MD04G1012300.v1.1

TBC1 domain family member

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
Physical Location & Seq
Forward (+)
1388505 .. 1393112
4608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1012300.v1.1.491

Sequence Viewer

Length: 1395 bp
ATGTTTCACTCACCTCAAATGGTGAAGAAGCGAGTCCCCGAATGGCTCAACAGCTCGCTCTGGTCCTCCTCCACGCCTTCTTCTTCCATCGACGACGATCGCGCCCAGCGCTACACCTCCAATCCCACCGCCGCCGGCCCCACCACCGAAATTTCAAGTTCTTCTGCCTCAGACTCTGAGCCGGCTTCCACGGTTCACCCGCCAATCCCTGTTGCTCCTCCCAAATCCACCAGGGAAGACAAGCCTCCTCCCGAGCCCCAATTCACAGATTCATTTCCGGATCACGAAAACAAGAGCAATAACACCGCTCCGTCCCCCGAAGATATCTCCAGACAATCTCAGCTCTTGGCCGAGCTGTCGAGGAAGGTGATTGATTTGCGCGAATTGCAGAGAATTTCATCGCAGGGCATACCAGATGGTGCTGGGATACGGTCCACTGTCTGGAAGCTTTTACTTGGATATCTACCACCAGATCGGGGGCTTTGGTCGACTGAGTTAGCTAAAAAGAGGTCACAGTACAAGCATTTTAAAGATGAGCTTTTGATGAATCCTTCAGAAATCACGAGGAAGATGGATAATGGTGCTACTTGTGACAAGGATGAATTAAAATGTCAAAGCAAGGGGTTGCTATCAAGATCAGAAATACCTCATGGGGAGCATCCATTGAGTCTTGGGAAGTCCAGCGTCTGGAATCAGTTCTTTCAGGACACAGAGATCATAGAACAAATCGACCGAGATGTAAAGCGTACTCATCCAGACATGCATTTTTTCTCAGGGGACTCTCAAGATGCTAAATCTAATCAGGATGCTTTGAAGGACATCCTGGTTATATTTGCCAAGCTAAATCCGGGTATAAGATATGTTCAAGGGATGAACGAAATCTTGGCACCACTGTTCTATGTCTTAAAAAATGATCCCAATGAGGAAGATGCGGCCTTTGCTGAAGCAGACACCTTTTTTTGTTTTGTTGAATTATTGAGTGGCTTCCGCGACCATTTCTGTCAGCAACTCGATAATAGTGTTGTTGGAATTCGTTCCACGATAACAAGATTGTCACAGCTTTTAAAGGAACATGATGAAGAGCTATGGCGTCATCTCGAGATCACAACCAAAGTCAATCCCCAATTTTATGCATTTAGATGGATCACCCTCCTTCTAACGCAGGAATTCAATTTCGCAGACAGCCTTCACATTTGGGATACACTTTTAAGTGACCCCGAAGGTCCTCAGGAAACCCTTCTTCGAGTATGCTGTGCAATGTTGATTCTCATTCGGAGGCGCTTACTAGCAGGTGATTTTACCTCAAATCTCAAACTACTGCAGAGCTATCCTCCGACCAACATTAGCCATCTGCTCTACGTTGCCAACAAGTTGCGGGTACAATCCTCAGGCTAA

Protein Analysis

465

Amino Acids

52.56

Weight (kDa)

5.68

Isoelectric Point (pI)

