Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001017

Physical Location & Seq

Genomic Coordinates Reverse (-)

2157254 .. 2164774

Gene Structure 7521 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1018700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGAACCCAATTTCCGTAGTTTCTGCTTCTGCTTCTGCTTCTGTCATCCAAATGGCCACCTTTTTCTTAGCGCCAAACTACGTCCTGGGCAGCTCTTCTGATTGCGGGCACACCCTTCAGTTCCGTTCCAACAATAATTCCAGAAGCGGACAACTTTATCATAGAAAATGGCACGGAAGCCGAGGTGGGATTTCTGTCAGTCGTGGATTTAGAGTGTTATGCCAGTCCAAGACAGAAGAAAAGCAGATACGAAGGCATTCACCTTTCCTGGAACGTGTGTTACTAGATGATAATGATGCTTCAGAATCTGATAGGTGGAAAGCAGTTCCTGATATTTGGAGATCTTCAGCTGCGAAATATGGTGACCTAGTAGCATTGGTGGATCCCTATCATGACCCACCTTCGAGTATAACTTATAAACAGCTTGAGCAGGAAATTTTGGACTTTGCTGAAGGGATGAGAGTTGTTGGAGTAAAGCCAGACGAAAAAATTGCACTGTTTGCTGATAATTCATGCCGTTGGCTTGTTGCTGATCAAGGTATAATGGCCACTGGAGCAATCAATGTGGTGAGAGGTTCGAGGTCATCAGTCGAAGAGTTACTGCAAATATACAATCATTCTGATAGTGTTGCGCTTGCTGTGGACAGTCCTGAGTTGTTCAATCGGATATCAGAAGAATTTTGTTCCAAGGCGATGAAATTTATTGTTCTGCTATGGGGGGAGAAGTCAAGCCTGAAAAATGAAGGAAAGGTTCCTATTTTCGACTACAAGGAGATTCTAGATTTGGGACGAGAGAGTCGCAAGAGTTTGCTTGATTCTGATGATAAACAACAGTCTGATACGTATGAAGTTATTAAATCGGATGATGTTGCTACATTAGTATATACGAGTGGAACAAGTGGCAACCCAAAAGGTGTCATGCTCACACATAGGAATTTGTTGCATCAGATAAAGAACCTAGGGGATGCTGTACCTTGTGTAGGTGGCGACAAATTTCTTAGCATGCTTCCACCATGGCATTGCTATGAACGAGCTTGTGAGTATTTCGCTTTTACGTGTGGAGTTGAGCAATTTTACACCAGTGTGCGGAAAATGAAGGATGATTTGCGAAAATATCAGCCAACCTATATAATTTCAGTTCCTTTAGTGTATGAGTCACTTTACAGCGGTATTCAGAAACAAATTTCATCCGCGTCTACTGTTCGTAAGCTTATTGTGCTTACATTCATAAGGATCAGTATGGCGTACATGGAGTTTAAGAGGATCTATGAGGGAACGTATCTGACGAGGAACCAGAAGCAACCTTCGTATCTTGTTTCAGTAGCAGATTGGTTATGGGCGCGAATAATTGCTGCGTTATTATGGCCTTTGCATGTGCTGGGAAAGAAACTAGTCTACAGTAAAATTCAATCCGCCATTGGAATATCAAAGGCCGGCATCAGTGGAGGAGGTTGTCTGCCTTCACATGTTGATAAATTTTTCGAGGCAATTGACGTGAAACTGCAGAATGGATATGGTTTAACAGAGACTTCTCCTGTTGTTGCTTCTCGACGATCAAATTTTAATGTTATTGGTTCTGTTGGGATACCAATTCGACATACTGAGTTCAAAGTCGTAGATTCTGAAACCGGTGAAGTTCTTTCTCCTGGTTTGAGCGGAATTCTGAAAGTTAGGGGACCTCAAGTGATGAAGGGATACTACAAGAATCCAGGCGCTACAAAGAAAGCATTGGATGACGATGGGTGGCTAGACACTGGAGACATTGGTTGGATTGCTCCTCACCATTCAATAGGGCGAAGTCGTTGTTGTGGAGGGGTGGTTGTTCTTGAAGGACGTGCCAAGGACACTATCGTTCTTTTAACAGGGGAAAACGTTGAACCGGTAGAGATTGAGGAAGCTGCCTTGAGAAGTAGTCTAATTCAACAAATTGTTGTCATCGGCCAGGATCAGCGACGCCTTGGGGCAATTGTTGTTCCAAACAAAGAAGAGGCTTTACTCGCAGCCAAAAAGTTGGCTGCTGTAGATGCTGCTGATGCCTCCGGCCTAAGCAACGATAGATTGACAAGATTGGTTTATGAAGAGCTGAGAAAATGGACTTCAGGGTGTTCATTTCAAATCGGACCGATCATAATCGCGGACGAACCCTTTACGATTGACAGTGGCCTAATGACTCCAACCATGAAAATTCGACGGGACCGAGTCGTGGCTCAATACAAAGAACAAATAGACAACCTTTTCAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0008922 GO:0009058 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0015645 GO:0016053 GO:0016874 GO:0016877 GO:0016878 GO:0019752 GO:0030497 GO:0031967 GO:0031975 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00071 ko01100 ko01212 ko02024 ko03320 ko04146 ko04216 ko04714 ko04920 map00061 map00071 map01100 map01212 map02024 map03320 map04146 map04216 map04714 map04920

