MD04G1157800.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008883
Physical Location & Seq
Reverse (-)
24589212 .. 24590986
1775 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1157800.v1.1.491

Sequence Viewer

Length: 1683 bp
ATGGAATCATTCCATCGATATCTGTTGTTGGGTTGGGGTTTCTTGCCACCATTCCACCAAAAGAGTCTTAAAACGTTGAACCTAGAAGCTCAAAAATTGGTAGGCTCCCTACCACCTTCCATTGGAAATCTTACTTACATCACAGGACTCAACTTGGGAAAAAACATATTTCATGGTGAAATTCCTCAAGAAATAGGTCGTCTAAGAAGCTTGCAATACCTCAACCTGTCTCGCAATTCTTTCAGCGGGAAAATTCCAACTAATATATCGCACTGTGCACGACTAAGAATGCTTAATCTTGAAGTCAATGGGATTATTGGGTCATTTCCAATCCAGCTTATTTCATTGTTGCGTTTAACACATTTGAGGCTTTCAAGTAACAATCTCACTGGAACCATCCGACCTTGGATGGGAAACTTTTCTTTGTTGAGTAGTCTTCACCTTGGCGAGAACAGATTTCAAGGAAGCATACCCAATGAGCTTGGGCACATAACAAGCTTGGAGGAGTTCGTAGTTGAGGTGAATGATCTATCTGTGTTGCTGAGAACCAATTGCATGGAGAGCTACCACCAAATCTCGGCACAATGCTTCCTAATCTCGTACAACTATACCGATTTGAACAAATTCCGAGGAAATATTCCTATATCATTGTCAAATGCTTCTAGACTTCAGACCCTTGAACTTGGTGAAAATGGCCTCTCTGGGACAGTCCCTGGTGAAAGTCTAGGAAGCTTGCGAAGCTTAGTTTGGCTAAACATTTATGGCAATCAATTAGGAAATAGAAAAGTTGGTGACTTGAATTTTCTTAGTTTCTTGGCTAATTGCACTAGATGGGAGATTTTGGCTCTTGACGGTAATAATTTTGGAGGAGGAATCCCGGGATCCATAGCCAACTTGTCGACCCAACTTAATATTCTTGGTCTAGGGTTAAACTTGATACATGGAAGCCTCCCTAACGGCATTGGAAACCTTATAAACTTGAATATTCTAGCATTAGACGATAACCATTTGGGTGGTAGTGTCCCGCTTGAAATTGGGAAGCTAAAGAAGTTAGAGCAACTGTATTTGGATGCTAATGAATTCTCTGGGTCAATCCCGTCTTCCCTTGGTTTGAGGGCACCATACCTATTAGATCTTTCCCTTTCCAGTAACAACCTTACGGGCACCATACCTAAAATGCTTATGGAGCTTTCAACTCTTTCAATTTCTTTAGACCTGTCTGATAATTATTTGACGGGTCTGATGCCCTTTGAGGTGGGTGATTTAGTGCATCTCACGGAGCTAAATGTATCAAGAAACAATTTATTAGGTGAAATCCCGCGCACCCTCGGCAGTTGTACTAGTTTGGAGCGCTTGTCTTTGCAAGGTAATAAGTTTGAAGGAACCATTCCACAATCTCTTAAGAATTTGAAAGGCTTGGAAAAACTTGATATTTCAAGCAACAACTTGTATGGGCCGATTCCTGAATTCATAGGCAAGCTTGGTGCTCTCAAGTATCTCAATCTTTCGTATAATGATTTTGAGGGCGAGCTGCCTAAATATGGTATTTTTGCAAATGCTAGTGGGGTTTTTGTTCTTGGAAATCATAGGCTCTGTGGTGGCATCCCACAATTACATCTACCTTCATGGCCCCCCCCCCCCCAAAACACCATTCATCTCGAGGACTACATTCCCCAAAAGTAG

Protein Analysis

561

Amino Acids

61.45

Weight (kDa)

6.54

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 50 - 105 4.8e-06 Leucine rich repeat