58.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RabGAP-TBC PF00566 236 - 428 2.2e-39 Rab-GTPase-TBC domain
RabGap-TBC_2 PF23436 269 - 441 3.2e-09 RabGAP TBC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1280
Acc36I ACCTGC 1 cut(s) 1280
AccB1I GGYRCC 1 cut(s) 886
AccB7I CCANNNNNTGG 2 cut(s) 441, 687
AccBSI CCGCTC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 488
AccII CGCG 3 cut(s) 102, 381, 990
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 7 cut(s) 129, 132, 200, 306, 932, 988, 1375
AclWI GGATC 3 cut(s) 288, 908, 1151
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 4 cut(s) 150, 393, 1029, 1166
AcuI CTGAAG 2 cut(s) 537, 963
AcyI GRCGYC 1 cut(s) 1090
AfaI GTAC 3 cut(s) 518, 748, 1380
AfeI AGCGCT 1 cut(s) 110
AfiI CCNNNNNNNGG 3 cut(s) 441, 476, 687
AgsI TTSAA 5 cut(s) 156, 814, 866, 971, 1171
AjnI CCWGG 2 cut(s) 230, 822
AjuI GAANNNNNNNTTGG 2 cut(s) 866, 898
AlwI GGATC 3 cut(s) 288, 908, 1151
AlwNI CAGNNNCTG 2 cut(s) 176, 687
Ama87I CYCGRG 2 cut(s) 251, 1097
Aor13HI TCCGGA 1 cut(s) 277
Aor51HI AGCGCT 1 cut(s) 110
AoxI GGCC 3 cut(s) 136, 348, 933
ApoI RAATTY 4 cut(s) 150, 393, 1029, 1166
Asp700I GAANNNNTTC 3 cut(s) 695, 1033, 1236
AspLEI GCGC 4 cut(s) 104, 111, 381, 1281
AspS9I GGNCC 4 cut(s) 63, 137, 432, 1223
AsuC2I CCSGG 1 cut(s) 849
AsuHPI GGTGA 6 cut(s) 3, 34, 188, 379, 1138, 1304
AvaI CYCGRG 2 cut(s) 251, 1097
AvaII GGWCC 3 cut(s) 63, 432, 1223
AxyI CCTNAGG 2 cut(s) 1227, 1387
BanI GGYRCC 1 cut(s) 886
BanII GRGCYC 1 cut(s) 258
BauI CACGAG 1 cut(s) 562
BbsI GAAGAC 1 cut(s) 243
BccI CCATC 5 cut(s) 95, 410, 565, 1134, 1356
BciT130I CCWGG 2 cut(s) 232, 824
BciVI GTATCC 2 cut(s) 420, 1192
BcnI CCSGG 1 cut(s) 849
BfaI CTAG 1 cut(s) 1286
BfmI CTRYAG 1 cut(s) 1319
BfoI RGCGCY 2 cut(s) 112, 1282
BfuAI ACCTGC 1 cut(s) 1280
BfuI GTATCC 2 cut(s) 420, 1192
BisI GCNGC 2 cut(s) 132, 933
BlsI GCNGC 2 cut(s) 133, 934
Bme1390I CCNGG 3 cut(s) 232, 824, 849
Bme18I GGWCC 3 cut(s) 63, 432, 1223
BmeT110I CYCGRG 2 cut(s) 251, 1097
BmgT120I GGNCC 4 cut(s) 63, 137, 432, 1223
BmiI GGNNCC 2 cut(s) 139, 888
BmrFI CCNGG 3 cut(s) 232, 824, 849
BmsI GCATC 4 cut(s) 667, 778, 796, 919
BpiI GAAGAC 1 cut(s) 243
BplI GAGNNNNNCTC 2 cut(s) 1315, 1347
BpmI CTGGAG 1 cut(s) 313
BpuEI CTTGAG 1 cut(s) 768
BpuMI CCSGG 1 cut(s) 849
BsaHI GRCGYC 1 cut(s) 1090
