RchiOBHm_Chr4g0414481

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001227
Physical Location & Seq
Forward (+)
39021744 .. 39026214
4471 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38488

Sequence Viewer

Length: 1416 bp
ATGAACATTTTTCATGCCTGGAACCCTAATGCGGTGATCAATTTGGAAAAACTTAGGAGAAGTGCTGACTCTCGAGGTTGGAGGCCGTCATCAGCTCCACGATCGGATTGGCCTCCTCCACCAAGCGAGACTAACGGCTACCTACGTGTGCGCTGTAATGGTGGTCTCAATCAACAACGTACTGCGATCTGCAATGCAGTTCTGGCAGCAAGAATAATGAATGCTACCCTTGTGCTGCCGGAATTGGATGCAAACTCATTTTGGCATGATGACAGTGGTTTTCAGGGAATCTATGATGTTGAGCATTTCATCAAGTCATTAAGGTTTGATGTAAATATTGTAGAAAGGATGCCCGAAATTCGCAAAAATGGGAAGACCAAGAAGATAAAAGCTGTACAGCTCCGACCACCAAGAGATGCTCCTATCAGTTGGTATACTACAGATGCTCTTCAGAAAATGAAGGAGCATGGGGCTATCTATCTAACTCCCTTTTCACATCGTTTGGCGGAAGAAATTGACAACGCCGAGTACCAACGGCTGAGGTGCAGGGTTAACTATCATGCTCTGATATTTAAGCCAAATATTATGAAGTTAAGTCAATCAATAGTTGATAAGCTCCGTACACAAGGTCACTTCATGTCTATACATCTTCGTTTTGAGATGGATATGCTGGCATTTGCTGGGTGCTTTGATATCTTTAACCCAAAAGAGCAAGAGATTTTGAAGAAGTATCGAAATGAAAATTTTGCACCAAAGAAGCTTGTTTATAAGGAAAGAAGAGCAATCGGAAAATGCCCATTAACTCCAGAAGAGGTCGGTCTTATTTTACGCGCAATGGAATTTAACAATTCTACCAGGATATACCTAGCTGCTGGTGAACTATTTGGTGGGGAGAGATTCATGAAACCATTCCGTGCTTTGTTCCCTCACCTTGAGAACCACAGTTCTGTGGAGCACTCAGATGAGCTACCAGAGAACACCCAGGGATTAATAGGGTCTGCAGTGGATTACATGGTTTGTCTACTTTCTGATATTTTTATGCCTACATATGATGGACCTAGCAACTTTGCCAACAATCTCCTTGGACACCGCCTCTATTATGGTTTCCGGACCACAATTAGACCTGATAGAAAAGCCCTTGCTCCAATCTTCATTGATCGTGAAAAAGGACGAAAATCAGGTTTCGAAGAAGCTGTTAGGCAAGTGATGCTAAAAACAAACCTTGGCGGCCCTCACAAGCGGATCTCACCTGAGTCTTTCTATACAAATTCTTGGCCAGAATGCTTCTGCCAAACATCTACAAAGAATCTGGCAGATAAATGTCCACCAGATAATGTTTTGGAAATTCTGAACAGCCAGTTAAGTCAAGAGAACGATGATCCGGAATCAAATTCAACATCTGTTAGAGAGAGATGA

Protein Analysis

471

Amino Acids

54.12

Weight (kDa)

