RLG00000035133

Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004109
Physical Location & Seq
Reverse (-)
61365883 .. 61367546
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035133

Sequence Viewer

Length: 987 bp
ATGGATGGGATGGGTGCATCCGGAAACCATGATCTTAGAGGTCACAGCGTCATTAATGACTTTGAGTTACAAGACTTCATAGATGATGCAAACTTCGACCAGTTCATCGATCTTATTCGTGGAGAAAATGAAGATCCAGCAGCTCAGTTTAATCTTGACCTTACCAGCGGGTCATTCTTTGATAACCAACTTTGTCCAACTAATGATACGGTCAGTTTTTTTGGTTTTGATCATAATATTACAATCCTATGCGATCCAGCTCCCTTTCTTACTACATTACCGAGTTTTGGCGGAGAATTGAACGACATTGGCGAAGAAGATAATGATGGGGGGGATTCGTGTGGGACAACGACAACGACAACAACAACCACCACAACCACAGCCACAACGACGAAGAAGCCAACGGTTGATCGGTCGAGAACCTTGGTTTCGGAAAGAAGGAGGAGGGGAAGGATGAAGGAAAAACTCTATGCATTGCGGTCCTTGGTTCCTAACATCACTAAGATGGATAAGGCGTCCATTATTGGAGATGCTGTATTGTATGTGCAAGACCTGGAAAAGCAGGCTAAGAAGCTGAAAGCTGAGATTTTGGGACTTGAAGCATCCTTAGAAGGATCAGAGGAAAATCAAGGATCAACCCAAAATCCAACGACCAAGAATAAATTAGTTACAGACAATTACCATTTAATTCCCAAGGGAACCATTCAGATAGATGTGTCTCAAGTAAAGGAGAAAGGCTTTTATGTGAAAGTGGGATGCAACAGAGGAGTAGGGGTGGCCACAGCACTGTACAAGGCCATAGAATCCCTTACAAGCTTCACTGTTCAGAGTTCAAACTTGAACATGGTTAATTCCAAGAGATTCGAAATAACACTCGCTTTGAACGTAAAAGAGTGCGAGCACGAGATCATGAACTTACCAAATTTGAAACTTTGGTTGACTCGGGCTCTTCTGAACCAGGGTTTTGAATTAGCTACAGGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

329

Amino Acids

36.15

Weight (kDa)