LRR_14 PF23598 63 - 191 1.9e-09 Leucine-rich repeat region
LRR_8 PF13855 403 - 459 6.9e-07 Leucine rich repeat
LRR_14 PF23598 404 - 500 1.1e-06 Leucine-rich repeat region
LRR_4 PF12799 447 - 489 9.4e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 448 - 507 8.5e-09 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 974
AccB1I GGYRCC 2 cut(s) 1117, 1163
AccI GTMKAC 1 cut(s) 899
AccII CGCG 1 cut(s) 1321
AciI CCGC 3 cut(s) 246, 1025, 1319
AclI AACGTT 1 cut(s) 74
AclWI GGATC 2 cut(s) 876, 889
AcsI RAATTY 7 cut(s) 180, 252, 623, 799, 1079, 1405, 1466
AcuI CTGAAG 1 cut(s) 653
AfaI GTAC 2 cut(s) 602, 1339
AfeI AGCGCT 1 cut(s) 1352
AfiI CCNNNNNNNGG 4 cut(s) 122, 410, 577, 1541
AflII CTTAAG 1 cut(s) 1400
AhlI ACTAGT 1 cut(s) 1340
AjnI CCWGG 1 cut(s) 712
AjuI GAANNNNNNNTTGG 6 cut(s) 730, 762, 897, 929, 1635, 1667
Alw21I GWGCWC 2 cut(s) 280, 1489
Alw26I GTCTC 1 cut(s) 234
Alw44I GTGCAC 1 cut(s) 276
AlwI GGATC 2 cut(s) 876, 889
AlwNI CAGNNNCTG 1 cut(s) 713
Ama87I CYCGRG 2 cut(s) 877, 1658
Aor51HI AGCGCT 1 cut(s) 1352
AoxI GGCC 3 cut(s) 694, 1454, 1628
ApaLI GTGCAC 1 cut(s) 276
ApeKI GCWGC 1 cut(s) 1531
ApoI RAATTY 7 cut(s) 180, 252, 623, 799, 1079, 1405, 1466
Asp700I GAANNNNTTC 2 cut(s) 418, 623
AspLEI GCGC 2 cut(s) 1323, 1353
AspS9I GGNCC 2 cut(s) 1454, 1629
AsuC2I CCSGG 2 cut(s) 878, 879
AsuHPI GGTGA 8 cut(s) 188, 431, 532, 698, 728, 803, 1271, 1322
AvaI CYCGRG 2 cut(s) 877, 1658
BaeGI GKGCMC 4 cut(s) 280, 489, 1120, 1166
BamHI GGATCC 1 cut(s) 881
BanI GGYRCC 2 cut(s) 1117, 1163
BbsI GAAGAC 2 cut(s) 428, 1092
Bbv12I GWGCWC 2 cut(s) 280, 1489
BbvI GCAGC 1 cut(s) 1518
BccI CCATC 4 cut(s) 21, 403, 404, 825
BceAI ACGGC 1 cut(s) 973
BciT130I CCWGG 1 cut(s) 714
BcnI CCSGG 2 cut(s) 878, 879
BcoDI GTCTC 1 cut(s) 234
BcuI ACTAGT 1 cut(s) 1340
BfaI CTAG 8 cut(s) 83, 663, 725, 828, 923, 989, 1341, 1560
BfoI RGCGCY 1 cut(s) 1354
BfrI CTTAAG 1 cut(s) 1400
BglII AGATCT 1 cut(s) 1132
BisI GCNGC 1 cut(s) 1532
BlsI GCNGC 1 cut(s) 1533
Bme1390I CCNGG 3 cut(s) 714, 878, 879