Pfam Domains

Protein Families

AMP-binding

Protein Analysis

748

Amino Acids

82.95

Weight (kDa)

8.26

Isoelectric Point (pI)

42.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	115 - 567	1.3e-74	AMP-binding enzyme
AMP-binding_C_3	PF23562	615 - 671	7.2e-10	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001017)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23790 AT4G14070
fragaria_vesca	FvH4_4g13140 FvH4_4g13151 FvH4_4g13151 FvH4_4g13151 FvH4_4g13151 FvH4_4g13151 FvH4_4g13151 FvH4_4g13151 FvH4_4g13151
malus_domestica	MD04G1018700.v1.1 MD04G1018900.v1.1
prunus_persica	Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G168200_v2.0.a1 Prupe.1G168200_v2.0.a1 Prupe.1G168200_v2.0.a1 Prupe.1G187500_v2.0.a1
pyrus_communis	pycom04g01500 pycom04g01510
rosa_chinensis	RchiOBHm_Chr4g0412911 RchiOBHm_Chr4g0413021 RchiOBHm_Chr4g0413231
rosa_laevigata	RLG00000008230 RLG00000008244 RLG00000008246
rosa_multiflora	Rmu_sc0000717.1_g000026 Rmu_sc0005849.1_g000011 Rmu_sc0021560.1_g000001
rosa_roxburghii	Rroxscaffold_5G00357150 Rroxscaffold_5G00357210
rosa_rugosa	Rorug04G0117500 Rorug04G0117600 Rorug04G0117700 Rorug04G0117800 Rorug04G0117900 Rorug04G0118000 Rorug04G0118000 Rorug04G0118100 Rorug04G0118100 Rorug04G0118600
rosa_samantha	Rh4AG176400 Rh4AG177100 Rh4AG178600 Rh4BG177000 Rh4BG177900 Rh4CG187400 Rh4CG188100 Rh4CG189200 Rh4DG173700 Rh4DG173900
rosa_wichuraiana	Rw4G014810 Rw4G014820 Rw4G014850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	417
AasI	GACNNNNNNGTC	1 cut(s)	795
AccBSI	CCGCTC	1 cut(s)	1656
AccI	GTMKAC	2 cut(s)	1196, 1395
AccII	CGCG	3 cut(s)	1193, 1342, 2135
AciI	CCGC	8 cut(s)	105, 147, 1087, 1167, 1191, 1413, 1656, 2135
AclI	AACGTT	1 cut(s)	1872
AclWI	GGATC	5 cut(s)	377, 390, 1241, 1271, 1953
AcoI	YGGCCR	3 cut(s)	54, 546, 1939
AcuI	CTGAAG	5 cut(s)	101, 285, 330, 471, 2082
AcyI	GRCGYC	1 cut(s)	1954
AfaI	GTAC	2 cut(s)	972, 1247
AfiI	CCNNNNNNNGG	3 cut(s)	980, 1086, 2203
AflIII	ACRYGT	3 cut(s)	274, 1055, 1465
AgeI	ACCGGT	2 cut(s)	1628, 1879
AgsI	TTSAA	9 cut(s)	661, 1409, 1609, 1788, 1829, 1877, 1922, 2114, 2239
AhlI	ACTAGT	1 cut(s)	1390
AjiI	CACGTC	2 cut(s)	1495, 1835
AjnI	CCWGG	5 cut(s)	84, 267, 1645, 1708, 1941
AleI	CACNNNNGTG	1 cut(s)	1082
AloI	GAACNNNNNNTCC	1 cut(s)	28
AluBI	AGCT	7 cut(s)	93, 350, 424, 1034, 1210, 1898, 2083
