Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001227

Physical Location & Seq

Genomic Coordinates Reverse (-)

1393968 .. 1403811

Gene Structure 9844 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1012400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1566 bp

ATGCGAAGACCGGGGCGGCAGCACTCGAAGCAAGCTGCCGGAGGAGGAGGAGGGGTGATGAAGGGCACGCTTGGGAGGTTATTGATCGGCGTCGTGGTTCTCCTGATCTGCACCGTTTCGTTTCTGGTTATGACCACGACCAGCGGCGGCGGCGGTGGTGGTGGATCTGGATTTGGCTCCGAGATTAACTTGGATAAACTTTGGAGAAGTGCTGATTCTGGTGGTTGGAGACCATCATCAGCTCCACGATCTGATTGGCCCCCTCCACCAAGTGAGACTAATGGCTACCTACGCGTGCGCTGTAATGGCGGTCTCAATCAACAACGTACAGCGATATGCAATGCTGTTCTCGCAGCAAGAATCATGAATGCTACACTTGTGCTGCCAGAACTGGATGCAAACTCTTTTTGGCATGATGACAGTGGTTTCGGCGGTATCTATGATGTTGAGCATTTTATCCAGTCATTGAGGTATGATGTAAAAATAGTACAAAAAATACCAGAAATTCGCAAAAATGGGAAGACCAAGAAGATAAAAGCTGTACAGCTTCGTCCCCCTCGAGATGCTCCTATCAGTTGGTATACTACAGATGCACTTGCGAAAATGAAGGAGCATGGTGCTATTTATCTTACTCCCTTTTCACATCGCTTGGCAGAAGAAATTGACAACCCTGAGTATCAACGGTTAAGGTGCAGGGTTAACTATCATGCTCTGAGGTTCAAGACAAATATAATGAAGATAAGTCAATCAATAGTAGATAAGCTTCGCTCACGAGGTCACTTCATGTCCATACATCTTCGTTTTGAGATGGATATGCTGGCATTTGCTGGCTGCTTTGATATTTTCAACCCCAAAGAGCAAGAGATATTGAAGAAGTATCGAAAAGAAAATTTTGCGCCAAAGAAGCTCGTCTACGAGGAAAGAAGAGCCATTGGAAAGTGCCCATTAACTCCTGAAGAGGTTGGCCTTATTTTGCGTGCAATGAGGTTTAACAATTCCACCAGGATATACCTAGCTGCTGGTGAACTATTTGGCGGGGAGAGATTCATGAAACCGTTTCGGGCTTTGTTCACTCACCTTGAGACCCACAGTTCTGTGGATCCCACAGACGAGCTATCAGAAAACACCCAGGGATTGTTAGGGTCTGCAGTGGACTACATGGTTTGTCTCCTCTCTGATATTTTTATGCCTACATATGACGGCCCTAGCAACTTTGCCAACAATCTCCTTGGACACCGCCTCTATTATGGCTTCCGGACCACACTTAGACCCGACAGGAAAGCTCTTGCTCCAGTCTTCATTGATCGAGAGAAGGGACGGACAGCTGGTTTTGAAGAAGCCGTAAGGAAAGCCATGCTCAAGACTAACCTTGGCGGGCCCCACAAGCGGATCCCCCCTGAGTCTTTCTATACAAATTCTTGGCCGGAATGTTTCTGCCAAACATCTCCCAAGAATCCAGAAGATAAATGTCCACCGGATAATGTTTTGGAAATTCTGGAGGGCCAGTTGAGTGAAGCAGCGGATGGCCGGGAATCAAATTCAACAATATCTGTAGCCGAAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0012505 GO:0031410 GO:0031982 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

O-FucT

Protein Analysis

522

Amino Acids

58.34

Weight (kDa)

9.24

Isoelectric Point (pI)

