Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001017

Physical Location & Seq

Genomic Coordinates Reverse (-)

2173388 .. 2182982

Gene Structure 9595 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1018900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2232 bp

ATGCTTTCTTCATCTGCTGCTTCTGCAAATCAAATTGGCGCCACCTTTTCCTTGAGGCCCAACTTGTTGTTGGCAAGCTCTTCTGATTGTGGGGGTGCCCTTCAATTCCTGTTTTCTAGCTACAGTTTCAGAAGCAGATGTGTTTTTCTTCGAACATGGCATGGAAGGCGAGGTGGGTTTCTTCCGAATCGCGGGTTTCGAGTGTTTTGCGAATCAAAGACAGAGGAACTGCAGATAAGAAGGTATTCACCATTTCTAGAAAGTGCGTTCCTAAACAGGAATGGTGCTTCTGTATCTGATGAGTGGCAGGCAGTTCCTGACATTTGGAGATCTTCAGCCGAGAGATATGGTGATCGTGTAGCATTGACAGATCCATACCATGACCCGCCTTCAAGCATGACTTATAAACAGCTGGAAGAGGAAATTTTGGATTTCTCTGAAGGCCTAAGAGTTGTTGGAGTAAAGCCTGAAGACAAAATTGCACTTTTTGCCAATAATTCATGTCGTTGGCTTGTAGCAGATCAGGGTATAATGGCTACTGGAGCTATCAATGTGGTAAGAGGTTCAAGGTCTTCAGTGGAAGAGTTGTTACAAATATATGATCACTCTGAAAGTGTCGCACTTGCCGTGGACAGTCCTGAATTGTTCAATCAGATCGCAGAAGCATTCTCTTCCAAGGTTGTCACGAAATTTGTAATTCTGCTTTGGGGGGAGAAGTCAAGCCTGGCCAGTGAAGAGAAGACTCCTGTTTTTAACTACAAGGAGATTCTGGATCTGGGTCGAGAGAGTCGTAAGAGTATGCTCGACTTTAATGATGCCCGGGGAAAGTATACATATGAAACTATCAACTCAGATGATGTAGCTACACTAGTATATACAAGCGGAACAACTGGGAACCCGAAAGGTGTCATGCTAACTCATCGAAATCTGTTGCATCAGATAAAAAACCTATGGGATGTTGTGCCTGCTGAAGCTGGGGACAGATTTCTCAGCATGCTTCCACCTTGGCATGCCTATGAACGAGCTTGTGAGTATTTCACATTGACGTACGGAATCGAGCAAGTATACACAACTGTCAGGAACTTGAAGGAGGATTTGCTACAGTATCAACCAAATTTCATAATTTCTGTTCCTTTAGTGTATGAGACACTTTACAGTGGGATTCAGAAACAGATCTCTATGAGCTCTACTGCTCGTAAGTTTATTGCACTTACATTCATAAGGATCAGTTTGGCATACATGGAGTTAAAGAGGATTTACGAGGGAACATATTTGACAAGGAACCAGAAGCAACCATCTTATCTTGCTTCACTGTATGATTGGCTATGGGCAAGAGTTGTTGCTGCGATATTATGGCCTTTGCATATGTTGGGAAAGAAGCTTGTCTATACTAAAATTCATTCTGCTGTCGGAATATCAAAGGCTGGTATTAGCGGAGGAGGTAGCTTGCCATCACATGTTGATAAGTTTTTTGAGGCAATTGGTGTGAAAGTGCAAAATGGATATGGTTTAACAGAGACTTCTCCAGTTATTGCTGCTCGCCGGCCAAACAATAATGTTCTTGGTTCAGTTGGGCCTCCAATTCGACATACCGAGTTCAAAGTTGTTGATTTGGAAACTGGTGAAGTTCTCCCACCAGGATCAAGTGGCATTATAAATGTTCGGGGCCCACAAGTGATGAAAGGATACTATAAGAATCCAGCGGCTACAAAGCAAGTACTAGATGAGGATGGTTGGCTGAGTACTGGAGATATTGGTTGGATTGCTCCTCACCACTCAACAGGGCGAAGTCGTCGTTGTGGAGGTGTTATTGTCCTGGAAGGACGCGCAAAGGATACTATAGTCCTTTCAACAGGTGAAAATGTTGAACCAGTGGAGCTTGAGGAAGCTGCCATGAGAAGTAGTCTAATTCAACAAATTGTTGTGGTCGGCCAGGATCAACGACGACTTGGGGCAATAATTGTTCCAAACAAAGAAGAGGTTTTACTTGCAGCCAAAAAGTTGTCCATTGTAGATGTTAATGCCTCTGAGCTCAGTATGGATAAAATGAAAAACTTGGTATATGAAGATTTAAGAAGATGGACTACAGGGTGTTCGTTTCAAATTGGACCGATTTTGATTGTGGATGAACCCTTCACGATTGATAGTGGACTAATGACTCCAACTATGAAAATTCGAAGGGACCGAGTCGTAGCTCAGTTCAAGGAGCAAATAAATAACCTGTACAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0008922 GO:0009058 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0015645 GO:0016053 GO:0016874 GO:0016877 GO:0016878 GO:0019752 GO:0030497 GO:0031967 GO:0031975 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00071 ko01100 ko01212 ko02024 ko03320 ko04146 ko04216 ko04714 ko04920 map00061 map00071 map01100 map01212 map02024 map03320 map04146 map04216 map04714 map04920

