MD05G1324000.v1.1

Transmembrane protein 184C-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004499
Physical Location & Seq
Forward (+)
45092859 .. 45095627
2769 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1324000.v1.1.491

Sequence Viewer

Length: 885 bp
ATGGATTTTAGTACAATGGATCGTGCCCAGTTGACCCTGCTAGGATCCGCAGGTTGTGTGATGCTCACAATGCATTTCACAATACAATTAGTGTCGCAACATCTCTTTTACTGGAAGAACCCGAAGGAGCAGAAGGCAATAATAATTATTATTCTTATGGCTCCTATATATGCAGTTGACTCCTTTGTGGGTTTATTGGATATTAAGGGAAGCAAAACTTTTTTCATGTTTTTGGACTCTATTAAGGAATGTTACGAGGCTCTGGTGATTGCTAAGTTCTTGGCTCTTCTGTATAGTTACCTGAAGATATCCATAAGCAAAAATATCGTGCCAGATGAAATCAAAGGAAGAGAAATTCACCACTCGTTTCCTATGACTCTTTTTCAGCCTCGCACTGTTCGGCTAAACCACCAGACCCTGAAGCTACTCAAATATTGGACGTGGCAGTTTGTCATCATACGCCCAGTTTGTTCTGTTTTGATGATAACTCTACAAGCATTTGGGCTGTACCCCAGTTGGTTGAGCTGGACCTTCACTGTAATTCTTAACCTTTCAGTTTCTTTAGCGTTGTACTCTCTAGTGCTGTTTTACCATGTGTTTGCAAAGGAATTGGCACCGCATTCGCCCCTTGCAAAGTTCCTGTGCATCAAGGGAATTGTCTTCTTCGTCTTTTGGCAGGGAGTGGTCATTGACATATTAGCTGCTGTGGGCGTCATTCGATCTCACCATTTCTGGTTAGATGTGGAGCACATCGAGGAAGCTATTCAAAATGTCTTGATATGTTTGGAGATGGTTGTGTTTTCGGTTCTTCAGCAATACGCGTACCACGTTGCTCCTTACAGCGGAGACGTAGAGAAGAAGATGTTAAACAAGAAGAGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Solute_trans_a

Protein Analysis

295

Amino Acids

33.96

Weight (kDa)