45.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
O-FucT	PF10250	96 - 416	4.3e-92	GDP-fucose protein O-fucosyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001227)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04910
fragaria_vesca	FvH4_4g13740 FvH4_4g13740 FvH4_4g13740 FvH4_4g13740
malus_domestica	MD04G1012400.v1.1 MD13G1283600.v1.1
prunus_persica	Prupe.1G158500_v2.0.a1 Prupe.1G158500_v2.0.a1 Prupe.1G158500_v2.0.a1 Prupe.1G158500_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1 Prupe.5G028000_v2.0.a1
pyrus_communis	pycom04g00990 pycom420g01220
rosa_chinensis	RchiOBHm_Chr4g0414391 RchiOBHm_Chr4g0414481
rosa_laevigata	RLG00000008150 RLG00000013051 RLG00000013052
rosa_multiflora	Rmu_sc0016990.1_g000002 Rmu_sc0029306.1_g000002 Rmu_sc0037565.1_g000001
rosa_roxburghii	Rroxscaffold_5G00358250 Rroxscaffold_5G00358290 Rroxscaffold_5G00358300 Rroxscaffold_7G00187950
rosa_rugosa	Rorug04G0127900
rosa_samantha	Rh4AG185800 Rh4BG185100 Rh4CG198400 Rh4DG182900 Rh4DG184100 Rh6AG244800 Rh6AG244900 Rh6CG249000
rosa_wichuraiana	Rw4G015890 Rw4G015920 Rw4G015940 Rw6G021200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	581, 912
AccII	CGCG	1 cut(s)	294
AccIII	TCCGGA	1 cut(s)	1254
AclWI	GGATC	5 cut(s)	172, 1094, 1107, 1384, 1397
AcoI	YGGCCR	2 cut(s)	1421, 1525
AcsI	RAATTY	5 cut(s)	504, 889, 1414, 1491, 1537
AcuI	CTGAAG	1 cut(s)	975
AcyI	GRCGYC	1 cut(s)	90
AdeI	CACNNNGTG	1 cut(s)	272
AfaI	GTAC	3 cut(s)	328, 489, 543
AfiI	CCNNNNNNNGG	1 cut(s)	1386
AflIII	ACRYGT	1 cut(s)	292
AgsI	TTSAA	5 cut(s)	721, 847, 871, 1334, 1542
AjnI	CCWGG	2 cut(s)	1001, 1128
Alw26I	GTCTC	5 cut(s)	223, 269, 317, 1076, 1172
AlwI	GGATC	5 cut(s)	172, 1094, 1107, 1384, 1397
Ama87I	CYCGRG	1 cut(s)	558
Aor13HI	TCCGGA	1 cut(s)	1254
AoxI	GGCC	7 cut(s)	257, 964, 1201, 1376, 1421, 1501, 1525
ApaI	GGGCCC	1 cut(s)	1380
ApeKI	GCWGC	7 cut(s)	19, 35, 353, 382, 831, 1016, 1517
ApoI	RAATTY	5 cut(s)	504, 889, 1414, 1491, 1537
ArsI	GACNNNNNNTTYG	2 cut(s)	410, 442
AspLEI	GCGC	2 cut(s)	300, 898
AspS9I	GGNCC	6 cut(s)	258, 1202, 1257, 1376, 1377, 1501
