Rorug06G0225000

histone H2A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002003
Physical Location & Seq
Reverse (-)
36059839 .. 36062442
2604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0225000.1

Sequence Viewer

Length: 1308 bp
ATGGAGGTGACTATTATTTCCAGAGATACCATCATACCAGCCACTACTTCCTCTTCAGTTCATCGCCCTAAGAAACCTTACCAACTTTGTATCTTCGATCAGCTCTTTCCCATAACTTACGTTCCATTGGTTTTCTTCTACCCCATTTTTGACCCAAATATCAATGTTTCCCAAACCCTAGCCCACCTGAAAAGCTCCCTATCATATACACTCGTTGTGTACTACCCATTTTCCGGACGAATAAAAAACAACCTTTATATTGATGATTTTGATGCGGGTGTCCCCTACCTTGAAGCCCGAATCAATTATCGCCTGTTTGAGTTTCTTAAGCTCCATGAAATTGAGCTACTCAATCAGTTCGTTCCATTCCATCCATTGCGTCAGGAAACTGAATCTCATGATGATCAGCTGCCCTTATTAGCAATTCAAGTCAGTGTTTTTTCGTGTGGTGGAATAGCAATTGGGGTGTCCGGATCTCACAAGATCGTTGATGGGGACACGGCAAACAATTTCCTCAAATCTTGGGCTGCTGTTTTTCGTGGGGATCATGACAAAATTCTGCTGCACACTAATCTCTACCAAGCTTCATTAAGTTTTCCAGCAAAAGATGACATACCAGAAAAATATCTGGCTTTCACGGATAGCATGGTGTTCAGCGGAAAACATTATATCACAAGGAGATTTGTGTTTAATGCTAAAGCCATAGACATTCTACGAGACAAAGCAAAGAGCGAGGTCGTGCCCAATCCAACACGTAGTACTTCACGAAGAGCATCAACAGCAGTGCATGCAGTGAACATAAGGCCGCAATTGAAGCTCTCGATGTTAGATAACGCCATTGGAAATATTTTTTGGTGGGCAATAGCGGCTAACATCCCTACTAATAAGGGAGAAACTGGTGATGATGATCAGTTGTGTGATCTGATGAAGCTTTTACATGAATCTATCAAAGGATTGGACAATGACTATTTGAAGACATTGCAGAGAGAAGAGGGATTTGGAGCCATTTCTAAGTTCATCGATCGCCATTTAGAGGTTATATATTCTTTGAAACCGGAAACATATATATTCACCAGCTGGACTAAGATTTTCAAGGAAGTTGATTTTGGATGGGGGAGACCGTTTTGGATTGGGGTCATGGGGAAAGTAGGACCAACTCTTGGCAATCTCACGGTGTTCGTTGAAACACAATGGGGTAAGGGCATAGAAGCATGGGTGACCTTGGAGGAAAAGCAAATGGCTGTAATGGAAAATGATCCTCAGTTTTTAGCATTTACTTCTCCAAACCCACGCCTTTCAAGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04217 ko05034 ko05322 map04217 map05034 map05322
Pfam Domains
Protein Families
Histone Histone_H2A_C

Protein Analysis

435

Amino Acids

49.46

Weight (kDa)

6.32

Isoelectric Point (pI)

