Rw5G019060

Putative adipose-regulatory protein (Seipin)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003222
Physical Location & Seq
Forward (+)
24834106 .. 24849708
15603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G019060.1

Sequence Viewer

Length: 867 bp
ATGGTATTGCTGATAAAAACTATAGGATTTCAAGTTAGCCTAGTGGTCAAATTTTACACTTTCCCAATTCGGTTATTCTATTTTTCCTTCATGCTCTTGCTATGTCCATTTCAAACTCTGATACACATTAGAGGGTACTTGATGACAATGCTGTTGAGATTGTGGACTGTATGTGGGGGATTTAAAACCCAAAAGTCATTTGCAGTGAAAATAGGATTCTCCTTGTTCTGGTCAATCCATGTGTGTTGCATGCTTTTGGGGATTCTGGCATCTGGATTTGTGCTAAGTGGGTTTGTGATGAAACACCTGGTTGAGAAGCCTATTAAGACCATAAAGACCCCCAACTTTGATTGCACTAAGCCAAGTTCAGTAGCTTTGGTGCCACTCATGTCTTCTAATGTTGGTGTTATACCTTATGGTCACAAATTGAAGATGATTGTTTCGTTGACTGTGCCCGACTCGGAATACAATTGTTTGGCTCCAAAACCAGTTGGCGTGCAAACTCTAATGGTGATGTCACTGCTAGTTCGAGCCATGCATCCATGCATGCTGCAATTCAAAAGCCGGCCAATTCAATACATTCAGACCTTTCTGAGAAGTGCTCCTCTTATTGCTGGTTTACAATCAGAAACCCAAGCTCTGAACATCAAAATCAATGAACACGCTGAAGGACTTGAGCCAACTACCCGCTTGAAGGTGATACTGGAACAAAGAGCAGAATTCCACCATGGTGAAGGCATACCTCAAACTTACGCTGGGTCATTACTCCTTGAATCTGAGCTTCCCCAATTGAAAAGATTTGTGTGGTGTTGGAGGAGAACAATATTCACCTATATATGTTTGTCCCAAGAGGGAGACCAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Seipin

Protein Analysis

288

Amino Acids

32.7

Weight (kDa)