AsuC2I	CCSGG	2 cut(s)	12, 1529
AsuHPI	GGTGA	3 cut(s)	67, 1034, 1067
AvaI	CYCGRG	1 cut(s)	558
AvaII	GGWCC	1 cut(s)	1257
BaeGI	GKGCMC	3 cut(s)	68, 944, 1380
BamHI	GGATCC	2 cut(s)	1099, 1389
BanII	GRGCYC	1 cut(s)	1380
BarI	GAAGNNNNNNTAC	4 cut(s)	747, 779, 896, 928
BauI	CACGAG	1 cut(s)	771
BbsI	GAAGAC	3 cut(s)	13, 527, 1288
BbvI	GCAGC	7 cut(s)	22, 31, 365, 369, 818, 1003, 1529
BccI	CCATC	3 cut(s)	241, 802, 1517
BceAI	ACGGC	2 cut(s)	1216, 1325
BciT130I	CCWGG	2 cut(s)	1003, 1130
BcnI	CCSGG	2 cut(s)	12, 1529
BcoDI	GTCTC	5 cut(s)	223, 269, 317, 1076, 1172
BfaI	CTAG	2 cut(s)	1013, 1206
BfmI	CTRYAG	3 cut(s)	585, 1146, 1551
Bme1390I	CCNGG	4 cut(s)	12, 1003, 1130, 1529
Bme18I	GGWCC	1 cut(s)	1257
BmeT110I	CYCGRG	1 cut(s)	558
BmgT120I	GGNCC	6 cut(s)	258, 1202, 1257, 1376, 1377, 1501
BmiI	GGNNCC	6 cut(s)	178, 260, 1101, 1378, 1379, 1391
BmrFI	CCNGG	4 cut(s)	12, 1003, 1130, 1529
BmsI	GCATC	3 cut(s)	385, 553, 580
BpiI	GAAGAC	3 cut(s)	13, 527, 1288
BpmI	CTGGAG	2 cut(s)	1275, 1517
BpuEI	CTTGAG	2 cut(s)	1100, 1343
BpuMI	CCSGG	2 cut(s)	12, 1529
BsaHI	GRCGYC	1 cut(s)	90
BsaI	GGTCTC	3 cut(s)	223, 317, 1076
BsaJI	CCNNGG	5 cut(s)	11, 1128, 1129, 1228, 1369
BsaWI	WCCGGW	2 cut(s)	1254, 1474
BsaXI	ACNNNNNCTCC	4 cut(s)	39, 69, 1490, 1520
Bsc4I	CCNNNNNNNGG	1 cut(s)	1386
Bse1I	ACTGG	4 cut(s)	396, 460, 1292, 1504
Bse3DI	GCAATG	2 cut(s)	346, 987
BseAI	TCCGGA	1 cut(s)	1254
BseBI	CCWGG	2 cut(s)	1003, 1130
BseDI	CCNNGG	5 cut(s)	11, 1128, 1129, 1228, 1369
BseGI	GGATG	2 cut(s)	400, 1528
BseLI	CCNNNNNNNGG	1 cut(s)	1386
BseMI	GCAATG	2 cut(s)	346, 987
BseMII	CTCAG	3 cut(s)	663, 704, 1389
BseNI	ACTGG	4 cut(s)	396, 460, 1292, 1504
BseRI	GAGGAG	4 cut(s)	57, 60, 63, 1160
BseSI	GKGCMC	3 cut(s)	68, 944, 1380
BseXI	GCAGC	7 cut(s)	22, 31, 365, 369, 818, 1003, 1529
BsgI	GTGCAG	2 cut(s)	94, 712
Bsh1236I	CGCG	1 cut(s)	294
BshFI	GGCC	7 cut(s)	259, 966, 1203, 1378, 1423, 1503, 1527
BsiHKCI	CYCGRG	1 cut(s)	558
BsiSI	CCGG	6 cut(s)	11, 39, 1255, 1424, 1475, 1528
BslFI	GGGAC	2 cut(s)	537, 1329
BslI	CCNNNNNNNGG	1 cut(s)	1386
BsmAI	GTCTC	5 cut(s)	223, 269, 317, 1076, 1172