AluI	AGCT	7 cut(s)	93, 350, 424, 1034, 1210, 1898, 2083
Alw26I	GTCTC	2 cut(s)	1520, 1752
AlwI	GGATC	5 cut(s)	377, 390, 1241, 1271, 1953
AlwNI	CAGNNNCTG	2 cut(s)	308, 329
AoxI	GGCC	7 cut(s)	54, 546, 1364, 1431, 1939, 2041, 2161
ApeKI	GCWGC	7 cut(s)	90, 350, 1352, 1898, 2000, 2015, 2027
AsiGI	ACCGGT	2 cut(s)	1628, 1879
Asp700I	GAANNNNTTC	1 cut(s)	256
AspA2I	CCTAGG	1 cut(s)	958
AspLEI	GCGC	4 cut(s)	73, 634, 1342, 1715
AspS9I	GGNCC	3 cut(s)	1676, 2120, 2194
AsuHPI	GGTGA	5 cut(s)	252, 374, 580, 1643, 1772
AvaII	GGWCC	3 cut(s)	1676, 2120, 2194
AvrII	CCTAGG	1 cut(s)	958
BaeGI	GKGCMC	1 cut(s)	111
BaeI	ACNNNNGTAYC	2 cut(s)	1292, 1325
BalI	TGGCCA	2 cut(s)	56, 548
BamHI	GGATCC	1 cut(s)	382
BarI	GAAGNNNNNNTAC	4 cut(s)	1682, 1714, 1977, 2009
BbvI	GCAGC	7 cut(s)	102, 337, 1339, 1885, 2002, 2012, 2014
BccI	CCATC	1 cut(s)	1733
BceAI	ACGGC	1 cut(s)	501
BcgI	CGANNNNNNTGC	2 cut(s)	473, 507
BciT130I	CCWGG	5 cut(s)	86, 269, 1647, 1710, 1943
BciVI	GTATCC	2 cut(s)	1578, 1688
BclI	TGATCA	1 cut(s)	532
BcoDI	GTCTC	2 cut(s)	1520, 1752
BcuI	ACTAGT	1 cut(s)	1390
BfaI	CTAG	6 cut(s)	284, 368, 779, 959, 1391, 1748
BfmI	CTRYAG	3 cut(s)	1396, 1502, 2019
BfoI	RGCGCY	2 cut(s)	74, 1716
BfuI	GTATCC	2 cut(s)	1578, 1688
BglII	AGATCT	1 cut(s)	341
BisI	GCNGC	7 cut(s)	91, 351, 1353, 1899, 2001, 2016, 2028
BlnI	CCTAGG	1 cut(s)	958
BlsI	GCNGC	7 cut(s)	92, 352, 1354, 1900, 2002, 2017, 2029
Bme1390I	CCNGG	5 cut(s)	86, 269, 1647, 1710, 1943
Bme18I	GGWCC	3 cut(s)	1676, 2120, 2194
BmgBI	CACGTC	2 cut(s)	1495, 1835
BmgT120I	GGNCC	3 cut(s)	1676, 2120, 2194
BmiI	GGNNCC	5 cut(s)	384, 753, 1292, 1677, 2195
BmrFI	CCNGG	5 cut(s)	86, 269, 1647, 1710, 1943
BmsI	GCATC	6 cut(s)	286, 952, 955, 1446, 2014, 2023
BpmI	CTGGAG	2 cut(s)	573, 1776
Bpu10I	CCTNAGC	1 cut(s)	2045
BpuEI	CTTGAG	3 cut(s)	446, 1665, 1924
BsaAI	YACGTR	2 cut(s)	843, 1056
BsaBI	GATNNNNATC	2 cut(s)	387, 2129
BsaHI	GRCGYC	1 cut(s)	1954
BsaJI	CCNNGG	7 cut(s)	85, 181, 687, 958, 1013, 1839, 1957
BsaWI	WCCGGW	2 cut(s)	1628, 1879
BsaXI	ACNNNNNCTCC	2 cut(s)	1803, 1833
Bsc4I	CCNNNNNNNGG	3 cut(s)	980, 1086, 2203
Bse118I	RCCGGY	3 cut(s)	1433, 1628, 1879
Bse1I	ACTGG	4 cut(s)	223, 556, 1080, 1759
Bse3DI	GCAATG	1 cut(s)	1018
Bse8I	GATNNNNATC	2 cut(s)	387, 2129
BseBI	CCWGG	5 cut(s)	86, 269, 1647, 1710, 1943
BseDI	CCNNGG	7 cut(s)	85, 181, 687, 958, 1013, 1839, 1957