4.97

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 142 - 186 6.6e-09 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 20
AciI CCGC 3 cut(s) 168, 291, 478
AclWI GGATC 4 cut(s) 128, 248, 622, 640
AcoI YGGCCR 1 cut(s) 777
AcsI RAATTY 1 cut(s) 922
AcyI GRCGYC 1 cut(s) 515
AfaI GTAC 1 cut(s) 791
AfiI CCNNNNNNNGG 1 cut(s) 287
AgsI TTSAA 7 cut(s) 301, 599, 834, 841, 883, 928, 968
AjnI CCWGG 2 cut(s) 552, 957
AluBI AGCT 6 cut(s) 143, 260, 574, 581, 816, 974
AluI AGCT 6 cut(s) 143, 260, 574, 581, 816, 974
Alw21I GWGCWC 1 cut(s) 903
Alw26I GTCTC 1 cut(s) 723
AlwI GGATC 4 cut(s) 128, 248, 622, 640
Ama87I CYCGRG 1 cut(s) 942
Aor13HI TCCGGA 1 cut(s) 20
AoxI GGCC 2 cut(s) 777, 795
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 1 cut(s) 922
AseI ATTAAT 1 cut(s) 54
AspS9I GGNCC 1 cut(s) 480
AsuII TTCGAA 1 cut(s) 864
AvaI CYCGRG 1 cut(s) 942
AvaII GGWCC 1 cut(s) 480
BalI TGGCCA 1 cut(s) 779
BanII GRGCYC 1 cut(s) 949
BauI CACGAG 1 cut(s) 902
Bbv12I GWGCWC 1 cut(s) 903
BbvI GCAGC 1 cut(s) 152
BccI CCATC 3 cut(s) 4, 320, 499
BciT130I CCWGG 2 cut(s) 554, 959
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 723
BfmI CTRYAG 1 cut(s) 975
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 2 cut(s) 554, 959
Bme18I GGWCC 1 cut(s) 480
BmeT110I CYCGRG 1 cut(s) 942
BmgT120I GGNCC 1 cut(s) 480
BmiI GGNNCC 2 cut(s) 489, 700
BmrFI CCNGG 2 cut(s) 554, 959
BmsI GCATC 5 cut(s) 26, 76, 520, 611, 746
Bpu14I TTCGAA 1 cut(s) 864
BpuEI CTTGAG 1 cut(s) 705
Bsa29I ATCGAT 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 515
BsaJI CCNNGG 4 cut(s) 423, 483, 693, 958
BsaWI WCCGGW 1 cut(s) 20
BsaXI ACNNNNNCTCC 4 cut(s) 519, 549, 759, 789
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 100
Bse3DI GCAATG 1 cut(s) 473
BseAI TCCGGA 1 cut(s) 20
BseBI CCWGG 2 cut(s) 554, 959
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 4 cut(s) 423, 483, 693, 958
BseGI GGATG 6 cut(s) 10, 15, 17, 459, 602, 761
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 1 cut(s) 473
BseMII CTCAG 2 cut(s) 158, 573
BseNI ACTGG 1 cut(s) 100
BseRI GAGGAG 2 cut(s) 457, 780
BseXI GCAGC 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 416
BshFI GGCC 2 cut(s) 779, 797
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 416
BsiHKAI GWGCWC 1 cut(s) 903
BsiHKCI CYCGRG 1 cut(s) 942
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 2 cut(s) 358, 606
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 723
BsmFI GGGAC 2 cut(s) 358, 606
BsnI GGCC 2 cut(s) 779, 797
BsoBI CYCGRG 1 cut(s) 942
Bsp119I TTCGAA 1 cut(s) 864
Bsp1286I GDGCHC 2 cut(s) 903, 949
Bsp13I TCCGGA 1 cut(s) 20
Bsp1407I TGTACA 1 cut(s) 789
Bsp143I GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 906
BspACI CCGC 3 cut(s) 168, 291, 478
BspANI GGCC 2 cut(s) 779, 797
BspCNI CTCAG 2 cut(s) 157, 574
BspDI ATCGAT 1 cut(s) 108
BspEI TCCGGA 1 cut(s) 20
BspHI TCATGA 1 cut(s) 909
BspLI GGNNCC 2 cut(s) 489, 700
BspPI GGATC 4 cut(s) 128, 248, 622, 640
BspQI GCTCTTC 1 cut(s) 954
