MD12G1130600.v1.1

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008883
Physical Location & Seq
Forward (+)
20571872 .. 20573191
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1130600.v1.1.491

Sequence Viewer

Length: 1320 bp
ATGGAGCATGCACGTACTACTGGAGGCAGGCTGAGTTTGGTTAAACTCTTTCATGGCTTCATTCTTTTATGCATGAGCACGCATCTGGGATCTGCGGCACTCTCCAGCACTAGTCTTGGAAATGAAACTGATCGCATGGTGCTACTCAAGTTCAAGAAAAGCATCACTCAAGATCCTCTCCATGTCATGAGCTCATGGAATGAATCCGTCCATTTCTGCGGTTGGGTAGGCGTTACGTGCAACCATTACACCAGAAGAGTCTTCATGTTGAACCTGCAAGATCAGAAATTGACAGGCTCTTTACCTCCTTCTATAGGAAACCTTTCCTATCTTGCTAGAATCAACCTAGGAAGCAACAGCTTTCATGGTGAAATTCCTGAAGAAATTGGTCGTCTGCAAAGCTTGCACTTTCTCAACTTGTCTTACAATTCCTTCAGCGGGAAAATTCCAACCAATTTATCCCAGTGTACACAACTAAAGACGATTGAGTTTCGTTACAATGAGGTTATTGGGTCAATTCCAAACCAACTCAGTTCCTTGTTGAAATTAAATCATATGCGGCTTTCTAATAACAATCTCACTGGAACCGTCCCTGTTTGGATTGGAAACTTTTCTTCTCTGCATCATTTTGATCTTGGCTATAACAATTTTCAAGGAAGCATACCCAATGAGCTCGGGCGGCTAACAAGAATGCAAAAACTCTCATTTATAGGAAACAATCTCTCTGGTACAATCCCTGCTTCAATCTATAATATTTCTTCCATATACTTGTTAATAGTTGGTCAAAACCAACTGCATGGAGAGCTACCGCCAAATGTTGGCATTACTCTTCCTAATCTCAAAAAATTTGTCGGTGGCAAAAACAAATTCACAGGAAATATTCCTCCATCATTGTCAAATGCTTCTAGATTGCAACTTTTTGACGTTGCTAAAAATGATCTTACTGGAACACTCCCTGCTCTTGGATCCTTACAAAGCTTAGTTTGGCTAAACCTTTATAGCAATAGACTGGGAAGTGGGAAAGCTGGCGATCTTAATTTTCTCAAATTCTTGGCTAACTGTACTAGTCTTGAGTTGTTGATTCTTACCGACAATCAATTTGGAGGAGAATTGCCTGTGTCCATTTCCAACCTTTCTACCACACTAGGAAAATCTTTTTCTGGGGGGAAATTTGATACATGGAAGCATCACTTTGGGTATTGGAAATCTTGTAAATTTGGTCAGTTTATGGAAATGGAGGCTAACTATTTAAGTGGTAGTCTTCCTGAAGAAATTGGGAGGCTTCAGAAGTTGAAAAAATTATATTTTGGGCGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase RVT_2

Protein Analysis

440

Amino Acids

48.67

Weight (kDa)

9.5

Isoelectric Point (pI)