8.84

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 16 - 280 1.4e-77 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 41
AccB1I GGYRCC 1 cut(s) 613
AccII CGCG 1 cut(s) 821
AciI CCGC 3 cut(s) 48, 617, 843
AclWI GGATC 3 cut(s) 27, 39, 52
AcsI RAATTY 1 cut(s) 354
AcuI CTGAAG 3 cut(s) 323, 440, 794
AcyI GRCGYC 1 cut(s) 711
AfaI GTAC 4 cut(s) 13, 509, 572, 824
AfiI CCNNNNNNNGG 1 cut(s) 842
AflIII ACRYGT 1 cut(s) 819
AgsI TTSAA 1 cut(s) 767
AjiI CACGTC 1 cut(s) 441
AluBI AGCT 4 cut(s) 424, 525, 701, 761
AluI AGCT 4 cut(s) 424, 525, 701, 761
Alw21I GWGCWC 1 cut(s) 750
Alw26I GTCTC 1 cut(s) 840
AlwI GGATC 3 cut(s) 27, 39, 52
AlwNI CAGNNNCTG 1 cut(s) 418
ApeKI GCWGC 1 cut(s) 701
ApoI RAATTY 1 cut(s) 354
Asp700I GAANNNNTTC 1 cut(s) 762
AspS9I GGNCC 1 cut(s) 528
AsuHPI GGTGA 3 cut(s) 277, 350, 716
AvaII GGWCC 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 28
BamHI GGATCC 1 cut(s) 44
BanI GGYRCC 1 cut(s) 613
BbsI GAAGAC 1 cut(s) 652
Bbv12I GWGCWC 1 cut(s) 750
BbvI GCAGC 1 cut(s) 688
BccI CCATC 1 cut(s) 784
BcgI CGANNNNNNTGC 4 cut(s) 307, 341, 603, 637
BcoDI GTCTC 1 cut(s) 840
BfaI CTAG 2 cut(s) 41, 578
BfuAI ACCTGC 1 cut(s) 41
BisI GCNGC 1 cut(s) 702
BlsI GCNGC 1 cut(s) 703
Bme18I GGWCC 1 cut(s) 528
BmgBI CACGTC 1 cut(s) 441
BmgT120I GGNCC 1 cut(s) 528
BmiI GGNNCC 3 cut(s) 46, 162, 615
BmrI ACTGGG 3 cut(s) 22, 458, 507
BmsI GCATC 2 cut(s) 51, 654
BmuI ACTGGG 3 cut(s) 22, 458, 507
BpiI GAAGAC 1 cut(s) 652
BsaHI GRCGYC 1 cut(s) 711
Bsc4I CCNNNNNNNGG 1 cut(s) 842
Bse1I ACTGG 4 cut(s) 28, 116, 464, 513
BseLI CCNNNNNNNGG 1 cut(s) 842
BseNI ACTGG 4 cut(s) 28, 116, 464, 513
BseSI GKGCMC 1 cut(s) 28
BseXI GCAGC 1 cut(s) 688
Bsh1236I CGCG 1 cut(s) 821
BshNI GGYRCC 1 cut(s) 613
BsiHKAI GWGCWC 1 cut(s) 750
BslI CCNNNNNNNGG 1 cut(s) 842
BsmAI GTCTC 1 cut(s) 840
BsmBI CGTCTC 1 cut(s) 840
BsmI GAATGC 1 cut(s) 619
Bsp1286I GDGCHC 2 cut(s) 28, 750
Bsp143I GATC 3 cut(s) 19, 44, 719
BspACI CCGC 3 cut(s) 48, 617, 843
BspFNI CGCG 1 cut(s) 821
BspLI GGNNCC 3 cut(s) 46, 162, 615
BspMI ACCTGC 1 cut(s) 41
BspPI GGATC 3 cut(s) 27, 39, 52
BspQI GCTCTTC 1 cut(s) 291
BspT107I GGYRCC 1 cut(s) 613
BsrI ACTGG 4 cut(s) 28, 116, 464, 513
BssMI GATC 3 cut(s) 19, 44, 719
BssNI GRCGYC 1 cut(s) 711
Bst4CI ACNGT 2 cut(s) 397, 538
Bst6I CTCTTC 3 cut(s) 291, 343, 869
BstACI GRCGYC 1 cut(s) 711
BstDEI CTNAG 1 cut(s) 273
BstFNI CGCG 1 cut(s) 821
BstKTI GATC 3 cut(s) 22, 47, 722
BstMAI GTCTC 1 cut(s) 840
BstMBI GATC 3 cut(s) 19, 44, 719
BstMWI GCNNNNNNNGC 1 cut(s) 70
BstSLI GKGCMC 1 cut(s) 28
BstUI CGCG 1 cut(s) 821
BstV1I GCAGC 1 cut(s) 688
BstV2I GAAGAC 1 cut(s) 652
BstX2I RGATCY 1 cut(s) 44
BstYI RGATCY 1 cut(s) 44
BtrI CACGTC 1 cut(s) 441
BtsIMutI CAGTG 2 cut(s) 393, 534
BveI ACCTGC 1 cut(s) 41
CaiI CAGNNNCTG 1 cut(s) 418
Cfr13I GGNCC 1 cut(s) 528
CseI GACGC 1 cut(s) 700
Csp6I GTAC 4 cut(s) 12, 508, 571, 823
CviAII CATG 2 cut(s) 226, 593
CviQI GTAC 4 cut(s) 12, 508, 571, 823
DdeI CTNAG 1 cut(s) 273
DpnI GATC 3 cut(s) 21, 46, 721
DpnII GATC 3 cut(s) 19, 44, 719
Eam1104I CTCTTC 3 cut(s) 291, 343, 869
EarI CTCTTC 3 cut(s) 291, 343, 869
Eco32I GATATC 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 528
