MD03G1219900.v1.1

Organic solute transporter Ostalpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004499
Physical Location & Seq
Reverse (-)
30432264 .. 30434982
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1219900.v1.1.491

Sequence Viewer

Length: 876 bp
ATGGCAAATCCTGCACAACTTGTTCTTCTGGGTTCCACTTTCTGTATGATGGTTACAACGCATTTCTCACTGCAGCTTCTCTCAGAGCATTTTTTCTGCTGGAATAAGCCAAAGGAGCAAAAGGCCATCGTAATCATCATACTTATGGCCCCCTTATATGCCATTGATTCCTTTGTGGGCTTGTTGGATTACCAGGGAAGCAAAGTTTCGTTCACGGTCTTGGACTCGATCAAAGAATGCTACGAGGCTTTGGTAATAGCCAAGTTTTTGGCTTTGCTGTACAGCTACTTGAACATATCCATAAGTAAAAACATCGTGCCAGATGAAATCAAAGGACGGGAGATTCACCATTCATTTCCAATGACTCTTTTTATGCCTCGCACTGTTCGTCTGAACCATCATACTTTGAAGCTTCTCAAGTACTGGACATGGCAATTTGTTGTGATTCGCCCTGTGTGCTCCATCTTGATGATAACTCTTCAACTTCTTGGTGTATATCCCAGCTGGGTTAGCTGGACATTCACTATAATTCTGAACATTTCAGTGTCACTGGCTTTGTATTCTCTCATCGCCTTCTACCATGTGTTTGCTAAAGAGCTGGCACCACACAAACCGCTCACTAAATTCTTGTGCATCAAAGGGATCGTCTTCTTCTGCTTTTGGCAGGGGATTGTCCTTGATATTCTCGCAGCGCTGAAAATAATCCGATCTCATCATATCTGGCTAGACGTGGAGCATATTGAGGAAGCTCTTCAAAACATACTAGTGTGCGTGGAGATGGTTTTCTTTTCAGTTGTTCAGAAGTATGCATACAGTGCCGAGCCATACAGAGATGCTGAAATATCGACTAAGGCATATGATAGGAAGAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Solute_trans_a

Protein Analysis

292

Amino Acids

33.64

Weight (kDa)

