MD11G1267400.v1.1

Putative adipose-regulatory protein (Seipin)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003222
Physical Location & Seq
Reverse (-)
38247574 .. 38248690
1117 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1267400.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGGTACATTCTCCATTACGAAGCTACAATCCATTTCTTTTTGAAAACTTTGGCATTCGTTGTGGCATTACTGATAAAATCATAGGTTTTCAACTTAGTCTAGTGGTCGCATTTTTCACGCTTCCAATTCGATTATCCTATTTCTCGTTCATGCTCTTCTTGTTTCCATTTCGAACTCTCAAAAATGTACCAAGAATGTGCAGTGTCATATCTAAAAATGTTACTTCAGCGGTATCCTGCCGCGTGCAAGCTCAAAAGTCAGTGGCAGTGAATGTAGGGTTTGCTTTCTTCAGGTCACTCTATGTGCGTTCCATGCTTCTTGGAATTCTGGCATTCGCGTTTGTACTAAGTGGATTTATTATGAGACACTTGGTTGAGAATCCAATTCAGACAACAGAAGCCCTCAACTTTGATTATACCAAATCATGTCCAGTTGCTTTTGTACCACTCATGTCATCTTCTCGTGTCAGTAGCGACCGTGCGATACCTTATAACCACAAATTGCAGCTCGCTGTTTCGTTAACTGCACCTGAATCCGAATATAATCACAAGCTGGGTGTAAGGGTCGAGTTCTTGTCAGCAAACGGCAGTGTCAAAGCTAGCTCAAGCCATCCGTGCATGCTGCGATACAAAAGCGAGCCAATTCACTTCATCGAAACATTTCTCAAAACCGCTCCTCTCATTACCGGTTTACAGTCAGAAACGCAAGTTCTGAACATAAAAATGAGCGGACACATTGAAGGACTCGAGCCGACTGCCTACTTGAAGGTGATACTAGAGCAAAGAGCCGAGTTCCAAAATGGTGCCGGCTTACCTCAAATTTACGCTGCATCACTAGTGCTCGAATCTGAGCTTCCTCGGTCAAAAAGGTTTGTTAGGAAGTGGAGGAGAACAATATTTGTGTGGACAAGTCTTGTTTCATTTTTGGCAGAGTTGATGTTCATCCTAGCATTTTTCAGACCTATTATTGTCCCAAGAGGAAGACCGAAAATATGTTACAGTACTAAGAAATTTGATCACTCAAACATTATTTTCTGGCAGAAAAGCAGTTATGTGCTTCCAAGGAACACTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Seipin

Protein Analysis

359

Amino Acids

40.73

Weight (kDa)