Pfam Domains

Protein Families

AMP-binding

Protein Analysis

744

Amino Acids

83.01

Weight (kDa)

7.98

Isoelectric Point (pI)

44.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	111 - 564	2.2e-80	AMP-binding enzyme
AMP-binding_C_3	PF23562	612 - 663	5e-10	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001017)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23790 AT4G14070
fragaria_vesca	FvH4_4g13140 FvH4_4g13151 FvH4_4g13151 FvH4_4g13151 FvH4_4g13151 FvH4_4g13151 FvH4_4g13151 FvH4_4g13151 FvH4_4g13151
malus_domestica	MD04G1018700.v1.1 MD04G1018900.v1.1
prunus_persica	Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G167500_v2.0.a1 Prupe.1G168200_v2.0.a1 Prupe.1G168200_v2.0.a1 Prupe.1G168200_v2.0.a1 Prupe.1G187500_v2.0.a1
pyrus_communis	pycom04g01500 pycom04g01510
rosa_chinensis	RchiOBHm_Chr4g0412911 RchiOBHm_Chr4g0413021 RchiOBHm_Chr4g0413231
rosa_laevigata	RLG00000008230 RLG00000008244 RLG00000008246
rosa_multiflora	Rmu_sc0000717.1_g000026 Rmu_sc0005849.1_g000011 Rmu_sc0021560.1_g000001
rosa_roxburghii	Rroxscaffold_5G00357150 Rroxscaffold_5G00357210
rosa_rugosa	Rorug04G0117500 Rorug04G0117600 Rorug04G0117700 Rorug04G0117800 Rorug04G0117900 Rorug04G0118000 Rorug04G0118000 Rorug04G0118100 Rorug04G0118100 Rorug04G0118600
rosa_samantha	Rh4AG176400 Rh4AG177100 Rh4AG178600 Rh4BG177000 Rh4BG177900 Rh4CG187400 Rh4CG188100 Rh4CG189200 Rh4DG173700 Rh4DG173900
rosa_wichuraiana	Rw4G014810 Rw4G014820 Rw4G014850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	405, 1655
AccB1I	GGYRCC	2 cut(s)	38, 95
AccI	GTMKAC	2 cut(s)	830, 1065
AccII	CGCG	2 cut(s)	192, 1827
AciI	CCGC	5 cut(s)	192, 386, 882, 1434, 1703
AclWI	GGATC	5 cut(s)	365, 780, 1232, 1648, 1944
AcoI	YGGCCR	3 cut(s)	726, 1544, 1930
AcsI	RAATTY	5 cut(s)	423, 689, 1114, 1395, 2172
AcuI	CTGAAG	5 cut(s)	318, 459, 489, 558, 990
AcyI	GRCGYC	1 cut(s)	39
AfaI	GTAC	4 cut(s)	1049, 1719, 1744, 2225
AfiI	CCNNNNNNNGG	1 cut(s)	191
AflIII	ACRYGT	1 cut(s)	1456
AhlI	ACTAGT	1 cut(s)	868
AjnI	CCWGG	4 cut(s)	723, 1636, 1815, 1932
Alw21I	GWGCWC	2 cut(s)	1187, 2034
Alw26I	GTCTC	2 cut(s)	1139, 1511