8.57

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 10 - 276 1.1e-76 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 601
AccBSI CCGCTC 1 cut(s) 616
AciI CCGC 1 cut(s) 614
AclWI GGATC 1 cut(s) 650
AcsI RAATTY 1 cut(s) 623
AfaI GTAC 2 cut(s) 281, 422
AfeI AGCGCT 1 cut(s) 693
AgsI TTSAA 4 cut(s) 292, 409, 482, 755
AhlI ACTAGT 1 cut(s) 763
AjiI CACGTC 1 cut(s) 730
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 7 cut(s) 76, 285, 412, 504, 513, 598, 749
AluI AGCT 7 cut(s) 76, 285, 412, 504, 513, 598, 749
Alw21I GWGCWC 1 cut(s) 461
AlwI GGATC 1 cut(s) 650
Aor51HI AGCGCT 1 cut(s) 693
AoxI GGCC 2 cut(s) 123, 147
ApeKI GCWGC 2 cut(s) 73, 689
ApoI RAATTY 1 cut(s) 623
ArsI GACNNNNNNTTYG 2 cut(s) 630, 662
Asp700I GAANNNNTTC 1 cut(s) 750
AspLEI GCGC 1 cut(s) 694
AspS9I GGNCC 1 cut(s) 148
AsuHPI GGTGA 1 cut(s) 338
BanI GGYRCC 1 cut(s) 601
BarI GAAGNNNNNNTAC 2 cut(s) 794, 826
BbsI GAAGAC 1 cut(s) 640
Bbv12I GWGCWC 1 cut(s) 461
BbvI GCAGC 2 cut(s) 85, 701
BccI CCATC 5 cut(s) 43, 134, 405, 470, 772
BciT130I CCWGG 1 cut(s) 194
BcuI ACTAGT 1 cut(s) 763
BfaI CTAG 2 cut(s) 725, 764
BfmI CTRYAG 1 cut(s) 71
BfoI RGCGCY 1 cut(s) 695
BisI GCNGC 2 cut(s) 74, 690
BlsI GCNGC 2 cut(s) 75, 691
BmcAI AGTACT 1 cut(s) 422
Bme1390I CCNGG 1 cut(s) 194
BmgBI CACGTC 1 cut(s) 730
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 3 cut(s) 34, 150, 603
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 2 cut(s) 642, 823
BpiI GAAGAC 1 cut(s) 640
BpuEI CTTGAG 1 cut(s) 401
BsaJI CCNNGG 1 cut(s) 193
Bse1I ACTGG 2 cut(s) 428, 555
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 1 cut(s) 193
BseMII CTCAG 1 cut(s) 96
BseNI ACTGG 2 cut(s) 428, 555
BseXI GCAGC 2 cut(s) 85, 701
BseYI CCCAGC 2 cut(s) 500, 504
BshFI GGCC 2 cut(s) 125, 149
BshNI GGYRCC 1 cut(s) 601
BsiHKAI GWGCWC 1 cut(s) 461
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 2 cut(s) 125, 149
Bsp1286I GDGCHC 1 cut(s) 461
Bsp1407I TGTACA 1 cut(s) 279
Bsp143I GATC 3 cut(s) 228, 642, 707
BspACI CCGC 1 cut(s) 614
BspANI GGCC 2 cut(s) 125, 149
BspCNI CTCAG 1 cut(s) 95
BspLI GGNNCC 3 cut(s) 34, 150, 603
BspMAI CTGCAG 1 cut(s) 75
BspPI GGATC 1 cut(s) 650
BspQI GCTCTTC 1 cut(s) 756
BspT107I GGYRCC 1 cut(s) 601
BsrBI CCGCTC 1 cut(s) 616
BsrGI TGTACA 1 cut(s) 279
BsrI ACTGG 2 cut(s) 428, 555
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 3 cut(s) 228, 642, 707
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 3 cut(s) 217, 385, 815
Bst6I CTCTTC 2 cut(s) 483, 756
BstAPI GCANNNNNTGC 2 cut(s) 11, 815
BstAUI TGTACA 1 cut(s) 279
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 2 cut(s) 82, 849
BstH2I RGCGCY 1 cut(s) 695
BstHHI GCGC 1 cut(s) 694
BstKTI GATC 3 cut(s) 231, 645, 710
BstMBI GATC 3 cut(s) 228, 642, 707
BstMWI GCNNNNNNNGC 5 cut(s) 11, 115, 456, 510, 815
BstNI CCWGG 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 192
BstSFI CTRYAG 1 cut(s) 71
BstV1I GCAGC 2 cut(s) 85, 701
BstV2I GAAGAC 1 cut(s) 640
BstXI CCANNNNNNTGG 1 cut(s) 268
BsuRI GGCC 2 cut(s) 125, 149
BtgZI GCGATG 1 cut(s) 553
BtrI CACGTC 1 cut(s) 730
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 5 cut(s) 68, 381, 548, 549, 820
Cac8I GCNNGC 1 cut(s) 600
CfoI GCGC 1 cut(s) 694
Cfr13I GGNCC 1 cut(s) 148
Csp6I GTAC 2 cut(s) 280, 421
CviAII CATG 2 cut(s) 429, 581
CviQI GTAC 2 cut(s) 280, 421
DdeI CTNAG 2 cut(s) 82, 849
DpnI GATC 3 cut(s) 230, 644, 709
DpnII GATC 3 cut(s) 228, 642, 707
Eam1104I CTCTTC 2 cut(s) 483, 756
EarI CTCTTC 2 cut(s) 483, 756
Eco47III AGCGCT 1 cut(s) 693
EcoRII CCWGG 1 cut(s) 192
EcoT22I ATGCAT 1 cut(s) 811
FaeI CATG 2 cut(s) 432, 584
FatI CATG 2 cut(s) 428, 580
FauNDI CATATG 1 cut(s) 856
