MD04G1149900.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008883
Physical Location & Seq
Forward (+)
23871283 .. 23871657
375 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1149900.v1.1.491

Sequence Viewer

Length: 375 bp
ATGGAGCTTTCGTATACTTGCTGCATGAAACTTCTGTTTAAATTCCTTCCCGGGTTCTTTCTCTTATGCATGATCACGTGCCCGGAATTCGTCTTTGGAAACGAATCTGACCGCCTCGCTCTGCTGGACTTGAAGAGAAGAATAACAAATGATCCTCTCCATATCATGAACTCGTGGAATGATTCGACAGATTTGTGCAGTTGGATTGGCGTTACATGCAACGTTTCAAACAAAAGAGTCGTGATCTTGAACTTGGAAGCTCAAAAACTGGTAGGCTCCATACCACCTTCTATCGGAAACCTTACTTATCTTACTGGAATCAACTTCATAGACAACAATTTTTATGGTGAAATTCCTCAAGAATGGGACGTCTAG

Protein Analysis

125

Amino Acids

14.16

Weight (kDa)

4.78

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 35 - 74 1e-12 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 372
AccI GTMKAC 1 cut(s) 14
AciI CCGC 1 cut(s) 112
AclI AACGTT 1 cut(s) 222
AclWI GGATC 1 cut(s) 146
AcsI RAATTY 3 cut(s) 41, 86, 351
AcvI CACGTG 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 369
AfiI CCNNNNNNNGG 1 cut(s) 293
AgsI TTSAA 3 cut(s) 133, 228, 250
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 2 cut(s) 7, 260
AluI AGCT 2 cut(s) 7, 260
AlwI GGATC 1 cut(s) 146
Ama87I CYCGRG 1 cut(s) 50
ApeKI GCWGC 1 cut(s) 21
ApoI RAATTY 3 cut(s) 41, 86, 351
AsuC2I CCSGG 3 cut(s) 51, 52, 83
AsuHPI GGTGA 1 cut(s) 359
AvaI CYCGRG 1 cut(s) 50
BaeGI GKGCMC 1 cut(s) 83
BauI CACGAG 1 cut(s) 172
BbrPI CACGTG 1 cut(s) 78
BbvI GCAGC 1 cut(s) 8
BclI TGATCA 1 cut(s) 72
BcnI CCSGG 3 cut(s) 51, 52, 83
BfaI CTAG 1 cut(s) 373
BisI GCNGC 1 cut(s) 22
BlsI GCNGC 1 cut(s) 23
Bme1390I CCNGG 3 cut(s) 51, 52, 83
BmeT110I CYCGRG 1 cut(s) 50
BmiI GGNNCC 1 cut(s) 277
BmrFI CCNGG 3 cut(s) 51, 52, 83
BpuEI CTTGAG 1 cut(s) 342
BpuMI CCSGG 3 cut(s) 51, 52, 83
BsaAI YACGTR 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 50
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 2 cut(s) 273, 319
BseDI CCNNGG 1 cut(s) 50
BseLI CCNNNNNNNGG 1 cut(s) 293
BseNI ACTGG 2 cut(s) 273, 319
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 1 cut(s) 8
BsgI GTGCAG 1 cut(s) 217
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 2 cut(s) 51, 83
BslI CCNNNNNNNGG 1 cut(s) 293
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 83
Bsp143I GATC 3 cut(s) 72, 151, 243
BspACI CCGC 1 cut(s) 112
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 277
BspPI GGATC 1 cut(s) 146
BsrI ACTGG 2 cut(s) 273, 319
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 3 cut(s) 72, 151, 243
BssNAI GTATAC 1 cut(s) 15
BssNI GRCGYC 1 cut(s) 369
BssSI CACGAG 1 cut(s) 172
Bst1107I GTATAC 1 cut(s) 15
Bst2BI CACGAG 1 cut(s) 172
Bst6I CTCTTC 1 cut(s) 128
BstACI GRCGYC 1 cut(s) 369
BstBAI YACGTR 1 cut(s) 78
BstKTI GATC 3 cut(s) 75, 154, 246
BstMBI GATC 3 cut(s) 72, 151, 243
BstMWI GCNNNNNNNGC 1 cut(s) 216
BstNSI RCATGY 1 cut(s) 219
BstSCI CCNGG 3 cut(s) 49, 50, 81
BstSLI GKGCMC 1 cut(s) 83
BstV1I GCAGC 1 cut(s) 8
BstZ17I GTATAC 1 cut(s) 15
CciI TCATGA 1 cut(s) 165
Cfr9I CCCGGG 1 cut(s) 50
CviAII CATG 4 cut(s) 25, 70, 166, 216
CviJI RGCY 3 cut(s) 7, 260, 276
CviKI_1 RGCY 3 cut(s) 7, 260, 276