10.02

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Seipin PF06775 108 - 307 1.5e-47 Putative adipose-regulatory protein (Seipin)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
AccB1I GGYRCC 1 cut(s) 803
AccBSI CCGCTC 2 cut(s) 674, 729
AccII CGCG 2 cut(s) 243, 338
AciI CCGC 4 cut(s) 230, 241, 672, 729
AcsI RAATTY 3 cut(s) 324, 819, 1010
AcuI CTGAAG 2 cut(s) 210, 274
AfaI GTAC 5 cut(s) 6, 189, 345, 444, 1003
AgeI ACCGGT 1 cut(s) 686
AgsI TTSAA 4 cut(s) 44, 92, 740, 766
AhlI ACTAGT 1 cut(s) 835
AluBI AGCT 7 cut(s) 24, 251, 508, 553, 599, 603, 853
AluI AGCT 7 cut(s) 24, 251, 508, 553, 599, 603, 853
Alw21I GWGCWC 1 cut(s) 843
Alw26I GTCTC 1 cut(s) 358
Ama87I CYCGRG 1 cut(s) 746
ApeKI GCWGC 3 cut(s) 505, 622, 827
ApoI RAATTY 3 cut(s) 324, 819, 1010
AsiGI ACCGGT 1 cut(s) 686
Asp700I GAANNNNTTC 1 cut(s) 660
AsuHPI GGTGA 1 cut(s) 781
AsuII TTCGAA 1 cut(s) 172
AsuNHI GCTAGC 1 cut(s) 599
AvaI CYCGRG 1 cut(s) 746
BanI GGYRCC 1 cut(s) 803
BauI CACGAG 1 cut(s) 462
BbsI GAAGAC 1 cut(s) 988
Bbv12I GWGCWC 1 cut(s) 843
BbvI GCAGC 3 cut(s) 517, 609, 814
BccI CCATC 1 cut(s) 618
BceAI ACGGC 1 cut(s) 601
BcgI CGANNNNNNTGC 2 cut(s) 737, 771
BciVI GTATCC 1 cut(s) 244
BclI TGATCA 1 cut(s) 1015
BcoDI GTCTC 1 cut(s) 358
BcuI ACTAGT 1 cut(s) 835
BfaI CTAG 5 cut(s) 101, 600, 776, 836, 947
BfuI GTATCC 1 cut(s) 244
BisI GCNGC 4 cut(s) 241, 506, 623, 828
BlsI GCNGC 4 cut(s) 242, 507, 624, 829
BmcAI AGTACT 1 cut(s) 1003
BmeT110I CYCGRG 1 cut(s) 746
BmiI GGNNCC 1 cut(s) 805
BmsI GCATC 1 cut(s) 839
BmtI GCTAGC 1 cut(s) 603
BpiI GAAGAC 1 cut(s) 988
Bpu14I TTCGAA 1 cut(s) 172
BpuEI CTTGAG 1 cut(s) 589
BsaBI GATNNNNATC 1 cut(s) 941
BsaJI CCNNGG 2 cut(s) 857, 1061
BsaWI WCCGGW 1 cut(s) 686
Bse118I RCCGGY 2 cut(s) 686, 806
Bse1I ACTGG 1 cut(s) 431
Bse8I GATNNNNATC 1 cut(s) 941
BseDI CCNNGG 2 cut(s) 857, 1061
BseGI GGATG 2 cut(s) 610, 942
BseJI GATNNNNATC 1 cut(s) 941
BseMII CTCAG 1 cut(s) 840
BseNI ACTGG 1 cut(s) 431
BseRI GAGGAG 2 cut(s) 666, 901
BseXI GCAGC 3 cut(s) 517, 609, 814
BseYI CCCAGC 1 cut(s) 553
BsgI GTGCAG 2 cut(s) 220, 510
Bsh1236I CGCG 2 cut(s) 243, 338
Bsh1285I CGRYCG 1 cut(s) 478
BshNI GGYRCC 1 cut(s) 803
BshTI ACCGGT 1 cut(s) 686
BsiEI CGRYCG 1 cut(s) 478
BsiHKAI GWGCWC 1 cut(s) 843
BsiHKCI CYCGRG 1 cut(s) 746
BsiSI CCGG 2 cut(s) 687, 807
BslFI GGGAC 1 cut(s) 956
BsmAI GTCTC 1 cut(s) 358
BsmFI GGGAC 1 cut(s) 956
BsmI GAATGC 2 cut(s) 54, 332
BsoBI CYCGRG 1 cut(s) 746
Bsp119I TTCGAA 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 843
Bsp143I GATC 1 cut(s) 1015
BspACI CCGC 4 cut(s) 230, 241, 672, 729
BspCNI CTCAG 1 cut(s) 841
BspFNI CGCG 2 cut(s) 243, 338
BspLI GGNNCC 1 cut(s) 805
BspOI GCTAGC 1 cut(s) 603
BspQI GCTCTTC 1 cut(s) 161
BspT104I TTCGAA 1 cut(s) 172
BspT107I GGYRCC 1 cut(s) 803
BsrBI CCGCTC 2 cut(s) 674, 729
BsrFI RCCGGY 2 cut(s) 686, 806
