MD03G1273800.v1.1

histone H2A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002003
Physical Location & Seq
Forward (+)
35702610 .. 35703405
796 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1273800.v1.1.491

Sequence Viewer

Length: 426 bp
ATGAGCTCCACCGCTGAGACCACCACCAAAGCCGGCAGGGGCAAGCCCCGCACCACCAAGTCGGTCTCAAGATCCCAGAAGGCCGGCCTCCAATTCCCTGTCGGCCGAGTCGCCCGGTTCCTGAAGACCGGCCGCTACGCTCAGCGAGTCGGCGGTGGCTCCCCCGTCTACCTCTCCGCCGTCCTTGAGTACCTCGCCGCTGAGATTTTGGAGTTGGCCGGGAATGCGGCTAGGGACAACAAGAAGAACAGGATTACTCCTCGGCACATCCAGCTTGCCGTGCGAAACGACGAGGAGTTTACGAAGCTTTTGGGGTCTGTGACGATCGCCAACGGCGGCGTTCTGCCCAACATTCATCAGAATCTGCTTCCGAGCAAGGCCGGCAAGGGGAAGGGCGAGATTGGGTCTGCCTCCCAGGAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04217 ko05034 ko05322 map04217 map05034 map05322
Pfam Domains
Protein Families
Histone Histone_H2A_C

Protein Analysis

142

Amino Acids

15.03

Weight (kDa)

