Rh5DG251700

Organic solute transporter Ostalpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004499
Physical Location & Seq
Forward (+)
29062644 .. 29065676
3033 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG251700.1

Sequence Viewer

Length: 876 bp
ATGGCAAACCCTGCACAGATTACTCTTATGGGCTCCACTTTCTGTGTGATGCTTACACTGCATTTCTCAGTACAGCTTCTCTCAGAGCACTTGTTCTGCTGGAAGAAGCCAAAGGAGCAAAAGGCCATACTCATCATTATACTTATGGCCCCCTTGTATGCCATAGATTCCTATGTGGGCTTGATTGATTTCCAGGGAAGCAAAGCTTTCTTTATGTTCTTGGACTCGGTCAAGGAGTGCTATGAAGCTTTGGTGATAGCCAAGTTCTTGGCTTTGCTGTATAGCTACTTGAACATATCGATAAGTAAAAACATAGTGCCTGATGAGATCAAAGGAAGGGAGATTCACCATTCGTTTCCAATGACTCTGTTTGTGCCTCGCTCTGTTCGTTTGAACCATCATACACTGAAGCTTCTCAAATACTGGACATGGCAATTTGTTGTTATTCGCCCTGTGTGCTCCATCTTGATGATTAGTCTTCAACTTATTGGAGTATATCCCAGTTGGGTTAGCTGGACATTCACTATAATTTTGAACATTTCAGTGTCACTGGCTCTGTATTCTCTTGTCATTTTCTACCATGTCTTTGATAAGGAGCTGGCACCACACAAACCTCTTGCTAAGTTCTTGTGCATCAAAGGGATTGTCTTCTTCTGCTTTTGGCAGGGAATTGTTCTTGATATTCTCGTAGCATTAAAAATAATTCGATCTCATCATAAATGGCTTGACGTGGAGCATATTGAGGAAGCGCTGCAGAACATGTTAGTGTGTGTGGAGATGGTTTTCTTCTCGATTGTTCAGAAGTATGCCTACGGTGCTGAACCTTATAGAGATGATGCTATATCAACTGCAAAACGGGATAAGAAGACGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Solute_trans_a

Protein Analysis

291

Amino Acids

33.69

Weight (kDa)

