTATCC 1 cut(s) 637
BsuRI GGCC 1 cut(s) 689
BtsCI GGATG 5 cut(s) 10, 15, 456, 599, 671
BtsIMutI CAGTG 1 cut(s) 175
Cac8I GCNNGC 1 cut(s) 882
CfoI GCGC 1 cut(s) 697
Cfr13I GGNCC 1 cut(s) 477
CseI GACGC 1 cut(s) 341
Csp6I GTAC 1 cut(s) 542
CviAII CATG 1 cut(s) 342
CviQI GTAC 1 cut(s) 542
DdeI CTNAG 5 cut(s) 498, 564, 579, 737, 785
DpnI GATC 8 cut(s) 33, 135, 237, 261, 408, 549, 613, 818
DpnII GATC 8 cut(s) 31, 133, 235, 259, 406, 547, 611, 816
EaeI YGGCCR 1 cut(s) 687
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
Eco130I CCWWGG 3 cut(s) 420, 480, 868
Eco47I GGWCC 1 cut(s) 477
Eco47III AGCGCT 1 cut(s) 696
EcoO109I RGGNCCY 1 cut(s) 477
EcoT14I CCWWGG 3 cut(s) 420, 480, 868
EcoT22I ATGCAT 1 cut(s) 472
ErhI CCWWGG 3 cut(s) 420, 480, 868
FaeI CATG 1 cut(s) 345
FaiI YATR 9 cut(s) 80, 228, 240, 343, 468, 538, 540, 605, 654
FatI CATG 1 cut(s) 341
FauNDI CATATG 1 cut(s) 538
Fnu4HI GCNGC 3 cut(s) 121, 141, 144
FokI GGATG 5 cut(s) 17, 22, 463, 586, 678
Fsp4HI GCNGC 3 cut(s) 121, 141, 144
FspBI CTAG 2 cut(s) 35, 881
GlaI GCGC 1 cut(s) 696
GluI GCNGC 3 cut(s) 121, 141, 144
GsuI CTGGAG 1 cut(s) 873
HaeII RGCGCY 1 cut(s) 698
HaeIII GGCC 1 cut(s) 689
HapII CCGG 1 cut(s) 212
HgaI GACGC 1 cut(s) 341
HhaI GCGC 1 cut(s) 697
Hin1II CATG 1 cut(s) 345
Hin6I GCGC 1 cut(s) 695
HinP1I GCGC 1 cut(s) 695
HindIII AAGCTT 2 cut(s) 646, 724
HinfI GANTC 3 cut(s) 338, 713, 771
HpaII CCGG 1 cut(s) 212
HphI GGTGA 1 cut(s) 859
Hpy188I TCNGA 3 cut(s) 259, 430, 738
Hpy188III TCNNGA 4 cut(s) 21, 414, 593, 862
Hpy99I CGWCG 1 cut(s) 388
HpyAV CCTTC 2 cut(s) 441, 602
HpyCH4III ACNGT 2 cut(s) 250, 757
HpyCH4V TGCA 4 cut(s) 17, 89, 470, 669
HpyF3I CTNAG 5 cut(s) 498, 564, 579, 737, 785
Hsp92II CATG 1 cut(s) 345
HspAI GCGC 1 cut(s) 695
Kzo9I GATC 8 cut(s) 31, 133, 235, 259, 406, 547, 611, 816
LmnI GCTCC 2 cut(s) 271, 854
Lsp1109I GCAGC 2 cut(s) 152, 155
LweI GCATC 6 cut(s) 26, 57, 76, 517, 608, 656
MaeI CTAG 2 cut(s) 35, 881
MaeIII GTNAC 3 cut(s) 41, 66, 847
MalI GATC 8 cut(s) 33, 135, 237, 261, 408, 549, 613, 818
MboI GATC 8 cut(s) 31, 133, 235, 259, 406, 547, 611, 816
MboII GAAGA 4 cut(s) 332, 335, 448, 542
MflI RGATCY 3 cut(s) 133, 259, 547
MlsI TGGCCA 1 cut(s) 689
MluCI AATT 2 cut(s) 289, 760
MluNI TGGCCA 1 cut(s) 689
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 2 cut(s) 227, 502
MnlI CCTC 8 cut(s) 32, 124, 426, 432, 435, 468, 523, 631
Mox20I TGGCCA 1 cut(s) 689
Mph1103I ATGCAT 1 cut(s) 472
MscI TGGCCA 1 cut(s) 689
MseI TTAA 2 cut(s) 54, 759
MslI CAYNNNNRTG 1 cut(s) 500
Msp20I TGGCCA 1 cut(s) 689
MspI CCGG 1 cut(s) 212
MspR9I CCNGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 120
NciI CCSGG 1 cut(s) 213
NdeI CATATG 1 cut(s) 538
NdeII GATC 8 cut(s) 31, 133, 235, 259, 406, 547, 611, 816
NheI GCTAGC 1 cut(s) 880
NlaIII CATG 1 cut(s) 345
NlaIV GGNNCC 2 cut(s) 135, 486
NmuCI GTSAC 2 cut(s) 41, 847
NsiI ATGCAT 1 cut(s) 472
NspV TTCGAA 1 cut(s) 774
PfeI GAWTC 2 cut(s) 713, 771
PkrI GCNGC 3 cut(s) 122, 142, 145
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
PpuMI RGGWCCY 1 cut(s) 477
PshBI ATTAAT 1 cut(s) 54
Psp5II RGGWCCY 1 cut(s) 477
PspN4I GGNNCC 2 cut(s) 135, 486
PspPI GGNCC 1 cut(s) 477
PspPPI RGGWCCY 1 cut(s) 477
PsuI RGATCY 3 cut(s) 133, 259, 547
RsaI GTAC 1 cut(s) 543
RsaNI GTAC 1 cut(s) 542
RseI CAYNNNNRTG 1 cut(s) 500
SaqAI TTAA 2 cut(s) 54, 759
SatI GCNGC 3 cut(s) 121, 141, 144
Sau3AI GATC 8 cut(s) 31, 133, 235, 259, 406, 547, 611, 816
Sau96I GGNCC 1 cut(s) 477
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 213
SfaNI GCATC 6 cut(s) 26, 57, 76, 517, 608, 656
SfuI TTCGAA 1 cut(s) 774
SinI GGWCC 1 cut(s) 477
SmiMI CAYNNNNRTG 1 cut(s) 500
Sse9I AATT 2 cut(s) 289, 760
SsiI CCGC 1 cut(s) 120
SspMI CTAG 2 cut(s) 35, 881
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 3 cut(s) 420, 480, 868
TaaI ACNGT 2 cut(s) 250, 757
TaqI TCGA 3 cut(s) 96, 413, 774
TaqII GACCGA 1 cut(s) 399
TasI AATT 2 cut(s) 289, 760
TatI WGTACW 1 cut(s) 541
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 2 cut(s) 713, 771
Tru1I TTAA 2 cut(s) 54, 759
Tru9I TTAA 2 cut(s) 54, 759
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 2 cut(s) 41, 847
TseI GCWGC 2 cut(s) 140, 143
Tsp45I GTSAC 2 cut(s) 41, 847
TspDTI ATGAA 4 cut(s) 67, 94, 320, 467
TspRI CASTG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 477
VspI ATTAAT 1 cut(s) 54
XapI RAATTY 1 cut(s) 289
XspI CTAG 2 cut(s) 35, 881
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.