AlwI	GGATC	5 cut(s)	365, 780, 1232, 1648, 1944
AlwNI	CAGNNNCTG	1 cut(s)	317
Ama87I	CYCGRG	1 cut(s)	819
AoxI	GGCC	8 cut(s)	56, 442, 726, 1355, 1544, 1574, 1666, 1930
ApaI	GGGCCC	1 cut(s)	1670
ApeKI	GCWGC	5 cut(s)	17, 1343, 1535, 1889, 1991
ApoI	RAATTY	5 cut(s)	423, 689, 1114, 1395, 2172
Asp700I	GAANNNNTTC	1 cut(s)	244
AspLEI	GCGC	2 cut(s)	41, 1829
AspS9I	GGNCC	6 cut(s)	57, 1574, 1666, 1667, 2108, 2182
AsuC2I	CCSGG	2 cut(s)	820, 821
AsuHPI	GGTGA	5 cut(s)	240, 362, 1634, 1763, 1868
AsuII	TTCGAA	2 cut(s)	151, 2176
AvaI	CYCGRG	1 cut(s)	819
AvaII	GGWCC	2 cut(s)	2108, 2182
BaeGI	GKGCMC	2 cut(s)	100, 1670
BaeI	ACNNNNGTAYC	2 cut(s)	1827, 1860
BalI	TGGCCA	1 cut(s)	728
BanI	GGYRCC	2 cut(s)	38, 95
BanII	GRGCYC	3 cut(s)	1187, 1670, 2034
BarI	GAAGNNNNNNTAC	6 cut(s)	573, 605, 1968, 2000, 2068, 2100
BbsI	GAAGAC	3 cut(s)	477, 564, 746
Bbv12I	GWGCWC	2 cut(s)	1187, 2034
BbvI	GCAGC	5 cut(s)	4, 1330, 1522, 1876, 2003
BccI	CCATC	4 cut(s)	1303, 1459, 1724, 2073
BceAI	ACGGC	1 cut(s)	611
BcgI	CGANNNNNNTGC	2 cut(s)	189, 223
BciT130I	CCWGG	4 cut(s)	725, 1638, 1817, 1934
BciVI	GTATCC	2 cut(s)	1679, 1828
BclI	TGATCA	1 cut(s)	601
BcnI	CCSGG	2 cut(s)	820, 821
BcoDI	GTCTC	2 cut(s)	1139, 1511
BcuI	ACTAGT	1 cut(s)	868
BfaI	CTAG	4 cut(s)	117, 257, 869, 1721
BfmI	CTRYAG	5 cut(s)	121, 230, 1100, 1839, 2085
BfoI	RGCGCY	1 cut(s)	42
BfuI	GTATCC	2 cut(s)	1679, 1828
BglII	AGATCT	2 cut(s)	329, 1173
BisI	GCNGC	6 cut(s)	18, 1344, 1536, 1704, 1890, 1992
BlsI	GCNGC	6 cut(s)	19, 1345, 1537, 1705, 1891, 1993
BmcAI	AGTACT	2 cut(s)	1719, 1744
Bme1390I	CCNGG	6 cut(s)	725, 820, 821, 1638, 1817, 1934
Bme18I	GGWCC	2 cut(s)	2108, 2182
BmeT110I	CYCGRG	1 cut(s)	819
BmgT120I	GGNCC	6 cut(s)	57, 1574, 1666, 1667, 2108, 2182
BmiI	GGNNCC	7 cut(s)	40, 97, 896, 1283, 1667, 1668, 2183
BmrFI	CCNGG	6 cut(s)	725, 820, 821, 1638, 1817, 1934
BmrI	ACTGGG	1 cut(s)	900
BmsI	GCATC	2 cut(s)	805, 943
BmuI	ACTGGG	1 cut(s)	900
BpiI	GAAGAC	3 cut(s)	477, 564, 746
BpmI	CTGGAG	3 cut(s)	561, 1509, 1767
Bpu14I	TTCGAA	2 cut(s)	151, 2176
BpuEI	CTTGAG	2 cut(s)	73, 1901
BpuMI	CCSGG	2 cut(s)	820, 821
BsaHI	GRCGYC	1 cut(s)	39
BsaJI	CCNNGG	5 cut(s)	627, 675, 819, 820, 1004
