Prupe.1G158500_v2.0.a1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001227
Physical Location & Seq
Reverse (-)
12560979 .. 12566290
5312 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G158500.2

Sequence Viewer

Length: 1554 bp
ATGCGAAGACCGGGGCTTTATAATCGGCAGCATGCGAAGCAAGCAGGAGCGGTGAAGGGCATGATTGGGAGGCTATTGATCGCTGTTCTGGTTGTCATCTGCACCATTTCGTTCCTGGTTTTGTGGACGAGCAATGGCGGTGGTCCATCCGGCTTTCGCCCTGAGTTGAACTTGGATAAACTTTGGAGAAGTGCTGATTCTGGTGGTTGGAGACCATCATCAGCTCCACGATCTAATTGGCCTCCTCCACCTAGGGAGACTAACGGCTACCTACGAGTGCGCTGTAATGGTGGTCTCAATCAACAACGTGCAGCGATATGTAATGCTGTTCTTGCAGCAAGAATCATGAATGCTACACTTGTGCTGCCTGAATTGGATGCAAACTCTTTTTGGCATGATGACAGTGGTTTCCGTGGTATCTATGATGTTGAGCATTTTATCATGTCATTGAGGTATGATGTAAAAATTGTAGAAAAAATACCAGAAATTCGCAAAAATGGGAAGACAAAGAAGATAAAAGCTGTACAGCTTCGTCCTCCTAGAGATGCTCCTATCAGTTGGTATACTACGGATGCTCTTGCGAAAATGAAGGAGCATGGCGCTATTTATCTTACTCCCTTTTCACATCGTTTGGCAGAAGAAATTGACAACCCTGAGTATCAACGGTTGAGGTGCAGAGTGAACTATCATGCTCTGAGATTTAAGACAAATATAATGAAGCTAAGTCAGTCAATAGTTGATAAGCTTCGCTCACAAGGTCACTTCATGTCCATACATCTTCGTTTTGAAATGGATATGCTGGCATTTGCTGGCTGTTTTGATATTTTCAACCTCAAAGAGCAAGAGATTTTGAAGAAATATCGGAAAGAAAATTTTGCACCAAAGAAGCTTGTGTATGAGGAAAGAAGAGCCATTGGAAAATGCCCATTAACTCCAGAAGAGGTTGGTCATATTTTACGTGCAATGGGGTTTAACAATTCTACCAGGATATACCTAGCTGCTGGTGAACTATTTGGTGGAGAGAGATTCATGAAACCGTTCCGGGTTTTGTTCCCTCACCTTGAGAACCACAGTTCTGTAGATCCCGCAGACGAGCTAGCAGAAAATACCCAGGGATTGTTAGGGTCTGCGGTGGACTACATGGTTTGCCTCCTCTCTGATATTTTTATGCCTACATATGATGGCCCTAGCAACTTTGCCAACAATCTCCTTGGACACCGCCTATATTATGGCTTCCGGACCACAATTAGACCCGATAGGAAAGCTCTTGCTCCAATCTTCGTTGATCTAGAGAAGGGACGGACAACTGGTTTTGAAGAAGCTGTTAGGCAAGCCATGCTCAATACTAACCTTGGCGGGCCCCACAAGCGGATTCCACCTGATTCTTTCTATACAAATTCTTGGCCCGAATGTTTCTGCCAAACATCTCCAAAGAATCCAGCACATAAATGTCCACCAGATAATGTTTTGGAAATTCTTAACAGCCAGTTGGGTGAAGCAACTGATGACCTGGAATCCGTGCCCGAATCAAATTCAACAATATCTGTGGCATAA

Protein Analysis

518

Amino Acids

58.48

Weight (kDa)

