Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0001814.1

Orthogroup ID

OG0002902

Physical Location & Seq

Genomic Coordinates Reverse (-)

53061 .. 59500

Gene Structure 6440 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0001814.1_g000009.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1263 bp

atgaagcttctcgaatctcttcttcttcttcttcttctggttccgcttctatgcctccacttccgtctcgccgtctccgaaatcttcttcgaagagcgatttgaagatgggtggaagagccggtgggtgttgtcggactggaaaagggccgaaggaaaagcaggaacgtttaagtacactgctgggaaatggtctggagatcctgatgataaaggcattcagacatataatgatgccaagcattatgccatatctgcaaaaattcctgagttcagcaacaaaaacagaactctagtggtccaatattcgattaaatttgagcaagacattgaatgtggtggcggttacatcaagcttctatctggctatgtgaatcagaaaaagttcggcggtgataccccatacagtttcatgtttggaccagatatgtgtggctcacagaccaaaaaactccatcttatactttcttaccaggggcagaactatcccataaaaaaggatcttcaatgtgaaacagacaagtttactcatttctatacatttattcttagacctgatgcaacttacagtgtccttgttgataaccgagaaagagattctggaagcatgtatactgattgggatatccttcctccacgaaaaatcaaggatgtcaatgcaagaaagccatcagactgggatgacaaagagtacattgaagaccgtaaagccgtcaaaccagagggatatgattcagttccatctgaaattcctgatccaaaggccaaagagcccgaggactgggacgaggaagagaacggattgtggagaccaccaaagatcccaaatccagcttataagggcccatggaagcgcaagaaaatcaagaaccccaactacaaggggaagtggaagactccgtggattgataacccagagtttgaggatgatcctgacctttatgtgctgaagccaataaaatatgtgggcattgaagtttggcaggtaaaagctggttcagttttcgacaacattttgatctgtgatgatccacagtatgcaaaacaagtggtagaagagatattttccaatagagaggctgaaaaagaagcttttgaggaagcagagaaggagagaagagcacgagaagcagaggatgctagaagagcaagagaggaaggtgaaagaaagaagtgggagagggatcgtcaatatggagataagaggcgccgtagaaggcatgatccccgggattatttggatgattaccatgatgaactatga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006457 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009812 GO:0009987 GO:0010204 GO:0012501 GO:0012505 GO:0016020 GO:0023052 GO:0031347 GO:0031349 GO:0031984 GO:0033554 GO:0034050 GO:0042175 GO:0042440 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046283 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071704 GO:0080134 GO:0098542 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko04141 ko04145 ko04612 ko05142 ko05166 map04141 map04145 map04612 map05142 map05166

Pfam Domains

Protein Families

Calreticulin

Protein Analysis

420

Amino Acids

49.57

Weight (kDa)

5.91

Isoelectric Point (pI)