Bsc4I	CCNNNNNNNGG	1 cut(s)	191
Bse118I	RCCGGY	1 cut(s)	1542
Bse1I	ACTGG	7 cut(s)	544, 729, 895, 1526, 1624, 1750, 1871
BseBI	CCWGG	4 cut(s)	725, 1638, 1817, 1934
BseDI	CCNNGG	5 cut(s)	627, 675, 819, 820, 1004
BseGI	GGATG	3 cut(s)	961, 1735, 2131
BseLI	CCNNNNNNNGG	1 cut(s)	191
BseMII	CTCAG	6 cut(s)	864, 1003, 1730, 2019, 2047, 2210
BseNI	ACTGG	7 cut(s)	544, 729, 895, 1526, 1624, 1750, 1871
BseRI	GAGGAG	2 cut(s)	1452, 1758
BseSI	GKGCMC	2 cut(s)	100, 1670
BseXI	GCAGC	5 cut(s)	4, 1330, 1522, 1876, 2003
BseYI	CCCAGC	1 cut(s)	974
Bsh1236I	CGCG	2 cut(s)	192, 1827
BshFI	GGCC	8 cut(s)	58, 444, 728, 1357, 1546, 1576, 1668, 1932
BshNI	GGYRCC	2 cut(s)	38, 95
BsiHKAI	GWGCWC	2 cut(s)	1187, 2034
BsiHKCI	CYCGRG	1 cut(s)	819
BsiSI	CCGG	2 cut(s)	820, 1543
BsiWI	CGTACG	1 cut(s)	1047
BslFI	GGGAC	2 cut(s)	992, 2195
BslI	CCNNNNNNNGG	1 cut(s)	191
BsmAI	GTCTC	2 cut(s)	1139, 1511
BsmFI	GGGAC	2 cut(s)	992, 2195
BsmI	GAATGC	1 cut(s)	665
BsnI	GGCC	8 cut(s)	58, 444, 728, 1357, 1546, 1576, 1668, 1932
BsoBI	CYCGRG	1 cut(s)	819
Bsp119I	TTCGAA	2 cut(s)	151, 2176
Bsp120I	GGGCCC	1 cut(s)	1666
Bsp1286I	GDGCHC	4 cut(s)	100, 1187, 1670, 2034
Bsp1407I	TGTACA	1 cut(s)	2223
BspACI	CCGC	5 cut(s)	192, 386, 882, 1434, 1703
BspANI	GGCC	8 cut(s)	58, 444, 728, 1357, 1546, 1576, 1668, 1932
BspCNI	CTCAG	6 cut(s)	863, 1002, 1731, 2020, 2046, 2209
BspFNI	CGCG	2 cut(s)	192, 1827
BspLI	GGNNCC	7 cut(s)	40, 97, 896, 1283, 1667, 1668, 2183
BspMAI	CTGCAG	1 cut(s)	234
BspPI	GGATC	5 cut(s)	365, 780, 1232, 1648, 1944
BspQI	GCTCTTC	1 cut(s)	85
BspT104I	TTCGAA	2 cut(s)	151, 2176
BspT107I	GGYRCC	2 cut(s)	38, 95
BsrFI	RCCGGY	1 cut(s)	1542
BsrGI	TGTACA	1 cut(s)	2223
BsrI	ACTGG	7 cut(s)	544, 729, 895, 1526, 1624, 1750, 1871
BssAI	RCCGGY	1 cut(s)	1542
BssECI	CCNNGG	5 cut(s)	627, 675, 819, 820, 1004
BssNAI	GTATAC	2 cut(s)	831, 1066
BssNI	GRCGYC	1 cut(s)	39
BssT1I	CCWWGG	2 cut(s)	675, 1004
Bst1107I	GTATAC	2 cut(s)	831, 1066
Bst2UI	CCWGG	4 cut(s)	725, 1638, 1817, 1934
Bst4CI	ACNGT	6 cut(s)	125, 635, 1075, 1104, 1157, 1314
Bst6I	CTCTTC	6 cut(s)	85, 411, 576, 676, 729, 1971
BstACI	GRCGYC	1 cut(s)	39
BstAPI	GCANNNNNTGC	1 cut(s)	488