9.13

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 21
AccBSI CCGCTC 1 cut(s) 50
AccI GTMKAC 1 cut(s) 563
AccIII TCCGGA 1 cut(s) 1236
AciI CCGC 7 cut(s) 50, 138, 1086, 1130, 1219, 1356, 1369
AclWI GGATC 1 cut(s) 1076
AcsI RAATTY 5 cut(s) 486, 871, 1396, 1473, 1531
AfaI GTAC 1 cut(s) 525
AfiI CCNNNNNNNGG 1 cut(s) 1368
AgsI TTSAA 6 cut(s) 169, 788, 829, 853, 1316, 1536
AjnI CCWGG 4 cut(s) 114, 983, 1110, 1509
Alw26I GTCTC 3 cut(s) 205, 251, 299
AlwI GGATC 1 cut(s) 1076
Aor13HI TCCGGA 1 cut(s) 1236
AoxI GGCC 4 cut(s) 239, 1183, 1358, 1403
ApaI GGGCCC 1 cut(s) 1362
ApeKI GCWGC 5 cut(s) 28, 311, 335, 364, 998
ApoI RAATTY 5 cut(s) 486, 871, 1396, 1473, 1531
Asp700I GAANNNNTTC 1 cut(s) 1037
AspA2I CCTAGG 1 cut(s) 251
AspLEI GCGC 2 cut(s) 282, 602
AspS9I GGNCC 6 cut(s) 143, 1184, 1239, 1358, 1359, 1404
AsuC2I CCSGG 2 cut(s) 12, 1043
AsuHPI GGTGA 4 cut(s) 64, 1016, 1049, 1505
AsuNHI GCTAGC 1 cut(s) 1096
AvaII GGWCC 2 cut(s) 143, 1239
AvrII CCTAGG 1 cut(s) 251
BaeGI GKGCMC 2 cut(s) 1362, 1524
BanII GRGCYC 1 cut(s) 1362
BbsI GAAGAC 2 cut(s) 13, 509
BbvI GCAGC 5 cut(s) 40, 323, 347, 351, 985
BccI CCATC 3 cut(s) 154, 223, 1175
BceAI ACGGC 1 cut(s) 280
BciT130I CCWGG 4 cut(s) 116, 985, 1112, 1511
BcnI CCSGG 2 cut(s) 12, 1043
BcoDI GTCTC 3 cut(s) 205, 251, 299
BfaI CTAG 6 cut(s) 252, 540, 995, 1097, 1188, 1289
BfmI CTRYAG 1 cut(s) 1077
BfoI RGCGCY 1 cut(s) 603
BisI GCNGC 5 cut(s) 29, 312, 336, 365, 999
BlnI CCTAGG 1 cut(s) 251
BlsI GCNGC 5 cut(s) 30, 313, 337, 366, 1000
Bme1390I CCNGG 6 cut(s) 12, 116, 985, 1043, 1112, 1511
Bme18I GGWCC 2 cut(s) 143, 1239
BmgT120I GGNCC 6 cut(s) 143, 1184, 1239, 1358, 1359, 1404
BmiI GGNNCC 2 cut(s) 1360, 1361
BmrFI CCNGG 6 cut(s) 12, 116, 985, 1043, 1112, 1511
BmsI GCATC 3 cut(s) 367, 535, 562
BmtI GCTAGC 1 cut(s) 1100
BpiI GAAGAC 2 cut(s) 13, 509
BpmI CTGGAG 1 cut(s) 918
BpuEI CTTGAG 1 cut(s) 1082
BpuMI CCSGG 2 cut(s) 12, 1043
BsaAI YACGTR 1 cut(s) 959
BsaI GGTCTC 2 cut(s) 205, 299
BsaJI CCNNGG 7 cut(s) 11, 251, 412, 1110, 1111, 1210, 1351
BsaWI WCCGGW 1 cut(s) 1236
Bsc4I CCNNNNNNNGG 1 cut(s) 1368
Bse1I ACTGG 2 cut(s) 1312, 1486
Bse3DI GCAATG 2 cut(s) 139, 969
BseAI TCCGGA 1 cut(s) 1236
BseBI CCWGG 4 cut(s) 116, 985, 1112, 1511
BseDI CCNNGG 7 cut(s) 11, 251, 412, 1110, 1111, 1210, 1351
BseGI GGATG 3 cut(s) 146, 382, 577
BseLI CCNNNNNNNGG 1 cut(s) 1368
BseMI GCAATG 2 cut(s) 139, 969
BseMII CTCAG 3 cut(s) 153, 645, 686
BseNI ACTGG 2 cut(s) 1312, 1486
BseRI GAGGAG 2 cut(s) 234, 1142
BseSI GKGCMC 2 cut(s) 1362, 1524
BseXI GCAGC 5 cut(s) 40, 323, 347, 351, 985