32.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 422 5.7e-59 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1160
AccIII TCCGGA 2 cut(s) 233, 470
AciI CCGC 4 cut(s) 275, 657, 806, 866
AclWI GGATC 3 cut(s) 481, 552, 1250
AcsI RAATTY 1 cut(s) 555
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 2 cut(s) 221, 760
AfiI CCNNNNNNNGG 3 cut(s) 233, 1033, 1160
AflII CTTAAG 1 cut(s) 326
AflIII ACRYGT 1 cut(s) 752
AgsI TTSAA 8 cut(s) 293, 428, 814, 973, 1051, 1093, 1184, 1299
AjuI GAANNNNNNNTTGG 6 cut(s) 835, 867, 981, 1013, 1089, 1121
AluBI AGCT 9 cut(s) 103, 195, 331, 346, 409, 584, 817, 931, 1077
AluI AGCT 9 cut(s) 103, 195, 331, 346, 409, 584, 817, 931, 1077
Alw26I GTCTC 2 cut(s) 711, 1111
AlwI GGATC 3 cut(s) 481, 552, 1250
Aor13HI TCCGGA 2 cut(s) 233, 470
AoxI GGCC 1 cut(s) 803
ApeKI GCWGC 3 cut(s) 409, 527, 562
ApoI RAATTY 1 cut(s) 555
AspS9I GGNCC 1 cut(s) 1151
AsuHPI GGTGA 4 cut(s) 19, 911, 1063, 1228
AvaII GGWCC 1 cut(s) 1151
BaeGI GKGCMC 1 cut(s) 744
BbsI GAAGAC 1 cut(s) 980
BbvI GCAGC 3 cut(s) 396, 514, 549
BccI CCATC 4 cut(s) 38, 378, 485, 1104
BceAI ACGGC 1 cut(s) 516
BclI TGATCA 2 cut(s) 403, 907
BcoDI GTCTC 2 cut(s) 711, 1111
BfaI CTAG 1 cut(s) 179
BfrI CTTAAG 1 cut(s) 326
BisI GCNGC 5 cut(s) 410, 528, 563, 806, 867
BlsI GCNGC 5 cut(s) 411, 529, 564, 807, 868
BmcAI AGTACT 1 cut(s) 760
Bme18I GGWCC 1 cut(s) 1151
BmgT120I GGNCC 1 cut(s) 1151
BmiI GGNNCC 1 cut(s) 1003
BmsI GCATC 2 cut(s) 262, 782
BpiI GAAGAC 1 cut(s) 980
Bsa29I ATCGAT 1 cut(s) 1020
BsaAI YACGTR 1 cut(s) 755
BsaBI GATNNNNATC 1 cut(s) 906
BsaI GGTCTC 1 cut(s) 1111
BsaJI CCNNGG 1 cut(s) 1221
BsaWI WCCGGW 3 cut(s) 233, 470, 1054
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 1033, 1160
Bse1I ACTGG 1 cut(s) 901
Bse3DI GCAATG 2 cut(s) 374, 977
Bse8I GATNNNNATC 1 cut(s) 906
BseAI TCCGGA 2 cut(s) 233, 470
BseCI ATCGAT 1 cut(s) 1020
BseDI CCNNGG 1 cut(s) 1221
BseGI GGATG 3 cut(s) 370, 873, 1115
BseJI GATNNNNATC 1 cut(s) 906
BseLI CCNNNNNNNGG 3 cut(s) 233, 1033, 1160
BseMI GCAATG 2 cut(s) 374, 977
BseMII CTCAG 1 cut(s) 1274
BseNI ACTGG 1 cut(s) 901
BseSI GKGCMC 1 cut(s) 744
BseXI GCAGC 3 cut(s) 396, 514, 549
BsgI GTGCAG 1 cut(s) 548
Bsh1285I CGRYCG 1 cut(s) 1024
BshFI GGCC 1 cut(s) 805
BshVI ATCGAT 1 cut(s) 1020
BsiEI CGRYCG 1 cut(s) 1024
