MD04G1158300.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008883
Physical Location & Seq
Reverse (-)
24627033 .. 24628478
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1158300.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGAGCACTTGCCTTGAATCTACAGCACCTCGCAGTCTTACTCTGCTTGGAAATGAATCTGATCACTTGGCTCTGCTAGACTTCAAGAAAAAAATAACCGCAGATCCTTTCGATGTCATGAGCTCGTGGAATCATTCCATCCATTTCTGCAATTGGGTTGGGGTTTCTTGCCATCGTTCCACCAAAAGAGTCTTGATGTTGAACCTGATATCAAAAAAGTTGGTAGGCTCCATTCCACCTTCCGTTGGAAATCTTACTTATCTTATTGCAATCAATTTGAAAGACAACAATTTTCATGGGGGAATTCCTTCGGAAATGGGTCGTCTACAAAGCCTACAATATCTCAACCTTTCTCATAATTCCTTCCGTGAGAAAATTCCAACTAATTTGTCGCAATGCGCACAACTAAGAATGCTTAATTTGCAATACAATCATATTAAGGGGTCCATTCCGAACCAACTCAGTTCATTGTTGAATTTAGAACATCTATGGCTTTATGGTAACAATCTCACTGGAACCATCCCACCTTGGATAGGAAACTTTTCTTTGTTGAGCAGTCTTTATCTTGGCGAAAACAATTTTCAAGGAAGCATACCCAATGAGCTGGGCCTTATAACAGGCTTGGAGGAATTCGTAGTTGAGGGGAATAATCTATCTGGTATGGTTCCTTCCACAATCTATAATATTTCTTCAATATACACTTTTAGTGTTACTGAAAACCAGTTGCATGGAGAGCTACCACCAAATCTCGGCATTATGCTCCCTAACCTCGTAAAATTTTTCTGTGGTATGAACAAATTCACAGGAAATATTCCTGTATCTTTGTCAAATGCTTCTAGACTTCAAAGGTTTGATATTGCTGCAAATAAGCTCACTGGGTCAGTCCCTGGTGAAGATATCGGAAGCTTGAAGAGCTTAGTTTGGCTAAACTTTGAATCCAATCGACTGGGAAATGGAAAAAACGGTGATTTGAATTTTTTTAGTTTCTTGGCTAATTGCACTAGTTTGGAAACATTGAGTATTTTCCAAAATCATTTTGGAGGAGGAATCCCGGGTTCCCTTGCCAACCTTTCAACCCAACTAAAGTCCCTTACTCTTGGGGAAAATTTGATACATGGAAGCCTCCCTAATAGCATTGGAAATCTTATAAACTTGAACCGTCTAGGAATGGAGCGTAACTATTTGGATCATAGTGTCCCTAATGAAATTGGGAAGCTTCAAAAGTTAGTGGTACTTTTTTTGGGTGGCAACAAGTTTTCTGGGCCAATTCCGTCCTCCTTGGGTAACATCACTTCGTTAACAGAGCTCTACATGGAGGACAACGGGTTTTCTGGAAGTATACCTCCACAACAACAACAACAACAAAGCCTTTTCCCACTAAGTGGGGTCGGCTATATGAATCCTAGAACGCCATTGCGCTCGGTTTTGTGTCATGTCCTCCGTTAG

Protein Analysis

482

Amino Acids

52.8

Weight (kDa)

8.12

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 19 - 57 6.8e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 63 - 166 2.7e-08 Leucine-rich repeat region
LRR_8 PF13855 91 - 146 1e-07 Leucine rich repeat
LRR_4 PF12799 135 - 177 7.8e-08 Leucine Rich repeats (2 copies)
LRR_14 PF23598 359 - 438 1.3e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 614, 1148
Acc16I TGCGCA 1 cut(s) 400
AccB7I CCANNNNNTGG 1 cut(s) 1382
AccI GTMKAC 2 cut(s) 325, 1339
AciI CCGC 1 cut(s) 99