BsaJI CCNNGG 2 cut(s) 189, 231
BsaWI WCCGGW 1 cut(s) 277
Bsc4I CCNNNNNNNGG 3 cut(s) 441, 476, 687
Bse118I RCCGGY 2 cut(s) 134, 181
Bse21I CCTNAGG 2 cut(s) 1227, 1387
Bse3DI GCAATG 1 cut(s) 1263
BseAI TCCGGA 1 cut(s) 277
BseBI CCWGG 2 cut(s) 232, 824
BseDI CCNNGG 2 cut(s) 189, 231
BseGI GGATG 6 cut(s) 604, 658, 751, 811, 819, 876
BseLI CCNNNNNNNGG 3 cut(s) 441, 476, 687
BseMI GCAATG 1 cut(s) 1263
BseMII CTCAG 6 cut(s) 168, 183, 353, 483, 786, 1241
BseRI GAGGAG 3 cut(s) 58, 207, 237
BseYI CCCAGC 2 cut(s) 105, 422
Bsh1236I CGCG 3 cut(s) 102, 381, 990
Bsh1285I CGRYCG 2 cut(s) 100, 733
BshFI GGCC 3 cut(s) 138, 350, 935
BshNI GGYRCC 1 cut(s) 886
BsiEI CGRYCG 2 cut(s) 100, 733
BsiHKCI CYCGRG 2 cut(s) 251, 1097
BsiSI CCGG 4 cut(s) 135, 182, 278, 848
BslFI GGGAC 3 cut(s) 20, 298, 791
BslI CCNNNNNNNGG 3 cut(s) 441, 476, 687
BsmFI GGGAC 3 cut(s) 20, 298, 791
BsnI GGCC 3 cut(s) 138, 350, 935
BsoBI CYCGRG 2 cut(s) 251, 1097
Bsp1286I GDGCHC 1 cut(s) 258
Bsp13I TCCGGA 1 cut(s) 277
Bsp143I GATC 8 cut(s) 97, 280, 472, 635, 714, 913, 1101, 1143
BspACI CCGC 7 cut(s) 129, 132, 200, 306, 932, 988, 1375
BspANI GGCC 3 cut(s) 138, 350, 935
BspCNI CTCAG 6 cut(s) 169, 182, 352, 484, 785, 1240
BspEI TCCGGA 1 cut(s) 277
BspFNI CGCG 3 cut(s) 102, 381, 990
BspLI GGNNCC 2 cut(s) 139, 888
BspMAI CTGCAG 1 cut(s) 1323
BspMI ACCTGC 1 cut(s) 1280
BspPI GGATC 3 cut(s) 288, 908, 1151
BspQI GCTCTTC 1 cut(s) 1074
BspT107I GGYRCC 1 cut(s) 886
BsrBI CCGCTC 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 1263
BsrFI RCCGGY 2 cut(s) 134, 181
BssAI RCCGGY 2 cut(s) 134, 181
BssECI CCNNGG 2 cut(s) 189, 231
BssMI GATC 8 cut(s) 97, 280, 472, 635, 714, 913, 1101, 1143
BssNI GRCGYC 1 cut(s) 1090
BssSI CACGAG 1 cut(s) 562
Bst2BI CACGAG 1 cut(s) 562
Bst2UI CCWGG 2 cut(s) 232, 824
Bst4CI ACNGT 5 cut(s) 193, 432, 439, 516, 894
Bst6I CTCTTC 1 cut(s) 1074
BstACI GRCGYC 1 cut(s) 1090
BstC8I GCNNGC 3 cut(s) 56, 136, 183
BstDEI CTNAG 7 cut(s) 169, 177, 339, 492, 772, 1227, 1387
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 6 cut(s) 604, 658, 751, 811, 819, 876
BstFNI CGCG 3 cut(s) 102, 381, 990
BstH2I RGCGCY 2 cut(s) 112, 1282
BstHHI GCGC 4 cut(s) 104, 111, 381, 1281
BstKTI GATC 8 cut(s) 100, 283, 475, 638, 717, 916, 1104, 1146
BstMBI GATC 8 cut(s) 97, 280, 472, 635, 714, 913, 1101, 1143