DpnI GATC 3 cut(s) 74, 153, 245
DpnII GATC 3 cut(s) 72, 151, 243
DraI TTTAAA 1 cut(s) 40
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Eco72I CACGTG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 50
EcoRI GAATTC 1 cut(s) 86
EcoT22I ATGCAT 1 cut(s) 71
FaeI CATG 4 cut(s) 28, 73, 169, 219
FatI CATG 4 cut(s) 24, 69, 165, 215
FbaI TGATCA 1 cut(s) 72
FblI GTMKAC 1 cut(s) 14
Fnu4HI GCNGC 1 cut(s) 22
Fsp4HI GCNGC 1 cut(s) 22
FspBI CTAG 1 cut(s) 373
GluI GCNGC 1 cut(s) 22
HapII CCGG 2 cut(s) 51, 83
Hin1I GRCGYC 1 cut(s) 369
Hin1II CATG 4 cut(s) 28, 73, 169, 219
HinfI GANTC 4 cut(s) 104, 182, 237, 318
HpaII CCGG 2 cut(s) 51, 83
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 2 cut(s) 109, 296
Hpy188III TCNNGA 4 cut(s) 166, 241, 247, 359
Hpy8I GTNNAC 1 cut(s) 15
HpyAV CCTTC 2 cut(s) 56, 297
HpyCH4IV ACGT 3 cut(s) 77, 222, 369
HpyCH4V TGCA 4 cut(s) 24, 69, 198, 219
HpyF10VI GCNNNNNNNGC 1 cut(s) 216
HpySE526I ACGT 3 cut(s) 77, 222, 369
Hsp92I GRCGYC 1 cut(s) 369
Hsp92II CATG 4 cut(s) 28, 73, 169, 219
Ksp22I TGATCA 1 cut(s) 72
Kzo9I GATC 3 cut(s) 72, 151, 243
LmnI GCTCC 2 cut(s) 4, 281
LpnPI CCDG 5 cut(s) 64, 96, 110, 254, 300
Lsp1109I GCAGC 1 cut(s) 8
MaeI CTAG 1 cut(s) 373
MaeII ACGT 3 cut(s) 77, 222, 369
MaeIII GTNAC 1 cut(s) 211
MalI GATC 3 cut(s) 74, 153, 245
MboI GATC 3 cut(s) 72, 151, 243
MboII GAAGA 2 cut(s) 145, 150
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 4 cut(s) 41, 86, 337, 351
MlyI GAGTC 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 182
MnlI CCTC 3 cut(s) 125, 165, 366
Mph1103I ATGCAT 1 cut(s) 71
MseI TTAA 1 cut(s) 39
MspI CCGG 2 cut(s) 51, 83
MspR9I CCNGG 3 cut(s) 51, 52, 83
MwoI GCNNNNNNNGC 1 cut(s) 216
NciI CCSGG 3 cut(s) 51, 52, 83
NdeII GATC 3 cut(s) 72, 151, 243
NlaIII CATG 4 cut(s) 28, 73, 169, 219
NlaIV GGNNCC 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 71
NspI RCATGY 1 cut(s) 219
PagI TCATGA 1 cut(s) 165
PfeI GAWTC 3 cut(s) 104, 182, 318
PkrI GCNGC 1 cut(s) 23
PleI GAGTC 1 cut(s) 245
PmaCI CACGTG 1 cut(s) 78
PmlI CACGTG 1 cut(s) 78
PpsI GAGTC 1 cut(s) 245
Ppu21I YACGTR 1 cut(s) 78
Psp1406I AACGTT 1 cut(s) 222
PspCI CACGTG 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 277
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 1 cut(s) 22
Sau3AI GATC 3 cut(s) 72, 151, 243
SchI GAGTC 1 cut(s) 246
ScrFI CCNGG 3 cut(s) 51, 52, 83
SduI GDGCHC 1 cut(s) 83
SetI ASST 7 cut(s) 9, 80, 225, 262, 289, 303, 372
SmaI CCCGGG 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
Sse9I AATT 4 cut(s) 41, 86, 337, 351
SsiI CCGC 1 cut(s) 112
SspMI CTAG 1 cut(s) 373
StyD4I CCNGG 3 cut(s) 49, 50, 81
TaiI ACGT 3 cut(s) 80, 225, 372
TaqI TCGA 1 cut(s) 185
TasI AATT 4 cut(s) 41, 86, 337, 351
TfiI GAWTC 3 cut(s) 104, 182, 318
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TseI GCWGC 1 cut(s) 21
TspDTI ATGAA 3 cut(s) 41, 182, 316
TspMI CCCGGG 1 cut(s) 50
XapI RAATTY 3 cut(s) 41, 86, 351
XceI RCATGY 1 cut(s) 219
XmaI CCCGGG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 14
XspI CTAG 1 cut(s) 373
ZraI GACGTC 1 cut(s) 370
Zsp2I ATGCAT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.