10.5

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 19 - 95 8.3e-15 Core histone H2A/H2B/H3/H4 domain
Histone_H2A_C PF16211 98 - 131 1.2e-16 C-terminus of histone H2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 168
AciI CCGC 8 cut(s) 12, 49, 133, 153, 177, 198, 227, 336
AclWI GGATC 1 cut(s) 66
AcoI YGGCCR 3 cut(s) 103, 130, 216
AcuI CTGAAG 1 cut(s) 143
AfaI GTAC 1 cut(s) 191
AfiI CCNNNNNNNGG 1 cut(s) 387
AjnI CCWGG 1 cut(s) 414
AluBI AGCT 3 cut(s) 6, 274, 307
AluI AGCT 3 cut(s) 6, 274, 307
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 2 cut(s) 11, 70
AlwI GGATC 1 cut(s) 66
AlwNI CAGNNNCTG 1 cut(s) 364
AoxI GGCC 6 cut(s) 81, 85, 103, 130, 216, 378
AsuC2I CCSGG 2 cut(s) 115, 220
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 131
Bbv12I GWGCWC 1 cut(s) 8
BceAI ACGGC 3 cut(s) 164, 263, 349
BciT130I CCWGG 1 cut(s) 416
BcnI CCSGG 2 cut(s) 115, 220
BcoDI GTCTC 2 cut(s) 11, 70
BfaI CTAG 1 cut(s) 231
BisI GCNGC 4 cut(s) 133, 198, 228, 337
BlpI GCTNAGC 1 cut(s) 141
BlsI GCNGC 4 cut(s) 134, 199, 229, 338
Bme1390I CCNGG 3 cut(s) 115, 220, 416
BmiI GGNNCC 2 cut(s) 119, 160
BmrFI CCNGG 3 cut(s) 115, 220, 416
BpiI GAAGAC 1 cut(s) 131
Bpu1102I GCTNAGC 1 cut(s) 141
BpuEI CTTGAG 2 cut(s) 52, 206
BpuMI CCSGG 2 cut(s) 115, 220
BsaI GGTCTC 2 cut(s) 11, 70
BsaJI CCNNGG 2 cut(s) 260, 414
Bsc4I CCNNNNNNNGG 1 cut(s) 387
Bse118I RCCGGY 4 cut(s) 32, 83, 128, 380
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 2 cut(s) 260, 414
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 1 cut(s) 387
BseMII CTCAG 3 cut(s) 6, 155, 192
BseRI GAGGAG 2 cut(s) 249, 308
BseX3I CGGCCG 2 cut(s) 103, 130
Bsh1285I CGRYCG 3 cut(s) 106, 133, 327
BshFI GGCC 6 cut(s) 83, 87, 105, 132, 218, 380
BsiEI CGRYCG 3 cut(s) 106, 133, 327
BsiHKAI GWGCWC 1 cut(s) 8
BsiSI CCGG 6 cut(s) 33, 84, 115, 129, 219, 381
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 1 cut(s) 387
BsmAI GTCTC 2 cut(s) 11, 70
BsmFI GGGAC 1 cut(s) 248
BsmI GAATGC 1 cut(s) 229
BsnI GGCC 6 cut(s) 83, 87, 105, 132, 218, 380
Bso31I GGTCTC 2 cut(s) 11, 70
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 2 cut(s) 71, 324
Bsp1720I GCTNAGC 1 cut(s) 141
BspACI CCGC 8 cut(s) 12, 49, 133, 153, 177, 198, 227, 336
BspANI GGCC 6 cut(s) 83, 87, 105, 132, 218, 380
BspCNI CTCAG 3 cut(s) 7, 154, 193
BspLI GGNNCC 2 cut(s) 119, 160
BspPI GGATC 1 cut(s) 66
BspTNI GGTCTC 2 cut(s) 11, 70
BsrFI RCCGGY 4 cut(s) 32, 83, 128, 380
BssAI RCCGGY 4 cut(s) 32, 83, 128, 380
BssECI CCNNGG 2 cut(s) 260, 414
BssMI GATC 2 cut(s) 71, 324
Bst2UI CCWGG 1 cut(s) 416
BstC8I GCNNGC 5 cut(s) 34, 44, 85, 276, 382
BstDEI CTNAG 3 cut(s) 15, 141, 201
BstF5I GGATG 1 cut(s) 267
BstKTI GATC 2 cut(s) 74, 327
BstMAI GTCTC 2 cut(s) 11, 70
BstMBI GATC 2 cut(s) 71, 324
BstMCI CGRYCG 3 cut(s) 106, 133, 327
BstMWI GCNNNNNNNGC 5 cut(s) 48, 224, 271, 280, 381
BstNI CCWGG 1 cut(s) 416
BstSCI CCNGG 3 cut(s) 113, 218, 414
BstV2I GAAGAC 1 cut(s) 131
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
BstZI CGGCCG 2 cut(s) 103, 130
BsuRI GGCC 6 cut(s) 83, 87, 105, 132, 218, 380
BtsCI GGATG 1 cut(s) 267
Cac8I GCNNGC 5 cut(s) 34, 44, 85, 276, 382
CaiI CAGNNNCTG 1 cut(s) 364
Cfr10I RCCGGY 4 cut(s) 32, 83, 128, 380
Csp6I GTAC 1 cut(s) 190
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 3 cut(s) 15, 141, 201