BsgI GTGCAG 3 cut(s) 85, 330, 694
BshFI GGCC 4 cut(s) 241, 1185, 1360, 1405
BsiSI CCGG 4 cut(s) 11, 150, 1042, 1237
BslFI GGGAC 1 cut(s) 1311
BslI CCNNNNNNNGG 1 cut(s) 1368
BsmAI GTCTC 3 cut(s) 205, 251, 299
BsmFI GGGAC 1 cut(s) 1311
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 4 cut(s) 241, 1185, 1360, 1405
Bso31I GGTCTC 2 cut(s) 205, 299
Bsp120I GGGCCC 1 cut(s) 1358
Bsp1286I GDGCHC 2 cut(s) 1362, 1524
Bsp13I TCCGGA 1 cut(s) 1236
Bsp1407I TGTACA 1 cut(s) 523
Bsp143I GATC 4 cut(s) 78, 230, 1081, 1285
BspACI CCGC 7 cut(s) 50, 138, 1086, 1130, 1219, 1356, 1369
BspANI GGCC 4 cut(s) 241, 1185, 1360, 1405
BspCNI CTCAG 3 cut(s) 154, 646, 687
BspEI TCCGGA 1 cut(s) 1236
BspHI TCATGA 2 cut(s) 345, 1029
BspLI GGNNCC 2 cut(s) 1360, 1361
BspOI GCTAGC 1 cut(s) 1100
BspPI GGATC 1 cut(s) 1076
BspQI GCTCTTC 1 cut(s) 901
BspTNI GGTCTC 2 cut(s) 205, 299
BsrBI CCGCTC 1 cut(s) 50
BsrDI GCAATG 2 cut(s) 139, 969
BsrGI TGTACA 1 cut(s) 523
BsrI ACTGG 2 cut(s) 1312, 1486
BssECI CCNNGG 7 cut(s) 11, 251, 412, 1110, 1111, 1210, 1351
BssMI GATC 4 cut(s) 78, 230, 1081, 1285
BssNAI GTATAC 1 cut(s) 564
BssT1I CCWWGG 3 cut(s) 251, 1210, 1351
Bst1107I GTATAC 1 cut(s) 564
Bst2UI CCWGG 4 cut(s) 116, 985, 1112, 1511
Bst4CI ACNGT 4 cut(s) 404, 666, 1038, 1073
Bst6I CTCTTC 2 cut(s) 901, 933
BstAPI GCANNNNNTGC 1 cut(s) 1336
BstAUI TGTACA 1 cut(s) 523
BstBAI YACGTR 1 cut(s) 959
BstC8I GCNNGC 7 cut(s) 33, 42, 801, 811, 1098, 1332, 1358
BstDEI CTNAG 4 cut(s) 162, 654, 695, 722
BstDSI CCRYGG 1 cut(s) 412
BstF5I GGATG 3 cut(s) 146, 382, 577
BstH2I RGCGCY 1 cut(s) 603
BstHHI GCGC 2 cut(s) 282, 602
BstKTI GATC 4 cut(s) 81, 233, 1084, 1288
BstMAI GTCTC 3 cut(s) 205, 251, 299
BstMBI GATC 4 cut(s) 78, 230, 1081, 1285
BstMWI GCNNNNNNNGC 5 cut(s) 37, 41, 332, 1336, 1366
BstNI CCWGG 4 cut(s) 116, 985, 1112, 1511
BstNSI RCATGY 1 cut(s) 35
BstSCI CCNGG 6 cut(s) 10, 114, 983, 1041, 1110, 1509
BstSFI CTRYAG 1 cut(s) 1077
BstSLI GKGCMC 2 cut(s) 1362, 1524
BstV1I GCAGC 5 cut(s) 40, 323, 347, 351, 985
BstV2I GAAGAC 2 cut(s) 13, 509
BstX2I RGATCY 1 cut(s) 1081
BstYI RGATCY 1 cut(s) 1081
BstZ17I GTATAC 1 cut(s) 564
BsuRI GGCC 4 cut(s) 241, 1185, 1360, 1405
BtgI CCRYGG 1 cut(s) 412
BtsCI GGATG 3 cut(s) 146, 382, 577
BtsIMutI CAGTG 1 cut(s) 409
Cac8I GCNNGC 7 cut(s) 33, 42, 801, 811, 1098, 1332, 1358
CciI TCATGA 2 cut(s) 345, 1029
CfoI GCGC 2 cut(s) 282, 602
Cfr13I GGNCC 6 cut(s) 143, 1184, 1239, 1358, 1359, 1404
Csp6I GTAC 1 cut(s) 524
CspCI CAANNNNNGTGG 3 cut(s) 121, 156, 1527
CviQI GTAC 1 cut(s) 524
DdeI CTNAG 4 cut(s) 162, 654, 695, 722