29.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 42 - 81 9.8e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 128 - 336 2e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 282
AccB7I CCANNNNNTGG 1 cut(s) 818
AciI CCGC 7 cut(s) 95, 219, 438, 559, 679, 809, 1312
AclWI GGATC 4 cut(s) 97, 167, 960, 973
AcsI RAATTY 7 cut(s) 372, 444, 845, 866, 1046, 1169, 1214
AcuI CTGAAG 4 cut(s) 399, 418, 1268, 1287
AfaI GTAC 4 cut(s) 16, 469, 730, 1063
AfiI CCNNNNNNNGG 4 cut(s) 314, 438, 818, 962
AgsI TTSAA 6 cut(s) 154, 271, 544, 653, 744, 1294
AhlI ACTAGT 2 cut(s) 110, 1064
AluBI AGCT 7 cut(s) 192, 360, 402, 673, 805, 978, 1025
AluI AGCT 7 cut(s) 192, 360, 402, 673, 805, 978, 1025
Alw21I GWGCWC 3 cut(s) 80, 194, 675
AlwI GGATC 4 cut(s) 97, 167, 960, 973
Ama87I CYCGRG 1 cut(s) 674
ApoI RAATTY 7 cut(s) 372, 444, 845, 866, 1046, 1169, 1214
ArsI GACNNNNNNTTYG 4 cut(s) 834, 866, 1082, 1114
Asp700I GAANNNNTTC 2 cut(s) 322, 610
AspA2I CCTAGG 1 cut(s) 346
AsuHPI GGTGA 1 cut(s) 380
AvaI CYCGRG 1 cut(s) 674
AvrII CCTAGG 1 cut(s) 346
BamHI GGATCC 1 cut(s) 965
BanII GRGCYC 2 cut(s) 194, 675
BbsI GAAGAC 2 cut(s) 253, 1253
Bbv12I GWGCWC 3 cut(s) 80, 194, 675
BccI CCATC 1 cut(s) 895
BcuI ACTAGT 2 cut(s) 110, 1064
BfaI CTAG 6 cut(s) 111, 336, 347, 906, 1065, 1145
BfmI CTRYAG 1 cut(s) 312
BfuAI ACCTGC 1 cut(s) 282
BisI GCNGC 4 cut(s) 96, 560, 680, 1313
BlnI CCTAGG 1 cut(s) 346
BlsI GCNGC 4 cut(s) 97, 561, 681, 1314
BmeT110I CYCGRG 1 cut(s) 674
BmiI GGNNCC 2 cut(s) 586, 967
BmrI ACTGGG 2 cut(s) 457, 1019
BmsI GCATC 4 cut(s) 91, 171, 631, 1195
BmuI ACTGGG 2 cut(s) 457, 1019
BpiI GAAGAC 2 cut(s) 253, 1253
BpmI CTGGAG 2 cut(s) 42, 88
BpuEI CTTGAG 3 cut(s) 131, 153, 1091
BsaAI YACGTR 2 cut(s) 14, 237
BsaJI CCNNGG 1 cut(s) 346
Bsc4I CCNNNNNNNGG 4 cut(s) 314, 438, 818, 962
Bse1I ACTGG 5 cut(s) 25, 463, 586, 949, 1014
BseDI CCNNGG 1 cut(s) 346
BseLI CCNNNNNNNGG 4 cut(s) 314, 438, 818, 962
BseMII CTCAG 2 cut(s) 23, 544
BseNI ACTGG 5 cut(s) 25, 463, 586, 949, 1014
BseRI GAGGAG 1 cut(s) 1119
BsiHKAI GWGCWC 3 cut(s) 80, 194, 675
BsiHKCI CYCGRG 1 cut(s) 674
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 4 cut(s) 314, 438, 818, 962
BsmFI GGGAC 1 cut(s) 575
BsmI GAATGC 1 cut(s) 696
BsoBI CYCGRG 1 cut(s) 674
Bsp1286I GDGCHC 3 cut(s) 80, 194, 675
Bsp1407I TGTACA 1 cut(s) 467
Bsp143I GATC 8 cut(s) 89, 130, 172, 280, 631, 937, 965, 1030
BspACI CCGC 7 cut(s) 95, 219, 438, 559, 679, 809, 1312
BspCNI CTCAG 2 cut(s) 24, 543
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 2 cut(s) 586, 967
BspMI ACCTGC 1 cut(s) 282
BspPI GGATC 4 cut(s) 97, 167, 960, 973
BsrGI TGTACA 1 cut(s) 467
