Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002902

Physical Location & Seq

Genomic Coordinates Forward (+)

5271939 .. 5279744

Gene Structure 7806 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1045300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1293 bp

ATGGTTTCCAGCAGAAAAATGAGAGAGCCAAAGATGAAGCTTCTTCTTCGAGCTCTGCAGCTTCTGCTTCCACTTCTAATCCTCCACTTCCGTTCCGCCACCTCTGAAATCTTCTTCGAAGAACGATTCGAAGATGGGTGGAAAAGCCGCTGGGTCTTATCAGACTGGAAAAGGGCAGAAGGAAAAGCAGGAACGTTCAAGTACACTGCAGGGAAATGGCCGGGGGATCCTGATGATAAAGGCATTCAAACATATAATGATGCCAAGCATTTTGCCATATCTGCTAAAGTACCTGAGTTCAGCAACAAAAATAGAACTCTAGTTGTCCAGTATTCGATTAAATTTGAGCAAGACATAGAATGTGGTGGTGGTTACATCAAGCTTCTCTCTAGCTATGTCAATCAGAAGAAGTTCGGTGGTGATACCCCGTACAGTTTCATGTTTGGACCAGATTTATGTGGCACACAGACAAAAAAACTCCATGTTATACTTTCCTACCAGGGGCAGAATTATCCCATAAAAAAGGATCTCCAATGTGAAACAGACAAGTTAACTCATTTCTATACATTTATTCTTAGGCCTGATGCAACTTACAGCGTCCTTGTTGATAATCGAGAGAGAGATTCTGGAAGCATGTATACAGATTGGGATATCCTTCCTCCAAAAAAAATAAAGGATGTGAACGCAAAAAAGCCAGCCGACTGGGATGACAGAGAGTATATTAATGACCCTAATGAAGTCAAACCGGAGGGCTATGATTCAATTCCATCTGAAATTCCTGATCCAAAAGCCAAAGAGCCCGAAGACTGGGATGAGGAAGAGAATGGAATTTGGAGGCAATCAAAGATCCCAAATCCAGCATACAAAGGGCCATGGAAGCGCAAGAAAATCAAGAACCCCAACTATAAGGGAAAGTGGAAGACTCCATGGATTGATAACCCCGAGTTTGAGGACGATCCTGACCTATATGTGCTGAAGCCAATTAAATATGTGGGCATTGAAGTTTGGCAGGTGAAGGCTGGTTCAGTTTTCGACAACATTTTAATTTGTGATGATCCACGGTATGCAAAAGAAGTTGTAGAAGAGGTTTTTTCCAATAGAGACGCTGAGAAAGAAGCTTTTGAGGAAGCAGAGAAGCAGAGGAAAGCTCGAGAAGAAGAGGAGGCTAAAAGAGCGAGAGAGGAAGGTGAAAAAAGGAAGAGGGAGAGGGATCGCGAATATGGCAGGGATAGGAGGCGCCGTAGAAGGCATGATCCCCATGATTATCTGGATGATTACCATGATGAACTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006457 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009812 GO:0009987 GO:0010204 GO:0012501 GO:0012505 GO:0016020 GO:0023052 GO:0031347 GO:0031349 GO:0031984 GO:0033554 GO:0034050 GO:0042175 GO:0042440 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046283 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071704 GO:0080134 GO:0098542 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko04141 ko04145 ko04612 ko05142 ko05166 map04141 map04145 map04612 map05142 map05166

Pfam Domains

Protein Families

Calreticulin

Protein Analysis

431

Amino Acids

50.77

Weight (kDa)

6.47

Isoelectric Point (pI)

