MD04G1158700.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008883
Physical Location & Seq
Reverse (-)
24649811 .. 24650275
465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1158700.v1.1.491

Sequence Viewer

Length: 465 bp
ATGATGGAGCATTCACGTACCAATCGTAAGCTGGTTTTGTTCAAATTCTTGCATGGGTTCATTCTTTTATGCATGACCACATGCCTTCAATCTGCAGCACTTCGTAGCCTTACTATGCTTGGAAATGAATCTGATCACTTGGCACTGCTAGACTTCAAGAAAAGAATAACCGATGATCCCTTTGATGTCATGAGCTCGTGGAATCATTCCATCCATATCTGCAGTTGGGTTGGGGTTTCTTGCCACCGTTCCACCAAAAGAGTCTTGATGTTGAACCTGAAATCTAGAAAATTGGTAGGCTCCATTCCACCTTCAGTTGGAAATCTTACTTATCTTACTGCAATCAATTTGAGAGACAACAATTTTCATGGGAAAATTCCTCTAGAAATGGGTCGACTACTAAGCCTACAATATATCAACCTTTCTCATAATTCCTTCCGTGGGAAATTTCCAACTAATTTGTAG

Protein Analysis

155

Amino Acids

17.58

Weight (kDa)

9.97

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 43 - 81 2.4e-11 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 394
AclWI GGATC 1 cut(s) 170
AcsI RAATTY 3 cut(s) 44, 375, 446
AcuI CTGAAG 1 cut(s) 297
AfaI GTAC 1 cut(s) 19
AfiI CCNNNNNNNGG 2 cut(s) 317, 441
AgsI TTSAA 4 cut(s) 43, 89, 157, 274
AluBI AGCT 2 cut(s) 31, 195
AluI AGCT 2 cut(s) 31, 195
Alw21I GWGCWC 1 cut(s) 197
Alw26I GTCTC 1 cut(s) 348
AlwI GGATC 1 cut(s) 170
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 3 cut(s) 44, 375, 446
BanII GRGCYC 1 cut(s) 197
BauI CACGAG 1 cut(s) 196
Bbv12I GWGCWC 1 cut(s) 197
BbvI GCAGC 1 cut(s) 107
BccI CCATC 1 cut(s) 218
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 348
BfaI CTAG 3 cut(s) 149, 285, 383
BfmI CTRYAG 2 cut(s) 93, 220
BisI GCNGC 1 cut(s) 96
BlsI GCNGC 1 cut(s) 97
BmiI GGNNCC 1 cut(s) 301
BsaAI YACGTR 1 cut(s) 17
BsaJI CCNNGG 1 cut(s) 439
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 441
BseDI CCNNGG 1 cut(s) 439
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 2 cut(s) 317, 441
BseXI GCAGC 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 197
BslI CCNNNNNNNGG 2 cut(s) 317, 441
BsmAI GTCTC 1 cut(s) 348
BsmI GAATGC 1 cut(s) 10
Bsp1286I GDGCHC 1 cut(s) 197
Bsp143I GATC 2 cut(s) 133, 175
BspHI TCATGA 1 cut(s) 189
BspLI GGNNCC 1 cut(s) 301
BspMAI CTGCAG 2 cut(s) 97, 224
BspPI GGATC 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 439
BssMI GATC 2 cut(s) 133, 175
BssSI CACGAG 1 cut(s) 196
Bst2BI CACGAG 1 cut(s) 196
Bst4CI ACNGT 1 cut(s) 248
BstBAI YACGTR 1 cut(s) 17
BstDEI CTNAG 1 cut(s) 401
BstDSI CCRYGG 1 cut(s) 439
BstF5I GGATG 1 cut(s) 210
BstKTI GATC 2 cut(s) 136, 178
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 2 cut(s) 133, 175
BstNSI RCATGY 1 cut(s) 84
BstSFI CTRYAG 2 cut(s) 93, 220
BstV1I GCAGC 1 cut(s) 107
BtgI CCRYGG 1 cut(s) 439
BtsCI GGATG 1 cut(s) 210
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 1 cut(s) 143
CciI TCATGA 1 cut(s) 189
Csp6I GTAC 1 cut(s) 18
CviAII CATG 5 cut(s) 53, 73, 81, 190, 368
CviJI RGCY 5 cut(s) 31, 108, 195, 300, 405
CviKI_1 RGCY 5 cut(s) 31, 108, 195, 300, 405
CviQI GTAC 1 cut(s) 18
DdeI CTNAG 1 cut(s) 401