9.49

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Seipin PF06775 88 - 281 3.3e-29 Putative adipose-regulatory protein (Seipin)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 379
AciI CCGC 1 cut(s) 688
AcoI YGGCCR 1 cut(s) 566
AcsI RAATTY 2 cut(s) 50, 719
AcuI CTGAAG 1 cut(s) 687
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 2 cut(s) 228, 694
AgsI TTSAA 8 cut(s) 32, 113, 430, 559, 575, 694, 773, 793
AjnI CCWGG 1 cut(s) 306
AleI CACNNNNGTG 1 cut(s) 729
AluBI AGCT 3 cut(s) 374, 638, 781
AluI AGCT 3 cut(s) 374, 638, 781
Alw21I GWGCWC 1 cut(s) 604
Alw26I GTCTC 1 cut(s) 849
AoxI GGCC 1 cut(s) 566
ApeKI GCWGC 1 cut(s) 550
ApoI RAATTY 2 cut(s) 50, 719
AsuHPI GGTGA 4 cut(s) 523, 709, 743, 820
BaeGI GKGCMC 1 cut(s) 456
BanI GGYRCC 1 cut(s) 379
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 604
BbvI GCAGC 1 cut(s) 537
BciT130I CCWGG 1 cut(s) 308
BcoDI GTCTC 1 cut(s) 849
BfaI CTAG 2 cut(s) 41, 524
BfmI CTRYAG 1 cut(s) 21
BisI GCNGC 1 cut(s) 551
BlsI GCNGC 1 cut(s) 552
Bme1390I CCNGG 1 cut(s) 308
BmiI GGNNCC 2 cut(s) 381, 480
BmrFI CCNGG 1 cut(s) 308
BmsI GCATC 2 cut(s) 278, 547
BpiI GAAGAC 1 cut(s) 384
BplI GAGNNNNNCTC 2 cut(s) 586, 618
BpuEI CTTGAG 1 cut(s) 695
BsaI GGTCTC 1 cut(s) 849
BsaJI CCNNGG 1 cut(s) 727
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 694
Bse118I RCCGGY 1 cut(s) 564
Bse1I ACTGG 2 cut(s) 488, 708
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 1 cut(s) 727
BseGI GGATG 1 cut(s) 538
BseLI CCNNNNNNNGG 2 cut(s) 228, 694
BseMII CTCAG 2 cut(s) 584, 768
BseNI ACTGG 2 cut(s) 488, 708
BseRI GAGGAG 2 cut(s) 594, 829
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 1 cut(s) 537
BseYI CCCAGC 1 cut(s) 755
BshFI GGCC 1 cut(s) 568
BshNI GGYRCC 1 cut(s) 379
BsiHKAI GWGCWC 1 cut(s) 604
BsiSI CCGG 1 cut(s) 565
BslFI GGGAC 1 cut(s) 829
BslI CCNNNNNNNGG 2 cut(s) 228, 694
BsmAI GTCTC 1 cut(s) 849
BsmFI GGGAC 1 cut(s) 829
BsnI GGCC 1 cut(s) 568
Bso31I GGTCTC 1 cut(s) 849
Bsp1286I GDGCHC 2 cut(s) 456, 604
Bsp19I CCATGG 1 cut(s) 727
BspACI CCGC 1 cut(s) 688
BspANI GGCC 1 cut(s) 568
BspCNI CTCAG 2 cut(s) 585, 769
BspLI GGNNCC 2 cut(s) 381, 480
BspT107I GGYRCC 1 cut(s) 379
BspTNI GGTCTC 1 cut(s) 849
BsrFI RCCGGY 1 cut(s) 564
BsrI ACTGG 2 cut(s) 488, 708
BssAI RCCGGY 1 cut(s) 564
BssECI CCNNGG 1 cut(s) 727
BssT1I CCWWGG 1 cut(s) 727
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 2 cut(s) 169, 451
BstC8I GCNNGC 4 cut(s) 251, 497, 548, 566
BstDEI CTNAG 4 cut(s) 284, 357, 593, 777
BstDSI CCRYGG 1 cut(s) 727
BstF5I GGATG 1 cut(s) 538
BstMAI GTCTC 1 cut(s) 849
BstNI CCWGG 1 cut(s) 308
BstNSI RCATGY 2 cut(s) 253, 550
BstSCI CCNGG 1 cut(s) 306
BstSFI CTRYAG 1 cut(s) 21
BstSLI GKGCMC 1 cut(s) 456
BstV1I GCAGC 1 cut(s) 537
BstV2I GAAGAC 1 cut(s) 384
BsuRI GGCC 1 cut(s) 568
BtgI CCRYGG 1 cut(s) 727
BtsCI GGATG 1 cut(s) 538
BtsI GCAGTG 2 cut(s) 210, 518
BtsIMutI CAGTG 2 cut(s) 210, 518
Cac8I GCNNGC 4 cut(s) 251, 497, 548, 566
Cfr10I RCCGGY 1 cut(s) 564
CsiI ACCWGGT 1 cut(s) 306
Csp6I GTAC 1 cut(s) 136
CviAII CATG 8 cut(s) 91, 239, 250, 388, 535, 543, 547, 728
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 4 cut(s) 284, 357, 593, 777
DraI TTTAAA 1 cut(s) 184
EaeI YGGCCR 1 cut(s) 566
Eco130I CCWWGG 1 cut(s) 727
Eco31I GGTCTC 1 cut(s) 849
Eco57I CTGAAG 1 cut(s) 687
EcoRI GAATTC 1 cut(s) 719
EcoRII CCWGG 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 727
EcoT22I ATGCAT 2 cut(s) 540, 548
ErhI CCWWGG 1 cut(s) 727
FaeI CATG 8 cut(s) 94, 242, 253, 391, 538, 546, 550, 731
