MD03G1225100.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008883
Physical Location & Seq
Forward (+)
31080936 .. 31081421
486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1225100.v1.1.491

Sequence Viewer

Length: 486 bp
ATGATGAGAACATGTCCAGAATCTGCAGCATTTCCCAGCCCTACCCTTCTTGGAAACGAATCTGATCGCTTGGCTTTGCTAGACTTCAAGAAAAGAATAACTGAAGATCCCCTCAGTGTCATGAGCTCGTGGAATCATTCCATCCACTTCTGCAGTTGGGTAGGCATTACATGCCACCGTGCCACCCAAAGAGTCTTGATTCCGGACCTGGAAGATAAACAATTGGTAGGTTCCATTCCACCTTCCATTGGAAATCTTACTTGTCTAATTGAAATAAGCTTGGGAATCAACAAGTTTCATGGGGAAATTCCTCAAGAACTGGGTCGACTACGTACCCTGAAAAGTCTAAACCTGTCTTTCAATTCTTTAGGCGGGAAAATTCCGATTAATATGTCCCACTGTACACAACTAAGATTTTTCGATCTTTTTTCCAATCCCCATAAGATTATGGACGTTATGAAAATTGCCTTCACTATAAAAGTATAA

Protein Analysis

162

Amino Acids

18.08

Weight (kDa)