DpnI GATC 4 cut(s) 80, 232, 1083, 1287
DpnII GATC 4 cut(s) 78, 230, 1081, 1285
Eam1104I CTCTTC 2 cut(s) 901, 933
EarI CTCTTC 2 cut(s) 901, 933
Eco130I CCWWGG 3 cut(s) 251, 1210, 1351
Eco24I GRGCYC 1 cut(s) 1362
Eco31I GGTCTC 2 cut(s) 205, 299
Eco47I GGWCC 2 cut(s) 143, 1239
EcoO109I RGGNCCY 1 cut(s) 1359
EcoRII CCWGG 4 cut(s) 114, 983, 1110, 1509
EcoT14I CCWWGG 3 cut(s) 251, 1210, 1351
EcoT38I GRGCYC 1 cut(s) 1362
ErhI CCWWGG 3 cut(s) 251, 1210, 1351
FaqI GGGAC 1 cut(s) 1311
FauI CCCGC 2 cut(s) 1093, 1349
FauNDI CATATG 1 cut(s) 1177
FblI GTMKAC 1 cut(s) 563
Fnu4HI GCNGC 5 cut(s) 29, 312, 336, 365, 999
FokI GGATG 3 cut(s) 133, 389, 584
FriOI GRGCYC 1 cut(s) 1362
Fsp4HI GCNGC 5 cut(s) 29, 312, 336, 365, 999
FspBI CTAG 6 cut(s) 252, 540, 995, 1097, 1188, 1289
GlaI GCGC 2 cut(s) 281, 601
GluI GCNGC 5 cut(s) 29, 312, 336, 365, 999
GsuI CTGGAG 1 cut(s) 918
HaeII RGCGCY 1 cut(s) 603
HaeIII GGCC 4 cut(s) 241, 1185, 1360, 1405
HapII CCGG 4 cut(s) 11, 150, 1042, 1237
HhaI GCGC 2 cut(s) 282, 602
Hin6I GCGC 2 cut(s) 280, 600
HinP1I GCGC 2 cut(s) 280, 600
HindIII AAGCTT 2 cut(s) 743, 887
HinfI GANTC 8 cut(s) 197, 342, 1026, 1372, 1382, 1435, 1514, 1526
HpaII CCGG 4 cut(s) 11, 150, 1042, 1237
HphI GGTGA 4 cut(s) 64, 1016, 1049, 1505
Hpy166II GTNNAC 6 cut(s) 126, 564, 682, 1007, 1135, 1454
Hpy188I TCNGA 3 cut(s) 696, 864, 1159
Hpy188III TCNNGA 5 cut(s) 346, 935, 1030, 1237, 1289
Hpy8I GTNNAC 6 cut(s) 126, 564, 682, 1007, 1135, 1454
HpyAV CCTTC 3 cut(s) 49, 583, 1288
HpyCH4III ACNGT 4 cut(s) 404, 666, 1038, 1073
HpyCH4IV ACGT 2 cut(s) 307, 958
HpyCH4V TGCA 7 cut(s) 102, 311, 335, 380, 675, 878, 962
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 41, 332, 1336, 1366
HpyF3I CTNAG 4 cut(s) 162, 654, 695, 722
HpySE526I ACGT 2 cut(s) 307, 958
HspAI GCGC 2 cut(s) 280, 600
Kpn2I TCCGGA 1 cut(s) 1236
Kzo9I GATC 4 cut(s) 78, 230, 1081, 1285
LguI GCTCTTC 1 cut(s) 901
LmnI GCTCC 5 cut(s) 47, 229, 553, 592, 1276
Lsp1109I GCAGC 5 cut(s) 40, 323, 347, 351, 985
LweI GCATC 3 cut(s) 367, 535, 562
MaeI CTAG 6 cut(s) 252, 540, 995, 1097, 1188, 1289
MaeII ACGT 2 cut(s) 307, 958
MaeIII GTNAC 1 cut(s) 758
MalI GATC 4 cut(s) 80, 232, 1083, 1287
MbiI CCGCTC 1 cut(s) 50
MboI GATC 4 cut(s) 78, 230, 1081, 1285
MflI RGATCY 1 cut(s) 1081
MhlI GDGCHC 2 cut(s) 1362, 1524
MmeI TCCRAC 1 cut(s) 188
MroI TCCGGA 1 cut(s) 1236
MroXI GAANNNNTTC 1 cut(s) 1037
MseI TTAA 4 cut(s) 702, 929, 972, 1479
MslI CAYNNNNRTG 1 cut(s) 1447
MspI CCGG 4 cut(s) 11, 150, 1042, 1237
MspR9I CCNGG 6 cut(s) 12, 116, 985, 1043, 1112, 1511