8.72

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 10 - 276 3e-76 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 601
AcuI CTGAAG 1 cut(s) 428
AfaI GTAC 1 cut(s) 72
AfeI AGCGCT 1 cut(s) 750
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 4 cut(s) 292, 394, 482, 535
AjiI CACGTC 1 cut(s) 730
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 7 cut(s) 76, 206, 248, 285, 412, 513, 598
AluI AGCT 7 cut(s) 76, 206, 248, 285, 412, 513, 598
Alw21I GWGCWC 2 cut(s) 90, 461
Aor51HI AGCGCT 1 cut(s) 750
AoxI GGCC 2 cut(s) 123, 147
ApeKI GCWGC 1 cut(s) 751
ArsI GACNNNNNNTTYG 2 cut(s) 630, 662
AspLEI GCGC 1 cut(s) 751
AspS9I GGNCC 1 cut(s) 148
AsuHPI GGTGA 2 cut(s) 265, 338
BanI GGYRCC 1 cut(s) 601
BanII GRGCYC 1 cut(s) 35
BarI GAAGNNNNNNTAC 2 cut(s) 794, 826
BbsI GAAGAC 3 cut(s) 470, 640, 872
Bbv12I GWGCWC 2 cut(s) 90, 461
BbvI GCAGC 1 cut(s) 738
BccI CCATC 3 cut(s) 405, 470, 772
BciT130I CCWGG 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 752
BfoI RGCGCY 1 cut(s) 752
BisI GCNGC 1 cut(s) 752
BlsI GCNGC 1 cut(s) 753
Bme1390I CCNGG 1 cut(s) 194
BmgBI CACGTC 1 cut(s) 730
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 3 cut(s) 34, 150, 603
BmrFI CCNGG 1 cut(s) 194
BmrI ACTGGG 1 cut(s) 495
BmsI GCATC 3 cut(s) 39, 642, 826
BmuI ACTGGG 1 cut(s) 495
BpiI GAAGAC 3 cut(s) 470, 640, 872
Bsa29I ATCGAT 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 193
Bse1I ACTGG 3 cut(s) 428, 501, 555
BseBI CCWGG 1 cut(s) 194
BseCI ATCGAT 1 cut(s) 299
BseDI CCNNGG 1 cut(s) 193
BseMII CTCAG 2 cut(s) 81, 96
BseNI ACTGG 3 cut(s) 428, 501, 555
BseXI GCAGC 1 cut(s) 738
BshFI GGCC 2 cut(s) 125, 149
BshNI GGYRCC 1 cut(s) 601
BshVI ATCGAT 1 cut(s) 299
BsiHKAI GWGCWC 2 cut(s) 90, 461
BsnI GGCC 2 cut(s) 125, 149
Bsp1286I GDGCHC 3 cut(s) 35, 90, 461
Bsp143I GATC 2 cut(s) 327, 707
BspANI GGCC 2 cut(s) 125, 149
BspCNI CTCAG 2 cut(s) 80, 95
BspDI ATCGAT 1 cut(s) 299
BspLI GGNNCC 3 cut(s) 34, 150, 603
BspMAI CTGCAG 1 cut(s) 756
BspT107I GGYRCC 1 cut(s) 601
BsrI ACTGG 3 cut(s) 428, 501, 555
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 2 cut(s) 327, 707
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 1 cut(s) 815
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 3 cut(s) 67, 82, 621
BstH2I RGCGCY 1 cut(s) 752
BstHHI GCGC 1 cut(s) 751
BstKTI GATC 2 cut(s) 330, 710
BstMBI GATC 2 cut(s) 327, 707
BstMWI GCNNNNNNNGC 5 cut(s) 11, 58, 115, 456, 815
BstNI CCWGG 1 cut(s) 194
BstNSI RCATGY 1 cut(s) 763
BstSCI CCNGG 1 cut(s) 192
BstSFI CTRYAG 1 cut(s) 752
BstV1I GCAGC 1 cut(s) 738
BstV2I GAAGAC 3 cut(s) 470, 640, 872
BstXI CCANNNNNNTGG 1 cut(s) 268
Bsu15I ATCGAT 1 cut(s) 299
BsuRI GGCC 2 cut(s) 125, 149
BsuTUI ATCGAT 1 cut(s) 299
BtrI CACGTC 1 cut(s) 730
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 4 cut(s) 56, 404, 548, 549
Cac8I GCNNGC 1 cut(s) 600
CfoI GCGC 1 cut(s) 751
Cfr13I GGNCC 1 cut(s) 148
ClaI ATCGAT 1 cut(s) 299
Csp6I GTAC 1 cut(s) 71
CviAII CATG 3 cut(s) 429, 581, 760
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 3 cut(s) 67, 82, 621
DpnI GATC 2 cut(s) 329, 709
DpnII GATC 2 cut(s) 327, 707
Eco24I GRGCYC 1 cut(s) 35
Eco47III AGCGCT 1 cut(s) 750