BsiSI CCGG 3 cut(s) 234, 471, 1055
BslFI GGGAC 2 cut(s) 266, 509
BslI CCNNNNNNNGG 3 cut(s) 233, 1033, 1160
BsmAI GTCTC 2 cut(s) 711, 1111
BsmFI GGGAC 2 cut(s) 266, 509
BsnI GGCC 1 cut(s) 805
Bso31I GGTCTC 1 cut(s) 1111
Bsp1286I GDGCHC 1 cut(s) 744
Bsp13I TCCGGA 2 cut(s) 233, 470
Bsp143I GATC 9 cut(s) 97, 403, 473, 483, 544, 907, 919, 1021, 1255
BspACI CCGC 4 cut(s) 275, 657, 806, 866
BspANI GGCC 1 cut(s) 805
BspCNI CTCAG 1 cut(s) 1273
BspDI ATCGAT 1 cut(s) 1020
BspEI TCCGGA 2 cut(s) 233, 470
BspHI TCATGA 2 cut(s) 397, 547
BspLI GGNNCC 1 cut(s) 1003
BspPI GGATC 3 cut(s) 481, 552, 1250
BspQI GCTCTTC 1 cut(s) 763
BspTI CTTAAG 1 cut(s) 326
BspTNI GGTCTC 1 cut(s) 1111
BsrDI GCAATG 2 cut(s) 374, 977
BsrI ACTGG 1 cut(s) 901
BssECI CCNNGG 1 cut(s) 1221
BssMI GATC 9 cut(s) 97, 403, 473, 483, 544, 907, 919, 1021, 1255
BssT1I CCWWGG 1 cut(s) 1221
Bst4CI ACNGT 2 cut(s) 1122, 1174
Bst6I CTCTTC 3 cut(s) 58, 763, 984
BstAFI CTTAAG 1 cut(s) 326
BstAPI GCANNNNNTGC 1 cut(s) 788
BstBAI YACGTR 1 cut(s) 755
BstC8I GCNNGC 1 cut(s) 789
BstDEI CTNAG 4 cut(s) 69, 1011, 1083, 1260
BstEII GGTNACC 1 cut(s) 1216
BstF5I GGATG 3 cut(s) 370, 873, 1115
BstKTI GATC 9 cut(s) 100, 406, 476, 486, 547, 910, 922, 1024, 1258
BstMAI GTCTC 2 cut(s) 711, 1111
BstMBI GATC 9 cut(s) 97, 403, 473, 483, 544, 907, 919, 1021, 1255
BstMCI CGRYCG 1 cut(s) 1024
BstMWI GCNNNNNNNGC 5 cut(s) 779, 788, 814, 866, 1299
BstNSI RCATGY 2 cut(s) 791, 1306
BstPI GGTNACC 1 cut(s) 1216
BstSLI GKGCMC 1 cut(s) 744
BstV1I GCAGC 3 cut(s) 396, 514, 549
BstV2I GAAGAC 1 cut(s) 980
BstX2I RGATCY 1 cut(s) 473
BstYI RGATCY 1 cut(s) 473
Bsu15I ATCGAT 1 cut(s) 1020
BsuRI GGCC 1 cut(s) 805
BsuTUI ATCGAT 1 cut(s) 1020
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 3 cut(s) 370, 873, 1115
BtsI GCAGTG 2 cut(s) 789, 798
BtsIMutI CAGTG 3 cut(s) 439, 789, 798
Cac8I GCNNGC 1 cut(s) 789
CciI TCATGA 2 cut(s) 397, 547
Cfr13I GGNCC 1 cut(s) 1151
ClaI ATCGAT 1 cut(s) 1020
CseI GACGC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 220, 759
CviAII CATG 9 cut(s) 335, 398, 548, 646, 788, 938, 1138, 1212, 1303
CviQI GTAC 2 cut(s) 220, 759
DdeI CTNAG 4 cut(s) 69, 1011, 1083, 1260
DpnI GATC 9 cut(s) 99, 405, 475, 485, 546, 909, 921, 1023, 1257
DpnII GATC 9 cut(s) 97, 403, 473, 483, 544, 907, 919, 1021, 1255
