MD03G1246800.v1.1

Putative adipose-regulatory protein (Seipin)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003222
Physical Location & Seq
Reverse (-)
33432722 .. 33433475
754 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1246800.v1.1.491

Sequence Viewer

Length: 270 bp
ATGAAGGTAATGGTCGAGTTCTTGTCAGCAGACGGCAGTGTCACTGCTAGCTCAAGCTATCCGCGCGGGCCAATTCACTTCACTGAAACATTTCTCAAAACCACTCCTCTCATTGCCGGTTTACAATCAGGAACCCAAGTTCTGAACATAAAAACGATTGAACACATTGAAGGGCTGCAGCCGACAGCGTACTTGAAAGTGGTACTAGAGCAAAGAGCAGAGTGTTCCAAAATGGTGCGGGCATACGTCAAATTTACGCTGCATCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Seipin

Protein Analysis

90

Amino Acids

9.89

Weight (kDa)

8.85

Isoelectric Point (pI)

52.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Seipin PF06775 3 - 86 1.2e-06 Putative adipose-regulatory protein (Seipin)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AccII CGCG 2 cut(s) 64, 66
AciI CCGC 3 cut(s) 62, 66, 238
AcsI RAATTY 1 cut(s) 251
AfaI GTAC 2 cut(s) 191, 204
AgsI TTSAA 3 cut(s) 161, 170, 196
AloI GAACNNNNNNTCC 2 cut(s) 123, 155
AluBI AGCT 2 cut(s) 51, 57
AluI AGCT 2 cut(s) 51, 57
AoxI GGCC 1 cut(s) 68
ApeKI GCWGC 3 cut(s) 175, 178, 259
ApoI RAATTY 1 cut(s) 251
Asp700I GAANNNNTTC 1 cut(s) 90
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 1 cut(s) 68
AsuNHI GCTAGC 1 cut(s) 47
BbvI GCAGC 3 cut(s) 162, 190, 246
BceAI ACGGC 1 cut(s) 49
BfaI CTAG 3 cut(s) 48, 206, 268
BfmI CTRYAG 1 cut(s) 176
BisI GCNGC 3 cut(s) 176, 179, 260
BlsI GCNGC 3 cut(s) 177, 180, 261
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 1 cut(s) 133
BmtI GCTAGC 1 cut(s) 51
BpuEI CTTGAG 1 cut(s) 37
Bse118I RCCGGY 1 cut(s) 116
Bse3DI GCAATG 1 cut(s) 111
BseMI GCAATG 1 cut(s) 111
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 3 cut(s) 162, 190, 246
Bsh1236I CGCG 2 cut(s) 64, 66
BshFI GGCC 1 cut(s) 70
BsiSI CCGG 1 cut(s) 117
BsnI GGCC 1 cut(s) 70
BspACI CCGC 3 cut(s) 62, 66, 238
BspANI GGCC 1 cut(s) 70
BspFNI CGCG 2 cut(s) 64, 66
BspLI GGNNCC 1 cut(s) 133
BspMAI CTGCAG 1 cut(s) 180
BspOI GCTAGC 1 cut(s) 51
BsrDI GCAATG 1 cut(s) 111
BsrFI RCCGGY 1 cut(s) 116
BssAI RCCGGY 1 cut(s) 116
BstC8I GCNNGC 3 cut(s) 49, 68, 240
BstFNI CGCG 2 cut(s) 64, 66
BstHHI GCGC 1 cut(s) 66
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstSFI CTRYAG 1 cut(s) 176
BstUI CGCG 2 cut(s) 64, 66
BstV1I GCAGC 3 cut(s) 162, 190, 246
BsuRI GGCC 1 cut(s) 70
BtsI GCAGTG 2 cut(s) 42, 43
BtsIMutI CAGTG 3 cut(s) 42, 43, 81
Cac8I GCNNGC 3 cut(s) 49, 68, 240
CfoI GCGC 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 1 cut(s) 68
Csp6I GTAC 2 cut(s) 190, 203
CviJI RGCY 5 cut(s) 51, 57, 70, 175, 181
CviKI_1 RGCY 5 cut(s) 51, 57, 70, 175, 181
CviQI GTAC 2 cut(s) 190, 203
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
FaiI YATR 2 cut(s) 149, 244
FauI CCCGC 2 cut(s) 59, 231
Fnu4HI GCNGC 3 cut(s) 176, 179, 260
Fsp4HI GCNGC 3 cut(s) 176, 179, 260
FspBI CTAG 3 cut(s) 48, 206, 268
GlaI GCGC 1 cut(s) 65
GluI GCNGC 3 cut(s) 176, 179, 260
HaeIII GGCC 1 cut(s) 70
HapII CCGG 1 cut(s) 117
HhaI GCGC 1 cut(s) 66
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HpaII CCGG 1 cut(s) 117
Hpy166II GTNNAC 1 cut(s) 122
Hpy188I TCNGA 1 cut(s) 144
Hpy188III TCNNGA 1 cut(s) 129
Hpy8I GTNNAC 1 cut(s) 122
HpyAV CCTTC 1 cut(s) 164
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 2 cut(s) 178, 262
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpySE526I ACGT 1 cut(s) 246
HspAI GCGC 1 cut(s) 64
LpnPI CCDG 2 cut(s) 114, 130
Lsp1109I GCAGC 3 cut(s) 162, 190, 246
MaeI CTAG 3 cut(s) 48, 206, 268
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 1 cut(s) 40
MluCI AATT 2 cut(s) 72, 251
MnlI CCTC 1 cut(s) 117
MroXI GAANNNNTTC 1 cut(s) 90
MspI CCGG 1 cut(s) 117
MvnI CGCG 2 cut(s) 64, 66
MwoI GCNNNNNNNGC 1 cut(s) 63
NheI GCTAGC 1 cut(s) 47
NlaIV GGNNCC 1 cut(s) 133
NmuCI GTSAC 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 90
PkrI GCNGC 3 cut(s) 177, 180, 261
PspN4I GGNNCC 1 cut(s) 133
PspPI GGNCC 1 cut(s) 68
PstI CTGCAG 1 cut(s) 180
RsaI GTAC 2 cut(s) 191, 204
RsaNI GTAC 2 cut(s) 190, 203
SatI GCNGC 3 cut(s) 176, 179, 260
Sau96I GGNCC 1 cut(s) 68
SetI ASST 4 cut(s) 9, 53, 59, 249
SfcI CTRYAG 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 2 cut(s) 72, 251
SsiI CCGC 3 cut(s) 62, 66, 238
SspMI CTAG 3 cut(s) 48, 206, 268
TaiI ACGT 1 cut(s) 249
TaqI TCGA 1 cut(s) 15
TasI AATT 2 cut(s) 72, 251
TscAI CASTG 3 cut(s) 43, 49, 88
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 3 cut(s) 175, 178, 259
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 43, 49, 88
XapI RAATTY 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 90
XspI CTAG 3 cut(s) 48, 206, 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.