8.68

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 20 - 58 8.4e-13 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 325
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 1 cut(s) 372
AclWI GGATC 1 cut(s) 101
AcsI RAATTY 2 cut(s) 306, 378
AcuI CTGAAG 1 cut(s) 123
AfaI GTAC 2 cut(s) 334, 403
AfiI CCNNNNNNNGG 1 cut(s) 248
AflIII ACRYGT 1 cut(s) 11
AgsI TTSAA 3 cut(s) 88, 272, 361
AjnI CCWGG 1 cut(s) 207
AluBI AGCT 2 cut(s) 126, 279
AluI AGCT 2 cut(s) 126, 279
Alw21I GWGCWC 1 cut(s) 128
AlwI GGATC 1 cut(s) 101
AlwNI CAGNNNCTG 1 cut(s) 23
Aor13HI TCCGGA 1 cut(s) 202
ApeKI GCWGC 1 cut(s) 26
ApoI RAATTY 2 cut(s) 306, 378
AseI ATTAAT 1 cut(s) 387
AspS9I GGNCC 1 cut(s) 205
AvaII GGWCC 1 cut(s) 205
BanII GRGCYC 1 cut(s) 128
BauI CACGAG 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 128
BbvI GCAGC 1 cut(s) 38
BccI CCATC 1 cut(s) 149
BciT130I CCWGG 1 cut(s) 209
BfaI CTAG 1 cut(s) 80
BfmI CTRYAG 2 cut(s) 24, 151
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
Bme1390I CCNGG 1 cut(s) 209
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 1 cut(s) 232
BmrFI CCNGG 1 cut(s) 209
BmrI ACTGGG 1 cut(s) 329
BmuI ACTGGG 1 cut(s) 329
BpuEI CTTGAG 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 332
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 324
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 1 cut(s) 209
BseGI GGATG 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 248
BseMII CTCAG 1 cut(s) 127
BseNI ACTGG 1 cut(s) 324
BseXI GCAGC 1 cut(s) 38
BseYI CCCAGC 1 cut(s) 35
BsiHKAI GWGCWC 1 cut(s) 128
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 1 cut(s) 248
BsmFI GGGAC 1 cut(s) 379
Bsp1286I GDGCHC 1 cut(s) 128
Bsp13I TCCGGA 1 cut(s) 202
Bsp1407I TGTACA 1 cut(s) 401
Bsp143I GATC 3 cut(s) 64, 106, 421
BspACI CCGC 1 cut(s) 372
BspCNI CTCAG 1 cut(s) 126
BspEI TCCGGA 1 cut(s) 202
BspHI TCATGA 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 232
BspMAI CTGCAG 2 cut(s) 28, 155
BspPI GGATC 1 cut(s) 101
BsrGI TGTACA 1 cut(s) 401
BsrI ACTGG 1 cut(s) 324
BssMI GATC 3 cut(s) 64, 106, 421
BssSI CACGAG 1 cut(s) 127
Bst2BI CACGAG 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 2 cut(s) 179, 401
BstAPI GCANNNNNTGC 1 cut(s) 171
BstAUI TGTACA 1 cut(s) 401
BstBAI YACGTR 1 cut(s) 332
BstDEI CTNAG 2 cut(s) 113, 410
BstF5I GGATG 1 cut(s) 141
BstKTI GATC 3 cut(s) 67, 109, 424
BstMBI GATC 3 cut(s) 64, 106, 421
BstMWI GCNNNNNNNGC 1 cut(s) 171
BstNI CCWGG 1 cut(s) 209
BstNSI RCATGY 2 cut(s) 15, 174
BstSCI CCNGG 1 cut(s) 207
BstSFI CTRYAG 2 cut(s) 24, 151
BstSNI TACGTA 1 cut(s) 332
BstV1I GCAGC 1 cut(s) 38
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BtsCI GGATG 1 cut(s) 141
BtsIMutI CAGTG 2 cut(s) 121, 397
CaiI CAGNNNCTG 1 cut(s) 23
CciI TCATGA 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 205
Csp6I GTAC 2 cut(s) 333, 402
CviAII CATG 4 cut(s) 12, 121, 171, 299
CviJI RGCY 4 cut(s) 39, 74, 126, 279
CviKI_1 RGCY 4 cut(s) 39, 74, 126, 279
CviQI GTAC 2 cut(s) 333, 402
DdeI CTNAG 2 cut(s) 113, 410
DpnI GATC 3 cut(s) 66, 108, 423
DpnII GATC 3 cut(s) 64, 106, 421
Ecl136II GAGCTC 1 cut(s) 126
Eco105I TACGTA 1 cut(s) 332
Eco24I GRGCYC 1 cut(s) 128
Eco47I GGWCC 1 cut(s) 205
Eco53kI GAGCTC 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 123
EcoICRI GAGCTC 1 cut(s) 126
EcoRII CCWGG 1 cut(s) 207
EcoT38I GRGCYC 1 cut(s) 128
FaeI CATG 4 cut(s) 15, 124, 174, 302
