Rw5G034980

Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004109
Physical Location & Seq
Reverse (-)
60393575 .. 60395132
1558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034980.1

Sequence Viewer

Length: 984 bp
ATGGATGGGATGGGTGCATCCGGAAACCATGATCTTAGAGGTCACAGCGTCATTAATGACTTTGAGTTACAAGATTTCATAGATGATGCAAACTTCGACCAGTTCATCGATCTTATTCGTGGAGAAAATGAAGATCCAGCAGCTCAGTTTAATCTTGACCTTACCAGCGGGTCATTCTTTGATAACCAACTTTGTCCAACTACTGATACGGTCAGTTTTTTTGGTTTTGATCATAATCTTACAATCATATGCGATCCACCTCCCTTTCTTACTGCATTACCGAGTTTTGGTGGAGAAATGAACGACATTGGCGAAGAAGATAATGATGGGGGAGATTCGTGTGGGACAACGACAACGACAACAACAACCACCACAACCACAGCCACAAAGACGAAGAAGCCAAAGGTTGATCGGTCGAGAACCTTGGTTTCGGAAAGAAGGAGAAGGGGAAGGATGAAGGAAAAACTCTACGCATTGCGTTCCTTGGTTCCTAACATCACTAAGATGGATAAGGCGTCCATTATTGGAGATGCTGTATTGTATGTGCTAGACCTGGAAAAGCAGGCTAAGAAGCTGAGAGCTGAGATTTTGGGACTTGAAGCATCCTTAGAAGGATCAGAGGAAAATCAAGGATCAACCCAAAATCCAACCAAGAATAAATTTGTTACAGACAATTACCATTTAATTCCCAAGGGAACCATTCAGATAGATGTGTCTCAAGTAAAGGAGAAAGGCTTTCATGTGAAAGTGGGATGCAACAGAGGAGTAGGGGTGGCCACAGCACTTTACAAGGCCATAGAATCCCTTACAAGCTTCACTGTTCAGAGTTCAAACTTGAACATGGTTAATTCCAAGAGATTCGAAATAACACTCGCCTTGAACGTAAAAGAGTGCGAGCACGAGATCATGAACTTACCAAATTTGAAACTTTGGGTGACTGGGGCTTTTCTGAACGAAGGTTTTGAATTAGCTACAATCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

327

Amino Acids

36.11

Weight (kDa)