8.9

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 47 - 367 5.7e-92 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 768
AccI GTMKAC 2 cut(s) 434, 1021
AccII CGCG 1 cut(s) 831
AccIII TCCGGA 2 cut(s) 1107, 1381
AciI CCGC 5 cut(s) 32, 506, 1090, 1227, 1240
AclWI GGATC 2 cut(s) 1250, 1373
AcoI YGGCCR 1 cut(s) 1274
AcsI RAATTY 6 cut(s) 357, 742, 839, 1267, 1344, 1390
AcuI CTGAAG 1 cut(s) 434
AfaI GTAC 4 cut(s) 181, 396, 530, 622
AfiI CCNNNNNNNGG 1 cut(s) 31
AflIII ACRYGT 1 cut(s) 145
AgsI TTSAA 2 cut(s) 724, 1395
AjnI CCWGG 3 cut(s) 17, 854, 981
AluBI AGCT 8 cut(s) 95, 392, 400, 616, 760, 869, 967, 1193
AluI AGCT 8 cut(s) 95, 392, 400, 616, 760, 869, 967, 1193
Alw21I GWGCWC 1 cut(s) 957
Alw26I GTCTC 2 cut(s) 122, 170
AlwI GGATC 2 cut(s) 1250, 1373
Ama87I CYCGRG 1 cut(s) 72
Aor13HI TCCGGA 2 cut(s) 1107, 1381
AoxI GGCC 4 cut(s) 83, 110, 1228, 1274
ApeKI GCWGC 3 cut(s) 206, 235, 869
ApoI RAATTY 6 cut(s) 357, 742, 839, 1267, 1344, 1390
AseI ATTAAT 1 cut(s) 989
Asp700I GAANNNNTTC 1 cut(s) 908
AspLEI GCGC 2 cut(s) 153, 833
AspS9I GGNCC 3 cut(s) 1055, 1110, 1229
AsuHPI GGTGA 4 cut(s) 46, 887, 920, 1239
AsuII TTCGAA 1 cut(s) 1185
AvaI CYCGRG 1 cut(s) 72
AvaII GGWCC 2 cut(s) 1055, 1110
BalI TGGCCA 1 cut(s) 1276
BbsI GAAGAC 1 cut(s) 380
Bbv12I GWGCWC 1 cut(s) 957
BbvCI CCTCAGC 1 cut(s) 539
BbvI GCAGC 3 cut(s) 218, 222, 856
BccI CCATC 2 cut(s) 655, 1046
BceAI ACGGC 3 cut(s) 70, 151, 551
BciT130I CCWGG 3 cut(s) 19, 856, 983
BclI TGATCA 1 cut(s) 36
BcoDI GTCTC 2 cut(s) 122, 170
BfaI CTAG 2 cut(s) 866, 1059
BfmI CTRYAG 2 cut(s) 438, 999
BisI GCNGC 4 cut(s) 207, 236, 870, 1228
BlsI GCNGC 4 cut(s) 208, 237, 871, 1229
Bme1390I CCNGG 3 cut(s) 19, 856, 983
Bme18I GGWCC 2 cut(s) 1055, 1110
BmeT110I CYCGRG 1 cut(s) 72
BmgT120I GGNCC 3 cut(s) 1055, 1110, 1229
BmiI GGNNCC 1 cut(s) 23
BmrFI CCNGG 3 cut(s) 19, 856, 983
BmsI GCATC 5 cut(s) 238, 339, 406, 433, 1197
BpiI GAAGAC 1 cut(s) 380
BpmI CTGGAG 1 cut(s) 789
Bpu10I CCTNAGC 1 cut(s) 539
Bpu14I TTCGAA 1 cut(s) 1185
BpuEI CTTGAG 1 cut(s) 953
BsaAI YACGTR 1 cut(s) 146
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 4 cut(s) 981, 982, 1081, 1222
BsaWI WCCGGW 2 cut(s) 1107, 1381
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse1I ACTGG 1 cut(s) 1357
Bse3DI GCAATG 2 cut(s) 199, 840
BseAI TCCGGA 2 cut(s) 1107, 1381
BseBI CCWGG 3 cut(s) 19, 856, 983
BseDI CCNNGG 4 cut(s) 981, 982, 1081, 1222
BseGI GGATG 2 cut(s) 253, 354
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMI GCAATG 2 cut(s) 199, 840
BseMII CTCAG 3 cut(s) 530, 972, 1242
BseNI ACTGG 1 cut(s) 1357
BseRI GAGGAG 1 cut(s) 105
BseXI GCAGC 3 cut(s) 218, 222, 856
BseYI CCCAGC 1 cut(s) 680
BsgI GTGCAG 1 cut(s) 565
Bsh1236I CGCG 1 cut(s) 831
Bsh1285I CGRYCG 1 cut(s) 104