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 4 cut(s) 116, 985, 1112, 1511
MwoI GCNNNNNNNGC 5 cut(s) 37, 41, 332, 1336, 1366
NciI CCSGG 2 cut(s) 12, 1043
NdeI CATATG 1 cut(s) 1177
NdeII GATC 4 cut(s) 78, 230, 1081, 1285
NheI GCTAGC 1 cut(s) 1096
NlaIV GGNNCC 2 cut(s) 1360, 1361
NmuCI GTSAC 1 cut(s) 758
NspI RCATGY 1 cut(s) 35
PaeI GCATGC 1 cut(s) 35
PagI TCATGA 2 cut(s) 345, 1029
PasI CCCWGGG 1 cut(s) 1111
PciSI GCTCTTC 1 cut(s) 901
PctI GAATGC 1 cut(s) 355
PdmI GAANNNNTTC 1 cut(s) 1037
PfeI GAWTC 8 cut(s) 197, 342, 1026, 1372, 1382, 1435, 1514, 1526
PkrI GCNGC 5 cut(s) 30, 313, 337, 366, 1000
Ppu21I YACGTR 1 cut(s) 959
PsiI TTATAA 1 cut(s) 21
Psp6I CCWGG 4 cut(s) 114, 983, 1110, 1509
PspGI CCWGG 4 cut(s) 114, 983, 1110, 1509
PspN4I GGNNCC 2 cut(s) 1360, 1361
PspOMI GGGCCC 1 cut(s) 1358
PspPI GGNCC 6 cut(s) 143, 1184, 1239, 1358, 1359, 1404
PsuI RGATCY 1 cut(s) 1081
RsaI GTAC 1 cut(s) 525
RsaNI GTAC 1 cut(s) 524
RseI CAYNNNNRTG 1 cut(s) 1447
SapI GCTCTTC 1 cut(s) 901
SaqAI TTAA 4 cut(s) 702, 929, 972, 1479
SatI GCNGC 5 cut(s) 29, 312, 336, 365, 999
Sau3AI GATC 4 cut(s) 78, 230, 1081, 1285
Sau96I GGNCC 6 cut(s) 143, 1184, 1239, 1358, 1359, 1404
ScrFI CCNGG 6 cut(s) 12, 116, 985, 1043, 1112, 1511
SduI GDGCHC 2 cut(s) 1362, 1524
SfaNI GCATC 3 cut(s) 367, 535, 562
SfcI CTRYAG 1 cut(s) 1077
SinI GGWCC 2 cut(s) 143, 1239
SmiMI CAYNNNNRTG 1 cut(s) 1447
SmlI CTYRAG 1 cut(s) 1061
SmoI CTYRAG 1 cut(s) 1061
SphI GCATGC 1 cut(s) 35
SsiI CCGC 7 cut(s) 50, 138, 1086, 1130, 1219, 1356, 1369
SspMI CTAG 6 cut(s) 252, 540, 995, 1097, 1188, 1289
StyD4I CCNGG 6 cut(s) 10, 114, 983, 1041, 1110, 1509
StyI CCWWGG 3 cut(s) 251, 1210, 1351
TaaI ACNGT 4 cut(s) 404, 666, 1038, 1073
TaiI ACGT 2 cut(s) 310, 961
TatI WGTACW 1 cut(s) 523
TfiI GAWTC 8 cut(s) 197, 342, 1026, 1372, 1382, 1435, 1514, 1526
Tru1I TTAA 4 cut(s) 702, 929, 972, 1479
Tru9I TTAA 4 cut(s) 702, 929, 972, 1479
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 1 cut(s) 758
TseI GCWGC 5 cut(s) 28, 311, 335, 364, 998
Tsp45I GTSAC 1 cut(s) 758
TspDTI ATGAA 6 cut(s) 362, 602, 731, 754, 1018, 1046
TspGWI ACGGA 4 cut(s) 401, 584, 1315, 1507
TspRI CASTG 1 cut(s) 409
VpaK11BI GGWCC 2 cut(s) 143, 1239
XapI RAATTY 5 cut(s) 486, 871, 1396, 1473, 1531
XbaI TCTAGA 1 cut(s) 1288
XceI RCATGY 1 cut(s) 35
XcmI CCANNNNNNNNNTGG 2 cut(s) 112, 234
XmaJI CCTAGG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 563
XmnI GAANNNNTTC 1 cut(s) 1037
XspI CTAG 6 cut(s) 252, 540, 995, 1097, 1188, 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.