AclWI GGATC 2 cut(s) 98, 1194
AcsI RAATTY 8 cut(s) 303, 375, 475, 629, 776, 797, 973, 1105
AdeI CACNNNGTG 1 cut(s) 1382
AfaI GTAC 1 cut(s) 1233
AfiI CCNNNNNNNGG 4 cut(s) 245, 533, 749, 1382
AhlI ACTAGT 1 cut(s) 1001
AjnI CCWGG 1 cut(s) 886
AluBI AGCT 8 cut(s) 123, 604, 736, 871, 906, 915, 1216, 1306
AluI AGCT 8 cut(s) 123, 604, 736, 871, 906, 915, 1216, 1306
Alw21I GWGCWC 3 cut(s) 8, 125, 1308
AlwI GGATC 2 cut(s) 98, 1194
AlwNI CAGNNNCTG 1 cut(s) 887
Ama87I CYCGRG 1 cut(s) 1051
AoxI GGCC 2 cut(s) 607, 1262
ApeKI GCWGC 1 cut(s) 860
ApoI RAATTY 8 cut(s) 303, 375, 475, 629, 776, 797, 973, 1105
Asp700I GAANNNNTTC 3 cut(s) 307, 541, 797
AspLEI GCGC 2 cut(s) 401, 1419
AspS9I GGNCC 3 cut(s) 444, 607, 1262
AsuC2I CCSGG 2 cut(s) 1052, 1053
AsuHPI GGTGA 2 cut(s) 902, 977
AvaI CYCGRG 1 cut(s) 1051
AvaII GGWCC 1 cut(s) 444
BanII GRGCYC 2 cut(s) 125, 1308
BarI GAAGNNNNNNTAC 4 cut(s) 652, 684, 1276, 1308
BauI CACGAG 1 cut(s) 124
Bbv12I GWGCWC 3 cut(s) 8, 125, 1308
BbvI GCAGC 1 cut(s) 847
BccI CCATC 3 cut(s) 146, 180, 527
BciT130I CCWGG 1 cut(s) 888
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 2 cut(s) 1052, 1053
BcuI ACTAGT 1 cut(s) 1001
BfaI CTAG 5 cut(s) 77, 837, 1002, 1163, 1404
BfmI CTRYAG 1 cut(s) 21
BisI GCNGC 1 cut(s) 861
BlsI GCNGC 1 cut(s) 862
Bme1390I CCNGG 3 cut(s) 888, 1052, 1053
Bme18I GGWCC 1 cut(s) 444
BmeT110I CYCGRG 1 cut(s) 1051
BmgT120I GGNCC 3 cut(s) 444, 607, 1262
BmiI GGNNCC 5 cut(s) 229, 445, 517, 666, 1057
BmrFI CCNGG 3 cut(s) 888, 1052, 1053
BmrI ACTGGG 2 cut(s) 885, 956
BmuI ACTGGG 2 cut(s) 885, 956
BpuMI CCSGG 2 cut(s) 1052, 1053
BsaJI CCNNGG 4 cut(s) 527, 886, 1051, 1278
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 533, 749, 1382
Bse1I ACTGG 4 cut(s) 517, 721, 880, 951
Bse3DI GCAATG 2 cut(s) 401, 1412
BseBI CCWGG 1 cut(s) 888
BseDI CCNNGG 4 cut(s) 527, 886, 1051, 1278
BseGI GGATG 2 cut(s) 138, 519
BseLI CCNNNNNNNGG 4 cut(s) 245, 533, 749, 1382
BseMI GCAATG 2 cut(s) 401, 1412
BseMII CTCAG 1 cut(s) 475
BseNI ACTGG 4 cut(s) 517, 721, 880, 951
BseRI GAGGAG 1 cut(s) 1056
BseXI GCAGC 1 cut(s) 847
BseYI CCCAGC 1 cut(s) 604
BshFI GGCC 2 cut(s) 609, 1264
BsiHKAI GWGCWC 3 cut(s) 8, 125, 1308
BsiHKCI CYCGRG 1 cut(s) 1051
BsiSI CCGG 1 cut(s) 1052
BslFI GGGAC 3 cut(s) 869, 1072, 1181