BshFI GGCC 4 cut(s) 85, 112, 1230, 1276
BsiEI CGRYCG 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 957
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 3 cut(s) 239, 1108, 1382
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 2 cut(s) 122, 170
BsmI GAATGC 2 cut(s) 226, 1286
BsnI GGCC 4 cut(s) 85, 112, 1230, 1276
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 72
Bsp119I TTCGAA 1 cut(s) 1185
Bsp1286I GDGCHC 1 cut(s) 957
Bsp13I TCCGGA 2 cut(s) 1107, 1381
Bsp1407I TGTACA 1 cut(s) 394
Bsp143I GATC 6 cut(s) 36, 101, 186, 1156, 1242, 1378
BspACI CCGC 5 cut(s) 32, 506, 1090, 1227, 1240
BspANI GGCC 4 cut(s) 85, 112, 1230, 1276
BspCNI CTCAG 3 cut(s) 531, 971, 1243
BspEI TCCGGA 2 cut(s) 1107, 1381
BspFNI CGCG 1 cut(s) 831
BspHI TCATGA 1 cut(s) 900
BspLI GGNNCC 1 cut(s) 23
BspMAI CTGCAG 1 cut(s) 1003
BspPI GGATC 2 cut(s) 1250, 1373
BspQI GCTCTTC 2 cut(s) 453, 772
BspT104I TTCGAA 1 cut(s) 1185
BspTNI GGTCTC 1 cut(s) 170
BsrDI GCAATG 2 cut(s) 199, 840
BsrGI TGTACA 1 cut(s) 394
BsrI ACTGG 1 cut(s) 1357
BssECI CCNNGG 4 cut(s) 981, 982, 1081, 1222
BssMI GATC 6 cut(s) 36, 101, 186, 1156, 1242, 1378
BssNAI GTATAC 1 cut(s) 435
BssT1I CCWWGG 2 cut(s) 1081, 1222
Bst1107I GTATAC 1 cut(s) 435
Bst2UI CCWGG 3 cut(s) 19, 856, 983
Bst4CI ACNGT 2 cut(s) 275, 944
Bst6I CTCTTC 3 cut(s) 453, 772, 804
BstAPI GCANNNNNTGC 1 cut(s) 1207
BstAUI TGTACA 1 cut(s) 394
BstBAI YACGTR 1 cut(s) 146
BstBI TTCGAA 1 cut(s) 1185
BstC8I GCNNGC 1 cut(s) 672
BstDEI CTNAG 4 cut(s) 53, 539, 958, 1251
BstF5I GGATG 2 cut(s) 253, 354
BstFNI CGCG 1 cut(s) 831
BstHHI GCGC 2 cut(s) 153, 833
BstKTI GATC 6 cut(s) 39, 104, 189, 1159, 1245, 1381
BstMAI GTCTC 2 cut(s) 122, 170
BstMBI GATC 6 cut(s) 36, 101, 186, 1156, 1242, 1378
BstMCI CGRYCG 1 cut(s) 104
BstMWI GCNNNNNNNGC 2 cut(s) 203, 1207
BstNI CCWGG 3 cut(s) 19, 856, 983
BstSCI CCNGG 3 cut(s) 17, 854, 981
BstSFI CTRYAG 2 cut(s) 438, 999
BstUI CGCG 1 cut(s) 831
BstV1I GCAGC 3 cut(s) 218, 222, 856
BstV2I GAAGAC 1 cut(s) 380
BstX2I RGATCY 1 cut(s) 1242
BstYI RGATCY 1 cut(s) 1242
BstZ17I GTATAC 1 cut(s) 435
BsuRI GGCC 4 cut(s) 85, 112, 1230, 1276
BtsCI GGATG 2 cut(s) 253, 354
BtsI GCAGTG 1 cut(s) 1008
BtsIMutI CAGTG 2 cut(s) 280, 1008
Cac8I GCNNGC 1 cut(s) 672
CciI TCATGA 1 cut(s) 900
CfoI GCGC 2 cut(s) 153, 833
Cfr13I GGNCC 3 cut(s) 1055, 1110, 1229
Csp6I GTAC 4 cut(s) 180, 395, 529, 621
CviAII CATG 7 cut(s) 14, 266, 467, 560, 637, 901, 1012
CviQI GTAC 4 cut(s) 180, 395, 529, 621
DdeI CTNAG 4 cut(s) 53, 539, 958, 1251
DpnI GATC 6 cut(s) 38, 103, 188, 1158, 1244, 1380
DpnII GATC 6 cut(s) 36, 101, 186, 1156, 1242, 1378
EaeI YGGCCR 1 cut(s) 1274
Eam1104I CTCTTC 3 cut(s) 453, 772, 804
EarI CTCTTC 3 cut(s) 453, 772, 804
EciI GGCGGA 1 cut(s) 521
Eco130I CCWWGG 2 cut(s) 1081, 1222
Eco31I GGTCTC 1 cut(s) 170