BstMCI CGRYCG 2 cut(s) 100, 733
BstMWI GCNNNNNNNGC 3 cut(s) 108, 385, 938
BstNI CCWGG 2 cut(s) 232, 824
BstNSI RCATGY 1 cut(s) 763
BstSCI CCNGG 3 cut(s) 230, 822, 847
BstSFI CTRYAG 1 cut(s) 1319
BstUI CGCG 3 cut(s) 102, 381, 990
BstV2I GAAGAC 1 cut(s) 243
Bsu36I CCTNAGG 2 cut(s) 1227, 1387
BsuI GTATCC 2 cut(s) 420, 1192
BsuRI GGCC 3 cut(s) 138, 350, 935
BtgI CCRYGG 1 cut(s) 189
BtgZI GCGATG 1 cut(s) 384
BtsCI GGATG 6 cut(s) 604, 658, 751, 811, 819, 876
BtsIMutI CAGTG 2 cut(s) 435, 890
BveI ACCTGC 1 cut(s) 1280
Cac8I GCNNGC 3 cut(s) 56, 136, 183
CaiI CAGNNNCTG 2 cut(s) 176, 687
CfoI GCGC 4 cut(s) 104, 111, 381, 1281
Cfr10I RCCGGY 2 cut(s) 134, 181
Cfr13I GGNCC 4 cut(s) 63, 137, 432, 1223
CseI GACGC 2 cut(s) 673, 1079
Csp6I GTAC 3 cut(s) 517, 747, 1379
CviAII CATG 3 cut(s) 650, 760, 1073
CviQI GTAC 3 cut(s) 517, 747, 1379
DdeI CTNAG 7 cut(s) 169, 177, 339, 492, 772, 1227, 1387
DpnI GATC 8 cut(s) 99, 282, 474, 637, 716, 915, 1103, 1145
DpnII GATC 8 cut(s) 97, 280, 472, 635, 714, 913, 1101, 1143
DraI TTTAAA 2 cut(s) 529, 1065
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 1 cut(s) 1074
EarI CTCTTC 1 cut(s) 1074
Eco24I GRGCYC 1 cut(s) 258
Eco32I GATATC 2 cut(s) 325, 461
Eco47I GGWCC 3 cut(s) 63, 432, 1223
Eco47III AGCGCT 1 cut(s) 110
Eco57I CTGAAG 2 cut(s) 537, 963
Eco81I CCTNAGG 2 cut(s) 1227, 1387
Eco88I CYCGRG 2 cut(s) 251, 1097
EcoO109I RGGNCCY 1 cut(s) 1223
EcoRI GAATTC 2 cut(s) 1029, 1166
EcoRII CCWGG 2 cut(s) 230, 822
EcoRV GATATC 2 cut(s) 325, 461
EcoT22I ATGCAT 2 cut(s) 765, 1135
EcoT38I GRGCYC 1 cut(s) 258
FaeI CATG 3 cut(s) 653, 763, 1076
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FaqI GGGAC 3 cut(s) 20, 298, 791
FatI CATG 3 cut(s) 649, 759, 1072
FauI CCCGC 2 cut(s) 207, 1368
FblI GTMKAC 1 cut(s) 488
Fnu4HI GCNGC 2 cut(s) 132, 933
FokI GGATG 6 cut(s) 611, 645, 738, 806, 818, 883
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 2 cut(s) 132, 933
FspBI CTAG 1 cut(s) 1286
GlaI GCGC 4 cut(s) 103, 110, 380, 1280
GluI GCNGC 2 cut(s) 132, 933
GsaI CCCAGC 2 cut(s) 109, 426
GsuI CTGGAG 1 cut(s) 313
HaeII RGCGCY 2 cut(s) 112, 1282
HaeIII GGCC 3 cut(s) 138, 350, 935
HapII CCGG 4 cut(s) 135, 182, 278, 848
HgaI GACGC 2 cut(s) 673, 1079
HhaI GCGC 4 cut(s) 104, 111, 381, 1281
Hin1I GRCGYC 1 cut(s) 1090
Hin1II CATG 3 cut(s) 653, 763, 1076