Eco57I CTGAAG 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 192
EcoT38I GRGCYC 1 cut(s) 35
FaeI CATG 3 cut(s) 432, 584, 763
FalI AAGNNNNNCTT 2 cut(s) 190, 222
FatI CATG 3 cut(s) 428, 580, 759
Fnu4HI GCNGC 1 cut(s) 752
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 1 cut(s) 752
GlaI GCGC 1 cut(s) 750
GluI GCNGC 1 cut(s) 752
HaeII RGCGCY 1 cut(s) 752
HaeIII GGCC 2 cut(s) 125, 149
HhaI GCGC 1 cut(s) 751
Hin1II CATG 3 cut(s) 432, 584, 763
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HindIII AAGCTT 3 cut(s) 204, 246, 410
HinfI GANTC 4 cut(s) 167, 224, 343, 364
HphI GGTGA 2 cut(s) 265, 338
Hpy188I TCNGA 2 cut(s) 85, 801
Hpy188III TCNNGA 3 cut(s) 466, 677, 790
HpyAV CCTTC 1 cut(s) 330
HpyCH4III ACNGT 1 cut(s) 815
HpyCH4IV ACGT 1 cut(s) 729
HpyCH4V TGCA 5 cut(s) 14, 61, 633, 754, 851
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 58, 115, 456, 815
HpyF3I CTNAG 3 cut(s) 67, 82, 621
HpySE526I ACGT 1 cut(s) 729
Hsp92II CATG 3 cut(s) 432, 584, 763
HspAI GCGC 1 cut(s) 749
Kzo9I GATC 2 cut(s) 327, 707
LmnI GCTCC 5 cut(s) 38, 115, 464, 595, 733
Lsp1109I GCAGC 1 cut(s) 738
LweI GCATC 3 cut(s) 39, 642, 826
MaeII ACGT 1 cut(s) 729
MaeIII GTNAC 1 cut(s) 546
MalI GATC 2 cut(s) 329, 709
MboI GATC 2 cut(s) 327, 707
MboII GAAGA 5 cut(s) 115, 470, 640, 643, 778
MhlI GDGCHC 3 cut(s) 35, 90, 461
MluCI AATT 4 cut(s) 434, 528, 669, 702
MlyI GAGTC 2 cut(s) 218, 358
MnlI CCTC 3 cut(s) 387, 624, 736
MseI TTAA 1 cut(s) 695
MslI CAYNNNNRTG 3 cut(s) 467, 542, 764
MspR9I CCNGG 1 cut(s) 194
MvaI CCWGG 1 cut(s) 194
MwoI GCNNNNNNNGC 5 cut(s) 11, 58, 115, 456, 815
NdeII GATC 2 cut(s) 327, 707
NlaIII CATG 3 cut(s) 432, 584, 763
NlaIV GGNNCC 3 cut(s) 34, 150, 603
NmuCI GTSAC 1 cut(s) 546
NspI RCATGY 1 cut(s) 763
PciI ACATGT 1 cut(s) 759
PcsI WCGNNNNNNNCGW 1 cut(s) 385
PfeI GAWTC 2 cut(s) 167, 343
PflFI GACNNNGTC 1 cut(s) 227
PkrI GCNGC 1 cut(s) 753
PleI GAGTC 2 cut(s) 218, 358
PpsI GAGTC 2 cut(s) 218, 358
PscI ACATGT 1 cut(s) 759
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 3 cut(s) 34, 150, 603
PspPI GGNCC 1 cut(s) 148
PstI CTGCAG 1 cut(s) 756
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
RseI CAYNNNNRTG 3 cut(s) 467, 542, 764
SaqAI TTAA 1 cut(s) 695
SatI GCNGC 1 cut(s) 752
Sau3AI GATC 2 cut(s) 327, 707
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 2 cut(s) 218, 358
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 3 cut(s) 35, 90, 461
SfaNI GCATC 3 cut(s) 39, 642, 826
SfcI CTRYAG 1 cut(s) 752
SmiMI CAYNNNNRTG 3 cut(s) 467, 542, 764
Sse9I AATT 4 cut(s) 434, 528, 669, 702
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 815
TaiI ACGT 1 cut(s) 732
TaqI TCGA 3 cut(s) 299, 706, 791
TaqII GACCGA 1 cut(s) 217
TasI AATT 4 cut(s) 434, 528, 669, 702
TatI WGTACW 1 cut(s) 70
TfiI GAWTC 2 cut(s) 167, 343
Tru1I TTAA 1 cut(s) 695
Tru9I TTAA 1 cut(s) 695
TscAI CASTG 4 cut(s) 63, 411, 549, 555
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 1 cut(s) 751
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 1 cut(s) 258
TspRI CASTG 4 cut(s) 63, 411, 549, 555
Tth111I GACNNNGTC 1 cut(s) 227
XceI RCATGY 1 cut(s) 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.