BmeT110I CYCGRG 2 cut(s) 877, 1658
BmgT120I GGNCC 2 cut(s) 1454, 1629
BmiI GGNNCC 7 cut(s) 106, 394, 883, 1119, 1165, 1384, 1631
BmrFI CCNGG 3 cut(s) 714, 878, 879
BmsI GCATC 4 cut(s) 1060, 1233, 1279, 1611
BpiI GAAGAC 2 cut(s) 428, 1092
BpuEI CTTGAG 2 cut(s) 171, 1475
BpuMI CCSGG 2 cut(s) 878, 879
Bsa29I ATCGAT 1 cut(s) 16
BsaJI CCNNGG 7 cut(s) 404, 442, 628, 712, 877, 1105, 1327
BsaXI ACNNNNNCTCC 2 cut(s) 1340, 1370
Bsc4I CCNNNNNNNGG 4 cut(s) 122, 410, 577, 1541
Bse1I ACTGG 2 cut(s) 394, 1146
BseBI CCWGG 1 cut(s) 714
BseCI ATCGAT 1 cut(s) 16
BseDI CCNNGG 7 cut(s) 404, 442, 628, 712, 877, 1105, 1327
BseGI GGATG 4 cut(s) 396, 414, 1075, 1602
BseLI CCNNNNNNNGG 4 cut(s) 122, 410, 577, 1541
BseMII CTCAG 1 cut(s) 533
BseNI ACTGG 2 cut(s) 394, 1146
BseRI GAGGAG 2 cut(s) 518, 882
BseSI GKGCMC 4 cut(s) 280, 489, 1120, 1166
BseXI GCAGC 1 cut(s) 1518
Bsh1236I CGCG 1 cut(s) 1321
BshFI GGCC 3 cut(s) 696, 1456, 1630
BshNI GGYRCC 2 cut(s) 1117, 1163
BshVI ATCGAT 1 cut(s) 16
BsiHKAI GWGCWC 2 cut(s) 280, 1489
BsiHKCI CYCGRG 2 cut(s) 877, 1658
BsiSI CCGG 1 cut(s) 878
BslFI GGGAC 3 cut(s) 695, 718, 1007
BslI CCNNNNNNNGG 4 cut(s) 122, 410, 577, 1541
BsmAI GTCTC 1 cut(s) 234
BsmFI GGGAC 3 cut(s) 695, 718, 1007
BsmI GAATGC 1 cut(s) 294
BsnI GGCC 3 cut(s) 696, 1456, 1630
BsoBI CYCGRG 2 cut(s) 877, 1658
Bsp1286I GDGCHC 5 cut(s) 280, 489, 1120, 1166, 1489
Bsp143I GATC 3 cut(s) 526, 881, 1132
BspACI CCGC 3 cut(s) 246, 1025, 1319
BspANI GGCC 3 cut(s) 696, 1456, 1630
BspCNI CTCAG 1 cut(s) 534
BspDI ATCGAT 1 cut(s) 16
BspFNI CGCG 1 cut(s) 1321
BspLI GGNNCC 7 cut(s) 106, 394, 883, 1119, 1165, 1384, 1631
BspPI GGATC 2 cut(s) 876, 889
BspT107I GGYRCC 2 cut(s) 1117, 1163
BspTI CTTAAG 1 cut(s) 1400
BsrI ACTGG 2 cut(s) 394, 1146
BssECI CCNNGG 7 cut(s) 404, 442, 628, 712, 877, 1105, 1327
BssMI GATC 3 cut(s) 526, 881, 1132
BssT1I CCWWGG 3 cut(s) 404, 442, 1105
Bst2UI CCWGG 1 cut(s) 714
Bst4CI ACNGT 4 cut(s) 275, 709, 854, 1062
BstAFI CTTAAG 1 cut(s) 1400
BstC8I GCNNGC 4 cut(s) 212, 734, 1478, 1529
BstDEI CTNAG 5 cut(s) 203, 284, 542, 742, 806