BspT104I TTCGAA 1 cut(s) 864
BsrDI GCAATG 1 cut(s) 473
BsrGI TGTACA 1 cut(s) 789
BsrI ACTGG 1 cut(s) 100
BssECI CCNNGG 4 cut(s) 423, 483, 693, 958
BssMI GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 906
BssNI GRCGYC 1 cut(s) 515
BssSI CACGAG 1 cut(s) 902
BssT1I CCWWGG 3 cut(s) 423, 483, 693
Bst2BI CACGAG 1 cut(s) 902
Bst2UI CCWGG 2 cut(s) 554, 959
Bst4CI ACNGT 4 cut(s) 211, 406, 789, 823
Bst6I CTCTTC 1 cut(s) 954
BstACI GRCGYC 1 cut(s) 515
BstAUI TGTACA 1 cut(s) 789
BstBI TTCGAA 1 cut(s) 864
BstC8I GCNNGC 2 cut(s) 564, 899
BstDEI CTNAG 6 cut(s) 35, 144, 501, 567, 582, 607
BstF5I GGATG 6 cut(s) 10, 15, 17, 459, 602, 761
BstKTI GATC 9 cut(s) 34, 112, 136, 232, 256, 412, 617, 635, 909
BstMAI GTCTC 1 cut(s) 723
BstMBI GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 906
BstMCI CGRYCG 1 cut(s) 416
BstNI CCWGG 2 cut(s) 554, 959
BstSCI CCNGG 2 cut(s) 552, 957
BstSFI CTRYAG 1 cut(s) 975
BstV1I GCAGC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
Bsu15I ATCGAT 1 cut(s) 108
BsuRI GGCC 2 cut(s) 779, 797
BsuTUI ATCGAT 1 cut(s) 108
BtsCI GGATG 6 cut(s) 10, 15, 17, 459, 602, 761
BtsIMutI CAGTG 2 cut(s) 785, 819
Cac8I GCNNGC 2 cut(s) 564, 899
CciI TCATGA 1 cut(s) 909
Cfr13I GGNCC 1 cut(s) 480
ClaI ATCGAT 1 cut(s) 108
CseI GACGC 2 cut(s) 37, 504
Csp6I GTAC 1 cut(s) 790
CviAII CATG 3 cut(s) 29, 844, 910
CviQI GTAC 1 cut(s) 790
DdeI CTNAG 6 cut(s) 35, 144, 501, 567, 582, 607
DpnI GATC 9 cut(s) 33, 111, 135, 231, 255, 411, 616, 634, 908
DpnII GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 906
EaeI YGGCCR 1 cut(s) 777
Eam1104I CTCTTC 1 cut(s) 954
EarI CTCTTC 1 cut(s) 954
EciI GGCGGA 1 cut(s) 306
Eco130I CCWWGG 3 cut(s) 423, 483, 693
Eco24I GRGCYC 1 cut(s) 949
Eco47I GGWCC 1 cut(s) 480
Eco88I CYCGRG 1 cut(s) 942
EcoRII CCWGG 2 cut(s) 552, 957
EcoT14I CCWWGG 3 cut(s) 423, 483, 693
EcoT22I ATGCAT 1 cut(s) 475
EcoT38I GRGCYC 1 cut(s) 949
ErhI CCWWGG 3 cut(s) 423, 483, 693
FaeI CATG 3 cut(s) 32, 847, 913
FaqI GGGAC 2 cut(s) 358, 606
FatI CATG 3 cut(s) 28, 843, 909
FauI CCCGC 1 cut(s) 161
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 6 cut(s) 4, 17, 22, 466, 589, 768
FriOI GRGCYC 1 cut(s) 949
Fsp4HI GCNGC 1 cut(s) 141
GluI GCNGC 1 cut(s) 141
HaeIII GGCC 2 cut(s) 779, 797
HapII CCGG 1 cut(s) 21
HgaI GACGC 2 cut(s) 37, 504
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 3 cut(s) 32, 847, 913
HincII GTYRAC 1 cut(s) 939
HindII GTYRAC 1 cut(s) 939
HindIII AAGCTT 1 cut(s) 814
HinfI GANTC 4 cut(s) 335, 803, 861, 940
HpaII CCGG 1 cut(s) 21
Hpy166II GTNNAC 1 cut(s) 939
Hpy188I TCNGA 5 cut(s) 433, 619, 708, 828, 954
Hpy188III TCNNGA 4 cut(s) 21, 155, 417, 910
Hpy8I GTNNAC 1 cut(s) 939
Hpy99I CGWCG 1 cut(s) 394
HpyAV CCTTC 4 cut(s) 432, 444, 451, 605
HpyCH4III ACNGT 4 cut(s) 211, 406, 789, 823
HpyCH4IV ACGT 1 cut(s) 885
HpyCH4V TGCA 5 cut(s) 17, 89, 473, 547, 759
HpyF3I CTNAG 6 cut(s) 35, 144, 501, 567, 582, 607
HpySE526I ACGT 1 cut(s) 885
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 3 cut(s) 32, 847, 913