BslI CCNNNNNNNGG 4 cut(s) 245, 533, 749, 1382
BsmFI GGGAC 3 cut(s) 869, 1072, 1181
BsmI GAATGC 1 cut(s) 417
BsnI GGCC 2 cut(s) 609, 1264
BsoBI CYCGRG 1 cut(s) 1051
Bsp1286I GDGCHC 3 cut(s) 8, 125, 1308
Bsp143I GATC 3 cut(s) 61, 103, 1186
BspACI CCGC 1 cut(s) 99
BspANI GGCC 2 cut(s) 609, 1264
BspCNI CTCAG 1 cut(s) 474
BspHI TCATGA 1 cut(s) 117
BspLI GGNNCC 5 cut(s) 229, 445, 517, 666, 1057
BspPI GGATC 2 cut(s) 98, 1194
BspQI GCTCTTC 1 cut(s) 905
BsrDI GCAATG 2 cut(s) 401, 1412
BsrI ACTGG 4 cut(s) 517, 721, 880, 951
BssECI CCNNGG 4 cut(s) 527, 886, 1051, 1278
BssMI GATC 3 cut(s) 61, 103, 1186
BssNAI GTATAC 1 cut(s) 1340
BssSI CACGAG 1 cut(s) 124
BssT1I CCWWGG 2 cut(s) 527, 1278
Bst1107I GTATAC 1 cut(s) 1340
Bst2BI CACGAG 1 cut(s) 124
Bst2UI CCWGG 1 cut(s) 888
Bst4CI ACNGT 2 cut(s) 965, 1160
Bst6I CTCTTC 1 cut(s) 905
BstDEI CTNAG 4 cut(s) 407, 461, 916, 1379
BstENI CCTNNNNNAGG 1 cut(s) 531
BstF5I GGATG 2 cut(s) 138, 519
BstHHI GCGC 2 cut(s) 401, 1419
BstKTI GATC 3 cut(s) 64, 106, 1189
BstMBI GATC 3 cut(s) 61, 103, 1186
BstMWI GCNNNNNNNGC 3 cut(s) 421, 733, 912
BstNI CCWGG 1 cut(s) 888
BstSCI CCNGG 3 cut(s) 886, 1050, 1051
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 847
BstX2I RGATCY 1 cut(s) 103
BstXI CCANNNNNNTGG 3 cut(s) 604, 728, 946
BstYI RGATCY 1 cut(s) 103
BstZ17I GTATAC 1 cut(s) 1340
BsuRI GGCC 2 cut(s) 609, 1264
BtsCI GGATG 2 cut(s) 138, 519
BtsIMutI CAGTG 2 cut(s) 510, 873
CaiI CAGNNNCTG 1 cut(s) 887
CciI TCATGA 1 cut(s) 117
CfoI GCGC 2 cut(s) 401, 1419
Cfr13I GGNCC 3 cut(s) 444, 607, 1262
Cfr9I CCCGGG 1 cut(s) 1051
Csp6I GTAC 1 cut(s) 1232
CviAII CATG 6 cut(s) 118, 296, 728, 1115, 1312, 1433
CviQI GTAC 1 cut(s) 1232
DdeI CTNAG 4 cut(s) 407, 461, 916, 1379
DpnI GATC 3 cut(s) 63, 105, 1188
DpnII GATC 3 cut(s) 61, 103, 1186
DraIII CACNNNGTG 1 cut(s) 1382
Eam1104I CTCTTC 1 cut(s) 905
EarI CTCTTC 1 cut(s) 905
Ecl136II GAGCTC 2 cut(s) 123, 1306
Eco130I CCWWGG 2 cut(s) 527, 1278
Eco24I GRGCYC 2 cut(s) 125, 1308
Eco32I GATATC 2 cut(s) 210, 898
Eco47I GGWCC 1 cut(s) 444
Eco53kI GAGCTC 2 cut(s) 123, 1306
Eco88I CYCGRG 1 cut(s) 1051
EcoICRI GAGCTC 2 cut(s) 123, 1306
EcoNI CCTNNNNNAGG 1 cut(s) 531
EcoRI GAATTC 2 cut(s) 303, 629
EcoRII CCWGG 1 cut(s) 886
EcoRV GATATC 2 cut(s) 210, 898