BseGI	GGATG	7 cut(s)	45, 462, 868, 970, 1105, 1187, 1738
BseJI	GATNNNNATC	2 cut(s)	387, 2129
BseLI	CCNNNNNNNGG	3 cut(s)	980, 1086, 2203
BseMI	GCAATG	1 cut(s)	1018
BseMII	CTCAG	3 cut(s)	642, 1593, 2075
BseNI	ACTGG	4 cut(s)	223, 556, 1080, 1759
BseRI	GAGGAG	2 cut(s)	1461, 1767
BseSI	GKGCMC	1 cut(s)	111
BseXI	GCAGC	7 cut(s)	102, 337, 1339, 1885, 2002, 2012, 2014
BseYI	CCCAGC	1 cut(s)	1378
Bsh1236I	CGCG	3 cut(s)	1193, 1342, 2135
BshFI	GGCC	7 cut(s)	56, 548, 1366, 1433, 1941, 2043, 2163
BshTI	ACCGGT	2 cut(s)	1628, 1879
BsiSI	CCGG	4 cut(s)	1434, 1629, 1880, 2040
BslFI	GGGAC	3 cut(s)	801, 1689, 2207
BslI	CCNNNNNNNGG	3 cut(s)	980, 1086, 2203
BsmAI	GTCTC	2 cut(s)	1520, 1752
BsmFI	GGGAC	3 cut(s)	801, 1689, 2207
BsmI	GAATGC	1 cut(s)	256
BsnI	GGCC	7 cut(s)	56, 548, 1366, 1433, 1941, 2043, 2163
Bsp1286I	GDGCHC	1 cut(s)	111
Bsp143I	GATC	8 cut(s)	341, 382, 532, 1233, 1263, 1553, 1945, 2124
Bsp19I	CCATGG	1 cut(s)	1013
BspACI	CCGC	8 cut(s)	105, 147, 1087, 1167, 1191, 1413, 1656, 2135
BspANI	GGCC	7 cut(s)	56, 548, 1366, 1433, 1941, 2043, 2163
BspCNI	CTCAG	3 cut(s)	643, 1594, 2076
BspFNI	CGCG	3 cut(s)	1193, 1342, 2135
BspHI	TCATGA	1 cut(s)	391
BspLI	GGNNCC	5 cut(s)	384, 753, 1292, 1677, 2195
BspMAI	CTGCAG	1 cut(s)	1506
BspPI	GGATC	5 cut(s)	377, 390, 1241, 1271, 1953
BspQI	GCTCTTC	2 cut(s)	100, 2073
BsrBI	CCGCTC	1 cut(s)	1656
BsrDI	GCAATG	1 cut(s)	1018
BsrFI	RCCGGY	3 cut(s)	1433, 1628, 1879
BsrI	ACTGG	4 cut(s)	223, 556, 1080, 1759
BssAI	RCCGGY	3 cut(s)	1433, 1628, 1879
BssECI	CCNNGG	7 cut(s)	85, 181, 687, 958, 1013, 1839, 1957
BssMI	GATC	8 cut(s)	341, 382, 532, 1233, 1263, 1553, 1945, 2124
BssNI	GRCGYC	1 cut(s)	1954
BssT1I	CCWWGG	5 cut(s)	687, 958, 1013, 1839, 1957
Bst2UI	CCWGG	5 cut(s)	86, 269, 1647, 1710, 1943
Bst4CI	ACNGT	6 cut(s)	498, 647, 834, 1201, 1400, 2159
Bst6I	CTCTTC	4 cut(s)	100, 588, 1980, 2073
BstACI	GRCGYC	1 cut(s)	1954
BstAPI	GCANNNNNTGC	1 cut(s)	500
BstBAI	YACGTR	2 cut(s)	843, 1056
BstC8I	GCNNGC	4 cut(s)	107, 636, 1004, 1435
BstDEI	CTNAG	6 cut(s)	67, 651, 998, 1602, 2045, 2084
BstDSI	CCRYGG	1 cut(s)	1013
BstEII	GGTNACC	1 cut(s)	362
BstENI	CCTNNNNNAGG	1 cut(s)	978
BstF5I	GGATG	7 cut(s)	45, 462, 868, 970, 1105, 1187, 1738
BstFNI	CGCG	3 cut(s)	1193, 1342, 2135
BstH2I	RGCGCY	2 cut(s)	74, 1716
BstHHI	GCGC	4 cut(s)	73, 634, 1342, 1715
BstKTI	GATC	8 cut(s)	344, 385, 535, 1236, 1266, 1556, 1948, 2127