BstF5I GGATG 4 cut(s) 396, 414, 1075, 1602
BstFNI CGCG 1 cut(s) 1321
BstH2I RGCGCY 1 cut(s) 1354
BstHHI GCGC 2 cut(s) 1323, 1353
BstKTI GATC 3 cut(s) 529, 884, 1135
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 3 cut(s) 526, 881, 1132
BstMWI GCNNNNNNNGC 4 cut(s) 561, 738, 1186, 1329
BstNI CCWGG 1 cut(s) 714
BstSCI CCNGG 3 cut(s) 712, 876, 877
BstSLI GKGCMC 4 cut(s) 280, 489, 1120, 1166
BstUI CGCG 1 cut(s) 1321
BstV1I GCAGC 1 cut(s) 1518
BstV2I GAAGAC 2 cut(s) 428, 1092
BstX2I RGATCY 2 cut(s) 881, 1132
BstXI CCANNNNNNTGG 2 cut(s) 556, 1013
BstYI RGATCY 2 cut(s) 881, 1132
Bsu15I ATCGAT 1 cut(s) 16
BsuRI GGCC 3 cut(s) 696, 1456, 1630
BsuTUI ATCGAT 1 cut(s) 16
BtsCI GGATG 4 cut(s) 396, 414, 1075, 1602
BtsIMutI CAGTG 2 cut(s) 271, 387
Cac8I GCNNGC 4 cut(s) 212, 734, 1478, 1529
CaiI CAGNNNCTG 1 cut(s) 713
CfoI GCGC 2 cut(s) 1323, 1353
Cfr13I GGNCC 2 cut(s) 1454, 1629
Cfr9I CCCGGG 1 cut(s) 877
ClaI ATCGAT 1 cut(s) 16
Csp6I GTAC 2 cut(s) 601, 1338
CviAII CATG 4 cut(s) 173, 556, 941, 1626
CviQI GTAC 2 cut(s) 601, 1338
DdeI CTNAG 5 cut(s) 203, 284, 542, 742, 806
DpnI GATC 3 cut(s) 528, 883, 1134
DpnII GATC 3 cut(s) 526, 881, 1132
Eco130I CCWWGG 3 cut(s) 404, 442, 1105
Eco32I GATATC 1 cut(s) 20
Eco47III AGCGCT 1 cut(s) 1352
Eco57I CTGAAG 1 cut(s) 653
Eco88I CYCGRG 2 cut(s) 877, 1658
EcoRI GAATTC 2 cut(s) 1079, 1466
EcoRII CCWGG 1 cut(s) 712
EcoRV GATATC 1 cut(s) 20
EcoT14I CCWWGG 3 cut(s) 404, 442, 1105
ErhI CCWWGG 3 cut(s) 404, 442, 1105
FaeI CATG 4 cut(s) 176, 559, 944, 1629
FaqI GGGAC 3 cut(s) 695, 718, 1007
FatI CATG 4 cut(s) 172, 555, 940, 1625
FauI CCCGC 3 cut(s) 239, 1032, 1326
FblI GTMKAC 1 cut(s) 899
Fnu4HI GCNGC 1 cut(s) 1532
FokI GGATG 4 cut(s) 383, 421, 1082, 1589
Fsp4HI GCNGC 1 cut(s) 1532
FspBI CTAG 8 cut(s) 83, 663, 725, 828, 923, 989, 1341, 1560
GlaI GCGC 2 cut(s) 1322, 1352
GluI GCNGC 1 cut(s) 1532
HaeII RGCGCY 1 cut(s) 1354
HaeIII GGCC 3 cut(s) 696, 1456, 1630
HapII CCGG 1 cut(s) 878
HhaI GCGC 2 cut(s) 1323, 1353
Hin1II CATG 4 cut(s) 176, 559, 944, 1629
Hin6I GCGC 2 cut(s) 1321, 1351