BsrI ACTGG 5 cut(s) 25, 463, 586, 949, 1014
BssECI CCNNGG 1 cut(s) 346
BssMI GATC 8 cut(s) 89, 130, 172, 280, 631, 937, 965, 1030
BssT1I CCWWGG 1 cut(s) 346
Bst4CI ACNGT 2 cut(s) 589, 1061
Bst6I CTCTTC 2 cut(s) 250, 834
BstAPI GCANNNNNTGC 1 cut(s) 403
BstAUI TGTACA 1 cut(s) 467
BstBAI YACGTR 2 cut(s) 14, 237
BstC8I GCNNGC 5 cut(s) 9, 29, 80, 404, 1027
BstDEI CTNAG 3 cut(s) 32, 530, 979
BstENI CCTNNNNNAGG 1 cut(s) 312
BstKTI GATC 8 cut(s) 92, 133, 175, 283, 634, 940, 968, 1033
BstMBI GATC 8 cut(s) 89, 130, 172, 280, 631, 937, 965, 1030
BstMWI GCNNNNNNNGC 4 cut(s) 237, 403, 679, 802
BstNSI RCATGY 1 cut(s) 11
BstSFI CTRYAG 1 cut(s) 312
BstV2I GAAGAC 2 cut(s) 253, 1253
BstX2I RGATCY 3 cut(s) 89, 172, 965
BstXI CCANNNNNNTGG 1 cut(s) 797
BstYI RGATCY 3 cut(s) 89, 172, 965
BtsIMutI CAGTG 2 cut(s) 470, 579
BveI ACCTGC 1 cut(s) 282
Cac8I GCNNGC 5 cut(s) 9, 29, 80, 404, 1027
CciI TCATGA 1 cut(s) 186
Csp6I GTAC 4 cut(s) 15, 468, 729, 1062
CviQI GTAC 4 cut(s) 15, 468, 729, 1062
DdeI CTNAG 3 cut(s) 32, 530, 979
DpnI GATC 8 cut(s) 91, 132, 174, 282, 633, 939, 967, 1032
DpnII GATC 8 cut(s) 89, 130, 172, 280, 631, 937, 965, 1030
Eam1104I CTCTTC 2 cut(s) 250, 834
EarI CTCTTC 2 cut(s) 250, 834
Ecl136II GAGCTC 2 cut(s) 192, 673
Eco130I CCWWGG 1 cut(s) 346
Eco24I GRGCYC 2 cut(s) 194, 675
Eco53kI GAGCTC 2 cut(s) 192, 673
Eco57I CTGAAG 4 cut(s) 399, 418, 1268, 1287
Eco88I CYCGRG 1 cut(s) 674
EcoICRI GAGCTC 2 cut(s) 192, 673
EcoNI CCTNNNNNAGG 1 cut(s) 312
EcoT14I CCWWGG 1 cut(s) 346
EcoT22I ATGCAT 1 cut(s) 74
EcoT38I GRGCYC 2 cut(s) 194, 675
ErhI CCWWGG 1 cut(s) 346
FalI AAGNNNNNCTT 2 cut(s) 1175, 1207
FaqI GGGAC 1 cut(s) 575
FauI CCCGC 1 cut(s) 431
FauNDI CATATG 1 cut(s) 555
Fnu4HI GCNGC 4 cut(s) 96, 560, 680, 1313
FriOI GRGCYC 2 cut(s) 194, 675
Fsp4HI GCNGC 4 cut(s) 96, 560, 680, 1313
FspBI CTAG 6 cut(s) 111, 336, 347, 906, 1065, 1145
GluI GCNGC 4 cut(s) 96, 560, 680, 1313
GsuI CTGGAG 2 cut(s) 42, 88
HindIII AAGCTT 2 cut(s) 400, 976
HinfI GANTC 4 cut(s) 203, 258, 339, 1081
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 2 cut(s) 468, 470
Hpy188I TCNGA 2 cut(s) 285, 1287
Hpy188III TCNNGA 7 cut(s) 154, 170, 187, 377, 906, 1070, 1265
Hpy8I GTNNAC 2 cut(s) 468, 470
HpyAV CCTTC 2 cut(s) 318, 442
HpyCH4III ACNGT 2 cut(s) 589, 1061
HpyCH4IV ACGT 3 cut(s) 13, 236, 924
HpyF10VI GCNNNNNNNGC 4 cut(s) 237, 403, 679, 802
HpyF3I CTNAG 3 cut(s) 32, 530, 979
HpySE526I ACGT 3 cut(s) 13, 236, 924
Kzo9I GATC 8 cut(s) 89, 130, 172, 280, 631, 937, 965, 1030
LmnI GCTCC 1 cut(s) 4
LweI GCATC 4 cut(s) 91, 171, 631, 1195
MaeI CTAG 6 cut(s) 111, 336, 347, 906, 1065, 1145
MaeII ACGT 3 cut(s) 13, 236, 924