BstAUI	TGTACA	1 cut(s)	2223
BstBI	TTCGAA	2 cut(s)	151, 2176
BstC8I	GCNNGC	8 cut(s)	76, 309, 966, 995, 1011, 1448, 1540, 1544
BstDEI	CTNAG	7 cut(s)	446, 850, 989, 1739, 2028, 2033, 2196
BstDSI	CCRYGG	1 cut(s)	627
BstF5I	GGATG	3 cut(s)	961, 1735, 2131
BstFNI	CGCG	2 cut(s)	192, 1827
BstH2I	RGCGCY	1 cut(s)	42
BstHHI	GCGC	2 cut(s)	41, 1829
BstMAI	GTCTC	2 cut(s)	1139, 1511
BstMWI	GCNNNNNNNGC	4 cut(s)	23, 166, 488, 542
BstNI	CCWGG	4 cut(s)	725, 1638, 1817, 1934
BstNSI	RCATGY	3 cut(s)	997, 1013, 1460
BstSCI	CCNGG	6 cut(s)	723, 818, 819, 1636, 1815, 1932
BstSFI	CTRYAG	5 cut(s)	121, 230, 1100, 1839, 2085
BstSLI	GKGCMC	2 cut(s)	100, 1670
BstUI	CGCG	2 cut(s)	192, 1827
BstV1I	GCAGC	5 cut(s)	4, 1330, 1522, 1876, 2003
BstV2I	GAAGAC	3 cut(s)	477, 564, 746
BstX2I	RGATCY	4 cut(s)	329, 370, 772, 1173
BstYI	RGATCY	4 cut(s)	329, 370, 772, 1173
BstZ17I	GTATAC	2 cut(s)	831, 1066
BsuI	GTATCC	2 cut(s)	1679, 1828
BsuRI	GGCC	8 cut(s)	58, 444, 728, 1357, 1546, 1576, 1668, 1932
BtgI	CCRYGG	1 cut(s)	627
BtsCI	GGATG	3 cut(s)	961, 1735, 2131
BtsIMutI	CAGTG	5 cut(s)	582, 736, 1162, 1310, 1878
Cac8I	GCNNGC	8 cut(s)	76, 309, 966, 995, 1011, 1448, 1540, 1544
CaiI	CAGNNNCTG	1 cut(s)	317
CfoI	GCGC	2 cut(s)	41, 1829
Cfr10I	RCCGGY	1 cut(s)	1542
Cfr13I	GGNCC	6 cut(s)	57, 1574, 1666, 1667, 2108, 2182
Cfr9I	CCCGGG	1 cut(s)	819
CseI	GACGC	1 cut(s)	1833
Csp6I	GTAC	4 cut(s)	1048, 1718, 1743, 2224
CviQI	GTAC	4 cut(s)	1048, 1718, 1743, 2224
DdeI	CTNAG	7 cut(s)	446, 850, 989, 1739, 2028, 2033, 2196
DinI	GGCGCC	1 cut(s)	40
EaeI	YGGCCR	3 cut(s)	726, 1544, 1930
Eam1104I	CTCTTC	6 cut(s)	85, 411, 576, 676, 729, 1971
EarI	CTCTTC	6 cut(s)	85, 411, 576, 676, 729, 1971
Ecl136II	GAGCTC	2 cut(s)	1185, 2032
Eco130I	CCWWGG	2 cut(s)	675, 1004
Eco147I	AGGCCT	1 cut(s)	444
Eco24I	GRGCYC	3 cut(s)	1187, 1670, 2034
Eco47I	GGWCC	2 cut(s)	2108, 2182
Eco53kI	GAGCTC	2 cut(s)	1185, 2032
Eco57I	CTGAAG	5 cut(s)	318, 459, 489, 558, 990
Eco88I	CYCGRG	1 cut(s)	819
EcoICRI	GAGCTC	2 cut(s)	1185, 2032
EcoO109I	RGGNCCY	1 cut(s)	1666
EcoRII	CCWGG	4 cut(s)	723, 1636, 1815, 1932
EcoT14I	CCWWGG	2 cut(s)	675, 1004