BstMAI	GTCTC	2 cut(s)	1520, 1752
BstMBI	GATC	8 cut(s)	341, 382, 532, 1233, 1263, 1553, 1945, 2124
BstMWI	GCNNNNNNNGC	6 cut(s)	500, 554, 1216, 1997, 2024, 2033
BstNI	CCWGG	5 cut(s)	86, 269, 1647, 1710, 1943
BstNSI	RCATGY	3 cut(s)	1006, 1376, 1469
BstPI	GGTNACC	1 cut(s)	362
BstSCI	CCNGG	5 cut(s)	84, 267, 1645, 1708, 1941
BstSFI	CTRYAG	3 cut(s)	1396, 1502, 2019
BstSLI	GKGCMC	1 cut(s)	111
BstSNI	TACGTA	1 cut(s)	843
BstUI	CGCG	3 cut(s)	1193, 1342, 2135
BstV1I	GCAGC	7 cut(s)	102, 337, 1339, 1885, 2002, 2012, 2014
BstX2I	RGATCY	3 cut(s)	341, 382, 1263
BstXI	CCANNNNNNTGG	1 cut(s)	2011
BstYI	RGATCY	3 cut(s)	341, 382, 1263
BsuI	GTATCC	2 cut(s)	1578, 1688
BsuRI	GGCC	7 cut(s)	56, 548, 1366, 1433, 1941, 2043, 2163
BtgI	CCRYGG	1 cut(s)	1013
BtgZI	GCGATG	1 cut(s)	707
BtrI	CACGTC	2 cut(s)	1495, 1835
BtsCI	GGATG	7 cut(s)	45, 462, 868, 970, 1105, 1187, 1738
BtsIMutI	CAGTG	6 cut(s)	494, 549, 1087, 1447, 1752, 2164
Cac8I	GCNNGC	4 cut(s)	107, 636, 1004, 1435
CaiI	CAGNNNCTG	2 cut(s)	308, 329
CciI	TCATGA	1 cut(s)	391
CfoI	GCGC	4 cut(s)	73, 634, 1342, 1715
Cfr10I	RCCGGY	3 cut(s)	1433, 1628, 1879
Cfr13I	GGNCC	3 cut(s)	1676, 2120, 2194
CpoI	CGGWCCG	1 cut(s)	2120
CseI	GACGC	2 cut(s)	1182, 1962
Csp6I	GTAC	2 cut(s)	971, 1246
CspAI	ACCGGT	2 cut(s)	1628, 1879
CspCI	CAANNNNNGTGG	2 cut(s)	546, 581
CspI	CGGWCCG	1 cut(s)	2120
CviAII	CATG	9 cut(s)	392, 513, 919, 1003, 1014, 1249, 1373, 1466, 2179
CviQI	GTAC	2 cut(s)	971, 1246
DdeI	CTNAG	6 cut(s)	67, 651, 998, 1602, 2045, 2084
DpnI	GATC	8 cut(s)	343, 384, 534, 1235, 1265, 1555, 1947, 2126
DpnII	GATC	8 cut(s)	341, 382, 532, 1233, 1263, 1553, 1945, 2124
DrdI	GACNNNNNNGTC	1 cut(s)	795
DseDI	GACNNNNNNGTC	1 cut(s)	795
EaeI	YGGCCR	3 cut(s)	54, 546, 1939
Eam1104I	CTCTTC	4 cut(s)	100, 588, 1980, 2073
EarI	CTCTTC	4 cut(s)	100, 588, 1980, 2073
EciI	GGCGGA	1 cut(s)	1402
Eco105I	TACGTA	1 cut(s)	843
Eco130I	CCWWGG	5 cut(s)	687, 958, 1013, 1839, 1957
Eco32I	GATATC	1 cut(s)	669
Eco47I	GGWCC	3 cut(s)	1676, 2120, 2194
Eco57I	CTGAAG	5 cut(s)	101, 285, 330, 471, 2082
Eco91I	GGTNACC	1 cut(s)	362
EcoNI	CCTNNNNNAGG	1 cut(s)	978
EcoO109I	RGGNCCY	1 cut(s)	1676
EcoO65I	GGTNACC	1 cut(s)	362
EcoRI	GAATTC	1 cut(s)	1659
EcoRII	CCWGG	5 cut(s)	84, 267, 1645, 1708, 1941
EcoRV	GATATC	1 cut(s)	669
EcoT14I	CCWWGG	5 cut(s)	687, 958, 1013, 1839, 1957
ErhI	CCWWGG	5 cut(s)	687, 958, 1013, 1839, 1957
