MD12G1134900.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008883
Physical Location & Seq
Reverse (-)
21097473 .. 21097916
444 bp
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UTR
Exon/CDS
Intron
MD12G1134900.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGAGCATTCAAGTAGTACTCAAGTTAGGCTGTTTAAACTCTTTTATGGCTTCATTTTTTTGTGCATGAGTGCCGCAAGTCTGGAATCTGCGACACTCCCCAGCAGTAGCACTACCCTTGGAAATGAAGCTGATCGCATGGTGCTAGTCGACTTCAAGAAAAGAATCGTTGATGATCCTTTTCTTATCATGAGCTCATGGAATGATTCTATCCATTTCTGCAGTTGGGTAGGCGTTACATGTAACAATTCCACCAAAAGAGTCTTGACGTTGGAACTGCAGTCTCATAAATTGGTCGGCGCCATGCCACCTTATATTGGAAATCTTACTTATCTTACTGGAATCAATCTGAAAAGGAACAATTTTCATGGTGAACTTCCTCAAGAATTGGGTCGTCTACAAAGCCTGCAACACCTGTCAATTCCTTCAGCGGGAAGATGTTGA

Protein Analysis

148

Amino Acids

16.43

Weight (kDa)

8.42

Isoelectric Point (pI)

47.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 43 - 82 7.1e-10 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 299
AccI GTMKAC 2 cut(s) 150, 397
AciI CCGC 2 cut(s) 75, 431
AclWI GGATC 1 cut(s) 170
AcuI CTGAAG 1 cut(s) 411
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 1 cut(s) 19
AfiI CCNNNNNNNGG 2 cut(s) 317, 431
AflIII ACRYGT 1 cut(s) 239
AgsI TTSAA 2 cut(s) 12, 157
AluBI AGCT 2 cut(s) 131, 195
AluI AGCT 2 cut(s) 131, 195
Alw21I GWGCWC 1 cut(s) 197
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 1 cut(s) 170
AspLEI GCGC 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 383
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 197
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 146
BfmI CTRYAG 2 cut(s) 220, 278
BfoI RGCGCY 1 cut(s) 303
BisI GCNGC 1 cut(s) 75
BlsI GCNGC 1 cut(s) 76
BmcAI AGTACT 1 cut(s) 19
BmiI GGNNCC 1 cut(s) 301
BpuEI CTTGAG 2 cut(s) 6, 366
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 118
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 431
Bse1I ACTGG 1 cut(s) 343
BseDI CCNNGG 1 cut(s) 118
BseLI CCNNNNNNNGG 2 cut(s) 317, 431
BseNI ACTGG 1 cut(s) 343
BseYI CCCAGC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 197
BslI CCNNNNNNNGG 2 cut(s) 317, 431
BsmAI GTCTC 1 cut(s) 288
BsmI GAATGC 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 197
Bsp143I GATC 2 cut(s) 133, 175
BspACI CCGC 2 cut(s) 75, 431
BspHI TCATGA 1 cut(s) 189
BspLI GGNNCC 1 cut(s) 301
BspMAI CTGCAG 2 cut(s) 224, 282
BspPI GGATC 1 cut(s) 170
BspT107I GGYRCC 1 cut(s) 299
BsrI ACTGG 1 cut(s) 343
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 2 cut(s) 133, 175
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 1 cut(s) 118
BstACI GRCGYC 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 407
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 2 cut(s) 136, 178
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 2 cut(s) 133, 175
BstNSI RCATGY 1 cut(s) 243
BstSFI CTRYAG 2 cut(s) 220, 278
Cac8I GCNNGC 1 cut(s) 407
CciI TCATGA 1 cut(s) 189
CfoI GCGC 1 cut(s) 302
Csp6I GTAC 1 cut(s) 18
CviAII CATG 7 cut(s) 67, 139, 190, 198, 240, 304, 368
CviJI RGCY 5 cut(s) 31, 51, 131, 195, 405
CviKI_1 RGCY 5 cut(s) 31, 51, 131, 195, 405
CviQI GTAC 1 cut(s) 18
DinI GGCGCC 1 cut(s) 301
DpnI GATC 2 cut(s) 135, 177
DpnII GATC 2 cut(s) 133, 175
DraI TTTAAA 1 cut(s) 37
Ecl136II GAGCTC 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 118
Eco24I GRGCYC 1 cut(s) 197
Eco53kI GAGCTC 1 cut(s) 195
Eco57I CTGAAG 1 cut(s) 411