Eco32I GATATC 1 cut(s) 694
Eco47I GGWCC 2 cut(s) 1055, 1110
Eco57I CTGAAG 1 cut(s) 434
Eco88I CYCGRG 1 cut(s) 72
EcoRII CCWGG 3 cut(s) 17, 854, 981
EcoRV GATATC 1 cut(s) 694
EcoT14I CCWWGG 2 cut(s) 1081, 1222
ErhI CCWWGG 2 cut(s) 1081, 1222
FaeI CATG 7 cut(s) 17, 269, 470, 563, 640, 904, 1015
FatI CATG 7 cut(s) 13, 265, 466, 559, 636, 900, 1011
FauNDI CATATG 1 cut(s) 1048
FbaI TGATCA 1 cut(s) 36
FblI GTMKAC 2 cut(s) 434, 1021
Fnu4HI GCNGC 4 cut(s) 207, 236, 870, 1228
FokI GGATG 2 cut(s) 260, 361
Fsp4HI GCNGC 4 cut(s) 207, 236, 870, 1228
FspBI CTAG 2 cut(s) 866, 1059
GlaI GCGC 2 cut(s) 152, 832
GluI GCNGC 4 cut(s) 207, 236, 870, 1228
GsaI CCCAGC 1 cut(s) 684
GsuI CTGGAG 1 cut(s) 789
HaeIII GGCC 4 cut(s) 85, 112, 1230, 1276
HapII CCGG 3 cut(s) 239, 1108, 1382
HhaI GCGC 2 cut(s) 153, 833
Hin1II CATG 7 cut(s) 17, 269, 470, 563, 640, 904, 1015
Hin6I GCGC 2 cut(s) 151, 831
HinP1I GCGC 2 cut(s) 151, 831
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 6 cut(s) 68, 288, 897, 1253, 1306, 1385
HpaI GTTAAC 1 cut(s) 553
HpaII CCGG 3 cut(s) 239, 1108, 1382
HphI GGTGA 4 cut(s) 46, 887, 920, 1239
Hpy166II GTNNAC 6 cut(s) 435, 553, 623, 878, 1022, 1325
Hpy188I TCNGA 8 cut(s) 106, 404, 453, 567, 788, 961, 1030, 1350
Hpy188III TCNNGA 7 cut(s) 72, 806, 901, 1108, 1160, 1367, 1382
Hpy8I GTNNAC 6 cut(s) 435, 553, 623, 878, 1022, 1325
HpyAV CCTTC 1 cut(s) 454
HpyCH4III ACNGT 2 cut(s) 275, 944
HpyCH4IV ACGT 2 cut(s) 145, 178
HpyCH4V TGCA 6 cut(s) 192, 197, 251, 546, 749, 1001
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 1207
HpyF3I CTNAG 4 cut(s) 53, 539, 958, 1251
HpySE526I ACGT 2 cut(s) 145, 178
Hsp92II CATG 7 cut(s) 17, 269, 470, 563, 640, 904, 1015
HspAI GCGC 2 cut(s) 151, 831
Kpn2I TCCGGA 2 cut(s) 1107, 1381
Ksp22I TGATCA 1 cut(s) 36
KspAI GTTAAC 1 cut(s) 553
Kzo9I GATC 6 cut(s) 36, 101, 186, 1156, 1242, 1378
LguI GCTCTTC 2 cut(s) 453, 772
LmnI GCTCC 7 cut(s) 100, 405, 424, 463, 621, 952, 1147
Lsp1109I GCAGC 3 cut(s) 218, 222, 856
LweI GCATC 5 cut(s) 238, 339, 406, 433, 1197
MaeI CTAG 2 cut(s) 866, 1059
MaeII ACGT 2 cut(s) 145, 178
MaeIII GTNAC 1 cut(s) 629
MalI GATC 6 cut(s) 38, 103, 188, 1158, 1244, 1380
MboI GATC 6 cut(s) 36, 101, 186, 1156, 1242, 1378
MflI RGATCY 1 cut(s) 1242
MhlI GDGCHC 1 cut(s) 957
MlsI TGGCCA 1 cut(s) 1276
MluNI TGGCCA 1 cut(s) 1276
MlyI GAGTC 2 cut(s) 62, 1262
MmeI TCCRAC 2 cut(s) 59, 427
MnlI CCTC 9 cut(s) 68, 75, 123, 126, 534, 805, 936, 1103, 1242
Mox20I TGGCCA 1 cut(s) 1276
MroI TCCGGA 2 cut(s) 1107, 1381
MroXI GAANNNNTTC 1 cut(s) 908
MscI TGGCCA 1 cut(s) 1276
MseI TTAA 9 cut(s) 320, 552, 573, 593, 699, 800, 843, 989, 1361
MslI CAYNNNNRTG 1 cut(s) 960
Msp20I TGGCCA 1 cut(s) 1276
MspI CCGG 3 cut(s) 239, 1108, 1382
MspR9I CCNGG 3 cut(s) 19, 856, 983