47.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calreticulin	PF00262	39 - 275	2.9e-58	Calreticulin family
Calreticulin	PF00262	276 - 349	6.4e-19	Calreticulin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002902)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08450 AT1G08450 AT1G08450
fragaria_vesca	FvH4_1g23470 FvH4_1g23470 FvH4_1g23470 FvH4_1g23470 FvH4_1g23470 FvH4_6g00230 FvH4_6g00230 FvH4_6g00230
malus_domestica	MD04G1045300.v1.1 MD04G1247000.v1.1 MD12G1263200.v1.1
prunus_persica	Prupe.1G198500_v2.0.a1 Prupe.6G363200_v2.0.a1
pyrus_communis	pycom04g21770 pycom12g24070
rosa_chinensis	RchiOBHm_Chr2g0118841 RchiOBHm_Chr3g0447601
rosa_laevigata	RLG00000018425 RLG00000025985
rosa_multiflora	Rmu_sc0001814.1_g000009 Rmu_sc0003550.1_g000005
rosa_roxburghii	Rroxscaffold_2G00125130 Rroxscaffold_6G00425810
rosa_rugosa	Rorug02G0219200 Rorug02G0603400
rosa_samantha	Rh2AG276300 Rh2BG288100 Rh2DG283200 Rh2DG302300 Rh3AG003600 Rh3BG003100 Rh3CG003400 Rh3DG003400
rosa_wichuraiana	Rw2G021990 Rw3G000250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1000
Acc36I	ACCTGC	1 cut(s)	1000
AccB1I	GGYRCC	1 cut(s)	1236
AccI	GTMKAC	1 cut(s)	638
AccII	CGCG	1 cut(s)	1215
AciI	CCGC	2 cut(s)	96, 148
AclI	AACGTT	1 cut(s)	194
AclWI	GGATC	9 cut(s)	221, 234, 534, 776, 841, 950, 1049, 1218, 1247
AcoI	YGGCCR	1 cut(s)	218
AcsI	RAATTY	3 cut(s)	341, 774, 828
AcuI	CTGAAG	1 cut(s)	995
AcyI	GRCGYC	1 cut(s)	1237
AfaI	GTAC	3 cut(s)	203, 291, 431
AfiI	CCNNNNNNNGG	2 cut(s)	501, 807
AgsI	TTSAA	4 cut(s)	199, 248, 762, 1001
AjnI	CCWGG	1 cut(s)	498
AluBI	AGCT	7 cut(s)	40, 53, 61, 382, 393, 1118, 1148
AluI	AGCT	7 cut(s)	40, 53, 61, 382, 393, 1118, 1148
Alw21I	GWGCWC	1 cut(s)	55
Alw26I	GTCTC	1 cut(s)	1095
AlwI	GGATC	9 cut(s)	221, 234, 534, 776, 841, 950, 1049, 1218, 1247
AlwNI	CAGNNNCTG	1 cut(s)	64
Ama87I	CYCGRG	2 cut(s)	941, 1149
AoxI	GGCC	3 cut(s)	218, 578, 869
ApeKI	GCWGC	1 cut(s)	58
ApoI	RAATTY	3 cut(s)	341, 774, 828
AseI	ATTAAT	1 cut(s)	723
Asp700I	GAANNNNTTC	1 cut(s)	410
AspLEI	GCGC	2 cut(s)	882, 1239
AspS9I	GGNCC	2 cut(s)	446, 869
AsuC2I	CCSGG	1 cut(s)	222
AsuHPI	GGTGA	3 cut(s)	431, 1024, 1199
AsuII	TTCGAA	2 cut(s)	117, 129
AvaI	CYCGRG	2 cut(s)	941, 1149
AvaII	GGWCC	1 cut(s)	446
BamHI	GGATCC	1 cut(s)	226
BanI	GGYRCC	1 cut(s)	1236
BanII	GRGCYC	2 cut(s)	55, 801
BarI	GAAGNNNNNNTAC	2 cut(s)	622, 654
BbsI	GAAGAC	2 cut(s)	810, 926
Bbv12I	GWGCWC	1 cut(s)	55
BbvI	GCAGC	1 cut(s)	70
