Rw6G029440

histone H2A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002003
Physical Location & Seq
Forward (+)
53574773 .. 53575295
523 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G029440.1

Sequence Viewer

Length: 426 bp
ATGACCACCGCCGTCGCCACAACAGCTCAGGCCGGCCGAGGCAAGCCCAAGGCCACCAAATCCGTCACCAGGTCATCCAAGGCCGGCCTCCAATTCCCCGTCGGTCGAGTCGCCCGCTTCCTCAAGAAGGGCCGCTACGCCCAGCGCGTTGGCGGTGGCTCCCCCGTCTACCTCTCCGCCGTCCTCGAATACCTAGCTGCTGAGGTTTTGGAGCTGGCTGGAAACGCTGCCAGGGACAACAAGAAGAATCGGATCACTCCGAGGCATATCCAGCTTGCGGTGAGGAATGACGAGGAGTTCACCAAGCTTTTGGGGTCTGTGACGATTGCGAATGGAGGTGTTCTGCCCAACATTCATCAGAACTTGTTGCCAAAGAAGAATGCAAAAGGGAAGGGCGAGATTGGATCTATGTCTCAGGAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04217 ko05034 ko05322 map04217 map05034 map05322
Pfam Domains
Protein Families
Histone Histone_H2A_C

Protein Analysis

141

Amino Acids

15.06

Weight (kDa)