5.05

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 142 - 186 2.4e-08 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 20
AciI CCGC 1 cut(s) 168
AclWI GGATC 4 cut(s) 128, 248, 622, 640
AcoI YGGCCR 1 cut(s) 774
AcsI RAATTY 2 cut(s) 659, 919
AcyI GRCGYC 1 cut(s) 515
AfiI CCNNNNNNNGG 1 cut(s) 287
AgsI TTSAA 6 cut(s) 599, 831, 838, 880, 925, 965
AjnI CCWGG 1 cut(s) 552
AluBI AGCT 5 cut(s) 143, 574, 581, 813, 971
AluI AGCT 5 cut(s) 143, 574, 581, 813, 971
Alw21I GWGCWC 1 cut(s) 900
Alw26I GTCTC 1 cut(s) 720
AlwI GGATC 4 cut(s) 128, 248, 622, 640
Aor13HI TCCGGA 1 cut(s) 20
AoxI GGCC 2 cut(s) 774, 792
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 2 cut(s) 659, 919
AseI ATTAAT 1 cut(s) 54
AsuHPI GGTGA 1 cut(s) 946
AsuII TTCGAA 1 cut(s) 861
BalI TGGCCA 1 cut(s) 776
BauI CACGAG 1 cut(s) 899
Bbv12I GWGCWC 1 cut(s) 900
BbvI GCAGC 1 cut(s) 152
BccI CCATC 3 cut(s) 4, 320, 499
BciT130I CCWGG 1 cut(s) 554
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 720
BfaI CTAG 1 cut(s) 548
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 1 cut(s) 554
BmiI GGNNCC 2 cut(s) 489, 697
BmrFI CCNGG 1 cut(s) 554
BmrI ACTGGG 1 cut(s) 948
BmsI GCATC 5 cut(s) 26, 76, 520, 611, 743
BmuI ACTGGG 1 cut(s) 948
Bpu14I TTCGAA 1 cut(s) 861
BpuEI CTTGAG 1 cut(s) 702
Bsa29I ATCGAT 1 cut(s) 108
BsaBI GATNNNNATC 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 515
BsaJI CCNNGG 3 cut(s) 423, 483, 690
BsaWI WCCGGW 1 cut(s) 20
BsaXI ACNNNNNCTCC 4 cut(s) 519, 549, 756, 786
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 100, 943
Bse3DI GCAATG 1 cut(s) 473
Bse8I GATNNNNATC 1 cut(s) 234
BseAI TCCGGA 1 cut(s) 20
BseBI CCWGG 1 cut(s) 554
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 3 cut(s) 423, 483, 690
BseGI GGATG 6 cut(s) 10, 15, 17, 459, 602, 758
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 1 cut(s) 473
BseMII CTCAG 3 cut(s) 158, 566, 573
BseNI ACTGG 2 cut(s) 100, 943
BseRI GAGGAG 1 cut(s) 777
BseXI GCAGC 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 416
BshFI GGCC 2 cut(s) 776, 794
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 416
BsiHKAI GWGCWC 1 cut(s) 900
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 2 cut(s) 358, 606
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 720
BsmFI GGGAC 2 cut(s) 358, 606
BsnI GGCC 2 cut(s) 776, 794
Bsp119I TTCGAA 1 cut(s) 861
Bsp1286I GDGCHC 1 cut(s) 900
Bsp13I TCCGGA 1 cut(s) 20
Bsp143I GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 903
BspACI CCGC 1 cut(s) 168
BspANI GGCC 2 cut(s) 776, 794
BspCNI CTCAG 3 cut(s) 157, 567, 574
BspDI ATCGAT 1 cut(s) 108
BspEI TCCGGA 1 cut(s) 20
BspHI TCATGA 1 cut(s) 906
BspLI GGNNCC 2 cut(s) 489, 697
BspPI GGATC 4 cut(s) 128, 248, 622, 640
BspT104I TTCGAA 1 cut(s) 861
BsrDI GCAATG 1 cut(s) 473
BsrI ACTGG 2 cut(s) 100, 943
BssECI CCNNGG 3 cut(s) 423, 483, 690
BssMI GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 903
BssNI GRCGYC 1 cut(s) 515
BssSI CACGAG 1 cut(s) 899
BssT1I CCWWGG 3 cut(s) 423, 483, 690
Bst2BI CACGAG 1 cut(s) 899
Bst2UI CCWGG 1 cut(s) 554
Bst4CI ACNGT 2 cut(s) 211, 820
BstACI GRCGYC 1 cut(s) 515
BstBI TTCGAA 1 cut(s) 861
BstC8I GCNNGC 2 cut(s) 564, 896
BstDEI CTNAG 7 cut(s) 35, 144, 501, 567, 575, 582, 607
BstF5I GGATG 6 cut(s) 10, 15, 17, 459, 602, 758
BstKTI GATC 9 cut(s) 34, 112, 136, 232, 256, 412, 617, 635, 906
BstMAI GTCTC 1 cut(s) 720
BstMBI GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 903
BstMCI CGRYCG 1 cut(s) 416
BstNI CCWGG 1 cut(s) 554
BstSCI CCNGG 1 cut(s) 552
BstV1I GCAGC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
Bsu15I ATCGAT 1 cut(s) 108
BsuRI GGCC 2 cut(s) 776, 794
BsuTUI ATCGAT 1 cut(s) 108
BtsCI GGATG 6 cut(s) 10, 15, 17, 459, 602, 758
BtsIMutI CAGTG 1 cut(s) 816
Cac8I GCNNGC 2 cut(s) 564, 896
CciI TCATGA 1 cut(s) 906
ClaI ATCGAT 1 cut(s) 108
CseI GACGC 2 cut(s) 37, 504
CviAII CATG 4 cut(s) 29, 740, 841, 907
DdeI CTNAG 7 cut(s) 35, 144, 501, 567, 575, 582, 607