FaeI	CATG	9 cut(s)	395, 516, 922, 1006, 1017, 1252, 1376, 1469, 2182
FaqI	GGGAC	3 cut(s)	801, 1689, 2207
FatI	CATG	9 cut(s)	391, 512, 918, 1002, 1013, 1248, 1372, 1465, 2178
FauI	CCCGC	1 cut(s)	98
FbaI	TGATCA	1 cut(s)	532
FblI	GTMKAC	2 cut(s)	1196, 1395
Fnu4HI	GCNGC	7 cut(s)	91, 351, 1353, 1899, 2001, 2016, 2028
FokI	GGATG	7 cut(s)	32, 469, 875, 977, 1112, 1174, 1745
Fsp4HI	GCNGC	7 cut(s)	91, 351, 1353, 1899, 2001, 2016, 2028
FspBI	CTAG	6 cut(s)	284, 368, 779, 959, 1391, 1748
GlaI	GCGC	4 cut(s)	72, 633, 1341, 1714
GluI	GCNGC	7 cut(s)	91, 351, 1353, 1899, 2001, 2016, 2028
GsaI	CCCAGC	1 cut(s)	1382
GsuI	CTGGAG	2 cut(s)	573, 1776
HaeII	RGCGCY	2 cut(s)	74, 1716
HaeIII	GGCC	7 cut(s)	56, 548, 1366, 1433, 1941, 2043, 2163
HapII	CCGG	4 cut(s)	1434, 1629, 1880, 2040
HgaI	GACGC	2 cut(s)	1182, 1962
HhaI	GCGC	4 cut(s)	73, 634, 1342, 1715
Hin1I	GRCGYC	1 cut(s)	1954
Hin1II	CATG	9 cut(s)	395, 516, 922, 1006, 1017, 1252, 1376, 1469, 2182
Hin6I	GCGC	4 cut(s)	71, 632, 1340, 1713
HinP1I	GCGC	4 cut(s)	71, 632, 1340, 1713
HindIII	AAGCTT	1 cut(s)	1208
HinfI	GANTC	9 cut(s)	305, 775, 796, 815, 1154, 1619, 1705, 2170, 2199
HpaII	CCGG	4 cut(s)	1434, 1629, 1880, 2040
HphI	GGTGA	5 cut(s)	252, 374, 580, 1643, 1772
Hpy166II	GTNNAC	3 cut(s)	643, 1197, 1396
Hpy188III	TCNNGA	7 cut(s)	141, 329, 392, 650, 779, 1548, 1826
Hpy8I	GTNNAC	3 cut(s)	643, 1197, 1396
Hpy99I	CGWCG	3 cut(s)	1554, 1956, 2193
HpyCH4III	ACNGT	6 cut(s)	498, 647, 834, 1201, 1400, 2159
HpyCH4IV	ACGT	8 cut(s)	81, 274, 842, 1055, 1277, 1494, 1834, 1872
HpyCH4V	TGCA	5 cut(s)	494, 604, 943, 1372, 1504
HpyF10VI	GCNNNNNNNGC	6 cut(s)	500, 554, 1216, 1997, 2024, 2033
HpyF3I	CTNAG	6 cut(s)	67, 651, 998, 1602, 2045, 2084
HpySE526I	ACGT	8 cut(s)	81, 274, 842, 1055, 1277, 1494, 1834, 1872
Hsp92I	GRCGYC	1 cut(s)	1954
Hsp92II	CATG	9 cut(s)	395, 516, 922, 1006, 1017, 1252, 1376, 1469, 2182
HspAI	GCGC	4 cut(s)	71, 632, 1340, 1713
KroI	GCCGGC	1 cut(s)	1433
KroNI	GCCGGC	1 cut(s)	1435
Ksp22I	TGATCA	1 cut(s)	532
Kzo9I	GATC	8 cut(s)	341, 382, 532, 1233, 1263, 1553, 1945, 2124
LguI	GCTCTTC	2 cut(s)	100, 2073
LmnI	GCTCC	2 cut(s)	554, 1780
Lsp1109I	GCAGC	7 cut(s)	102, 337, 1339, 1885, 2002, 2012, 2014
LweI	GCATC	6 cut(s)	286, 952, 955, 1446, 2014, 2023
MaeI	CTAG	6 cut(s)	284, 368, 779, 959, 1391, 1748
MaeII	ACGT	8 cut(s)	81, 274, 842, 1055, 1277, 1494, 1834, 1872