HinP1I GCGC 2 cut(s) 1321, 1351
HincII GTYRAC 1 cut(s) 900
HindII GTYRAC 1 cut(s) 900
HindIII AAGCTT 5 cut(s) 208, 496, 730, 739, 1478
HinfI GANTC 5 cut(s) 5, 64, 147, 873, 1459
HpaII CCGG 1 cut(s) 878
HphI GGTGA 8 cut(s) 188, 431, 532, 698, 728, 803, 1271, 1322
Hpy166II GTNNAC 2 cut(s) 278, 900
Hpy188I TCNGA 5 cut(s) 401, 629, 672, 1222, 1242
Hpy188III TCNNGA 7 cut(s) 188, 299, 663, 848, 1293, 1463, 1658
Hpy8I GTNNAC 2 cut(s) 278, 900
HpyAV CCTTC 3 cut(s) 126, 1373, 1632
HpyCH4III ACNGT 4 cut(s) 275, 709, 854, 1062
HpyCH4IV ACGT 1 cut(s) 74
HpyCH4V TGCA 7 cut(s) 214, 278, 555, 825, 1270, 1363, 1553
HpyF10VI GCNNNNNNNGC 4 cut(s) 561, 738, 1186, 1329
HpyF3I CTNAG 5 cut(s) 203, 284, 542, 742, 806
HpySE526I ACGT 1 cut(s) 74
Hsp92II CATG 4 cut(s) 176, 559, 944, 1629
HspAI GCGC 2 cut(s) 1321, 1351
Kzo9I GATC 3 cut(s) 526, 881, 1132
LmnI GCTCC 4 cut(s) 110, 1186, 1279, 1348
Lsp1109I GCAGC 1 cut(s) 1518
LweI GCATC 4 cut(s) 1060, 1233, 1279, 1611
MaeI CTAG 8 cut(s) 83, 663, 725, 828, 923, 989, 1341, 1560
MaeII ACGT 1 cut(s) 74
MaeIII GTNAC 3 cut(s) 377, 791, 1148
MalI GATC 3 cut(s) 528, 883, 1134
MboI GATC 3 cut(s) 526, 881, 1132
MboII GAAGA 2 cut(s) 428, 1092
MfeI CAATTG 1 cut(s) 550
MflI RGATCY 2 cut(s) 881, 1132
MhlI GDGCHC 5 cut(s) 280, 489, 1120, 1166, 1489
MlyI GAGTC 2 cut(s) 73, 141
MmeI TCCRAC 2 cut(s) 281, 424
MroXI GAANNNNTTC 2 cut(s) 418, 623
MseI TTAA 6 cut(s) 69, 294, 356, 909, 929, 1401
MslI CAYNNNNRTG 1 cut(s) 1011
MspA1I CMGCKG 1 cut(s) 246
MspCI CTTAAG 1 cut(s) 1400
MspI CCGG 1 cut(s) 878
MspR9I CCNGG 3 cut(s) 714, 878, 879
MunI CAATTG 1 cut(s) 550
Mva1269I GAATGC 1 cut(s) 294
MvaI CCWGG 1 cut(s) 714
MvnI CGCG 1 cut(s) 1321
MwoI GCNNNNNNNGC 4 cut(s) 561, 738, 1186, 1329
NciI CCSGG 2 cut(s) 878, 879
NdeII GATC 3 cut(s) 526, 881, 1132
NlaIII CATG 4 cut(s) 176, 559, 944, 1629
NlaIV GGNNCC 7 cut(s) 106, 394, 883, 1119, 1165, 1384, 1631
NmeAIII GCCGAG 2 cut(s) 557, 1308
NmuCI GTSAC 1 cut(s) 791
PaeR7I CTCGAG 1 cut(s) 1658
PctI GAATGC 1 cut(s) 294
PdmI GAANNNNTTC 2 cut(s) 418, 623
PfeI GAWTC 3 cut(s) 5, 873, 1459