BsmFI	GGGAC	2 cut(s)	537, 1329
BsmI	GAATGC	1 cut(s)	373
BsnI	GGCC	7 cut(s)	259, 966, 1203, 1378, 1423, 1503, 1527
Bso31I	GGTCTC	3 cut(s)	223, 317, 1076
BsoBI	CYCGRG	1 cut(s)	558
Bsp120I	GGGCCC	1 cut(s)	1376
Bsp1286I	GDGCHC	3 cut(s)	68, 944, 1380
Bsp13I	TCCGGA	1 cut(s)	1254
Bsp1407I	TGTACA	1 cut(s)	541
Bsp143I	GATC	7 cut(s)	84, 105, 164, 248, 1099, 1303, 1389
BspANI	GGCC	7 cut(s)	259, 966, 1203, 1378, 1423, 1503, 1527
BspCNI	CTCAG	3 cut(s)	664, 705, 1390
BspEI	TCCGGA	1 cut(s)	1254
BspFNI	CGCG	1 cut(s)	294
BspHI	TCATGA	2 cut(s)	363, 1047
BspLI	GGNNCC	6 cut(s)	178, 260, 1101, 1378, 1379, 1391
BspMAI	CTGCAG	1 cut(s)	1150
BspPI	GGATC	5 cut(s)	172, 1094, 1107, 1384, 1397
BspQI	GCTCTTC	1 cut(s)	919
BspTNI	GGTCTC	3 cut(s)	223, 317, 1076
BsrDI	GCAATG	2 cut(s)	346, 987
BsrGI	TGTACA	1 cut(s)	541
BsrI	ACTGG	4 cut(s)	396, 460, 1292, 1504
BssECI	CCNNGG	5 cut(s)	11, 1128, 1129, 1228, 1369
BssMI	GATC	7 cut(s)	84, 105, 164, 248, 1099, 1303, 1389
BssNAI	GTATAC	1 cut(s)	582
BssNI	GRCGYC	1 cut(s)	90
BssSI	CACGAG	1 cut(s)	771
BssT1I	CCWWGG	2 cut(s)	1228, 1369
Bst1107I	GTATAC	1 cut(s)	582
Bst2BI	CACGAG	1 cut(s)	771
Bst2UI	CCWGG	2 cut(s)	1003, 1130
Bst4CI	ACNGT	5 cut(s)	115, 422, 684, 1056, 1091
Bst6I	CTCTTC	2 cut(s)	919, 951
BstACI	GRCGYC	1 cut(s)	90
BstAUI	TGTACA	1 cut(s)	541
BstC8I	GCNNGC	7 cut(s)	33, 68, 296, 819, 829, 978, 1376
BstDEI	CTNAG	4 cut(s)	672, 713, 1265, 1398
BstF5I	GGATG	2 cut(s)	400, 1528
BstFNI	CGCG	1 cut(s)	294
BstHHI	GCGC	2 cut(s)	300, 898
BstKTI	GATC	7 cut(s)	87, 108, 167, 251, 1102, 1306, 1392
BstMAI	GTCTC	5 cut(s)	223, 269, 317, 1076, 1172
BstMBI	GATC	7 cut(s)	84, 105, 164, 248, 1099, 1303, 1389
BstMWI	GCNNNNNNNGC	7 cut(s)	28, 150, 291, 306, 350, 904, 1384
BstNI	CCWGG	2 cut(s)	1003, 1130
BstSCI	CCNGG	4 cut(s)	10, 1001, 1128, 1527
BstSFI	CTRYAG	3 cut(s)	585, 1146, 1551
BstSLI	GKGCMC	3 cut(s)	68, 944, 1380
BstUI	CGCG	1 cut(s)	294
BstV1I	GCAGC	7 cut(s)	22, 31, 365, 369, 818, 1003, 1529
BstV2I	GAAGAC	3 cut(s)	13, 527, 1288
BstX2I	RGATCY	3 cut(s)	164, 1099, 1389
BstYI	RGATCY	3 cut(s)	164, 1099, 1389