MaeIII	GTNAC	4 cut(s)	279, 362, 597, 1155
MalI	GATC	8 cut(s)	343, 384, 534, 1235, 1265, 1555, 1947, 2126
MbiI	CCGCTC	1 cut(s)	1656
MboI	GATC	8 cut(s)	341, 382, 532, 1233, 1263, 1553, 1945, 2124
MboII	GAAGA	7 cut(s)	87, 248, 336, 605, 686, 1997, 2090
MfeI	CAATTG	2 cut(s)	1488, 1965
MflI	RGATCY	3 cut(s)	341, 382, 1263
MhlI	GDGCHC	1 cut(s)	111
MlsI	TGGCCA	2 cut(s)	56, 548
MluNI	TGGCCA	2 cut(s)	56, 548
MlyI	GAGTC	4 cut(s)	805, 1163, 2164, 2208
MmeI	TCCRAC	4 cut(s)	153, 448, 1748, 2198
Mox20I	TGGCCA	2 cut(s)	56, 548
MroNI	GCCGGC	1 cut(s)	1433
MroXI	GAANNNNTTC	1 cut(s)	256
MscI	TGGCCA	2 cut(s)	56, 548
MseI	TTAA	5 cut(s)	855, 1257, 1520, 1563, 1859
MslI	CAYNNNNRTG	3 cut(s)	50, 1023, 1082
Msp20I	TGGCCA	2 cut(s)	56, 548
MspA1I	CMGCKG	2 cut(s)	350, 1167
MspI	CCGG	4 cut(s)	1434, 1629, 1880, 2040
MspR9I	CCNGG	5 cut(s)	86, 269, 1647, 1710, 1943
MunI	CAATTG	2 cut(s)	1488, 1965
Mva1269I	GAATGC	1 cut(s)	256
MvaI	CCWGG	5 cut(s)	86, 269, 1647, 1710, 1943
MvnI	CGCG	3 cut(s)	1193, 1342, 2135
MwoI	GCNNNNNNNGC	6 cut(s)	500, 554, 1216, 1997, 2024, 2033
NaeI	GCCGGC	1 cut(s)	1435
NcoI	CCATGG	1 cut(s)	1013
NdeII	GATC	8 cut(s)	341, 382, 532, 1233, 1263, 1553, 1945, 2124
NgoMIV	GCCGGC	1 cut(s)	1433
NlaIII	CATG	9 cut(s)	395, 516, 922, 1006, 1017, 1252, 1376, 1469, 2182
NlaIV	GGNNCC	5 cut(s)	384, 753, 1292, 1677, 2195
NmeAIII	GCCGAG	1 cut(s)	206
NmuCI	GTSAC	2 cut(s)	362, 1155
NspI	RCATGY	3 cut(s)	1006, 1376, 1469
OliI	CACNNNNGTG	1 cut(s)	1082
PaeI	GCATGC	1 cut(s)	1006
PagI	TCATGA	1 cut(s)	391
PciI	ACATGT	1 cut(s)	1465
PciSI	GCTCTTC	2 cut(s)	100, 2073
PcsI	WCGNNNNNNNCGW	2 cut(s)	1283, 2194
PctI	GAATGC	1 cut(s)	256
PdiI	GCCGGC	1 cut(s)	1435
PdmI	GAANNNNTTC	1 cut(s)	256
PfeI	GAWTC	5 cut(s)	305, 775, 815, 1619, 1705
PflFI	GACNNNGTC	1 cut(s)	2198
PfoI	TCCNGGA	1 cut(s)	267
PinAI	ACCGGT	2 cut(s)	1628, 1879
PkrI	GCNGC	7 cut(s)	92, 352, 1354, 1900, 2002, 2017, 2029
PleI	GAGTC	4 cut(s)	804, 1162, 2164, 2207
PpsI	GAGTC	4 cut(s)	804, 1162, 2164, 2207
Ppu21I	YACGTR	2 cut(s)	843, 1056
PpuMI	RGGWCCY	1 cut(s)	1676
PscI	ACATGT	1 cut(s)	1465
PsiI	TTATAA	1 cut(s)	417
Psp1406I	AACGTT	1 cut(s)	1872
Psp5II	RGGWCCY	1 cut(s)	1676
Psp6I	CCWGG	5 cut(s)	84, 267, 1645, 1708, 1941
PspEI	GGTNACC	1 cut(s)	362
PspFI	CCCAGC	1 cut(s)	1378
PspGI	CCWGG	5 cut(s)	84, 267, 1645, 1708, 1941