Kpn2I TCCGGA 1 cut(s) 20
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 906
LguI GCTCTTC 1 cut(s) 954
LmnI GCTCC 1 cut(s) 265
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 5 cut(s) 26, 76, 520, 611, 746
MaeII ACGT 1 cut(s) 885
MaeIII GTNAC 3 cut(s) 41, 66, 667
MalI GATC 9 cut(s) 33, 111, 135, 231, 255, 411, 616, 634, 908
MboI GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 906
MboII GAAGA 5 cut(s) 143, 326, 329, 406, 941
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 2 cut(s) 903, 949
MlsI TGGCCA 1 cut(s) 779
MluCI AATT 7 cut(s) 296, 662, 676, 687, 850, 922, 968
MluNI TGGCCA 1 cut(s) 779
MlyI GAGTC 1 cut(s) 934
MmeI TCCRAC 2 cut(s) 221, 671
MnlI CCTC 5 cut(s) 32, 435, 438, 613, 758
Mox20I TGGCCA 1 cut(s) 779
Mph1103I ATGCAT 1 cut(s) 475
MroI TCCGGA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 779
MseI TTAA 4 cut(s) 54, 150, 686, 849
MslI CAYNNNNRTG 1 cut(s) 503
Msp20I TGGCCA 1 cut(s) 779
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 2 cut(s) 554, 959
MvaI CCWGG 2 cut(s) 554, 959
NdeII GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 906
NlaIII CATG 3 cut(s) 32, 847, 913
NlaIV GGNNCC 2 cut(s) 489, 700
NmuCI GTSAC 1 cut(s) 41
NsiI ATGCAT 1 cut(s) 475
NspV TTCGAA 1 cut(s) 864
PagI TCATGA 1 cut(s) 909
PciSI GCTCTTC 1 cut(s) 954
PcsI WCGNNNNNNNCGW 2 cut(s) 309, 882
PfeI GAWTC 3 cut(s) 335, 803, 861
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 1 cut(s) 934
PpsI GAGTC 1 cut(s) 934
PshBI ATTAAT 1 cut(s) 54
Psp6I CCWGG 2 cut(s) 552, 957
PspGI CCWGG 2 cut(s) 552, 957
PspN4I GGNNCC 2 cut(s) 489, 700
PspPI GGNCC 1 cut(s) 480
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 1 cut(s) 791
RsaNI GTAC 1 cut(s) 790
RseI CAYNNNNRTG 1 cut(s) 503
SapI GCTCTTC 1 cut(s) 954
SaqAI TTAA 4 cut(s) 54, 150, 686, 849
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 9 cut(s) 31, 109, 133, 229, 253, 409, 614, 632, 906
Sau96I GGNCC 1 cut(s) 480
SchI GAGTC 1 cut(s) 934
ScrFI CCNGG 2 cut(s) 554, 959
SduI GDGCHC 2 cut(s) 903, 949
SfaNI GCATC 5 cut(s) 26, 76, 520, 611, 746
SfcI CTRYAG 1 cut(s) 975
SfuI TTCGAA 1 cut(s) 864
SinI GGWCC 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 720
SmoI CTYRAG 1 cut(s) 720
Sse9I AATT 7 cut(s) 296, 662, 676, 687, 850, 922, 968
SsiI CCGC 3 cut(s) 168, 291, 478
SspI AATATT 1 cut(s) 238
StyD4I CCNGG 2 cut(s) 552, 957
StyI CCWWGG 3 cut(s) 423, 483, 693
TaaI ACNGT 4 cut(s) 211, 406, 789, 823
TaiI ACGT 1 cut(s) 888
TaqI TCGA 4 cut(s) 96, 108, 416, 864
TaqII GACCGA 1 cut(s) 402
TasI AATT 7 cut(s) 296, 662, 676, 687, 850, 922, 968
TatI WGTACW 1 cut(s) 789
TfiI GAWTC 3 cut(s) 335, 803, 861
Tru1I TTAA 4 cut(s) 54, 150, 686, 849
Tru9I TTAA 4 cut(s) 54, 150, 686, 849
TscAI CASTG 2 cut(s) 792, 826
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 5 cut(s) 67, 94, 144, 470, 926
TspRI CASTG 2 cut(s) 792, 826
VpaK11BI GGWCC 1 cut(s) 480
VspI ATTAAT 1 cut(s) 54
XapI RAATTY 1 cut(s) 922
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.