DpnI GATC 2 cut(s) 73, 326
DpnII GATC 2 cut(s) 71, 324
EaeI YGGCCR 3 cut(s) 103, 130, 216
EagI CGGCCG 2 cut(s) 103, 130
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 1 cut(s) 6
EclXI CGGCCG 2 cut(s) 103, 130
Eco24I GRGCYC 1 cut(s) 8
Eco31I GGTCTC 2 cut(s) 11, 70
Eco52I CGGCCG 2 cut(s) 103, 130
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 414
EcoT38I GRGCYC 1 cut(s) 8
FaqI GGGAC 1 cut(s) 248
FauI CCCGC 1 cut(s) 56
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 4 cut(s) 133, 198, 228, 337
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 8
FseI GGCCGGCC 1 cut(s) 87
Fsp4HI GCNGC 4 cut(s) 133, 198, 228, 337
FspBI CTAG 1 cut(s) 231
GluI GCNGC 4 cut(s) 133, 198, 228, 337
HaeIII GGCC 6 cut(s) 83, 87, 105, 132, 218, 380
HapII CCGG 6 cut(s) 33, 84, 115, 129, 219, 381
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 3 cut(s) 108, 147, 361
HpaII CCGG 6 cut(s) 33, 84, 115, 129, 219, 381
Hpy166II GTNNAC 2 cut(s) 169, 300
Hpy188I TCNGA 2 cut(s) 360, 372
Hpy188III TCNNGA 2 cut(s) 69, 121
Hpy8I GTNNAC 2 cut(s) 169, 300
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 2 cut(s) 73, 385
HpyF10VI GCNNNNNNNGC 5 cut(s) 48, 224, 271, 280, 381
HpyF3I CTNAG 3 cut(s) 15, 141, 201
KroI GCCGGC 3 cut(s) 32, 83, 380
KroNI GCCGGC 3 cut(s) 34, 85, 382
Kzo9I GATC 2 cut(s) 71, 324
LmnI GCTCC 2 cut(s) 11, 164
MaeI CTAG 1 cut(s) 231
MaeIII GTNAC 1 cut(s) 319
MalI GATC 2 cut(s) 73, 326
MboI GATC 2 cut(s) 71, 324
MboII GAAGA 2 cut(s) 136, 256
MflI RGATCY 1 cut(s) 71
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 1 cut(s) 92
MlyI GAGTC 2 cut(s) 117, 156
MnlI CCTC 6 cut(s) 98, 182, 203, 270, 286, 421
MroNI GCCGGC 3 cut(s) 32, 83, 380
MspA1I CMGCKG 2 cut(s) 14, 200
MspI CCGG 6 cut(s) 33, 84, 115, 129, 219, 381
MspR9I CCNGG 3 cut(s) 115, 220, 416
Mva1269I GAATGC 1 cut(s) 229
MvaI CCWGG 1 cut(s) 416
MwoI GCNNNNNNNGC 5 cut(s) 48, 224, 271, 280, 381
NaeI GCCGGC 3 cut(s) 34, 85, 382
NciI CCSGG 2 cut(s) 115, 220
NdeII GATC 2 cut(s) 71, 324
NgoMIV GCCGGC 3 cut(s) 32, 83, 380
NlaIV GGNNCC 2 cut(s) 119, 160
NmeAIII GCCGAG 2 cut(s) 131, 241
NmuCI GTSAC 1 cut(s) 319
PctI GAATGC 1 cut(s) 229
PdiI GCCGGC 3 cut(s) 34, 85, 382
PfeI GAWTC 1 cut(s) 361
PkrI GCNGC 4 cut(s) 134, 199, 229, 338
Ple19I CGATCG 1 cut(s) 327
PleI GAGTC 2 cut(s) 116, 155
PpsI GAGTC 2 cut(s) 116, 155
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 414
PspGI CCWGG 1 cut(s) 414
PspN4I GGNNCC 2 cut(s) 119, 160
PstNI CAGNNNCTG 1 cut(s) 364
PsuI RGATCY 1 cut(s) 71
PvuI CGATCG 1 cut(s) 327
RigI GGCCGGCC 1 cut(s) 87
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
SacI GAGCTC 1 cut(s) 8
SatI GCNGC 4 cut(s) 133, 198, 228, 337
Sau3AI GATC 2 cut(s) 71, 324
SchI GAGTC 2 cut(s) 117, 156
ScrFI CCNGG 3 cut(s) 115, 220, 416
SduI GDGCHC 1 cut(s) 8
SetI ASST 5 cut(s) 8, 174, 195, 276, 309
SmlI CTYRAG 2 cut(s) 67, 185
SmoI CTYRAG 2 cut(s) 67, 185
Sse9I AATT 1 cut(s) 92
SsiI CCGC 8 cut(s) 12, 49, 133, 153, 177, 198, 227, 336
SspMI CTAG 1 cut(s) 231
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 113, 218, 414
TaqII GACCGA 1 cut(s) 52
TasI AATT 1 cut(s) 92
TauI GCSGC 4 cut(s) 135, 200, 230, 339
TfiI GAWTC 1 cut(s) 361
TseFI GTSAC 1 cut(s) 319
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.