BstZ17I	GTATAC	1 cut(s)	582
BsuRI	GGCC	7 cut(s)	259, 966, 1203, 1378, 1423, 1503, 1527
BtgZI	GCGATG	1 cut(s)	629
BtsCI	GGATG	2 cut(s)	400, 1528
BtsI	GCAGTG	1 cut(s)	1155
BtsIMutI	CAGTG	2 cut(s)	427, 1155
Cac8I	GCNNGC	7 cut(s)	33, 68, 296, 819, 829, 978, 1376
CciI	TCATGA	2 cut(s)	363, 1047
CfoI	GCGC	2 cut(s)	300, 898
Cfr13I	GGNCC	6 cut(s)	258, 1202, 1257, 1376, 1377, 1501
CseI	GACGC	1 cut(s)	79
Csp6I	GTAC	3 cut(s)	327, 488, 542
CviAII	CATG	8 cut(s)	364, 413, 614, 707, 784, 1048, 1159, 1354
CviQI	GTAC	3 cut(s)	327, 488, 542
DdeI	CTNAG	4 cut(s)	672, 713, 1265, 1398
DpnI	GATC	7 cut(s)	86, 107, 166, 250, 1101, 1305, 1391
DpnII	GATC	7 cut(s)	84, 105, 164, 248, 1099, 1303, 1389
DraIII	CACNNNGTG	1 cut(s)	272
EaeI	YGGCCR	2 cut(s)	1421, 1525
Eam1104I	CTCTTC	2 cut(s)	919, 951
EarI	CTCTTC	2 cut(s)	919, 951
Eco130I	CCWWGG	2 cut(s)	1228, 1369
Eco24I	GRGCYC	1 cut(s)	1380
Eco31I	GGTCTC	3 cut(s)	223, 317, 1076
Eco47I	GGWCC	1 cut(s)	1257
Eco57I	CTGAAG	1 cut(s)	975
Eco88I	CYCGRG	1 cut(s)	558
EcoO109I	RGGNCCY	1 cut(s)	1377
EcoRII	CCWGG	2 cut(s)	1001, 1128
EcoT14I	CCWWGG	2 cut(s)	1228, 1369
EcoT38I	GRGCYC	1 cut(s)	1380
ErhI	CCWWGG	2 cut(s)	1228, 1369
FaeI	CATG	8 cut(s)	367, 416, 617, 710, 787, 1051, 1162, 1357
FaqI	GGGAC	2 cut(s)	537, 1329
FatI	CATG	8 cut(s)	363, 412, 613, 706, 783, 1047, 1158, 1353
FauI	CCCGC	2 cut(s)	1028, 1367
FauNDI	CATATG	1 cut(s)	1195
FblI	GTMKAC	2 cut(s)	581, 912
FokI	GGATG	2 cut(s)	407, 1535
FriOI	GRGCYC	1 cut(s)	1380
FspBI	CTAG	2 cut(s)	1013, 1206
GlaI	GCGC	2 cut(s)	299, 897
GsuI	CTGGAG	2 cut(s)	1275, 1517
HaeIII	GGCC	7 cut(s)	259, 966, 1203, 1378, 1423, 1503, 1527
HapII	CCGG	6 cut(s)	11, 39, 1255, 1424, 1475, 1528
HgaI	GACGC	1 cut(s)	79
HhaI	GCGC	2 cut(s)	300, 898
Hin1I	GRCGYC	1 cut(s)	90
Hin1II	CATG	8 cut(s)	367, 416, 617, 710, 787, 1051, 1162, 1357
Hin6I	GCGC	2 cut(s)	298, 896
HinP1I	GCGC	2 cut(s)	298, 896
HincII	GTYRAC	1 cut(s)	700
HindII	GTYRAC	1 cut(s)	700
HindIII	AAGCTT	1 cut(s)	761
HinfI	GANTC	6 cut(s)	215, 360, 1044, 1400, 1453, 1532
HpaI	GTTAAC	1 cut(s)	700
HpaII	CCGG	6 cut(s)	11, 39, 1255, 1424, 1475, 1528