BccI	CCATC	2 cut(s)	128, 775
BceAI	ACGGC	1 cut(s)	1224
BciT130I	CCWGG	1 cut(s)	500
BcnI	CCSGG	1 cut(s)	222
BcoDI	GTCTC	1 cut(s)	1095
BfaI	CTAG	2 cut(s)	320, 390
BfmI	CTRYAG	2 cut(s)	56, 207
BfoI	RGCGCY	1 cut(s)	1240
BfuAI	ACCTGC	1 cut(s)	1000
BisI	GCNGC	2 cut(s)	59, 148
BlsI	GCNGC	2 cut(s)	60, 149
Bme1390I	CCNGG	2 cut(s)	222, 500
Bme18I	GGWCC	1 cut(s)	446
BmeT110I	CYCGRG	2 cut(s)	941, 1149
BmgT120I	GGNCC	2 cut(s)	446, 869
BmiI	GGNNCC	2 cut(s)	228, 1238
BmrFI	CCNGG	2 cut(s)	222, 500
BmrI	ACTGGG	2 cut(s)	712, 817
BmsI	GCATC	2 cut(s)	250, 574
BmuI	ACTGGG	2 cut(s)	712, 817
BpiI	GAAGAC	2 cut(s)	810, 926
BplI	GAGNNNNNCTC	2 cut(s)	1132, 1164
Bpu14I	TTCGAA	2 cut(s)	117, 129
BpuMI	CCSGG	1 cut(s)	222
BsaHI	GRCGYC	1 cut(s)	1237
BsaJI	CCNNGG	5 cut(s)	221, 499, 872, 926, 1058
BsaWI	WCCGGW	1 cut(s)	745
BsaXI	ACNNNNNCTCC	2 cut(s)	1225, 1255
Bsc4I	CCNNNNNNNGG	2 cut(s)	501, 807
Bse1I	ACTGG	4 cut(s)	170, 328, 707, 812
BseBI	CCWGG	1 cut(s)	500
BseDI	CCNNGG	5 cut(s)	221, 499, 872, 926, 1058
BseGI	GGATG	4 cut(s)	682, 712, 817, 1276
BseLI	CCNNNNNNNGG	2 cut(s)	501, 807
BseMII	CTCAG	2 cut(s)	285, 1098
BseNI	ACTGG	4 cut(s)	170, 328, 707, 812
BseRI	GAGGAG	1 cut(s)	1175
BseXI	GCAGC	1 cut(s)	70
BseYI	CCCAGC	1 cut(s)	150
Bsh1236I	CGCG	1 cut(s)	1215
BshFI	GGCC	3 cut(s)	220, 580, 871
BshNI	GGYRCC	1 cut(s)	1236
BsiHKAI	GWGCWC	1 cut(s)	55
BsiHKCI	CYCGRG	2 cut(s)	941, 1149
BsiSI	CCGG	2 cut(s)	221, 746
BslI	CCNNNNNNNGG	2 cut(s)	501, 807
BsmAI	GTCTC	1 cut(s)	1095
BsmBI	CGTCTC	1 cut(s)	1095
BsmI	GAATGC	1 cut(s)	243
BsnI	GGCC	3 cut(s)	220, 580, 871
BsoBI	CYCGRG	2 cut(s)	941, 1149
Bsp119I	TTCGAA	2 cut(s)	117, 129
Bsp1286I	GDGCHC	2 cut(s)	55, 801
Bsp143I	GATC	8 cut(s)	226, 526, 781, 846, 955, 1054, 1210, 1252
Bsp19I	CCATGG	2 cut(s)	872, 926
Bsp68I	TCGCGA	1 cut(s)	1215
BspACI	CCGC	2 cut(s)	96, 148
BspANI	GGCC	3 cut(s)	220, 580, 871
BspCNI	CTCAG	2 cut(s)	286, 1099
BspFNI	CGCG	1 cut(s)	1215
BspLI	GGNNCC	2 cut(s)	228, 1238
BspMAI	CTGCAG	2 cut(s)	60, 211
BspMI	ACCTGC	1 cut(s)	1000
BspPI	GGATC	9 cut(s)	221, 234, 534, 776, 841, 950, 1049, 1218, 1247
BspT104I	TTCGAA	2 cut(s)	117, 129
BspT107I	GGYRCC	1 cut(s)	1236
BsrI	ACTGG	4 cut(s)	170, 328, 707, 812
BssECI	CCNNGG	5 cut(s)	221, 499, 872, 926, 1058
BssMI	GATC	8 cut(s)	226, 526, 781, 846, 955, 1054, 1210, 1252
BssNAI	GTATAC	1 cut(s)	639
BssNI	GRCGYC	1 cut(s)	1237
BssT1I	CCWWGG	2 cut(s)	872, 926
Bst1107I	GTATAC	1 cut(s)	639