Fnu4HI GCNGC 2 cut(s) 74, 690
Fsp4HI GCNGC 2 cut(s) 74, 690
FspBI CTAG 2 cut(s) 725, 764
GlaI GCGC 1 cut(s) 693
GluI GCNGC 2 cut(s) 74, 690
GsaI CCCAGC 2 cut(s) 504, 508
HaeII RGCGCY 1 cut(s) 695
HaeIII GGCC 2 cut(s) 125, 149
HhaI GCGC 1 cut(s) 694
Hin1II CATG 2 cut(s) 432, 584
Hin6I GCGC 1 cut(s) 692
HinP1I GCGC 1 cut(s) 692
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 5 cut(s) 167, 224, 343, 364, 445
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 1 cut(s) 213
Hpy188I TCNGA 5 cut(s) 85, 393, 534, 707, 801
Hpy188III TCNNGA 1 cut(s) 466
Hpy8I GTNNAC 1 cut(s) 213
HpyAV CCTTC 1 cut(s) 583
HpyCH4III ACNGT 3 cut(s) 217, 385, 815
HpyCH4IV ACGT 1 cut(s) 729
HpyCH4V TGCA 4 cut(s) 14, 73, 633, 809
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 115, 456, 510, 815
HpyF3I CTNAG 2 cut(s) 82, 849
HpySE526I ACGT 1 cut(s) 729
Hsp92II CATG 2 cut(s) 432, 584
HspAI GCGC 1 cut(s) 692
Kzo9I GATC 3 cut(s) 228, 642, 707
LguI GCTCTTC 1 cut(s) 756
LmnI GCTCC 3 cut(s) 115, 464, 733
Lsp1109I GCAGC 2 cut(s) 85, 701
LweI GCATC 2 cut(s) 642, 823
MaeI CTAG 2 cut(s) 725, 764
MaeII ACGT 1 cut(s) 729
MaeIII GTNAC 2 cut(s) 52, 546
MalI GATC 3 cut(s) 230, 644, 709
MbiI CCGCTC 1 cut(s) 616
MboI GATC 3 cut(s) 228, 642, 707
MboII GAAGA 5 cut(s) 17, 470, 640, 643, 743
MhlI GDGCHC 1 cut(s) 461
MluCI AATT 3 cut(s) 434, 528, 623
MlyI GAGTC 2 cut(s) 218, 358
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 3 cut(s) 238, 387, 736
Mph1103I ATGCAT 1 cut(s) 811
MroXI GAANNNNTTC 1 cut(s) 750
MslI CAYNNNNRTG 4 cut(s) 143, 467, 542, 764
MspA1I CMGCKG 1 cut(s) 504
MspR9I CCNGG 1 cut(s) 194
Mva1269I GAATGC 1 cut(s) 242
MvaI CCWGG 1 cut(s) 194
MwoI GCNNNNNNNGC 5 cut(s) 11, 115, 456, 510, 815
NdeI CATATG 1 cut(s) 856
NdeII GATC 3 cut(s) 228, 642, 707
NlaIII CATG 2 cut(s) 432, 584
NlaIV GGNNCC 3 cut(s) 34, 150, 603
NmeAIII GCCGAG 1 cut(s) 844
NmuCI GTSAC 1 cut(s) 546
NsiI ATGCAT 1 cut(s) 811
PciSI GCTCTTC 1 cut(s) 756
PcsI WCGNNNNNNNCGW 1 cut(s) 385
PctI GAATGC 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 3 cut(s) 167, 343, 445
PkrI GCNGC 2 cut(s) 75, 691
PleI GAGTC 2 cut(s) 218, 358
PpsI GAGTC 2 cut(s) 218, 358
Psp6I CCWGG 1 cut(s) 192
PspFI CCCAGC 2 cut(s) 500, 504
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 3 cut(s) 34, 150, 603
PspPI GGNCC 1 cut(s) 148
PstI CTGCAG 1 cut(s) 75
PvuII CAGCTG 1 cut(s) 504
RsaI GTAC 2 cut(s) 281, 422
RsaNI GTAC 2 cut(s) 280, 421
RseI CAYNNNNRTG 4 cut(s) 143, 467, 542, 764
SapI GCTCTTC 1 cut(s) 756
SatI GCNGC 2 cut(s) 74, 690
Sau3AI GATC 3 cut(s) 228, 642, 707
Sau96I GGNCC 1 cut(s) 148
ScaI AGTACT 1 cut(s) 422
SchI GAGTC 2 cut(s) 218, 358
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 1 cut(s) 461
SetI ASST 8 cut(s) 78, 287, 414, 506, 515, 600, 732, 751
SfaNI GCATC 2 cut(s) 642, 823
SfcI CTRYAG 1 cut(s) 71
SmiMI CAYNNNNRTG 4 cut(s) 143, 467, 542, 764
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
SpeI ACTAGT 1 cut(s) 763
Sse9I AATT 3 cut(s) 434, 528, 623
SsiI CCGC 1 cut(s) 614
SspMI CTAG 2 cut(s) 725, 764
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 3 cut(s) 217, 385, 815
TaiI ACGT 1 cut(s) 732
TaqI TCGA 2 cut(s) 227, 845
TasI AATT 3 cut(s) 434, 528, 623
TatI WGTACW 2 cut(s) 279, 420
TfiI GAWTC 3 cut(s) 167, 343, 445
TscAI CASTG 5 cut(s) 75, 388, 549, 555, 820
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 2 cut(s) 73, 689
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 2 cut(s) 339, 342
TspRI CASTG 5 cut(s) 75, 388, 549, 555, 820
XapI RAATTY 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 2 cut(s) 725, 764
ZrmI AGTACT 1 cut(s) 422
Zsp2I ATGCAT 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.