Hin6I GCGC 4 cut(s) 102, 109, 379, 1279
HinP1I GCGC 4 cut(s) 102, 109, 379, 1279
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 8 cut(s) 33, 173, 269, 547, 667, 691, 779, 1264
HpaII CCGG 4 cut(s) 135, 182, 278, 848
HphI GGTGA 6 cut(s) 3, 34, 188, 379, 1138, 1304
Hpy166II GTNNAC 3 cut(s) 196, 435, 489
Hpy188I TCNGA 6 cut(s) 172, 178, 556, 640, 1275, 1335
Hpy8I GTNNAC 3 cut(s) 196, 435, 489
Hpy99I CGWCG 2 cut(s) 95, 98
HpyAV CCTTC 8 cut(s) 87, 358, 561, 808, 1163, 1196, 1214, 1247
HpyCH4III ACNGT 5 cut(s) 193, 432, 439, 516, 894
HpyCH4IV ACGT 1 cut(s) 1359
HpyCH4V TGCA 5 cut(s) 388, 763, 1133, 1256, 1321
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 385, 938
HpyF3I CTNAG 7 cut(s) 169, 177, 339, 492, 772, 1227, 1387
HpySE526I ACGT 1 cut(s) 1359
Hsp92I GRCGYC 1 cut(s) 1090
Hsp92II CATG 3 cut(s) 653, 763, 1076
HspAI GCGC 4 cut(s) 102, 109, 379, 1279
Kpn2I TCCGGA 1 cut(s) 277
KroI GCCGGC 2 cut(s) 134, 181
KroNI GCCGGC 2 cut(s) 136, 183
Kzo9I GATC 8 cut(s) 97, 280, 472, 635, 714, 913, 1101, 1143
LguI GCTCTTC 1 cut(s) 1074
LmnI GCTCC 3 cut(s) 220, 313, 655
LweI GCATC 4 cut(s) 667, 778, 796, 919
MaeI CTAG 1 cut(s) 1286
MaeII ACGT 1 cut(s) 1359
MaeIII GTNAC 4 cut(s) 510, 590, 1053, 1211
MalI GATC 8 cut(s) 99, 282, 474, 637, 716, 915, 1103, 1145
MbiI CCGCTC 1 cut(s) 308
MboI GATC 8 cut(s) 97, 280, 472, 635, 714, 913, 1101, 1143
MhlI GDGCHC 1 cut(s) 258
MlyI GAGTC 4 cut(s) 42, 167, 676, 773
MmeI TCCRAC 2 cut(s) 1006, 1358
Mph1103I ATGCAT 2 cut(s) 765, 1135
MroI TCCGGA 1 cut(s) 277
MroNI GCCGGC 2 cut(s) 134, 181
MroXI GAANNNNTTC 3 cut(s) 695, 1033, 1236
MseI TTAA 5 cut(s) 528, 605, 905, 1064, 1208
MslI CAYNNNNRTG 1 cut(s) 1138
MspI CCGG 4 cut(s) 135, 182, 278, 848
MspR9I CCNGG 3 cut(s) 232, 824, 849
MvaI CCWGG 2 cut(s) 232, 824
MvnI CGCG 3 cut(s) 102, 381, 990
MwoI GCNNNNNNNGC 3 cut(s) 108, 385, 938
NaeI GCCGGC 2 cut(s) 136, 183
NciI CCSGG 1 cut(s) 849
NdeII GATC 8 cut(s) 97, 280, 472, 635, 714, 913, 1101, 1143
NgoMIV GCCGGC 2 cut(s) 134, 181
NlaIII CATG 3 cut(s) 653, 763, 1076
NlaIV GGNNCC 2 cut(s) 139, 888
NmeAIII GCCGAG 1 cut(s) 376
NmuCI GTSAC 4 cut(s) 510, 590, 1053, 1211
NsiI ATGCAT 2 cut(s) 765, 1135
NspI RCATGY 1 cut(s) 763
PaeR7I CTCGAG 1 cut(s) 1097
PaqCI CACCTGC 1 cut(s) 1280
PciSI GCTCTTC 1 cut(s) 1074
PdiI GCCGGC 2 cut(s) 136, 183