Eam1104I CTCTTC 3 cut(s) 58, 763, 984
EarI CTCTTC 3 cut(s) 58, 763, 984
Eco130I CCWWGG 1 cut(s) 1221
Eco31I GGTCTC 1 cut(s) 1111
Eco47I GGWCC 1 cut(s) 1151
Eco57I CTGAAG 1 cut(s) 39
Eco91I GGTNACC 1 cut(s) 1216
EcoO65I GGTNACC 1 cut(s) 1216
EcoT14I CCWWGG 1 cut(s) 1221
ErhI CCWWGG 1 cut(s) 1221
FaeI CATG 9 cut(s) 338, 401, 551, 649, 791, 941, 1141, 1215, 1306
FaqI GGGAC 2 cut(s) 266, 509
FatI CATG 9 cut(s) 334, 397, 547, 645, 787, 937, 1137, 1211, 1302
FauI CCCGC 1 cut(s) 268
FbaI TGATCA 2 cut(s) 403, 907
Fnu4HI GCNGC 5 cut(s) 410, 528, 563, 806, 867
FokI GGATG 3 cut(s) 357, 860, 1122
Fsp4HI GCNGC 5 cut(s) 410, 528, 563, 806, 867
FspBI CTAG 1 cut(s) 179
GluI GCNGC 5 cut(s) 410, 528, 563, 806, 867
HaeIII GGCC 1 cut(s) 805
HapII CCGG 3 cut(s) 234, 471, 1055
HgaI GACGC 1 cut(s) 368
Hin1II CATG 9 cut(s) 338, 401, 551, 649, 791, 941, 1141, 1215, 1306
HindIII AAGCTT 2 cut(s) 582, 929
HinfI GANTC 3 cut(s) 300, 392, 941
HpaII CCGG 3 cut(s) 234, 471, 1055
HphI GGTGA 4 cut(s) 19, 911, 1063, 1228
Hpy166II GTNNAC 2 cut(s) 220, 796
Hpy188I TCNGA 1 cut(s) 924
Hpy188III TCNNGA 8 cut(s) 21, 234, 383, 398, 471, 548, 765, 820
Hpy8I GTNNAC 2 cut(s) 220, 796
HpyCH4III ACNGT 2 cut(s) 1122, 1174
HpyCH4IV ACGT 2 cut(s) 120, 754
HpyCH4V TGCA 4 cut(s) 565, 787, 791, 982
HpyF10VI GCNNNNNNNGC 5 cut(s) 779, 788, 814, 866, 1299
HpyF3I CTNAG 4 cut(s) 69, 1011, 1083, 1260
HpySE526I ACGT 2 cut(s) 120, 754
Hsp92II CATG 9 cut(s) 338, 401, 551, 649, 791, 941, 1141, 1215, 1306
Kpn2I TCCGGA 2 cut(s) 233, 470
Ksp22I TGATCA 2 cut(s) 403, 907
Kzo9I GATC 9 cut(s) 97, 403, 473, 483, 544, 907, 919, 1021, 1255
LguI GCTCTTC 1 cut(s) 763
LmnI GCTCC 3 cut(s) 200, 336, 1001
Lsp1109I GCAGC 3 cut(s) 396, 514, 549
LweI GCATC 2 cut(s) 262, 782
MaeI CTAG 1 cut(s) 179
MaeII ACGT 2 cut(s) 120, 754
MaeIII GTNAC 2 cut(s) 7, 1216
MalI GATC 9 cut(s) 99, 405, 475, 485, 546, 909, 921, 1023, 1257
MboI GATC 9 cut(s) 97, 403, 473, 483, 544, 907, 919, 1021, 1255
MboII GAAGA 6 cut(s) 45, 85, 127, 780, 985, 1001
MfeI CAATTG 2 cut(s) 459, 809
MflI RGATCY 1 cut(s) 473
MhlI GDGCHC 1 cut(s) 744
MluCI AATT 7 cut(s) 304, 339, 423, 459, 508, 555, 809
MmeI TCCRAC 1 cut(s) 773
MnlI CCTC 7 cut(s) 61, 524, 727, 985, 1027, 1219, 1269
MroI TCCGGA 2 cut(s) 233, 470