10.58

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 17 - 95 9e-15 Core histone H2A/H2B/H3/H4 domain
Histone_H2A_C PF16211 98 - 131 2.1e-17 C-terminus of histone H2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 168
AccII CGCG 1 cut(s) 147
AciI CCGC 6 cut(s) 9, 115, 133, 153, 177, 278
AclWI GGATC 2 cut(s) 260, 412
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 1 cut(s) 419
AfiI CCNNNNNNNGG 3 cut(s) 69, 127, 277
AjnI CCWGG 2 cut(s) 68, 230
AluBI AGCT 5 cut(s) 26, 197, 214, 274, 307
AluI AGCT 5 cut(s) 26, 197, 214, 274, 307
Alw26I GTCTC 1 cut(s) 417
AlwI GGATC 2 cut(s) 260, 412
AoxI GGCC 6 cut(s) 30, 34, 51, 81, 85, 130
ApeKI GCWGC 2 cut(s) 197, 227
ApoI RAATTY 1 cut(s) 419
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 1 cut(s) 130
AsuHPI GGTGA 3 cut(s) 58, 292, 292
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 2 cut(s) 184, 214
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 2 cut(s) 70, 232
BcoDI GTCTC 1 cut(s) 417
BfaI CTAG 2 cut(s) 194, 424
BisI GCNGC 3 cut(s) 133, 198, 228
BlsI GCNGC 3 cut(s) 134, 199, 229
Bme1390I CCNGG 2 cut(s) 70, 232
BmgT120I GGNCC 1 cut(s) 130
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 2 cut(s) 70, 232
Bpu10I CCTNAGC 2 cut(s) 27, 201
BpuEI CTTGAG 1 cut(s) 107
BsaJI CCNNGG 5 cut(s) 37, 48, 78, 231, 260
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 127, 277
Bse118I RCCGGY 2 cut(s) 32, 83
BseBI CCWGG 2 cut(s) 70, 232
BseDI CCNNGG 5 cut(s) 37, 48, 78, 231, 260
BseGI GGATG 1 cut(s) 74
BseLI CCNNNNNNNGG 3 cut(s) 69, 127, 277
BseMII CTCAG 2 cut(s) 41, 192
BseRI GAGGAG 1 cut(s) 308
BseX3I CGGCCG 1 cut(s) 34
BseXI GCAGC 2 cut(s) 184, 214
BseYI CCCAGC 1 cut(s) 141
Bsh1236I CGCG 1 cut(s) 147
Bsh1285I CGRYCG 2 cut(s) 37, 106
BshFI GGCC 6 cut(s) 32, 36, 53, 83, 87, 132
BsiEI CGRYCG 2 cut(s) 37, 106
BsiSI CCGG 2 cut(s) 33, 84
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 3 cut(s) 69, 127, 277
BsmAI GTCTC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 248
BsmI GAATGC 1 cut(s) 385
BsnI GGCC 6 cut(s) 32, 36, 53, 83, 87, 132
Bsp143I GATC 2 cut(s) 252, 404
BspACI CCGC 6 cut(s) 9, 115, 133, 153, 177, 278
BspANI GGCC 6 cut(s) 32, 36, 53, 83, 87, 132
BspCNI CTCAG 2 cut(s) 40, 193
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 160
BspPI GGATC 2 cut(s) 260, 412
BsrFI RCCGGY 2 cut(s) 32, 83
BssAI RCCGGY 2 cut(s) 32, 83
BssECI CCNNGG 5 cut(s) 37, 48, 78, 231, 260
BssMI GATC 2 cut(s) 252, 404
BssT1I CCWWGG 2 cut(s) 48, 78
Bst2UI CCWGG 2 cut(s) 70, 232
BstC8I GCNNGC 6 cut(s) 34, 44, 85, 115, 216, 276
BstDEI CTNAG 3 cut(s) 27, 201, 414
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 1 cut(s) 74
BstFNI CGCG 1 cut(s) 147
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 2 cut(s) 255, 407
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 2 cut(s) 252, 404
BstMCI CGRYCG 2 cut(s) 37, 106
BstMWI GCNNNNNNNGC 3 cut(s) 23, 224, 271
BstNI CCWGG 2 cut(s) 70, 232
BstSCI CCNGG 2 cut(s) 68, 230
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 2 cut(s) 184, 214
BstX2I RGATCY 1 cut(s) 404
BstXI CCANNNNNNTGG 2 cut(s) 149, 310
BstYI RGATCY 1 cut(s) 404
BstZI CGGCCG 1 cut(s) 34
BsuRI GGCC 6 cut(s) 32, 36, 53, 83, 87, 132
BtsCI GGATG 1 cut(s) 74
Cac8I GCNNGC 6 cut(s) 34, 44, 85, 115, 216, 276
CfoI GCGC 1 cut(s) 147
Cfr10I RCCGGY 2 cut(s) 32, 83
Cfr13I GGNCC 1 cut(s) 130
CsiI ACCWGGT 1 cut(s) 68
DdeI CTNAG 3 cut(s) 27, 201, 414
DpnI GATC 2 cut(s) 254, 406
DpnII GATC 2 cut(s) 252, 404
EaeI YGGCCR 1 cut(s) 34
EagI CGGCCG 1 cut(s) 34
EciI GGCGGA 1 cut(s) 166
EclXI CGGCCG 1 cut(s) 34
Eco130I CCWWGG 2 cut(s) 48, 78
Eco52I CGGCCG 1 cut(s) 34
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoRI GAATTC 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 68, 230
EcoT14I CCWWGG 2 cut(s) 48, 78
ErhI CCWWGG 2 cut(s) 48, 78
FaiI YATR 2 cut(s) 267, 410
FaqI GGGAC 1 cut(s) 248
FauI CCCGC 1 cut(s) 122
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 133, 198, 228
FokI GGATG 1 cut(s) 61
FseI GGCCGGCC 2 cut(s) 36, 87
Fsp4HI GCNGC 3 cut(s) 133, 198, 228
FspBI CTAG 2 cut(s) 194, 424
GlaI GCGC 1 cut(s) 146
GluI GCNGC 3 cut(s) 133, 198, 228
GsaI CCCAGC 1 cut(s) 145
HaeIII GGCC 6 cut(s) 32, 36, 53, 83, 87, 132
HapII CCGG 2 cut(s) 33, 84
HhaI GCGC 1 cut(s) 147
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 2 cut(s) 108, 247
HpaII CCGG 2 cut(s) 33, 84
HphI GGTGA 3 cut(s) 58, 292, 292
Hpy166II GTNNAC 2 cut(s) 169, 300
Hpy188I TCNGA 3 cut(s) 252, 261, 360
Hpy188III TCNNGA 2 cut(s) 124, 416
Hpy8I GTNNAC 2 cut(s) 169, 300
Hpy99I CGWCG 2 cut(s) 17, 104
HpyAV CCTTC 2 cut(s) 121, 385
HpyCH4V TGCA 1 cut(s) 383
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 224, 271
HpyF3I CTNAG 3 cut(s) 27, 201, 414
HspAI GCGC 1 cut(s) 145
KroI GCCGGC 2 cut(s) 32, 83
KroNI GCCGGC 2 cut(s) 34, 85
Kzo9I GATC 2 cut(s) 252, 404
LmnI GCTCC 2 cut(s) 164, 211
Lsp1109I GCAGC 2 cut(s) 184, 214
MabI ACCWGGT 1 cut(s) 68
MaeI CTAG 2 cut(s) 194, 424
MaeIII GTNAC 2 cut(s) 64, 319
MalI GATC 2 cut(s) 254, 406
MboI GATC 2 cut(s) 252, 404
MboII GAAGA 2 cut(s) 256, 388
MflI RGATCY 1 cut(s) 404
MluCI AATT 2 cut(s) 92, 419
MlyI GAGTC 1 cut(s) 117
MroNI GCCGGC 2 cut(s) 32, 83
MspI CCGG 2 cut(s) 33, 84
MspR9I CCNGG 2 cut(s) 70, 232
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 2 cut(s) 70, 232
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 3 cut(s) 23, 224, 271
NaeI GCCGGC 2 cut(s) 34, 85
NdeII GATC 2 cut(s) 252, 404
NgoMIV GCCGGC 2 cut(s) 32, 83
NlaIV GGNNCC 1 cut(s) 160
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 2 cut(s) 64, 319
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PctI GAATGC 1 cut(s) 385
PdiI GCCGGC 2 cut(s) 34, 85
PfeI GAWTC 1 cut(s) 247
PkrI GCNGC 3 cut(s) 134, 199, 229
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
Psp6I CCWGG 2 cut(s) 68, 230
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 2 cut(s) 68, 230
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 1 cut(s) 130
PsuI RGATCY 1 cut(s) 404
RigI GGCCGGCC 2 cut(s) 36, 87
SatI GCNGC 3 cut(s) 133, 198, 228
Sau3AI GATC 2 cut(s) 252, 404
Sau96I GGNCC 1 cut(s) 130
SchI GAGTC 1 cut(s) 117
ScrFI CCNGG 2 cut(s) 70, 232
SexAI ACCWGGT 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 2 cut(s) 92, 419
SsiI CCGC 6 cut(s) 9, 115, 133, 153, 177, 278
SspMI CTAG 2 cut(s) 194, 424
StyD4I CCNGG 2 cut(s) 68, 230
StyI CCWWGG 2 cut(s) 48, 78
TaqI TCGA 2 cut(s) 106, 186
TaqII GACCGA 1 cut(s) 92
TasI AATT 2 cut(s) 92, 419
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 1 cut(s) 247
TseFI GTSAC 2 cut(s) 64, 319
TseI GCWGC 2 cut(s) 197, 227
Tsp45I GTSAC 2 cut(s) 64, 319
TspDTI ATGAA 1 cut(s) 344
TspGWI ACGGA 1 cut(s) 52
XagI CCTNNNNNAGG 1 cut(s) 125
XapI RAATTY 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 2 cut(s) 194, 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.