Bst2UI	CCWGG	1 cut(s)	500
Bst4CI	ACNGT	3 cut(s)	434, 1062, 1290
Bst6I	CTCTTC	4 cut(s)	813, 1077, 1152, 1193
BstACI	GRCGYC	1 cut(s)	1237
BstAPI	GCANNNNNTGC	1 cut(s)	64
BstBI	TTCGAA	2 cut(s)	117, 129
BstC8I	GCNNGC	1 cut(s)	696
BstDEI	CTNAG	3 cut(s)	294, 575, 1107
BstDSI	CCRYGG	3 cut(s)	872, 926, 1058
BstF5I	GGATG	4 cut(s)	682, 712, 817, 1276
BstFNI	CGCG	1 cut(s)	1215
BstH2I	RGCGCY	1 cut(s)	1240
BstHHI	GCGC	2 cut(s)	882, 1239
BstKTI	GATC	8 cut(s)	229, 529, 784, 849, 958, 1057, 1213, 1255
BstMAI	GTCTC	1 cut(s)	1095
BstMBI	GATC	8 cut(s)	226, 526, 781, 846, 955, 1054, 1210, 1252
BstMWI	GCNNNNNNNGC	5 cut(s)	64, 281, 877, 1172, 1221
BstNI	CCWGG	1 cut(s)	500
BstNSI	RCATGY	1 cut(s)	637
BstSCI	CCNGG	2 cut(s)	220, 498
BstSFI	CTRYAG	2 cut(s)	56, 207
BstUI	CGCG	1 cut(s)	1215
BstV1I	GCAGC	1 cut(s)	70
BstV2I	GAAGAC	2 cut(s)	810, 926
BstX2I	RGATCY	3 cut(s)	226, 526, 846
BstXI	CCANNNNNNTGG	1 cut(s)	702
BstYI	RGATCY	3 cut(s)	226, 526, 846
BstZ17I	GTATAC	1 cut(s)	639
BsuRI	GGCC	3 cut(s)	220, 580, 871
BtgI	CCRYGG	3 cut(s)	872, 926, 1058
BtsCI	GGATG	4 cut(s)	682, 712, 817, 1276
BtsI	GCAGTG	1 cut(s)	204
BtsIMutI	CAGTG	1 cut(s)	204
BtuMI	TCGCGA	1 cut(s)	1215
BveI	ACCTGC	1 cut(s)	1000
Cac8I	GCNNGC	1 cut(s)	696
CaiI	CAGNNNCTG	1 cut(s)	64
CfoI	GCGC	2 cut(s)	882, 1239
Cfr13I	GGNCC	2 cut(s)	446, 869
CseI	GACGC	2 cut(s)	586, 1112
Csp6I	GTAC	3 cut(s)	202, 290, 430
CviAII	CATG	8 cut(s)	439, 482, 634, 873, 927, 1250, 1259, 1280
CviQI	GTAC	3 cut(s)	202, 290, 430
DdeI	CTNAG	3 cut(s)	294, 575, 1107
DinI	GGCGCC	1 cut(s)	1238
DpnI	GATC	8 cut(s)	228, 528, 783, 848, 957, 1056, 1212, 1254
DpnII	GATC	8 cut(s)	226, 526, 781, 846, 955, 1054, 1210, 1252
EaeI	YGGCCR	1 cut(s)	218
Eam1104I	CTCTTC	4 cut(s)	813, 1077, 1152, 1193
EarI	CTCTTC	4 cut(s)	813, 1077, 1152, 1193
EciI	GGCGGA	1 cut(s)	85
Ecl136II	GAGCTC	1 cut(s)	53
Eco130I	CCWWGG	2 cut(s)	872, 926
Eco147I	AGGCCT	1 cut(s)	580
Eco24I	GRGCYC	2 cut(s)	55, 801
Eco32I	GATATC	1 cut(s)	652
Eco47I	GGWCC	1 cut(s)	446
Eco53kI	GAGCTC	1 cut(s)	53
Eco57I	CTGAAG	1 cut(s)	995
Eco88I	CYCGRG	2 cut(s)	941, 1149
EcoICRI	GAGCTC	1 cut(s)	53
EcoRII	CCWGG	1 cut(s)	498
EcoRV	GATATC	1 cut(s)	652
EcoT14I	CCWWGG	2 cut(s)	872, 926
EcoT38I	GRGCYC	2 cut(s)	55, 801
EgeI	GGCGCC	1 cut(s)	1238
EheI	GGCGCC	1 cut(s)	1238
ErhI	CCWWGG	2 cut(s)	872, 926
Esp3I	CGTCTC	1 cut(s)	1095
FaeI	CATG	8 cut(s)	442, 485, 637, 876, 930, 1253, 1262, 1283
FatI	CATG	8 cut(s)	438, 481, 633, 872, 926, 1249, 1258, 1279