PkrI GCNGC 1 cut(s) 1533
PleI GAGTC 2 cut(s) 72, 141
PpsI GAGTC 2 cut(s) 72, 141
PsiI TTATAA 1 cut(s) 974
Psp1406I AACGTT 1 cut(s) 74
Psp6I CCWGG 1 cut(s) 712
PspGI CCWGG 1 cut(s) 712
PspN4I GGNNCC 7 cut(s) 106, 394, 883, 1119, 1165, 1384, 1631
PspPI GGNCC 2 cut(s) 1454, 1629
PstNI CAGNNNCTG 1 cut(s) 713
PsuI RGATCY 2 cut(s) 881, 1132
RsaI GTAC 2 cut(s) 602, 1339
RsaNI GTAC 2 cut(s) 601, 1338
RseI CAYNNNNRTG 1 cut(s) 1011
SalI GTCGAC 1 cut(s) 898
SaqAI TTAA 6 cut(s) 69, 294, 356, 909, 929, 1401
SatI GCNGC 1 cut(s) 1532
Sau3AI GATC 3 cut(s) 526, 881, 1132
Sau96I GGNCC 2 cut(s) 1454, 1629
SchI GAGTC 2 cut(s) 73, 141
ScrFI CCNGG 3 cut(s) 714, 878, 879
SduI GDGCHC 5 cut(s) 280, 489, 1120, 1166, 1489
SfaNI GCATC 4 cut(s) 1060, 1233, 1279, 1611
Sfr274I CTCGAG 1 cut(s) 1658
SlaI CTCGAG 1 cut(s) 1658
SmaI CCCGGG 1 cut(s) 879
SmiMI CAYNNNNRTG 1 cut(s) 1011
SmlI CTYRAG 4 cut(s) 186, 1400, 1490, 1658
SmoI CTYRAG 4 cut(s) 186, 1400, 1490, 1658
SpeI ACTAGT 1 cut(s) 1340
SsiI CCGC 3 cut(s) 246, 1025, 1319
SspI AATATT 3 cut(s) 637, 913, 985
SspMI CTAG 8 cut(s) 83, 663, 725, 828, 923, 989, 1341, 1560
StyD4I CCNGG 3 cut(s) 712, 876, 877
StyI CCWWGG 3 cut(s) 404, 442, 1105
TaaI ACNGT 4 cut(s) 275, 709, 854, 1062
TaiI ACGT 1 cut(s) 77
TaqI TCGA 3 cut(s) 16, 899, 1659
TatI WGTACW 1 cut(s) 1337
TfiI GAWTC 3 cut(s) 5, 873, 1459
Tru1I TTAA 6 cut(s) 69, 294, 356, 909, 929, 1401
Tru9I TTAA 6 cut(s) 69, 294, 356, 909, 929, 1401
TscAI CASTG 2 cut(s) 278, 394
TseFI GTSAC 1 cut(s) 791
TseI GCWGC 1 cut(s) 1531
Tsp45I GTSAC 1 cut(s) 791
TspDTI ATGAA 6 cut(s) 161, 333, 1092, 1459, 1614, 1643
TspGWI ACGGA 1 cut(s) 1292
TspMI CCCGGG 1 cut(s) 877
TspRI CASTG 2 cut(s) 278, 394
Vha464I CTTAAG 1 cut(s) 1400
VneI GTGCAC 1 cut(s) 276
XapI RAATTY 7 cut(s) 180, 252, 623, 799, 1079, 1405, 1466
XbaI TCTAGA 1 cut(s) 662
XhoI CTCGAG 1 cut(s) 1658
XmaI CCCGGG 1 cut(s) 877
XmiI GTMKAC 1 cut(s) 899
XmnI GAANNNNTTC 2 cut(s) 418, 623
XspI CTAG 8 cut(s) 83, 663, 725, 828, 923, 989, 1341, 1560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.