HphI	GGTGA	3 cut(s)	67, 1034, 1067
Hpy166II	GTNNAC	7 cut(s)	582, 700, 913, 1025, 1071, 1153, 1472
Hpy188I	TCNGA	5 cut(s)	181, 253, 714, 1120, 1177
Hpy8I	GTNNAC	7 cut(s)	582, 700, 913, 1025, 1071, 1153, 1472
Hpy99I	CGWCG	1 cut(s)	95
HpyAV	CCTTC	3 cut(s)	55, 601, 1306
HpyCH4III	ACNGT	5 cut(s)	115, 422, 684, 1056, 1091
HpyCH4IV	ACGT	1 cut(s)	325
HpyCH4V	TGCA	7 cut(s)	111, 339, 398, 593, 693, 980, 1148
HpyF10VI	GCNNNNNNNGC	7 cut(s)	28, 150, 291, 306, 350, 904, 1384
HpyF3I	CTNAG	4 cut(s)	672, 713, 1265, 1398
HpySE526I	ACGT	1 cut(s)	325
Hsp92I	GRCGYC	1 cut(s)	90
Hsp92II	CATG	8 cut(s)	367, 416, 617, 710, 787, 1051, 1162, 1357
HspAI	GCGC	2 cut(s)	298, 896
Kpn2I	TCCGGA	1 cut(s)	1254
KspAI	GTTAAC	1 cut(s)	700
Kzo9I	GATC	7 cut(s)	84, 105, 164, 248, 1099, 1303, 1389
LguI	GCTCTTC	1 cut(s)	919
LmnI	GCTCC	5 cut(s)	182, 247, 571, 610, 1294
Lsp1109I	GCAGC	7 cut(s)	22, 31, 365, 369, 818, 1003, 1529
LweI	GCATC	3 cut(s)	385, 553, 580
MaeI	CTAG	2 cut(s)	1013, 1206
MaeII	ACGT	1 cut(s)	325
MaeIII	GTNAC	1 cut(s)	776
MalI	GATC	7 cut(s)	86, 107, 166, 250, 1101, 1305, 1391
MboI	GATC	7 cut(s)	84, 105, 164, 248, 1099, 1303, 1389
MflI	RGATCY	3 cut(s)	164, 1099, 1389
MhlI	GDGCHC	3 cut(s)	68, 944, 1380
MluCI	AATT	7 cut(s)	504, 660, 889, 994, 1414, 1491, 1537
MluI	ACGCGT	1 cut(s)	292
MlyI	GAGTC	1 cut(s)	1409
MmeI	TCCRAC	1 cut(s)	206
MroI	TCCGGA	1 cut(s)	1254
MseI	TTAA	5 cut(s)	186, 686, 699, 947, 990
MspA1I	CMGCKG	3 cut(s)	144, 1325, 1520
MspI	CCGG	6 cut(s)	11, 39, 1255, 1424, 1475, 1528
MspR9I	CCNGG	4 cut(s)	12, 1003, 1130, 1529
Mva1269I	GAATGC	1 cut(s)	373
MvaI	CCWGG	2 cut(s)	1003, 1130
MvnI	CGCG	1 cut(s)	294
MwoI	GCNNNNNNNGC	7 cut(s)	28, 150, 291, 306, 350, 904, 1384
NciI	CCSGG	2 cut(s)	12, 1529
NdeI	CATATG	1 cut(s)	1195
NdeII	GATC	7 cut(s)	84, 105, 164, 248, 1099, 1303, 1389
NlaIII	CATG	8 cut(s)	367, 416, 617, 710, 787, 1051, 1162, 1357
NlaIV	GGNNCC	6 cut(s)	178, 260, 1101, 1378, 1379, 1391
NmuCI	GTSAC	1 cut(s)	776
PaeR7I	CTCGAG	1 cut(s)	558
PagI	TCATGA	2 cut(s)	363, 1047
PasI	CCCWGGG	1 cut(s)	1129
PciSI	GCTCTTC	1 cut(s)	919