FaqI GGGAC 1 cut(s) 829
FatI CATG 8 cut(s) 90, 238, 249, 387, 534, 542, 546, 727
FauI CCCGC 1 cut(s) 695
Fnu4HI GCNGC 1 cut(s) 551
FokI GGATG 1 cut(s) 525
Fsp4HI GCNGC 1 cut(s) 551
FspBI CTAG 2 cut(s) 41, 524
GluI GCNGC 1 cut(s) 551
GsaI CCCAGC 1 cut(s) 759
HaeIII GGCC 1 cut(s) 568
HapII CCGG 1 cut(s) 565
Hin1II CATG 8 cut(s) 94, 242, 253, 391, 538, 546, 550, 731
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HinfI GANTC 4 cut(s) 216, 262, 458, 773
HpaII CCGG 1 cut(s) 565
HphI GGTGA 4 cut(s) 523, 709, 743, 820
Hpy166II GTNNAC 3 cut(s) 165, 447, 620
Hpy188I TCNGA 7 cut(s) 120, 463, 585, 594, 628, 642, 778
Hpy188III TCNNGA 1 cut(s) 273
Hpy8I GTNNAC 3 cut(s) 165, 447, 620
HpyAV CCTTC 4 cut(s) 97, 662, 688, 728
HpyCH4III ACNGT 2 cut(s) 169, 451
HpyCH4V TGCA 7 cut(s) 203, 249, 354, 499, 538, 546, 553
HpyF3I CTNAG 4 cut(s) 284, 357, 593, 777
Hsp92II CATG 8 cut(s) 94, 242, 253, 391, 538, 546, 550, 731
KroI GCCGGC 1 cut(s) 564
KroNI GCCGGC 1 cut(s) 566
LmnI GCTCC 2 cut(s) 484, 607
Lsp1109I GCAGC 1 cut(s) 537
LweI GCATC 2 cut(s) 278, 547
MabI ACCWGGT 1 cut(s) 306
MaeI CTAG 2 cut(s) 41, 524
MaeIII GTNAC 2 cut(s) 419, 516
MboII GAAGA 2 cut(s) 384, 442
MfeI CAATTG 2 cut(s) 469, 788
MhlI GDGCHC 2 cut(s) 456, 604
MluCI AATT 8 cut(s) 50, 66, 425, 469, 554, 570, 719, 788
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 1 cut(s) 791
MnlI CCTC 5 cut(s) 125, 615, 753, 807, 844
Mph1103I ATGCAT 2 cut(s) 540, 548
MroNI GCCGGC 1 cut(s) 564
MseI TTAA 2 cut(s) 183, 324
MslI CAYNNNNRTG 1 cut(s) 729
MspI CCGG 1 cut(s) 565
MspR9I CCNGG 1 cut(s) 308
MunI CAATTG 2 cut(s) 469, 788
MvaI CCWGG 1 cut(s) 308
NaeI GCCGGC 1 cut(s) 566
NcoI CCATGG 1 cut(s) 727
NgoMIV GCCGGC 1 cut(s) 564
NlaIII CATG 8 cut(s) 94, 242, 253, 391, 538, 546, 550, 731
NlaIV GGNNCC 2 cut(s) 381, 480
NmuCI GTSAC 2 cut(s) 419, 516
NsiI ATGCAT 2 cut(s) 540, 548
NspI RCATGY 2 cut(s) 253, 550
OliI CACNNNNGTG 1 cut(s) 729
PaeI GCATGC 2 cut(s) 253, 550
PdiI GCCGGC 1 cut(s) 566
PfeI GAWTC 3 cut(s) 216, 262, 773
PkrI GCNGC 1 cut(s) 552
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
Psp6I CCWGG 1 cut(s) 306
PspFI CCCAGC 1 cut(s) 755
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 2 cut(s) 381, 480
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
RseI CAYNNNNRTG 1 cut(s) 729
SaqAI TTAA 2 cut(s) 183, 324
SatI GCNGC 1 cut(s) 551
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 1 cut(s) 308
SduI GDGCHC 2 cut(s) 456, 604
SetI ASST 9 cut(s) 309, 376, 415, 590, 640, 699, 745, 783, 833
SexAI ACCWGGT 1 cut(s) 306
SfaNI GCATC 2 cut(s) 278, 547
SfcI CTRYAG 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 729
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
SphI GCATGC 2 cut(s) 253, 550
Sse9I AATT 8 cut(s) 50, 66, 425, 469, 554, 570, 719, 788
SsiI CCGC 1 cut(s) 688
SspI AATATT 1 cut(s) 825
SspMI CTAG 2 cut(s) 41, 524
StyD4I CCNGG 1 cut(s) 306
StyI CCWWGG 1 cut(s) 727
TaaI ACNGT 2 cut(s) 169, 451
TaqI TCGA 1 cut(s) 529
TasI AATT 8 cut(s) 50, 66, 425, 469, 554, 570, 719, 788
TfiI GAWTC 3 cut(s) 216, 262, 773
Tru1I TTAA 2 cut(s) 183, 324
Tru9I TTAA 2 cut(s) 183, 324
TscAI CASTG 2 cut(s) 210, 525
TseFI GTSAC 2 cut(s) 419, 516
TseI GCWGC 1 cut(s) 550
Tsp45I GTSAC 2 cut(s) 419, 516
TspDTI ATGAA 3 cut(s) 79, 314, 672
TspRI CASTG 2 cut(s) 210, 525
XapI RAATTY 2 cut(s) 50, 719
XceI RCATGY 2 cut(s) 253, 550
XspI CTAG 2 cut(s) 41, 524
Zsp2I ATGCAT 2 cut(s) 540, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.