Mva1269I GAATGC 2 cut(s) 226, 1286
MvaI CCWGG 3 cut(s) 19, 856, 983
MvnI CGCG 1 cut(s) 831
MwoI GCNNNNNNNGC 2 cut(s) 203, 1207
NdeI CATATG 1 cut(s) 1048
NdeII GATC 6 cut(s) 36, 101, 186, 1156, 1242, 1378
NlaIII CATG 7 cut(s) 17, 269, 470, 563, 640, 904, 1015
NlaIV GGNNCC 1 cut(s) 23
NmeAIII GCCGAG 1 cut(s) 550
NmuCI GTSAC 1 cut(s) 629
NspV TTCGAA 1 cut(s) 1185
PaeR7I CTCGAG 1 cut(s) 72
PagI TCATGA 1 cut(s) 900
PasI CCCWGGG 1 cut(s) 982
PciSI GCTCTTC 2 cut(s) 453, 772
PctI GAATGC 2 cut(s) 226, 1286
PdmI GAANNNNTTC 1 cut(s) 908
PfeI GAWTC 4 cut(s) 288, 897, 1306, 1385
PkrI GCNGC 4 cut(s) 208, 237, 871, 1229
Ple19I CGATCG 1 cut(s) 104
PleI GAGTC 2 cut(s) 62, 1261
PpsI GAGTC 2 cut(s) 62, 1261
Ppu21I YACGTR 1 cut(s) 146
PshBI ATTAAT 1 cut(s) 989
PsiI TTATAA 1 cut(s) 768
Psp6I CCWGG 3 cut(s) 17, 854, 981
PspFI CCCAGC 1 cut(s) 680
PspGI CCWGG 3 cut(s) 17, 854, 981
PspN4I GGNNCC 1 cut(s) 23
PspPI GGNCC 3 cut(s) 1055, 1110, 1229
PstI CTGCAG 1 cut(s) 1003
PsuI RGATCY 1 cut(s) 1242
PvuI CGATCG 1 cut(s) 104
RsaI GTAC 4 cut(s) 181, 396, 530, 622
RsaNI GTAC 4 cut(s) 180, 395, 529, 621
RseI CAYNNNNRTG 1 cut(s) 960
SapI GCTCTTC 2 cut(s) 453, 772
SaqAI TTAA 9 cut(s) 320, 552, 573, 593, 699, 800, 843, 989, 1361
SatI GCNGC 4 cut(s) 207, 236, 870, 1228
Sau3AI GATC 6 cut(s) 36, 101, 186, 1156, 1242, 1378
Sau96I GGNCC 3 cut(s) 1055, 1110, 1229
SchI GAGTC 2 cut(s) 62, 1262
ScrFI CCNGG 3 cut(s) 19, 856, 983
SduI GDGCHC 1 cut(s) 957
SfaNI GCATC 5 cut(s) 238, 339, 406, 433, 1197
SfcI CTRYAG 2 cut(s) 438, 999
Sfr274I CTCGAG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 1185
SinI GGWCC 2 cut(s) 1055, 1110
SlaI CTCGAG 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 960
SmlI CTYRAG 2 cut(s) 72, 932
SmoI CTYRAG 2 cut(s) 72, 932
SsiI CCGC 5 cut(s) 32, 506, 1090, 1227, 1240
SspI AATATT 2 cut(s) 337, 583
SspMI CTAG 2 cut(s) 866, 1059
StyD4I CCNGG 3 cut(s) 17, 854, 981
StyI CCWWGG 2 cut(s) 1081, 1222
TaaI ACNGT 2 cut(s) 275, 944
TaiI ACGT 2 cut(s) 148, 181
TaqI TCGA 3 cut(s) 73, 733, 1185
TaqII GACCGA 1 cut(s) 806
TatI WGTACW 1 cut(s) 394
TauI GCSGC 1 cut(s) 1230
TfiI GAWTC 4 cut(s) 288, 897, 1306, 1385
Tru1I TTAA 9 cut(s) 320, 552, 573, 593, 699, 800, 843, 989, 1361
Tru9I TTAA 9 cut(s) 320, 552, 573, 593, 699, 800, 843, 989, 1361
TscAI CASTG 2 cut(s) 280, 1008
TseFI GTSAC 1 cut(s) 629
TseI GCWGC 3 cut(s) 206, 235, 869
Tsp45I GTSAC 1 cut(s) 629
TspGWI ACGGA 2 cut(s) 608, 902
TspRI CASTG 2 cut(s) 280, 1008
VpaK11BI GGWCC 2 cut(s) 1055, 1110
VspI ATTAAT 1 cut(s) 989
XapI RAATTY 6 cut(s) 357, 742, 839, 1267, 1344, 1390
XcmI CCANNNNNNNNNTGG 1 cut(s) 105
XhoI CTCGAG 1 cut(s) 72
XmiI GTMKAC 2 cut(s) 434, 1021
XmnI GAANNNNTTC 1 cut(s) 908
XspI CTAG 2 cut(s) 866, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.