FaqI GGGAC 1 cut(s) 379
FatI CATG 4 cut(s) 11, 120, 170, 298
FauI CCCGC 1 cut(s) 365
FblI GTMKAC 1 cut(s) 325
Fnu4HI GCNGC 1 cut(s) 27
FokI GGATG 1 cut(s) 128
FriOI GRGCYC 1 cut(s) 128
Fsp4HI GCNGC 1 cut(s) 27
FspBI CTAG 1 cut(s) 80
GluI GCNGC 1 cut(s) 27
GsaI CCCAGC 1 cut(s) 39
HapII CCGG 1 cut(s) 203
Hin1II CATG 4 cut(s) 15, 124, 174, 302
HincII GTYRAC 1 cut(s) 326
HindII GTYRAC 1 cut(s) 326
HindIII AAGCTT 1 cut(s) 277
HinfI GANTC 6 cut(s) 20, 59, 133, 192, 199, 285
HpaII CCGG 1 cut(s) 203
Hpy166II GTNNAC 2 cut(s) 326, 404
Hpy188I TCNGA 2 cut(s) 64, 384
Hpy188III TCNNGA 6 cut(s) 17, 88, 121, 196, 203, 314
Hpy8I GTNNAC 2 cut(s) 326, 404
HpyAV CCTTC 3 cut(s) 56, 252, 478
HpyCH4III ACNGT 2 cut(s) 179, 401
HpyCH4IV ACGT 2 cut(s) 331, 453
HpyCH4V TGCA 2 cut(s) 26, 153
HpyF10VI GCNNNNNNNGC 1 cut(s) 171
HpyF3I CTNAG 2 cut(s) 113, 410
HpySE526I ACGT 2 cut(s) 331, 453
Hsp92II CATG 4 cut(s) 15, 124, 174, 302
Kpn2I TCCGGA 1 cut(s) 202
Kzo9I GATC 3 cut(s) 64, 106, 421
LpnPI CCDG 8 cut(s) 30, 49, 194, 216, 221, 305, 350, 365
Lsp1109I GCAGC 1 cut(s) 38
MaeI CTAG 1 cut(s) 80
MaeII ACGT 2 cut(s) 331, 453
MalI GATC 3 cut(s) 66, 108, 423
MboI GATC 3 cut(s) 64, 106, 421
MboII GAAGA 2 cut(s) 116, 224
MfeI CAATTG 1 cut(s) 221
MflI RGATCY 1 cut(s) 106
MhlI GDGCHC 1 cut(s) 128
MluCI AATT 6 cut(s) 221, 267, 306, 361, 378, 462
MlyI GAGTC 1 cut(s) 201
MnlI CCTC 2 cut(s) 122, 321
MroI TCCGGA 1 cut(s) 202
MseI TTAA 1 cut(s) 387
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 209
MunI CAATTG 1 cut(s) 221
MvaI CCWGG 1 cut(s) 209
MwoI GCNNNNNNNGC 1 cut(s) 171
NdeII GATC 3 cut(s) 64, 106, 421
NlaIII CATG 4 cut(s) 15, 124, 174, 302
NlaIV GGNNCC 1 cut(s) 232
NspI RCATGY 2 cut(s) 15, 174
PagI TCATGA 1 cut(s) 120
PciI ACATGT 1 cut(s) 11
PfeI GAWTC 5 cut(s) 20, 59, 133, 199, 285
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
Ppu21I YACGTR 1 cut(s) 332
PscI ACATGT 1 cut(s) 11
PshBI ATTAAT 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 207
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 1 cut(s) 232
PspPI GGNCC 1 cut(s) 205
PstI CTGCAG 2 cut(s) 28, 155
PstNI CAGNNNCTG 1 cut(s) 23
PsuI RGATCY 1 cut(s) 106
RsaI GTAC 2 cut(s) 334, 403
RsaNI GTAC 2 cut(s) 333, 402
SacI GAGCTC 1 cut(s) 128
SalI GTCGAC 1 cut(s) 324
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 3 cut(s) 64, 106, 421
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 1 cut(s) 201
ScrFI CCNGG 1 cut(s) 209
SduI GDGCHC 1 cut(s) 128
SetI ASST 8 cut(s) 128, 210, 232, 244, 281, 334, 354, 456
SfcI CTRYAG 2 cut(s) 24, 151
SinI GGWCC 1 cut(s) 205
SmlI CTYRAG 1 cut(s) 312
SmoI CTYRAG 1 cut(s) 312
SnaBI TACGTA 1 cut(s) 332
Sse9I AATT 6 cut(s) 221, 267, 306, 361, 378, 462
SsiI CCGC 1 cut(s) 372
SspMI CTAG 1 cut(s) 80
SstI GAGCTC 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 207
TaaI ACNGT 2 cut(s) 179, 401
TaiI ACGT 2 cut(s) 334, 456
TaqI TCGA 2 cut(s) 325, 420
TasI AATT 6 cut(s) 221, 267, 306, 361, 378, 462
TatI WGTACW 1 cut(s) 401
TfiI GAWTC 5 cut(s) 20, 59, 133, 199, 285
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 2 cut(s) 121, 404
TseI GCWGC 1 cut(s) 26
TspDTI ATGAA 2 cut(s) 287, 473
TspRI CASTG 2 cut(s) 121, 404
VpaK11BI GGWCC 1 cut(s) 205
VspI ATTAAT 1 cut(s) 387
XapI RAATTY 2 cut(s) 306, 378
XceI RCATGY 2 cut(s) 15, 174
XmiI GTMKAC 1 cut(s) 325
XspI CTAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.