DpnI GATC 2 cut(s) 135, 177
DpnII GATC 2 cut(s) 133, 175
Ecl136II GAGCTC 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 197
Eco53kI GAGCTC 1 cut(s) 195
Eco57I CTGAAG 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 74
EcoT38I GRGCYC 1 cut(s) 197
FaeI CATG 5 cut(s) 56, 76, 84, 193, 371
FatI CATG 5 cut(s) 52, 72, 80, 189, 367
FbaI TGATCA 1 cut(s) 133
FblI GTMKAC 1 cut(s) 394
Fnu4HI GCNGC 1 cut(s) 96
FokI GGATG 1 cut(s) 197
FriOI GRGCYC 1 cut(s) 197
Fsp4HI GCNGC 1 cut(s) 96
FspBI CTAG 3 cut(s) 149, 285, 383
GluI GCNGC 1 cut(s) 96
Hin1II CATG 5 cut(s) 56, 76, 84, 193, 371
HincII GTYRAC 1 cut(s) 395
HindII GTYRAC 1 cut(s) 395
HinfI GANTC 3 cut(s) 128, 202, 261
Hpy166II GTNNAC 1 cut(s) 395
Hpy188I TCNGA 1 cut(s) 133
Hpy188III TCNNGA 5 cut(s) 157, 190, 265, 285, 383
Hpy8I GTNNAC 1 cut(s) 395
HpyAV CCTTC 3 cut(s) 95, 321, 445
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 5 cut(s) 52, 72, 95, 222, 341
HpyF3I CTNAG 1 cut(s) 401
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 5 cut(s) 56, 76, 84, 193, 371
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 2 cut(s) 133, 175
LmnI GCTCC 2 cut(s) 7, 305
LpnPI CCDG 2 cut(s) 17, 290
Lsp1109I GCAGC 1 cut(s) 107
MaeI CTAG 3 cut(s) 149, 285, 383
MaeII ACGT 1 cut(s) 16
MalI GATC 2 cut(s) 135, 177
MboI GATC 2 cut(s) 133, 175
MhlI GDGCHC 1 cut(s) 197
MluCI AATT 8 cut(s) 44, 290, 346, 361, 375, 430, 446, 457
MlyI GAGTC 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 298
MnlI CCTC 1 cut(s) 390
Mph1103I ATGCAT 1 cut(s) 74
Mva1269I GAATGC 1 cut(s) 10
NdeII GATC 2 cut(s) 133, 175
NlaIII CATG 5 cut(s) 56, 76, 84, 193, 371
NlaIV GGNNCC 1 cut(s) 301
NsiI ATGCAT 1 cut(s) 74
NspI RCATGY 1 cut(s) 84
PagI TCATGA 1 cut(s) 189
PcsI WCGNNNNNNNCGW 1 cut(s) 22
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 128, 202
PkrI GCNGC 1 cut(s) 97
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
Ppu21I YACGTR 1 cut(s) 17
Psp124BI GAGCTC 1 cut(s) 197
PspN4I GGNNCC 1 cut(s) 301
PstI CTGCAG 2 cut(s) 97, 224
RsaI GTAC 1 cut(s) 19
RsaNI GTAC 1 cut(s) 18
SacI GAGCTC 1 cut(s) 197
SalI GTCGAC 1 cut(s) 393
SatI GCNGC 1 cut(s) 96
Sau3AI GATC 2 cut(s) 133, 175
SchI GAGTC 1 cut(s) 270
SduI GDGCHC 1 cut(s) 197
SetI ASST 6 cut(s) 19, 33, 197, 279, 313, 423
SfcI CTRYAG 2 cut(s) 93, 220
Sse9I AATT 8 cut(s) 44, 290, 346, 361, 375, 430, 446, 457
SspMI CTAG 3 cut(s) 149, 285, 383
SstI GAGCTC 1 cut(s) 197
TaaI ACNGT 1 cut(s) 248
TaiI ACGT 1 cut(s) 19
TaqI TCGA 1 cut(s) 394
TasI AATT 8 cut(s) 44, 290, 346, 361, 375, 430, 446, 457
TfiI GAWTC 2 cut(s) 128, 202
TscAI CASTG 1 cut(s) 150
TseI GCWGC 1 cut(s) 95
TspDTI ATGAA 3 cut(s) 49, 141, 356
TspGWI ACGGA 1 cut(s) 428
TspRI CASTG 1 cut(s) 150
XapI RAATTY 3 cut(s) 44, 375, 446
XbaI TCTAGA 2 cut(s) 284, 382
XceI RCATGY 1 cut(s) 84
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
XmiI GTMKAC 1 cut(s) 394
XspI CTAG 3 cut(s) 149, 285, 383
Zsp2I ATGCAT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.