Eco57I CTGAAG 3 cut(s) 323, 440, 794
EcoRV GATATC 1 cut(s) 309
EcoT22I ATGCAT 1 cut(s) 75
Esp3I CGTCTC 1 cut(s) 840
FaeI CATG 2 cut(s) 229, 596
FalI AAGNNNNNCTT 2 cut(s) 202, 234
FatI CATG 2 cut(s) 225, 592
Fnu4HI GCNGC 1 cut(s) 702
Fsp4HI GCNGC 1 cut(s) 702
FspBI CTAG 2 cut(s) 41, 578
GluI GCNGC 1 cut(s) 702
HgaI GACGC 1 cut(s) 700
Hin1I GRCGYC 1 cut(s) 711
Hin1II CATG 2 cut(s) 229, 596
HincII GTYRAC 2 cut(s) 33, 178
HindII GTYRAC 2 cut(s) 33, 178
HinfI GANTC 3 cut(s) 179, 236, 376
HphI GGTGA 3 cut(s) 277, 350, 716
Hpy166II GTNNAC 2 cut(s) 33, 178
Hpy188III TCNNGA 1 cut(s) 775
Hpy8I GTNNAC 2 cut(s) 33, 178
HpyAV CCTTC 3 cut(s) 118, 127, 541
HpyCH4III ACNGT 2 cut(s) 397, 538
HpyCH4IV ACGT 3 cut(s) 440, 828, 849
HpyCH4V TGCA 5 cut(s) 73, 173, 602, 632, 645
HpyF10VI GCNNNNNNNGC 1 cut(s) 70
HpyF3I CTNAG 1 cut(s) 273
HpySE526I ACGT 3 cut(s) 440, 828, 849
Hsp92I GRCGYC 1 cut(s) 711
Hsp92II CATG 2 cut(s) 229, 596
Kzo9I GATC 3 cut(s) 19, 44, 719
LguI GCTCTTC 1 cut(s) 291
LmnI GCTCC 4 cut(s) 127, 166, 745, 838
Lsp1109I GCAGC 1 cut(s) 688
LweI GCATC 2 cut(s) 51, 654
MaeI CTAG 2 cut(s) 41, 578
MaeII ACGT 3 cut(s) 440, 828, 849
MaeIII GTNAC 2 cut(s) 251, 296
MalI GATC 3 cut(s) 21, 46, 721
MboI GATC 3 cut(s) 19, 44, 719
MboII GAAGA 9 cut(s) 127, 278, 316, 360, 652, 655, 800, 868, 871
MflI RGATCY 1 cut(s) 44
MhlI GDGCHC 2 cut(s) 28, 750
MluCI AATT 6 cut(s) 86, 144, 354, 540, 608, 654
MluI ACGCGT 1 cut(s) 819
MlyI GAGTC 3 cut(s) 173, 230, 370
MnlI CCTC 4 cut(s) 250, 399, 748, 870
Mph1103I ATGCAT 1 cut(s) 75
MroXI GAANNNNTTC 1 cut(s) 762
MseI TTAA 4 cut(s) 204, 243, 546, 866
MspA1I CMGCKG 1 cut(s) 843
Mva1269I GAATGC 1 cut(s) 619
MvnI CGCG 1 cut(s) 821
MwoI GCNNNNNNNGC 1 cut(s) 70
NdeII GATC 3 cut(s) 19, 44, 719
NlaIII CATG 2 cut(s) 229, 596
NlaIV GGNNCC 3 cut(s) 46, 162, 615
NsiI ATGCAT 1 cut(s) 75
PciSI GCTCTTC 1 cut(s) 291
PcsI WCGNNNNNNNCGW 1 cut(s) 825
PctI GAATGC 1 cut(s) 619
PdmI GAANNNNTTC 1 cut(s) 762
PkrI GCNGC 1 cut(s) 703
PleI GAGTC 3 cut(s) 173, 230, 370
PpsI GAGTC 3 cut(s) 173, 230, 370
PspN4I GGNNCC 3 cut(s) 46, 162, 615
PspPI GGNCC 1 cut(s) 528
PstNI CAGNNNCTG 1 cut(s) 418
PsuI RGATCY 1 cut(s) 44
RsaI GTAC 4 cut(s) 13, 509, 572, 824
RsaNI GTAC 4 cut(s) 12, 508, 571, 823
SapI GCTCTTC 1 cut(s) 291
SaqAI TTAA 4 cut(s) 204, 243, 546, 866
SatI GCNGC 1 cut(s) 702
Sau3AI GATC 3 cut(s) 19, 44, 719
Sau96I GGNCC 1 cut(s) 528
SchI GAGTC 3 cut(s) 173, 230, 370
SduI GDGCHC 2 cut(s) 28, 750
SfaNI GCATC 2 cut(s) 51, 654
SinI GGWCC 1 cut(s) 528
Sse9I AATT 6 cut(s) 86, 144, 354, 540, 608, 654
SsiI CCGC 3 cut(s) 48, 617, 843
SspI AATATT 1 cut(s) 434
SspMI CTAG 2 cut(s) 41, 578
TaaI ACNGT 2 cut(s) 397, 538
TaiI ACGT 3 cut(s) 443, 831, 852
TaqI TCGA 2 cut(s) 718, 753
TasI AATT 6 cut(s) 86, 144, 354, 540, 608, 654
TatI WGTACW 2 cut(s) 11, 570
Tru1I TTAA 4 cut(s) 204, 243, 546, 866
Tru9I TTAA 4 cut(s) 204, 243, 546, 866
TscAI CASTG 2 cut(s) 400, 541
TseI GCWGC 1 cut(s) 701
TspDTI ATGAA 2 cut(s) 214, 351
TspRI CASTG 2 cut(s) 400, 541
VpaK11BI GGWCC 1 cut(s) 528
XapI RAATTY 1 cut(s) 354
XmnI GAANNNNTTC 1 cut(s) 762
XspI CTAG 2 cut(s) 41, 578
Zsp2I ATGCAT 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.