EcoT38I	GRGCYC	3 cut(s)	1187, 1670, 2034
EgeI	GGCGCC	1 cut(s)	40
EheI	GGCGCC	1 cut(s)	40
ErhI	CCWWGG	2 cut(s)	675, 1004
FalI	AAGNNNNNCTT	2 cut(s)	385, 417
FaqI	GGGAC	2 cut(s)	992, 2195
FauI	CCCGC	2 cut(s)	185, 393
FauNDI	CATATG	2 cut(s)	835, 1365
FbaI	TGATCA	1 cut(s)	601
FblI	GTMKAC	2 cut(s)	830, 1065
Fnu4HI	GCNGC	6 cut(s)	18, 1344, 1536, 1704, 1890, 1992
FokI	GGATG	3 cut(s)	968, 1742, 2138
FriOI	GRGCYC	3 cut(s)	1187, 1670, 2034
Fsp4HI	GCNGC	6 cut(s)	18, 1344, 1536, 1704, 1890, 1992
FspBI	CTAG	4 cut(s)	117, 257, 869, 1721
GlaI	GCGC	2 cut(s)	40, 1828
GluI	GCNGC	6 cut(s)	18, 1344, 1536, 1704, 1890, 1992
GsaI	CCCAGC	1 cut(s)	978
GsuI	CTGGAG	3 cut(s)	561, 1509, 1767
HaeII	RGCGCY	1 cut(s)	42
HaeIII	GGCC	8 cut(s)	58, 444, 728, 1357, 1546, 1576, 1668, 1932
HapII	CCGG	2 cut(s)	820, 1543
HgaI	GACGC	1 cut(s)	1833
HhaI	GCGC	2 cut(s)	41, 1829
Hin1I	GRCGYC	1 cut(s)	39
Hin6I	GCGC	2 cut(s)	39, 1827
HinP1I	GCGC	2 cut(s)	39, 1827
HindIII	AAGCTT	1 cut(s)	1379
HpaII	CCGG	2 cut(s)	820, 1543
HphI	GGTGA	5 cut(s)	240, 362, 1634, 1763, 1868
Hpy166II	GTNNAC	4 cut(s)	631, 831, 1066, 2150
Hpy188III	TCNNGA	8 cut(s)	257, 317, 638, 685, 770, 782, 1078, 2137
Hpy8I	GTNNAC	4 cut(s)	631, 831, 1066, 2150
Hpy99I	CGWCG	2 cut(s)	1797, 1947
HpyAV	CCTTC	9 cut(s)	110, 159, 234, 399, 434, 1081, 1814, 2143, 2172
HpyCH4III	ACNGT	6 cut(s)	125, 635, 1075, 1104, 1157, 1314
HpyCH4IV	ACGT	1 cut(s)	1046
HpyCH4V	TGCA	8 cut(s)	26, 232, 482, 934, 1208, 1363, 1495, 1991
HpyF10VI	GCNNNNNNNGC	4 cut(s)	23, 166, 488, 542
HpyF3I	CTNAG	7 cut(s)	446, 850, 989, 1739, 2028, 2033, 2196
HpySE526I	ACGT	1 cut(s)	1046
Hsp92I	GRCGYC	1 cut(s)	39
HspAI	GCGC	2 cut(s)	39, 1827
KasI	GGCGCC	1 cut(s)	38
KroI	GCCGGC	1 cut(s)	1542
KroNI	GCCGGC	1 cut(s)	1544
Ksp22I	TGATCA	1 cut(s)	601
LguI	GCTCTTC	1 cut(s)	85
LmnI	GCTCC	4 cut(s)	542, 1771, 1876, 2206
Lsp1109I	GCAGC	5 cut(s)	4, 1330, 1522, 1876, 2003
LweI	GCATC	2 cut(s)	805, 943
MaeI	CTAG	4 cut(s)	117, 257, 869, 1721
MaeII	ACGT	1 cut(s)	1046
MaeIII	GTNAC	2 cut(s)	588, 682
MfeI	CAATTG	1 cut(s)	1479
MflI	RGATCY	4 cut(s)	329, 370, 772, 1173
MhlI	GDGCHC	4 cut(s)	100, 1187, 1670, 2034
MlsI	TGGCCA	1 cut(s)	728