PspN4I	GGNNCC	5 cut(s)	384, 753, 1292, 1677, 2195
PspPI	GGNCC	3 cut(s)	1676, 2120, 2194
PspPPI	RGGWCCY	1 cut(s)	1676
PsrI	GAACNNNNNNTAC	2 cut(s)	264, 296
PstI	CTGCAG	1 cut(s)	1506
PstNI	CAGNNNCTG	2 cut(s)	308, 329
PsuI	RGATCY	3 cut(s)	341, 382, 1263
PsyI	GACNNNGTC	1 cut(s)	2198
PvuII	CAGCTG	1 cut(s)	350
RsaI	GTAC	2 cut(s)	972, 1247
RsaNI	GTAC	2 cut(s)	971, 1246
RseI	CAYNNNNRTG	3 cut(s)	50, 1023, 1082
Rsr2I	CGGWCCG	1 cut(s)	2120
RsrII	CGGWCCG	1 cut(s)	2120
SapI	GCTCTTC	2 cut(s)	100, 2073
SaqAI	TTAA	5 cut(s)	855, 1257, 1520, 1563, 1859
SatI	GCNGC	7 cut(s)	91, 351, 1353, 1899, 2001, 2016, 2028
Sau3AI	GATC	8 cut(s)	341, 382, 532, 1233, 1263, 1553, 1945, 2124
Sau96I	GGNCC	3 cut(s)	1676, 2120, 2194
SchI	GAGTC	4 cut(s)	805, 1163, 2164, 2208
ScrFI	CCNGG	5 cut(s)	86, 269, 1647, 1710, 1943
SduI	GDGCHC	1 cut(s)	111
SfaNI	GCATC	6 cut(s)	286, 952, 955, 1446, 2014, 2023
SfcI	CTRYAG	3 cut(s)	1396, 1502, 2019
SinI	GGWCC	3 cut(s)	1676, 2120, 2194
SmiMI	CAYNNNNRTG	3 cut(s)	50, 1023, 1082
SmlI	CTYRAG	3 cut(s)	425, 1680, 1903
SmoI	CTYRAG	3 cut(s)	425, 1680, 1903
SnaBI	TACGTA	1 cut(s)	843
SpeI	ACTAGT	1 cut(s)	1390
SphI	GCATGC	1 cut(s)	1006
SsiI	CCGC	8 cut(s)	105, 147, 1087, 1167, 1191, 1413, 1656, 2135
SspMI	CTAG	6 cut(s)	284, 368, 779, 959, 1391, 1748
StyD4I	CCNGG	5 cut(s)	84, 267, 1645, 1708, 1941
StyI	CCWWGG	5 cut(s)	687, 958, 1013, 1839, 1957
TaaI	ACNGT	6 cut(s)	498, 647, 834, 1201, 1400, 2159
TaiI	ACGT	8 cut(s)	84, 277, 845, 1058, 1280, 1497, 1837, 1875
TaqI	TCGA	8 cut(s)	404, 578, 591, 762, 1482, 1549, 1594, 2188
TaqII	GACCGA	2 cut(s)	2137, 2211
TfiI	GAWTC	5 cut(s)	305, 775, 815, 1619, 1705
Tru1I	TTAA	5 cut(s)	855, 1257, 1520, 1563, 1859
Tru9I	TTAA	5 cut(s)	855, 1257, 1520, 1563, 1859
TscAI	CASTG	6 cut(s)	501, 556, 1087, 1447, 1759, 2164
TseFI	GTSAC	2 cut(s)	362, 1155
TseI	GCWGC	7 cut(s)	90, 350, 1352, 1898, 2000, 2015, 2027
Tsp45I	GTSAC	2 cut(s)	362, 1155
TspGWI	ACGGA	3 cut(s)	4, 113, 189
TspRI	CASTG	6 cut(s)	501, 556, 1087, 1447, 1759, 2164
Tth111I	GACNNNGTC	1 cut(s)	2198
VpaK11BI	GGWCC	3 cut(s)	1676, 2120, 2194
XagI	CCTNNNNNAGG	1 cut(s)	978
XbaI	TCTAGA	1 cut(s)	778
XceI	RCATGY	3 cut(s)	1006, 1376, 1469
XmaJI	CCTAGG	1 cut(s)	958
XmiI	GTMKAC	2 cut(s)	1196, 1395
XmnI	GAANNNNTTC	1 cut(s)	256
XspI	CTAG	6 cut(s)	284, 368, 779, 959, 1391, 1748

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.