FblI	GTMKAC	1 cut(s)	638
Fnu4HI	GCNGC	2 cut(s)	59, 148
FokI	GGATG	4 cut(s)	689, 719, 824, 1283
FriOI	GRGCYC	2 cut(s)	55, 801
Fsp4HI	GCNGC	2 cut(s)	59, 148
FspBI	CTAG	2 cut(s)	320, 390
GlaI	GCGC	2 cut(s)	881, 1238
GluI	GCNGC	2 cut(s)	59, 148
GsaI	CCCAGC	1 cut(s)	154
HaeII	RGCGCY	1 cut(s)	1240
HaeIII	GGCC	3 cut(s)	220, 580, 871
HapII	CCGG	2 cut(s)	221, 746
HgaI	GACGC	2 cut(s)	586, 1112
HhaI	GCGC	2 cut(s)	882, 1239
Hin1I	GRCGYC	1 cut(s)	1237
Hin1II	CATG	8 cut(s)	442, 485, 637, 876, 930, 1253, 1262, 1283
Hin6I	GCGC	2 cut(s)	880, 1237
HinP1I	GCGC	2 cut(s)	880, 1237
HincII	GTYRAC	1 cut(s)	552
HindII	GTYRAC	1 cut(s)	552
HindIII	AAGCTT	3 cut(s)	38, 380, 1116
HinfI	GANTC	4 cut(s)	126, 623, 758, 922
HpaI	GTTAAC	1 cut(s)	552
HpaII	CCGG	2 cut(s)	221, 746
HphI	GGTGA	3 cut(s)	431, 1024, 1199
Hpy166II	GTNNAC	4 cut(s)	204, 552, 639, 682
Hpy188I	TCNGA	4 cut(s)	106, 163, 405, 772
Hpy188III	TCNNGA	9 cut(s)	230, 614, 627, 779, 892, 959, 1151, 1214, 1268
Hpy8I	GTNNAC	4 cut(s)	204, 552, 639, 682
HpyAV	CCTTC	5 cut(s)	173, 665, 1009, 1178, 1239
HpyCH4III	ACNGT	3 cut(s)	434, 1062, 1290
HpyCH4IV	ACGT	1 cut(s)	194
HpyCH4V	TGCA	4 cut(s)	58, 209, 587, 1067
HpyF10VI	GCNNNNNNNGC	5 cut(s)	64, 281, 877, 1172, 1221
HpyF3I	CTNAG	3 cut(s)	294, 575, 1107
HpySE526I	ACGT	1 cut(s)	194
Hsp92I	GRCGYC	1 cut(s)	1237
Hsp92II	CATG	8 cut(s)	442, 485, 637, 876, 930, 1253, 1262, 1283
HspAI	GCGC	2 cut(s)	880, 1237
KasI	GGCGCC	1 cut(s)	1236
KspAI	GTTAAC	1 cut(s)	552
Kzo9I	GATC	8 cut(s)	226, 526, 781, 846, 955, 1054, 1210, 1252
Lsp1109I	GCAGC	1 cut(s)	70
LweI	GCATC	2 cut(s)	250, 574
MaeI	CTAG	2 cut(s)	320, 390
MaeII	ACGT	1 cut(s)	194
MaeIII	GTNAC	1 cut(s)	371
MalI	GATC	8 cut(s)	228, 528, 783, 848, 957, 1056, 1212, 1254
MboI	GATC	8 cut(s)	226, 526, 781, 846, 955, 1054, 1210, 1252
MflI	RGATCY	3 cut(s)	226, 526, 846
MhlI	GDGCHC	2 cut(s)	55, 801
MluCI	AATT	7 cut(s)	341, 508, 762, 774, 828, 981, 1044
Mly113I	GGCGCC	1 cut(s)	1237
MlyI	GAGTC	1 cut(s)	916
MroXI	GAANNNNTTC	1 cut(s)	410
MseI	TTAA	5 cut(s)	339, 551, 723, 984, 1043
MspA1I	CMGCKG	1 cut(s)	150
MspI	CCGG	2 cut(s)	221, 746
MspR9I	CCNGG	2 cut(s)	222, 500
Mva1269I	GAATGC	1 cut(s)	243
MvaI	CCWGG	1 cut(s)	500
MvnI	CGCG	1 cut(s)	1215
MwoI	GCNNNNNNNGC	5 cut(s)	64, 281, 877, 1172, 1221
NarI	GGCGCC	1 cut(s)	1237
NciI	CCSGG	1 cut(s)	222
NcoI	CCATGG	2 cut(s)	872, 926
NdeII	GATC	8 cut(s)	226, 526, 781, 846, 955, 1054, 1210, 1252