EcoICRI GAGCTC 1 cut(s) 195
EcoT14I CCWWGG 1 cut(s) 118
EcoT38I GRGCYC 1 cut(s) 197
EgeI GGCGCC 1 cut(s) 301
EheI GGCGCC 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 7 cut(s) 70, 142, 193, 201, 243, 307, 371
FatI CATG 7 cut(s) 66, 138, 189, 197, 239, 303, 367
FauI CCCGC 1 cut(s) 424
FblI GTMKAC 2 cut(s) 150, 397
Fnu4HI GCNGC 1 cut(s) 75
FriOI GRGCYC 1 cut(s) 197
Fsp4HI GCNGC 1 cut(s) 75
FspBI CTAG 1 cut(s) 146
GlaI GCGC 1 cut(s) 301
GluI GCNGC 1 cut(s) 75
GsaI CCCAGC 1 cut(s) 105
HaeII RGCGCY 1 cut(s) 303
HhaI GCGC 1 cut(s) 302
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 7 cut(s) 70, 142, 193, 201, 243, 307, 371
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 5 cut(s) 86, 165, 206, 261, 342
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 3 cut(s) 151, 374, 398
Hpy188I TCNGA 1 cut(s) 351
Hpy188III TCNNGA 5 cut(s) 83, 157, 190, 265, 383
Hpy8I GTNNAC 3 cut(s) 151, 374, 398
HpyAV CCTTC 1 cut(s) 435
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 4 cut(s) 66, 222, 280, 409
HpySE526I ACGT 1 cut(s) 269
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 7 cut(s) 70, 142, 193, 201, 243, 307, 371
HspAI GCGC 1 cut(s) 300
KasI GGCGCC 1 cut(s) 299
Kzo9I GATC 2 cut(s) 133, 175
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 5 cut(s) 68, 115, 324, 419, 428
MaeI CTAG 1 cut(s) 146
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 2 cut(s) 235, 242
MalI GATC 2 cut(s) 135, 177
MboI GATC 2 cut(s) 133, 175
MhlI GDGCHC 1 cut(s) 197
MluCI AATT 5 cut(s) 247, 290, 361, 386, 420
Mly113I GGCGCC 1 cut(s) 300
MlyI GAGTC 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 1 cut(s) 390
MseI TTAA 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 431
MssI GTTTAAAC 1 cut(s) 37
Mva1269I GAATGC 1 cut(s) 7
NarI GGCGCC 1 cut(s) 300
NdeII GATC 2 cut(s) 133, 175
NlaIII CATG 7 cut(s) 70, 142, 193, 201, 243, 307, 371
NlaIV GGNNCC 1 cut(s) 301
NspI RCATGY 1 cut(s) 243
PagI TCATGA 1 cut(s) 189
PciI ACATGT 1 cut(s) 239
PctI GAATGC 1 cut(s) 7
PfeI GAWTC 4 cut(s) 86, 165, 206, 342
PkrI GCNGC 1 cut(s) 76
PleI GAGTC 1 cut(s) 269
PluTI GGCGCC 1 cut(s) 303
PmeI GTTTAAAC 1 cut(s) 37
PpsI GAGTC 1 cut(s) 269
PscI ACATGT 1 cut(s) 239
Psp124BI GAGCTC 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 101
PspN4I GGNNCC 1 cut(s) 301
PstI CTGCAG 2 cut(s) 224, 282
RsaI GTAC 1 cut(s) 19
RsaNI GTAC 1 cut(s) 18
SacI GAGCTC 1 cut(s) 197
SalI GTCGAC 1 cut(s) 149
SaqAI TTAA 1 cut(s) 36
SatI GCNGC 1 cut(s) 75
Sau3AI GATC 2 cut(s) 133, 175
ScaI AGTACT 1 cut(s) 19
SchI GAGTC 1 cut(s) 270
SduI GDGCHC 1 cut(s) 197
SetI ASST 5 cut(s) 133, 197, 272, 313, 417
SfcI CTRYAG 2 cut(s) 220, 278
SfoI GGCGCC 1 cut(s) 301
SmlI CTYRAG 2 cut(s) 21, 381
SmoI CTYRAG 2 cut(s) 21, 381
Sse9I AATT 5 cut(s) 247, 290, 361, 386, 420
SsiI CCGC 2 cut(s) 75, 431
SspDI GGCGCC 1 cut(s) 299
SspMI CTAG 1 cut(s) 146
SstI GAGCTC 1 cut(s) 197
StyI CCWWGG 1 cut(s) 118
TaiI ACGT 1 cut(s) 272
TaqI TCGA 1 cut(s) 150
TasI AATT 5 cut(s) 247, 290, 361, 386, 420
TatI WGTACW 1 cut(s) 17
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 4 cut(s) 86, 165, 206, 342
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TspDTI ATGAA 3 cut(s) 43, 141, 356
XceI RCATGY 1 cut(s) 243
XmiI GTMKAC 2 cut(s) 150, 397
XspI CTAG 1 cut(s) 146
ZrmI AGTACT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.