EcoT14I CCWWGG 2 cut(s) 527, 1278
EcoT38I GRGCYC 2 cut(s) 125, 1308
ErhI CCWWGG 2 cut(s) 527, 1278
FaeI CATG 6 cut(s) 121, 299, 731, 1118, 1315, 1436
FaqI GGGAC 3 cut(s) 869, 1072, 1181
FatI CATG 6 cut(s) 117, 295, 727, 1114, 1311, 1432
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 2 cut(s) 325, 1339
Fnu4HI GCNGC 1 cut(s) 861
FokI GGATG 2 cut(s) 125, 506
FriOI GRGCYC 2 cut(s) 125, 1308
Fsp4HI GCNGC 1 cut(s) 861
FspAI RTGCGCAY 1 cut(s) 400
FspBI CTAG 5 cut(s) 77, 837, 1002, 1163, 1404
FspI TGCGCA 1 cut(s) 400
GlaI GCGC 2 cut(s) 400, 1418
GluI GCNGC 1 cut(s) 861
GsaI CCCAGC 1 cut(s) 608
HaeIII GGCC 2 cut(s) 609, 1264
HapII CCGG 1 cut(s) 1052
HhaI GCGC 2 cut(s) 401, 1419
Hin1II CATG 6 cut(s) 121, 299, 731, 1118, 1315, 1436
Hin6I GCGC 2 cut(s) 399, 1417
HinP1I GCGC 2 cut(s) 399, 1417
HincII GTYRAC 1 cut(s) 1299
HindII GTYRAC 1 cut(s) 1299
HindIII AAGCTT 2 cut(s) 904, 1214
HinfI GANTC 7 cut(s) 17, 56, 130, 189, 935, 1047, 1399
HpaI GTTAAC 1 cut(s) 1299
HpaII CCGG 1 cut(s) 1052
HphI GGTGA 2 cut(s) 902, 977
Hpy166II GTNNAC 3 cut(s) 326, 1299, 1340
Hpy188I TCNGA 4 cut(s) 61, 313, 453, 902
Hpy188III TCNNGA 5 cut(s) 85, 118, 193, 837, 1332
Hpy8I GTNNAC 3 cut(s) 326, 1299, 1340
HpyAV CCTTC 4 cut(s) 249, 318, 373, 678
HpyCH4III ACNGT 2 cut(s) 965, 1160
HpyCH4V TGCA 6 cut(s) 150, 269, 424, 727, 863, 999
HpyF10VI GCNNNNNNNGC 3 cut(s) 421, 733, 912
HpyF3I CTNAG 4 cut(s) 407, 461, 916, 1379
Hsp92II CATG 6 cut(s) 121, 299, 731, 1118, 1315, 1436
HspAI GCGC 2 cut(s) 399, 1417
Ksp22I TGATCA 1 cut(s) 61
KspAI GTTAAC 1 cut(s) 1299
Kzo9I GATC 3 cut(s) 61, 103, 1186
LguI GCTCTTC 1 cut(s) 905
LmnI GCTCC 3 cut(s) 233, 765, 1171
Lsp1109I GCAGC 1 cut(s) 847
MaeI CTAG 5 cut(s) 77, 837, 1002, 1163, 1404
MaeIII GTNAC 4 cut(s) 500, 709, 1175, 1283
MalI GATC 3 cut(s) 63, 105, 1188
MboI GATC 3 cut(s) 61, 103, 1186
MboII GAAGA 3 cut(s) 681, 905, 922
MfeI CAATTG 1 cut(s) 151
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 3 cut(s) 8, 125, 1308
MlyI GAGTC 1 cut(s) 198
MmeI TCCRAC 2 cut(s) 226, 404
MroXI GAANNNNTTC 3 cut(s) 307, 541, 797
MseI TTAA 3 cut(s) 417, 438, 1298
MspI CCGG 1 cut(s) 1052
MspR9I CCNGG 3 cut(s) 888, 1052, 1053
MunI CAATTG 1 cut(s) 151
Mva1269I GAATGC 1 cut(s) 417
MvaI CCWGG 1 cut(s) 888