BsrI ACTGG 1 cut(s) 431
BssAI RCCGGY 2 cut(s) 686, 806
BssECI CCNNGG 2 cut(s) 857, 1061
BssMI GATC 1 cut(s) 1015
BssSI CACGAG 1 cut(s) 462
BssT1I CCWWGG 1 cut(s) 1061
Bst2BI CACGAG 1 cut(s) 462
Bst4CI ACNGT 3 cut(s) 479, 696, 1001
Bst6I CTCTTC 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 172
BstC8I GCNNGC 7 cut(s) 245, 249, 510, 601, 620, 638, 808
BstDEI CTNAG 4 cut(s) 95, 347, 849, 1005
BstF5I GGATG 2 cut(s) 610, 942
BstFNI CGCG 2 cut(s) 243, 338
BstKTI GATC 1 cut(s) 1018
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 1 cut(s) 1015
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 2 cut(s) 313, 615
BstNSI RCATGY 1 cut(s) 622
BstUI CGCG 2 cut(s) 243, 338
BstV1I GCAGC 3 cut(s) 517, 609, 814
BstV2I GAAGAC 1 cut(s) 988
BsuI GTATCC 1 cut(s) 244
BtsCI GGATG 2 cut(s) 610, 942
BtsI GCAGTG 3 cut(s) 208, 273, 595
BtsIMutI CAGTG 4 cut(s) 208, 267, 273, 595
Cac8I GCNNGC 7 cut(s) 245, 249, 510, 601, 620, 638, 808
Cfr10I RCCGGY 2 cut(s) 686, 806
Csp6I GTAC 5 cut(s) 5, 188, 344, 443, 1002
CspAI ACCGGT 1 cut(s) 686
CviAII CATG 5 cut(s) 151, 313, 426, 451, 619
CviQI GTAC 5 cut(s) 5, 188, 344, 443, 1002
DdeI CTNAG 4 cut(s) 95, 347, 849, 1005
DpnI GATC 1 cut(s) 1017
DpnII GATC 1 cut(s) 1015
Eam1104I CTCTTC 1 cut(s) 161
EarI CTCTTC 1 cut(s) 161
Eco130I CCWWGG 1 cut(s) 1061
Eco57I CTGAAG 2 cut(s) 210, 274
Eco88I CYCGRG 1 cut(s) 746
EcoRI GAATTC 1 cut(s) 324
EcoT14I CCWWGG 1 cut(s) 1061
ErhI CCWWGG 1 cut(s) 1061
FaeI CATG 5 cut(s) 154, 316, 429, 454, 622
FaqI GGGAC 1 cut(s) 956
FatI CATG 5 cut(s) 150, 312, 425, 450, 618
FbaI TGATCA 1 cut(s) 1015
Fnu4HI GCNGC 4 cut(s) 241, 506, 623, 828
FokI GGATG 2 cut(s) 597, 929
Fsp4HI GCNGC 4 cut(s) 241, 506, 623, 828
FspBI CTAG 5 cut(s) 101, 600, 776, 836, 947
GluI GCNGC 4 cut(s) 241, 506, 623, 828
GsaI CCCAGC 1 cut(s) 557
HapII CCGG 2 cut(s) 687, 807
Hin1II CATG 5 cut(s) 154, 316, 429, 454, 622
HincII GTYRAC 1 cut(s) 522
HindII GTYRAC 1 cut(s) 522
HinfI GANTC 4 cut(s) 379, 533, 744, 845
HpaI GTTAAC 1 cut(s) 522
HpaII CCGG 2 cut(s) 687, 807
HphI GGTGA 1 cut(s) 781
Hpy166II GTNNAC 3 cut(s) 522, 692, 906
Hpy188I TCNGA 6 cut(s) 390, 538, 700, 714, 850, 959
Hpy8I GTNNAC 3 cut(s) 522, 692, 906
HpyAV CCTTC 2 cut(s) 734, 760
HpyCH4III ACNGT 3 cut(s) 479, 696, 1001
HpyCH4V TGCA 6 cut(s) 201, 247, 505, 527, 618, 830
HpyF10VI GCNNNNNNNGC 2 cut(s) 313, 615
HpyF3I CTNAG 4 cut(s) 95, 347, 849, 1005
Hsp92II CATG 5 cut(s) 154, 316, 429, 454, 622
KroI GCCGGC 1 cut(s) 806
KroNI GCCGGC 1 cut(s) 808
Ksp22I TGATCA 1 cut(s) 1015
KspAI GTTAAC 1 cut(s) 522
Kzo9I GATC 1 cut(s) 1015
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 1 cut(s) 679
LpnPI CCDG 9 cut(s) 250, 277, 314, 444, 539, 543, 700, 820, 1021
Lsp1109I GCAGC 3 cut(s) 517, 609, 814
LweI GCATC 1 cut(s) 839
MaeI CTAG 5 cut(s) 101, 600, 776, 836, 947
