Rh5CG232000

Putative adipose-regulatory protein (Seipin)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003222
Physical Location & Seq
Forward (+)
24080944 .. 24081659
716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG232000.1

Sequence Viewer

Length: 435 bp
ATGAAACACCTGGTTGAGAAGCCTATTAAGACCATAGAGACTCTCAACTTTGATTACACCAAGCCAAGTCTAGTAGCTTTGGTGCCACTCATGTCTTCTAATGTTGGTGTTATACCTTATGGTCACAAATTGAAGATGATTGTTTCGTTGACTGTGCCTGACTCGGAATACAATCACAGACGGTGTGTCTTTCAGGTAAGAGTAGAGTTCTTGTCAGCTAATGGTGATGTCACTGCTAGTTCGAGCCATCCATGCATGCTGCAATTCAAAAGCCGACCAATTCAATACATTCAGACCTTTCTGAGAAGTGCTCTTCTTATTGCTGGTTTACAATCAGAAACCCAAGTTCTGAACATCAAAATCAATGAACACGCTGAAGCCAACTACCCGCTTGAAGGTGATACTGGAACAAAGAGCAGAATTCCACCATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Seipin

Protein Analysis

144

Amino Acids

16.2

Weight (kDa)

8.93

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Seipin PF06775 2 - 127 2e-30 Putative adipose-regulatory protein (Seipin)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 82
AciI CCGC 1 cut(s) 389
AcsI RAATTY 1 cut(s) 420
AcuI CTGAAG 1 cut(s) 396
AfiI CCNNNNNNNGG 1 cut(s) 395
AgsI TTSAA 4 cut(s) 133, 268, 284, 395
AhdI GACNNNNNGTC 1 cut(s) 185
AjnI CCWGG 1 cut(s) 9
AleI CACNNNNGTG 1 cut(s) 430
AluBI AGCT 2 cut(s) 77, 218
AluI AGCT 2 cut(s) 77, 218
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 32
ApeKI GCWGC 1 cut(s) 259
ApoI RAATTY 1 cut(s) 420
AsuHPI GGTGA 2 cut(s) 236, 410
BanI GGYRCC 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 1 cut(s) 246
BccI CCATC 1 cut(s) 255
BciT130I CCWGG 1 cut(s) 11
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 2 cut(s) 71, 237
BisI GCNGC 1 cut(s) 260
BlsI GCNGC 1 cut(s) 261
Bme1390I CCNGG 1 cut(s) 11
BmeRI GACNNNNNGTC 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 1 cut(s) 11
BpiI GAAGAC 1 cut(s) 87
BplI GAGNNNNNCTC 2 cut(s) 295, 327
BsaJI CCNNGG 1 cut(s) 428
Bsc4I CCNNNNNNNGG 1 cut(s) 395
Bse1I ACTGG 1 cut(s) 409
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 428
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 395
BseMII CTCAG 1 cut(s) 293
BseNI ACTGG 1 cut(s) 409
BseXI GCAGC 1 cut(s) 246
BshNI GGYRCC 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 313
BslI CCNNNNNNNGG 1 cut(s) 395
BsmAI GTCTC 1 cut(s) 32
Bsp1286I GDGCHC 1 cut(s) 313
Bsp19I CCATGG 1 cut(s) 428
BspACI CCGC 1 cut(s) 389
BspCNI CTCAG 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 84
BspQI GCTCTTC 1 cut(s) 318
BspT107I GGYRCC 1 cut(s) 82
BsrI ACTGG 1 cut(s) 409
BssECI CCNNGG 1 cut(s) 428
BssT1I CCWWGG 1 cut(s) 428
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 154, 183
Bst6I CTCTTC 1 cut(s) 318
BstC8I GCNNGC 1 cut(s) 257
BstDEI CTNAG 1 cut(s) 302
BstDSI CCRYGG 1 cut(s) 428
BstF5I GGATG 1 cut(s) 247
BstMAI GTCTC 1 cut(s) 32
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 9
BstV1I GCAGC 1 cut(s) 246
BstV2I GAAGAC 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 428
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 1 cut(s) 231
Cac8I GCNNGC 1 cut(s) 257
CsiI ACCWGGT 1 cut(s) 9