NlaIII	CATG	8 cut(s)	442, 485, 637, 876, 930, 1253, 1262, 1283
NlaIV	GGNNCC	2 cut(s)	228, 1238
NruI	TCGCGA	1 cut(s)	1215
NspI	RCATGY	1 cut(s)	637
NspV	TTCGAA	2 cut(s)	117, 129
PaeR7I	CTCGAG	1 cut(s)	1149
PaqCI	CACCTGC	1 cut(s)	1000
PceI	AGGCCT	1 cut(s)	580
PctI	GAATGC	1 cut(s)	243
PdmI	GAANNNNTTC	1 cut(s)	410
PfeI	GAWTC	3 cut(s)	126, 623, 758
PkrI	GCNGC	2 cut(s)	60, 149
PleI	GAGTC	1 cut(s)	916
PluTI	GGCGCC	1 cut(s)	1240
PpsI	GAGTC	1 cut(s)	916
PshBI	ATTAAT	1 cut(s)	723
Psp124BI	GAGCTC	1 cut(s)	55
Psp1406I	AACGTT	1 cut(s)	194
Psp6I	CCWGG	1 cut(s)	498
PspFI	CCCAGC	1 cut(s)	150
PspGI	CCWGG	1 cut(s)	498
PspN4I	GGNNCC	2 cut(s)	228, 1238
PspPI	GGNCC	2 cut(s)	446, 869
PstI	CTGCAG	2 cut(s)	60, 211
PstNI	CAGNNNCTG	1 cut(s)	64
PsuI	RGATCY	3 cut(s)	226, 526, 846
RruI	TCGCGA	1 cut(s)	1215
RsaI	GTAC	3 cut(s)	203, 291, 431
RsaNI	GTAC	3 cut(s)	202, 290, 430
SacI	GAGCTC	1 cut(s)	55
SaqAI	TTAA	5 cut(s)	339, 551, 723, 984, 1043
SatI	GCNGC	2 cut(s)	59, 148
Sau3AI	GATC	8 cut(s)	226, 526, 781, 846, 955, 1054, 1210, 1252
Sau96I	GGNCC	2 cut(s)	446, 869
SchI	GAGTC	1 cut(s)	916
ScrFI	CCNGG	2 cut(s)	222, 500
SduI	GDGCHC	2 cut(s)	55, 801
SfaNI	GCATC	2 cut(s)	250, 574
SfcI	CTRYAG	2 cut(s)	56, 207
SfoI	GGCGCC	1 cut(s)	1238
Sfr274I	CTCGAG	1 cut(s)	1149
SfuI	TTCGAA	2 cut(s)	117, 129
SinI	GGWCC	1 cut(s)	446
SlaI	CTCGAG	1 cut(s)	1149
SmlI	CTYRAG	1 cut(s)	1149
SmoI	CTYRAG	1 cut(s)	1149
Sse9I	AATT	7 cut(s)	341, 508, 762, 774, 828, 981, 1044
SseBI	AGGCCT	1 cut(s)	580
SsiI	CCGC	2 cut(s)	96, 148
SspDI	GGCGCC	1 cut(s)	1236
SspMI	CTAG	2 cut(s)	320, 390
SstI	GAGCTC	1 cut(s)	55
StuI	AGGCCT	1 cut(s)	580
StyD4I	CCNGG	2 cut(s)	220, 498
StyI	CCWWGG	2 cut(s)	872, 926
TaaI	ACNGT	3 cut(s)	434, 1062, 1290
TaiI	ACGT	1 cut(s)	197
TaqI	TCGA	7 cut(s)	49, 117, 129, 335, 613, 1032, 1150
TasI	AATT	7 cut(s)	341, 508, 762, 774, 828, 981, 1044
TatI	WGTACW	1 cut(s)	201
TauI	GCSGC	1 cut(s)	150
TfiI	GAWTC	3 cut(s)	126, 623, 758
Tru1I	TTAA	5 cut(s)	339, 551, 723, 984, 1043
Tru9I	TTAA	5 cut(s)	339, 551, 723, 984, 1043
TscAI	CASTG	1 cut(s)	211
TseI	GCWGC	1 cut(s)	58
TspDTI	ATGAA	3 cut(s)	50, 427, 750
TspGWI	ACGGA	1 cut(s)	80
TspRI	CASTG	1 cut(s)	211
VpaK11BI	GGWCC	1 cut(s)	446
VspI	ATTAAT	1 cut(s)	723
XapI	RAATTY	3 cut(s)	341, 774, 828
XceI	RCATGY	1 cut(s)	637
XhoI	CTCGAG	1 cut(s)	1149
XmiI	GTMKAC	1 cut(s)	638
XmnI	GAANNNNTTC	1 cut(s)	410
XspI	CTAG	2 cut(s)	320, 390

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.