PcsI	WCGNNNNNNNCGW	1 cut(s)	556
PctI	GAATGC	1 cut(s)	373
PfeI	GAWTC	5 cut(s)	215, 360, 1044, 1453, 1532
PleI	GAGTC	1 cut(s)	1408
PpsI	GAGTC	1 cut(s)	1408
Psp6I	CCWGG	2 cut(s)	1001, 1128
PspGI	CCWGG	2 cut(s)	1001, 1128
PspN4I	GGNNCC	6 cut(s)	178, 260, 1101, 1378, 1379, 1391
PspOMI	GGGCCC	1 cut(s)	1376
PspPI	GGNCC	6 cut(s)	258, 1202, 1257, 1376, 1377, 1501
PstI	CTGCAG	1 cut(s)	1150
PsuI	RGATCY	3 cut(s)	164, 1099, 1389
PvuII	CAGCTG	1 cut(s)	1325
RsaI	GTAC	3 cut(s)	328, 489, 543
RsaNI	GTAC	3 cut(s)	327, 488, 542
SapI	GCTCTTC	1 cut(s)	919
SaqAI	TTAA	5 cut(s)	186, 686, 699, 947, 990
Sau3AI	GATC	7 cut(s)	84, 105, 164, 248, 1099, 1303, 1389
Sau96I	GGNCC	6 cut(s)	258, 1202, 1257, 1376, 1377, 1501
SchI	GAGTC	1 cut(s)	1409
ScrFI	CCNGG	4 cut(s)	12, 1003, 1130, 1529
SduI	GDGCHC	3 cut(s)	68, 944, 1380
SfaNI	GCATC	3 cut(s)	385, 553, 580
SfcI	CTRYAG	3 cut(s)	585, 1146, 1551
Sfr274I	CTCGAG	1 cut(s)	558
SinI	GGWCC	1 cut(s)	1257
SlaI	CTCGAG	1 cut(s)	558
SmlI	CTYRAG	3 cut(s)	558, 1079, 1358
SmoI	CTYRAG	3 cut(s)	558, 1079, 1358
Sse9I	AATT	7 cut(s)	504, 660, 889, 994, 1414, 1491, 1537
SspMI	CTAG	2 cut(s)	1013, 1206
StyD4I	CCNGG	4 cut(s)	10, 1001, 1128, 1527
StyI	CCWWGG	2 cut(s)	1228, 1369
TaaI	ACNGT	5 cut(s)	115, 422, 684, 1056, 1091
TaiI	ACGT	1 cut(s)	328
TaqI	TCGA	4 cut(s)	26, 559, 880, 1306
TasI	AATT	7 cut(s)	504, 660, 889, 994, 1414, 1491, 1537
TatI	WGTACW	2 cut(s)	487, 541
TauI	GCSGC	4 cut(s)	19, 147, 150, 153
TfiI	GAWTC	5 cut(s)	215, 360, 1044, 1453, 1532
Tru1I	TTAA	5 cut(s)	186, 686, 699, 947, 990
Tru9I	TTAA	5 cut(s)	186, 686, 699, 947, 990
TscAI	CASTG	2 cut(s)	427, 1155
TseFI	GTSAC	1 cut(s)	776
TseI	GCWGC	7 cut(s)	19, 35, 353, 382, 831, 1016, 1517
Tsp45I	GTSAC	1 cut(s)	776
TspDTI	ATGAA	8 cut(s)	74, 380, 620, 749, 772, 1036, 1064, 1288
TspGWI	ACGGA	1 cut(s)	1333
TspRI	CASTG	2 cut(s)	427, 1155
VpaK11BI	GGWCC	1 cut(s)	1257
XapI	RAATTY	5 cut(s)	504, 889, 1414, 1491, 1537
XcmI	CCANNNNNNNNNTGG	1 cut(s)	252
XhoI	CTCGAG	1 cut(s)	558
XmiI	GTMKAC	2 cut(s)	581, 912
XspI	CTAG	2 cut(s)	1013, 1206

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.