MluNI	TGGCCA	1 cut(s)	728
Mly113I	GGCGCC	1 cut(s)	39
MlyI	GAGTC	4 cut(s)	736, 796, 2152, 2196
MmeI	TCCRAC	4 cut(s)	436, 1390, 1739, 2186
Mox20I	TGGCCA	1 cut(s)	728
MroNI	GCCGGC	1 cut(s)	1542
MroXI	GAANNNNTTC	1 cut(s)	244
MscI	TGGCCA	1 cut(s)	728
MseI	TTAA	6 cut(s)	753, 810, 1247, 1511, 2019, 2072
MslI	CAYNNNNRTG	1 cut(s)	1014
Msp20I	TGGCCA	1 cut(s)	728
MspA1I	CMGCKG	2 cut(s)	412, 1703
MspI	CCGG	2 cut(s)	820, 1543
MspR9I	CCNGG	6 cut(s)	725, 820, 821, 1638, 1817, 1934
MunI	CAATTG	1 cut(s)	1479
Mva1269I	GAATGC	1 cut(s)	665
MvaI	CCWGG	4 cut(s)	725, 1638, 1817, 1934
MvnI	CGCG	2 cut(s)	192, 1827
MwoI	GCNNNNNNNGC	4 cut(s)	23, 166, 488, 542
NaeI	GCCGGC	1 cut(s)	1544
NarI	GGCGCC	1 cut(s)	39
NciI	CCSGG	2 cut(s)	820, 821
NdeI	CATATG	2 cut(s)	835, 1365
NgoMIV	GCCGGC	1 cut(s)	1542
NlaIV	GGNNCC	7 cut(s)	40, 97, 896, 1283, 1667, 1668, 2183
NmeAIII	GCCGAG	1 cut(s)	364
NmuCI	GTSAC	1 cut(s)	682
NspI	RCATGY	3 cut(s)	997, 1013, 1460
NspV	TTCGAA	2 cut(s)	151, 2176
PaeI	GCATGC	2 cut(s)	997, 1013
PceI	AGGCCT	1 cut(s)	444
PciI	ACATGT	1 cut(s)	1456
PciSI	GCTCTTC	1 cut(s)	85
PcsI	WCGNNNNNNNCGW	4 cut(s)	196, 624, 787, 2182
PctI	GAATGC	1 cut(s)	665
PdiI	GCCGGC	1 cut(s)	1544
PdmI	GAANNNNTTC	1 cut(s)	244
PfeI	GAWTC	6 cut(s)	187, 212, 766, 1053, 1162, 1696
Pfl23II	CGTACG	1 cut(s)	1047
PflFI	GACNNNGTC	1 cut(s)	2186
PfoI	TCCNGGA	1 cut(s)	1815
PkrI	GCNGC	6 cut(s)	19, 1345, 1537, 1705, 1891, 1993
PleI	GAGTC	4 cut(s)	736, 795, 2152, 2195
PluTI	GGCGCC	1 cut(s)	42
PpsI	GAGTC	4 cut(s)	736, 795, 2152, 2195
PscI	ACATGT	1 cut(s)	1456
PsiI	TTATAA	2 cut(s)	405, 1655
Psp124BI	GAGCTC	2 cut(s)	1187, 2034
Psp6I	CCWGG	4 cut(s)	723, 1636, 1815, 1932
PspFI	CCCAGC	1 cut(s)	974
PspGI	CCWGG	4 cut(s)	723, 1636, 1815, 1932
PspLI	CGTACG	1 cut(s)	1047
PspN4I	GGNNCC	7 cut(s)	40, 97, 896, 1283, 1667, 1668, 2183
PspOMI	GGGCCC	1 cut(s)	1666
PspPI	GGNCC	6 cut(s)	57, 1574, 1666, 1667, 2108, 2182
PstI	CTGCAG	1 cut(s)	234
PstNI	CAGNNNCTG	1 cut(s)	317
PsuI	RGATCY	4 cut(s)	329, 370, 772, 1173
PsyI	GACNNNGTC	1 cut(s)	2186
PvuII	CAGCTG	1 cut(s)	412
RsaI	GTAC	4 cut(s)	1049, 1719, 1744, 2225
RsaNI	GTAC	4 cut(s)	1048, 1718, 1743, 2224