DpnI GATC 9 cut(s) 33, 111, 135, 231, 255, 411, 616, 634, 905
DpnII GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 903
EaeI YGGCCR 1 cut(s) 774
Eco130I CCWWGG 3 cut(s) 423, 483, 690
EcoRII CCWGG 1 cut(s) 552
EcoT14I CCWWGG 3 cut(s) 423, 483, 690
ErhI CCWWGG 3 cut(s) 423, 483, 690
FaeI CATG 4 cut(s) 32, 743, 844, 910
FaqI GGGAC 2 cut(s) 358, 606
FatI CATG 4 cut(s) 28, 739, 840, 906
FauI CCCGC 1 cut(s) 161
FauNDI CATATG 1 cut(s) 248
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 6 cut(s) 4, 17, 22, 466, 589, 765
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 1 cut(s) 548
GluI GCNGC 1 cut(s) 141
HaeIII GGCC 2 cut(s) 776, 794
HapII CCGG 1 cut(s) 21
HgaI GACGC 2 cut(s) 37, 504
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 4 cut(s) 32, 743, 844, 910
HindIII AAGCTT 1 cut(s) 811
HinfI GANTC 3 cut(s) 335, 800, 858
HpaII CCGG 1 cut(s) 21
HphI GGTGA 1 cut(s) 946
Hpy188I TCNGA 5 cut(s) 433, 619, 705, 825, 951
Hpy188III TCNNGA 4 cut(s) 21, 155, 417, 907
HpyAV CCTTC 6 cut(s) 432, 438, 444, 451, 605, 950
HpyCH4III ACNGT 2 cut(s) 211, 820
HpyCH4IV ACGT 1 cut(s) 882
HpyCH4V TGCA 4 cut(s) 17, 89, 275, 756
HpyF3I CTNAG 7 cut(s) 35, 144, 501, 567, 575, 582, 607
HpySE526I ACGT 1 cut(s) 882
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 4 cut(s) 32, 743, 844, 910
Kpn2I TCCGGA 1 cut(s) 20
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 903
LpnPI CCDG 8 cut(s) 34, 113, 150, 178, 539, 548, 566, 924
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 5 cut(s) 26, 76, 520, 611, 743
MaeI CTAG 1 cut(s) 548
MaeII ACGT 1 cut(s) 882
MaeIII GTNAC 4 cut(s) 41, 66, 664, 934
MalI GATC 9 cut(s) 33, 111, 135, 231, 255, 411, 616, 634, 905
MboI GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 903
MboII GAAGA 4 cut(s) 143, 326, 329, 406
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 900
MlsI TGGCCA 1 cut(s) 776
MluCI AATT 6 cut(s) 659, 673, 684, 847, 919, 965
MluNI TGGCCA 1 cut(s) 776
MmeI TCCRAC 2 cut(s) 221, 671
MnlI CCTC 4 cut(s) 32, 270, 613, 755
Mox20I TGGCCA 1 cut(s) 776
MroI TCCGGA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 776
MseI TTAA 4 cut(s) 54, 150, 683, 846
MslI CAYNNNNRTG 1 cut(s) 503
Msp20I TGGCCA 1 cut(s) 776
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 1 cut(s) 554
MvaI CCWGG 1 cut(s) 554
NdeI CATATG 1 cut(s) 248
NdeII GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 903
NlaIII CATG 4 cut(s) 32, 743, 844, 910
NlaIV GGNNCC 2 cut(s) 489, 697
NmuCI GTSAC 2 cut(s) 41, 934
NspV TTCGAA 1 cut(s) 861
PagI TCATGA 1 cut(s) 906
PcsI WCGNNNNNNNCGW 2 cut(s) 309, 879
PfeI GAWTC 3 cut(s) 335, 800, 858
PkrI GCNGC 1 cut(s) 142
PshBI ATTAAT 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 552
PspGI CCWGG 1 cut(s) 552
PspN4I GGNNCC 2 cut(s) 489, 697
PsuI RGATCY 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 503
SaqAI TTAA 4 cut(s) 54, 150, 683, 846
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 903
ScrFI CCNGG 1 cut(s) 554
SduI GDGCHC 1 cut(s) 900
SfaNI GCATC 5 cut(s) 26, 76, 520, 611, 743
SfuI TTCGAA 1 cut(s) 861
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 717
SmoI CTYRAG 1 cut(s) 717
Sse9I AATT 6 cut(s) 659, 673, 684, 847, 919, 965
SsiI CCGC 1 cut(s) 168
SspMI CTAG 1 cut(s) 548
StyD4I CCNGG 1 cut(s) 552
StyI CCWWGG 3 cut(s) 423, 483, 690
TaaI ACNGT 2 cut(s) 211, 820
TaiI ACGT 1 cut(s) 885
TaqI TCGA 4 cut(s) 96, 108, 416, 861
TaqII GACCGA 1 cut(s) 402
TasI AATT 6 cut(s) 659, 673, 684, 847, 919, 965
TfiI GAWTC 3 cut(s) 335, 800, 858
Tru1I TTAA 4 cut(s) 54, 150, 683, 846
Tru9I TTAA 4 cut(s) 54, 150, 683, 846
TscAI CASTG 1 cut(s) 823
TseFI GTSAC 2 cut(s) 41, 934
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 2 cut(s) 41, 934
TspDTI ATGAA 7 cut(s) 67, 94, 144, 314, 470, 728, 923
TspRI CASTG 1 cut(s) 823
VspI ATTAAT 1 cut(s) 54
XapI RAATTY 2 cut(s) 659, 919
XspI CTAG 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.