MaeIII GTNAC 2 cut(s) 232, 494
MalI GATC 8 cut(s) 91, 132, 174, 282, 633, 939, 967, 1032
MboI GATC 8 cut(s) 89, 130, 172, 280, 631, 937, 965, 1030
MboII GAAGA 8 cut(s) 253, 267, 392, 606, 750, 821, 1253, 1280
MflI RGATCY 3 cut(s) 89, 172, 965
MhlI GDGCHC 3 cut(s) 80, 194, 675
MlyI GAGTC 1 cut(s) 267
MmeI TCCRAC 2 cut(s) 473, 1152
MnlI CCTC 8 cut(s) 17, 186, 315, 496, 894, 1097, 1231, 1272
Mph1103I ATGCAT 1 cut(s) 74
MroXI GAANNNNTTC 2 cut(s) 322, 610
MseI TTAA 5 cut(s) 42, 548, 773, 1035, 1250
MspA1I CMGCKG 1 cut(s) 438
Mva1269I GAATGC 1 cut(s) 696
MwoI GCNNNNNNNGC 4 cut(s) 237, 403, 679, 802
NdeI CATATG 1 cut(s) 555
NdeII GATC 8 cut(s) 89, 130, 172, 280, 631, 937, 965, 1030
NlaIV GGNNCC 2 cut(s) 586, 967
NsiI ATGCAT 1 cut(s) 74
NspI RCATGY 1 cut(s) 11
PaeI GCATGC 1 cut(s) 11
PagI TCATGA 1 cut(s) 186
PctI GAATGC 1 cut(s) 696
PdmI GAANNNNTTC 2 cut(s) 322, 610
PfeI GAWTC 3 cut(s) 203, 339, 1081
PflMI CCANNNNNTGG 1 cut(s) 818
PkrI GCNGC 4 cut(s) 97, 561, 681, 1314
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
Ppu21I YACGTR 2 cut(s) 14, 237
Psp124BI GAGCTC 2 cut(s) 194, 675
PspN4I GGNNCC 2 cut(s) 586, 967
PsuI RGATCY 3 cut(s) 89, 172, 965
RsaI GTAC 4 cut(s) 16, 469, 730, 1063
RsaNI GTAC 4 cut(s) 15, 468, 729, 1062
SacI GAGCTC 2 cut(s) 194, 675
SaqAI TTAA 5 cut(s) 42, 548, 773, 1035, 1250
SatI GCNGC 4 cut(s) 96, 560, 680, 1313
Sau3AI GATC 8 cut(s) 89, 130, 172, 280, 631, 937, 965, 1030
SchI GAGTC 1 cut(s) 267
SduI GDGCHC 3 cut(s) 80, 194, 675
SfaNI GCATC 4 cut(s) 91, 171, 631, 1195
SfcI CTRYAG 1 cut(s) 312
SmlI CTYRAG 3 cut(s) 146, 168, 1070
SmoI CTYRAG 3 cut(s) 146, 168, 1070
SpeI ACTAGT 2 cut(s) 110, 1064
SphI GCATGC 1 cut(s) 11
SsiI CCGC 7 cut(s) 95, 219, 438, 559, 679, 809, 1312
SspI AATATT 2 cut(s) 754, 880
SspMI CTAG 6 cut(s) 111, 336, 347, 906, 1065, 1145
SstI GAGCTC 2 cut(s) 194, 675
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 2 cut(s) 589, 1061
TaiI ACGT 3 cut(s) 16, 239, 927
TatI WGTACW 2 cut(s) 467, 1061
TauI GCSGC 4 cut(s) 98, 562, 682, 1315
TfiI GAWTC 3 cut(s) 203, 339, 1081
Tru1I TTAA 5 cut(s) 42, 548, 773, 1035, 1250
Tru9I TTAA 5 cut(s) 42, 548, 773, 1035, 1250
TscAI CASTG 2 cut(s) 470, 586
TspDTI ATGAA 6 cut(s) 41, 49, 138, 216, 253, 353
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 2 cut(s) 470, 586
Van91I CCANNNNNTGG 1 cut(s) 818
XagI CCTNNNNNAGG 1 cut(s) 312
XapI RAATTY 7 cut(s) 372, 444, 845, 866, 1046, 1169, 1214
XbaI TCTAGA 1 cut(s) 905
XceI RCATGY 1 cut(s) 11
XmaJI CCTAGG 1 cut(s) 346
XmnI GAANNNNTTC 2 cut(s) 322, 610
XspI CTAG 6 cut(s) 111, 336, 347, 906, 1065, 1145
Zsp2I ATGCAT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.