MseI TTAA 3 cut(s) 327, 590, 690
MspA1I CMGCKG 3 cut(s) 409, 657, 1077
MspCI CTTAAG 1 cut(s) 326
MspI CCGG 3 cut(s) 234, 471, 1055
MunI CAATTG 2 cut(s) 459, 809
MwoI GCNNNNNNNGC 5 cut(s) 779, 788, 814, 866, 1299
NdeII GATC 9 cut(s) 97, 403, 473, 483, 544, 907, 919, 1021, 1255
NlaIII CATG 9 cut(s) 338, 401, 551, 649, 791, 941, 1141, 1215, 1306
NlaIV GGNNCC 1 cut(s) 1003
NmuCI GTSAC 2 cut(s) 7, 1216
NspI RCATGY 2 cut(s) 791, 1306
PaeI GCATGC 1 cut(s) 791
PagI TCATGA 2 cut(s) 397, 547
PciSI GCTCTTC 1 cut(s) 763
PfeI GAWTC 3 cut(s) 300, 392, 941
PflMI CCANNNNNTGG 1 cut(s) 1160
PkrI GCNGC 5 cut(s) 411, 529, 564, 807, 868
Ple19I CGATCG 1 cut(s) 1024
Ppu21I YACGTR 1 cut(s) 755
PspEI GGTNACC 1 cut(s) 1216
PspN4I GGNNCC 1 cut(s) 1003
PspPI GGNCC 1 cut(s) 1151
PsuI RGATCY 1 cut(s) 473
PvuI CGATCG 1 cut(s) 1024
PvuII CAGCTG 2 cut(s) 409, 1077
RsaI GTAC 2 cut(s) 221, 760
RsaNI GTAC 2 cut(s) 220, 759
SapI GCTCTTC 1 cut(s) 763
SaqAI TTAA 3 cut(s) 327, 590, 690
SatI GCNGC 5 cut(s) 410, 528, 563, 806, 867
Sau3AI GATC 9 cut(s) 97, 403, 473, 483, 544, 907, 919, 1021, 1255
Sau96I GGNCC 1 cut(s) 1151
ScaI AGTACT 1 cut(s) 760
SduI GDGCHC 1 cut(s) 744
SfaNI GCATC 2 cut(s) 262, 782
SinI GGWCC 1 cut(s) 1151
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SphI GCATGC 1 cut(s) 791
Sse9I AATT 7 cut(s) 304, 339, 423, 459, 508, 555, 809
SsiI CCGC 4 cut(s) 275, 657, 806, 866
SspI AATATT 1 cut(s) 847
SspMI CTAG 1 cut(s) 179
StyI CCWWGG 1 cut(s) 1221
TaaI ACNGT 2 cut(s) 1122, 1174
TaiI ACGT 2 cut(s) 123, 757
TaqI TCGA 3 cut(s) 96, 821, 1020
TasI AATT 7 cut(s) 304, 339, 423, 459, 508, 555, 809
TatI WGTACW 2 cut(s) 219, 758
TauI GCSGC 2 cut(s) 808, 869
TfiI GAWTC 3 cut(s) 300, 392, 941
Tru1I TTAA 3 cut(s) 327, 590, 690
Tru9I TTAA 3 cut(s) 327, 590, 690
TscAI CASTG 3 cut(s) 439, 789, 798
TseFI GTSAC 2 cut(s) 7, 1216
TseI GCWGC 3 cut(s) 409, 527, 562
Tsp45I GTSAC 2 cut(s) 7, 1216
TspDTI ATGAA 6 cut(s) 50, 351, 576, 941, 954, 1006
TspGWI ACGGA 1 cut(s) 653
TspRI CASTG 3 cut(s) 439, 789, 798
Van91I CCANNNNNTGG 1 cut(s) 1160
Vha464I CTTAAG 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 1151
XapI RAATTY 1 cut(s) 555
XceI RCATGY 2 cut(s) 791, 1306
XspI CTAG 1 cut(s) 179
ZrmI AGTACT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.