PdmI GAANNNNTTC 3 cut(s) 695, 1033, 1236
PfeI GAWTC 4 cut(s) 269, 547, 691, 1264
PflMI CCANNNNNTGG 2 cut(s) 441, 687
PkrI GCNGC 2 cut(s) 133, 934
Ple19I CGATCG 1 cut(s) 100
PleI GAGTC 4 cut(s) 41, 167, 675, 773
PpsI GAGTC 4 cut(s) 41, 167, 675, 773
PpuMI RGGWCCY 1 cut(s) 1223
Psp5II RGGWCCY 1 cut(s) 1223
Psp6I CCWGG 2 cut(s) 230, 822
PspFI CCCAGC 2 cut(s) 105, 422
PspGI CCWGG 2 cut(s) 230, 822
PspN4I GGNNCC 2 cut(s) 139, 888
PspPI GGNCC 4 cut(s) 63, 137, 432, 1223
PspPPI RGGWCCY 1 cut(s) 1223
PstI CTGCAG 1 cut(s) 1323
PstNI CAGNNNCTG 2 cut(s) 176, 687
PvuI CGATCG 1 cut(s) 100
RsaI GTAC 3 cut(s) 518, 748, 1380
RsaNI GTAC 3 cut(s) 517, 747, 1379
RseI CAYNNNNRTG 1 cut(s) 1138
SalI GTCGAC 1 cut(s) 487
SapI GCTCTTC 1 cut(s) 1074
SaqAI TTAA 5 cut(s) 528, 605, 905, 1064, 1208
SatI GCNGC 2 cut(s) 132, 933
Sau3AI GATC 8 cut(s) 97, 280, 472, 635, 714, 913, 1101, 1143
Sau96I GGNCC 4 cut(s) 63, 137, 432, 1223
SchI GAGTC 4 cut(s) 42, 167, 676, 773
ScrFI CCNGG 3 cut(s) 232, 824, 849
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 4 cut(s) 667, 778, 796, 919
SfcI CTRYAG 1 cut(s) 1319
Sfr274I CTCGAG 1 cut(s) 1097
SinI GGWCC 3 cut(s) 63, 432, 1223
SlaI CTCGAG 1 cut(s) 1097
SmiMI CAYNNNNRTG 1 cut(s) 1138
SmlI CTYRAG 2 cut(s) 783, 1097
SmoI CTYRAG 2 cut(s) 783, 1097
SsiI CCGC 7 cut(s) 129, 132, 200, 306, 932, 988, 1375
SspMI CTAG 1 cut(s) 1286
StyD4I CCNGG 3 cut(s) 230, 822, 847
TaaI ACNGT 5 cut(s) 193, 432, 439, 516, 894
TaiI ACGT 1 cut(s) 1362
TaqI TCGA 7 cut(s) 90, 359, 488, 729, 1011, 1098, 1243
TaqII GACCGA 1 cut(s) 747
TatI WGTACW 1 cut(s) 516
TauI GCSGC 2 cut(s) 134, 935
TfiI GAWTC 4 cut(s) 269, 547, 691, 1264
Tru1I TTAA 5 cut(s) 528, 605, 905, 1064, 1208
Tru9I TTAA 5 cut(s) 528, 605, 905, 1064, 1208
TscAI CASTG 2 cut(s) 442, 897
TseFI GTSAC 4 cut(s) 510, 590, 1053, 1211
Tsp45I GTSAC 4 cut(s) 510, 590, 1053, 1211
TspDTI ATGAA 6 cut(s) 261, 387, 560, 615, 887, 1092
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 2 cut(s) 442, 897
Van91I CCANNNNNTGG 2 cut(s) 441, 687
VpaK11BI GGWCC 3 cut(s) 63, 432, 1223
XapI RAATTY 4 cut(s) 150, 393, 1029, 1166
XceI RCATGY 1 cut(s) 763
XhoI CTCGAG 1 cut(s) 1097
XmiI GTMKAC 1 cut(s) 488
XmnI GAANNNNTTC 3 cut(s) 695, 1033, 1236
XspI CTAG 1 cut(s) 1286
Zsp2I ATGCAT 2 cut(s) 765, 1135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.