MaeIII GTNAC 3 cut(s) 220, 294, 995
MalI GATC 1 cut(s) 1017
MbiI CCGCTC 2 cut(s) 674, 729
MboI GATC 1 cut(s) 1015
MboII GAAGA 4 cut(s) 148, 280, 450, 993
MhlI GDGCHC 1 cut(s) 843
MluCI AATT 7 cut(s) 126, 324, 384, 500, 642, 819, 1010
MlyI GAGTC 1 cut(s) 738
MnlI CCTC 6 cut(s) 413, 687, 825, 867, 879, 971
MroNI GCCGGC 1 cut(s) 806
MroXI GAANNNNTTC 1 cut(s) 660
MseI TTAA 1 cut(s) 521
MslI CAYNNNNRTG 1 cut(s) 722
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 2 cut(s) 687, 807
Mva1269I GAATGC 2 cut(s) 54, 332
MvnI CGCG 2 cut(s) 243, 338
MwoI GCNNNNNNNGC 2 cut(s) 313, 615
NaeI GCCGGC 1 cut(s) 808
NdeII GATC 1 cut(s) 1015
NgoMIV GCCGGC 1 cut(s) 806
NheI GCTAGC 1 cut(s) 599
NlaIII CATG 5 cut(s) 154, 316, 429, 454, 622
NlaIV GGNNCC 1 cut(s) 805
NmeAIII GCCGAG 1 cut(s) 814
NmuCI GTSAC 1 cut(s) 294
NspI RCATGY 1 cut(s) 622
NspV TTCGAA 1 cut(s) 172
PaeI GCATGC 1 cut(s) 622
PaeR7I CTCGAG 1 cut(s) 746
PciSI GCTCTTC 1 cut(s) 161
PctI GAATGC 2 cut(s) 54, 332
PdiI GCCGGC 1 cut(s) 808
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 3 cut(s) 379, 533, 845
PinAI ACCGGT 1 cut(s) 686
PkrI GCNGC 4 cut(s) 242, 507, 624, 829
PleI GAGTC 1 cut(s) 738
PpsI GAGTC 1 cut(s) 738
PsiI TTATAA 1 cut(s) 492
PspFI CCCAGC 1 cut(s) 553
PspN4I GGNNCC 1 cut(s) 805
PspXI VCTCGAGB 1 cut(s) 746
RsaI GTAC 5 cut(s) 6, 189, 345, 444, 1003
RsaNI GTAC 5 cut(s) 5, 188, 344, 443, 1002
RseI CAYNNNNRTG 1 cut(s) 722
SapI GCTCTTC 1 cut(s) 161
SaqAI TTAA 1 cut(s) 521
SatI GCNGC 4 cut(s) 241, 506, 623, 828
Sau3AI GATC 1 cut(s) 1015
ScaI AGTACT 1 cut(s) 1003
SchI GAGTC 1 cut(s) 738
SduI GDGCHC 1 cut(s) 843
SfaNI GCATC 1 cut(s) 839
Sfr274I CTCGAG 1 cut(s) 746
SfuI TTCGAA 1 cut(s) 172
SlaI CTCGAG 1 cut(s) 746
SmiMI CAYNNNNRTG 1 cut(s) 722
SmlI CTYRAG 2 cut(s) 604, 746
SmoI CTYRAG 2 cut(s) 604, 746
SpeI ACTAGT 1 cut(s) 835
SphI GCATGC 1 cut(s) 622
Sse9I AATT 7 cut(s) 126, 324, 384, 500, 642, 819, 1010
SsiI CCGC 4 cut(s) 230, 241, 672, 729
SspI AATATT 1 cut(s) 897
SspMI CTAG 5 cut(s) 101, 600, 776, 836, 947
StyI CCWWGG 1 cut(s) 1061
TaaI ACNGT 3 cut(s) 479, 696, 1001
TaqI TCGA 6 cut(s) 130, 172, 567, 654, 747, 843
TaqII GACCGA 2 cut(s) 849, 1000
TasI AATT 7 cut(s) 126, 324, 384, 500, 642, 819, 1010
TatI WGTACW 2 cut(s) 343, 1001
TauI GCSGC 1 cut(s) 243
TfiI GAWTC 3 cut(s) 379, 533, 845
Tru1I TTAA 1 cut(s) 521
Tru9I TTAA 1 cut(s) 521
TscAI CASTG 4 cut(s) 208, 267, 273, 595
TseFI GTSAC 1 cut(s) 294
TseI GCWGC 3 cut(s) 505, 622, 827
Tsp45I GTSAC 1 cut(s) 294
TspDTI ATGAA 4 cut(s) 139, 640, 909, 931
TspGWI ACGGA 1 cut(s) 603
TspRI CASTG 4 cut(s) 208, 267, 273, 595
XapI RAATTY 3 cut(s) 324, 819, 1010
XceI RCATGY 1 cut(s) 622
XhoI CTCGAG 1 cut(s) 746
XmnI GAANNNNTTC 1 cut(s) 660
XspI CTAG 5 cut(s) 101, 600, 776, 836, 947
ZrmI AGTACT 1 cut(s) 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.