CviAII CATG 4 cut(s) 91, 252, 256, 429
CviJI RGCY 7 cut(s) 22, 64, 77, 218, 246, 273, 380
CviKI_1 RGCY 7 cut(s) 22, 64, 77, 218, 246, 273, 380
DdeI CTNAG 1 cut(s) 302
DriI GACNNNNNGTC 1 cut(s) 185
Eam1104I CTCTTC 1 cut(s) 318
Eam1105I GACNNNNNGTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 428
Eco57I CTGAAG 1 cut(s) 396
EcoRI GAATTC 1 cut(s) 420
EcoRII CCWGG 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 428
EcoT22I ATGCAT 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 428
FaeI CATG 4 cut(s) 94, 255, 259, 432
FaiI YATR 7 cut(s) 35, 92, 113, 120, 253, 257, 430
FatI CATG 4 cut(s) 90, 251, 255, 428
FauI CCCGC 1 cut(s) 396
Fnu4HI GCNGC 1 cut(s) 260
FokI GGATG 1 cut(s) 234
Fsp4HI GCNGC 1 cut(s) 260
FspBI CTAG 2 cut(s) 71, 237
GluI GCNGC 1 cut(s) 260
Hin1II CATG 4 cut(s) 94, 255, 259, 432
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HinfI GANTC 2 cut(s) 40, 161
HphI GGTGA 2 cut(s) 236, 410
Hpy166II GTNNAC 2 cut(s) 150, 329
Hpy188I TCNGA 5 cut(s) 166, 294, 303, 337, 351
Hpy8I GTNNAC 2 cut(s) 150, 329
HpyAV CCTTC 1 cut(s) 389
HpyCH4III ACNGT 2 cut(s) 154, 183
HpyCH4V TGCA 2 cut(s) 255, 262
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
HpyF3I CTNAG 1 cut(s) 302
Hsp92II CATG 4 cut(s) 94, 255, 259, 432
LguI GCTCTTC 1 cut(s) 318
LpnPI CCDG 5 cut(s) 23, 171, 179, 309, 390
Lsp1109I GCAGC 1 cut(s) 246
MabI ACCWGGT 1 cut(s) 9
MaeI CTAG 2 cut(s) 71, 237
MaeIII GTNAC 2 cut(s) 122, 229
MboII GAAGA 3 cut(s) 87, 145, 305
MhlI GDGCHC 1 cut(s) 313
MluCI AATT 4 cut(s) 128, 263, 279, 420
MlyI GAGTC 2 cut(s) 34, 155
Mph1103I ATGCAT 1 cut(s) 257
MseI TTAA 1 cut(s) 27
MslI CAYNNNNRTG 1 cut(s) 430
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 252
NcoI CCATGG 1 cut(s) 428
NlaIII CATG 4 cut(s) 94, 255, 259, 432
NlaIV GGNNCC 1 cut(s) 84
NmuCI GTSAC 2 cut(s) 122, 229
NsiI ATGCAT 1 cut(s) 257
NspI RCATGY 1 cut(s) 259
OliI CACNNNNGTG 1 cut(s) 430
PaeI GCATGC 1 cut(s) 259
PciSI GCTCTTC 1 cut(s) 318
PkrI GCNGC 1 cut(s) 261
PleI GAGTC 2 cut(s) 34, 155
PpsI GAGTC 2 cut(s) 34, 155
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 84
RseI CAYNNNNRTG 1 cut(s) 430
SapI GCTCTTC 1 cut(s) 318
SaqAI TTAA 1 cut(s) 27
SatI GCNGC 1 cut(s) 260
SchI GAGTC 2 cut(s) 34, 155
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 313
SetI ASST 7 cut(s) 12, 79, 118, 198, 220, 299, 400
SexAI ACCWGGT 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 430
SphI GCATGC 1 cut(s) 259
Sse9I AATT 4 cut(s) 128, 263, 279, 420
SsiI CCGC 1 cut(s) 389
SspMI CTAG 2 cut(s) 71, 237
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 1 cut(s) 428
TaaI ACNGT 2 cut(s) 154, 183
TaqI TCGA 1 cut(s) 242
TasI AATT 4 cut(s) 128, 263, 279, 420
Tru1I TTAA 1 cut(s) 27
Tru9I TTAA 1 cut(s) 27
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 2 cut(s) 122, 229
TseI GCWGC 1 cut(s) 259
Tsp45I GTSAC 2 cut(s) 122, 229
TspDTI ATGAA 2 cut(s) 17, 381
TspRI CASTG 1 cut(s) 238
XapI RAATTY 1 cut(s) 420
XceI RCATGY 1 cut(s) 259
XspI CTAG 2 cut(s) 71, 237
Zsp2I ATGCAT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.