MwoI GCNNNNNNNGC 3 cut(s) 421, 733, 912
NciI CCSGG 2 cut(s) 1052, 1053
NdeII GATC 3 cut(s) 61, 103, 1186
NlaIII CATG 6 cut(s) 121, 299, 731, 1118, 1315, 1436
NlaIV GGNNCC 5 cut(s) 229, 445, 517, 666, 1057
NmeAIII GCCGAG 1 cut(s) 729
NsbI TGCGCA 1 cut(s) 400
PagI TCATGA 1 cut(s) 117
PciSI GCTCTTC 1 cut(s) 905
PctI GAATGC 1 cut(s) 417
PdmI GAANNNNTTC 3 cut(s) 307, 541, 797
PfeI GAWTC 6 cut(s) 17, 56, 130, 935, 1047, 1399
PflMI CCANNNNNTGG 1 cut(s) 1382
PkrI GCNGC 1 cut(s) 862
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
PsiI TTATAA 2 cut(s) 614, 1148
Psp124BI GAGCTC 2 cut(s) 125, 1308
Psp6I CCWGG 1 cut(s) 886
PspFI CCCAGC 1 cut(s) 604
PspGI CCWGG 1 cut(s) 886
PspN4I GGNNCC 5 cut(s) 229, 445, 517, 666, 1057
PspPI GGNCC 3 cut(s) 444, 607, 1262
PstNI CAGNNNCTG 1 cut(s) 887
PsuI RGATCY 1 cut(s) 103
RsaI GTAC 1 cut(s) 1233
RsaNI GTAC 1 cut(s) 1232
SacI GAGCTC 2 cut(s) 125, 1308
SapI GCTCTTC 1 cut(s) 905
SaqAI TTAA 3 cut(s) 417, 438, 1298
SatI GCNGC 1 cut(s) 861
Sau3AI GATC 3 cut(s) 61, 103, 1186
Sau96I GGNCC 3 cut(s) 444, 607, 1262
SchI GAGTC 1 cut(s) 198
ScrFI CCNGG 3 cut(s) 888, 1052, 1053
SduI GDGCHC 3 cut(s) 8, 125, 1308
SfcI CTRYAG 1 cut(s) 21
SinI GGWCC 1 cut(s) 444
SmaI CCCGGG 1 cut(s) 1053
SpeI ACTAGT 1 cut(s) 1001
SsiI CCGC 1 cut(s) 99
SspI AATATT 2 cut(s) 685, 811
SspMI CTAG 5 cut(s) 77, 837, 1002, 1163, 1404
SstI GAGCTC 2 cut(s) 125, 1308
StyD4I CCNGG 3 cut(s) 886, 1050, 1051
StyI CCWWGG 2 cut(s) 527, 1278
TaaI ACNGT 2 cut(s) 965, 1160
TaqI TCGA 2 cut(s) 111, 943
TfiI GAWTC 6 cut(s) 17, 56, 130, 935, 1047, 1399
Tru1I TTAA 3 cut(s) 417, 438, 1298
Tru9I TTAA 3 cut(s) 417, 438, 1298
TscAI CASTG 2 cut(s) 517, 880
TseI GCWGC 1 cut(s) 860
TspDTI ATGAA 6 cut(s) 69, 284, 456, 806, 1218, 1412
TspGWI ACGGA 4 cut(s) 232, 356, 1260, 1430
TspMI CCCGGG 1 cut(s) 1051
TspRI CASTG 2 cut(s) 517, 880
Van91I CCANNNNNTGG 1 cut(s) 1382
VpaK11BI GGWCC 1 cut(s) 444
XagI CCTNNNNNAGG 1 cut(s) 531
XapI RAATTY 8 cut(s) 303, 375, 475, 629, 776, 797, 973, 1105
XbaI TCTAGA 1 cut(s) 836
XcmI CCANNNNNNNNNTGG 1 cut(s) 1034
XmaI CCCGGG 1 cut(s) 1051
XmiI GTMKAC 2 cut(s) 325, 1339
XmnI GAANNNNTTC 3 cut(s) 307, 541, 797
XspI CTAG 5 cut(s) 77, 837, 1002, 1163, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.