RseI	CAYNNNNRTG	1 cut(s)	1014
SacI	GAGCTC	2 cut(s)	1187, 2034
SapI	GCTCTTC	1 cut(s)	85
SaqAI	TTAA	6 cut(s)	753, 810, 1247, 1511, 2019, 2072
SatI	GCNGC	6 cut(s)	18, 1344, 1536, 1704, 1890, 1992
Sau96I	GGNCC	6 cut(s)	57, 1574, 1666, 1667, 2108, 2182
ScaI	AGTACT	2 cut(s)	1719, 1744
SchI	GAGTC	4 cut(s)	736, 796, 2152, 2196
ScrFI	CCNGG	6 cut(s)	725, 820, 821, 1638, 1817, 1934
SduI	GDGCHC	4 cut(s)	100, 1187, 1670, 2034
SfaNI	GCATC	2 cut(s)	805, 943
SfcI	CTRYAG	5 cut(s)	121, 230, 1100, 1839, 2085
SfoI	GGCGCC	1 cut(s)	40
SfuI	TTCGAA	2 cut(s)	151, 2176
SinI	GGWCC	2 cut(s)	2108, 2182
SmaI	CCCGGG	1 cut(s)	821
SmiMI	CAYNNNNRTG	1 cut(s)	1014
SmlI	CTYRAG	2 cut(s)	52, 1880
SmoI	CTYRAG	2 cut(s)	52, 1880
SpeI	ACTAGT	1 cut(s)	868
SphI	GCATGC	2 cut(s)	997, 1013
SseBI	AGGCCT	1 cut(s)	444
SsiI	CCGC	5 cut(s)	192, 386, 882, 1434, 1703
SspDI	GGCGCC	1 cut(s)	38
SspMI	CTAG	4 cut(s)	117, 257, 869, 1721
SstI	GAGCTC	2 cut(s)	1187, 2034
StuI	AGGCCT	1 cut(s)	444
StyD4I	CCNGG	6 cut(s)	723, 818, 819, 1636, 1815, 1932
StyI	CCWWGG	2 cut(s)	675, 1004
TaaI	ACNGT	6 cut(s)	125, 635, 1075, 1104, 1157, 1314
TaiI	ACGT	1 cut(s)	1049
TaqI	TCGA	8 cut(s)	151, 199, 781, 804, 922, 1056, 1585, 2176
TaqII	GACCGA	2 cut(s)	2125, 2199
TatI	WGTACW	3 cut(s)	1717, 1742, 2223
TauI	GCSGC	1 cut(s)	1706
TfiI	GAWTC	6 cut(s)	187, 212, 766, 1053, 1162, 1696
Tru1I	TTAA	6 cut(s)	753, 810, 1247, 1511, 2019, 2072
Tru9I	TTAA	6 cut(s)	753, 810, 1247, 1511, 2019, 2072
TscAI	CASTG	5 cut(s)	582, 736, 1162, 1317, 1878
TseFI	GTSAC	1 cut(s)	682
TseI	GCWGC	5 cut(s)	17, 1343, 1535, 1889, 1991
Tsp45I	GTSAC	1 cut(s)	682
TspGWI	ACGGA	1 cut(s)	1065
TspMI	CCCGGG	1 cut(s)	819
TspRI	CASTG	5 cut(s)	582, 736, 1162, 1317, 1878
Tth111I	GACNNNGTC	1 cut(s)	2186
VpaK11BI	GGWCC	2 cut(s)	2108, 2182
XapI	RAATTY	5 cut(s)	423, 689, 1114, 1395, 2172
XbaI	TCTAGA	1 cut(s)	256
XceI	RCATGY	3 cut(s)	997, 1013, 1460
XcmI	CCANNNNNNNNNTGG	1 cut(s)	67
XmaI	CCCGGG	1 cut(s)	819
XmiI	GTMKAC	2 cut(s)	830, 1065
XmnI	GAANNNNTTC	1 cut(s)	244
XspI	CTAG	4 cut(s)	117, 257, 869, 1721
ZrmI	AGTACT	2 cut(s)	1719, 1744

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.