46.53

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002902)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08450 AT1G08450 AT1G08450
fragaria_vesca	FvH4_1g23470 FvH4_1g23470 FvH4_1g23470 FvH4_1g23470 FvH4_1g23470 FvH4_6g00230 FvH4_6g00230 FvH4_6g00230
malus_domestica	MD04G1045300.v1.1 MD04G1247000.v1.1 MD12G1263200.v1.1
prunus_persica	Prupe.1G198500_v2.0.a1 Prupe.6G363200_v2.0.a1
pyrus_communis	pycom04g21770 pycom12g24070
rosa_chinensis	RchiOBHm_Chr2g0118841 RchiOBHm_Chr3g0447601
rosa_laevigata	RLG00000018425 RLG00000025985
rosa_multiflora	Rmu_sc0001814.1_g000009 Rmu_sc0003550.1_g000005
rosa_roxburghii	Rroxscaffold_2G00125130 Rroxscaffold_6G00425810
rosa_rugosa	Rorug02G0219200 Rorug02G0603400
rosa_samantha	Rh2AG276300 Rh2BG288100 Rh2DG283200 Rh2DG302300 Rh3AG003600 Rh3BG003100 Rh3CG003400 Rh3DG003400
rosa_wichuraiana	Rw2G021990 Rw3G000250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	837
Acc36I	ACCTGC	1 cut(s)	973
AccB1I	GGYRCC	1 cut(s)	1206
AccI	GTMKAC	1 cut(s)	611
AciI	CCGC	3 cut(s)	44, 342, 390
AclI	AACGTT	1 cut(s)	167
AclWI	GGATC	8 cut(s)	194, 507, 749, 814, 923, 1022, 1191, 1217
AcsI	RAATTY	3 cut(s)	261, 314, 747
AcuI	CTGAAG	1 cut(s)	968
AcyI	GRCGYC	1 cut(s)	1207
AfaI	GTAC	2 cut(s)	176, 692
AfiI	CCNNNNNNNGG	1 cut(s)	780
AgsI	TTSAA	5 cut(s)	104, 332, 506, 698, 974
AjnI	CCWGG	1 cut(s)	471
AluBI	AGCT	5 cut(s)	7, 355, 833, 992, 1091
AluI	AGCT	5 cut(s)	7, 355, 833, 992, 1091
Alw21I	GWGCWC	1 cut(s)	1123
Alw26I	GTCTC	3 cut(s)	71, 79, 802
AlwI	GGATC	8 cut(s)	194, 507, 749, 814, 923, 1022, 1191, 1217
Ama87I	CYCGRG	2 cut(s)	773, 1227
AoxI	GGCC	3 cut(s)	147, 762, 841
ApaI	GGGCCC	1 cut(s)	845
ApoI	RAATTY	3 cut(s)	261, 314, 747
Asp700I	GAANNNNTTC	2 cut(s)	18, 383
AspLEI	GCGC	2 cut(s)	855, 1209
AspS9I	GGNCC	5 cut(s)	147, 298, 419, 841, 842
AsuC2I	CCSGG	2 cut(s)	1228, 1229
AsuHPI	GGTGA	2 cut(s)	404, 1172
AsuII	TTCGAA	1 cut(s)	90
AvaI	CYCGRG	2 cut(s)	773, 1227
AvaII	GGWCC	2 cut(s)	298, 419
BaeGI	GKGCMC	1 cut(s)	845
BanI	GGYRCC	1 cut(s)	1206
BanII	GRGCYC	2 cut(s)	774, 845
BarI	GAAGNNNNNNTAC	2 cut(s)	595, 627
BauI	CACGAG	1 cut(s)	1122
BbsI	GAAGAC	2 cut(s)	705, 899
Bbv12I	GWGCWC	1 cut(s)	1123
BccI	CCATC	4 cut(s)	101, 462, 676, 748
BceAI	ACGGC	3 cut(s)	56, 695, 1194
BciT130I	CCWGG	1 cut(s)	473
BcnI	CCSGG	2 cut(s)	1228, 1229
BcoDI	GTCTC	3 cut(s)	71, 79, 802
BfaI	CTAG	2 cut(s)	293, 1140
BfoI	RGCGCY	1 cut(s)	1210
BfuAI	ACCTGC	1 cut(s)	973
Bme1390I	CCNGG	3 cut(s)	473, 1228, 1229
Bme18I	GGWCC	2 cut(s)	298, 419
BmeT110I	CYCGRG	2 cut(s)	773, 1227
BmgT120I	GGNCC	5 cut(s)	147, 298, 419, 841, 842
BmiI	GGNNCC	3 cut(s)	42, 843, 1208
BmrFI	CCNGG	3 cut(s)	473, 1228, 1229
BmrI	ACTGGG	2 cut(s)	685, 790
BmsI	GCATC	3 cut(s)	223, 547, 1126
BmuI	ACTGGG	2 cut(s)	685, 790
BpiI	GAAGAC	2 cut(s)	705, 899
BpmI	CTGGAG	1 cut(s)	216
Bpu14I	TTCGAA	1 cut(s)	90
BpuMI	CCSGG	2 cut(s)	1228, 1229
BsaHI	GRCGYC	1 cut(s)	1207
BsaI	GGTCTC	1 cut(s)	802
BsaJI	CCNNGG	6 cut(s)	472, 774, 845, 899, 1226, 1227
Bsc4I	CCNNNNNNNGG	1 cut(s)	780
Bse118I	RCCGGY	1 cut(s)	120
Bse1I	ACTGG	3 cut(s)	143, 680, 785
BseBI	CCWGG	1 cut(s)	473
BseDI	CCNNGG	6 cut(s)	472, 774, 845, 899, 1226, 1227
BseGI	GGATG	5 cut(s)	655, 685, 931, 1141, 1246
BseLI	CCNNNNNNNGG	1 cut(s)	780
BseMII	CTCAG	1 cut(s)	258
BseNI	ACTGG	3 cut(s)	143, 680, 785
BseSI	GKGCMC	1 cut(s)	845
BseYI	CCCAGC	1 cut(s)	182
BshFI	GGCC	3 cut(s)	149, 764, 843
BshNI	GGYRCC	1 cut(s)	1206
BsiHKAI	GWGCWC	1 cut(s)	1123
BsiHKCI	CYCGRG	2 cut(s)	773, 1227
BsiSI	CCGG	2 cut(s)	121, 1228
BslFI	GGGAC	1 cut(s)	797
BslI	CCNNNNNNNGG	1 cut(s)	780
BsmAI	GTCTC	3 cut(s)	71, 79, 802
BsmBI	CGTCTC	2 cut(s)	71, 79
BsmFI	GGGAC	1 cut(s)	797
BsmI	GAATGC	1 cut(s)	216
BsnI	GGCC	3 cut(s)	149, 764, 843
Bso31I	GGTCTC	1 cut(s)	802
BsoBI	CYCGRG	2 cut(s)	773, 1227
Bsp119I	TTCGAA	1 cut(s)	90
Bsp120I	GGGCCC	1 cut(s)	841
Bsp1286I	GDGCHC	3 cut(s)	774, 845, 1123
Bsp143I	GATC	9 cut(s)	199, 499, 754, 819, 928, 1017, 1027, 1183, 1222
Bsp19I	CCATGG	1 cut(s)	845
BspACI	CCGC	3 cut(s)	44, 342, 390
BspANI	GGCC	3 cut(s)	149, 764, 843
BspCNI	CTCAG	1 cut(s)	259
BspLI	GGNNCC	3 cut(s)	42, 843, 1208
BspMI	ACCTGC	1 cut(s)	973
BspPI	GGATC	8 cut(s)	194, 507, 749, 814, 923, 1022, 1191, 1217
BspQI	GCTCTTC	4 cut(s)	87, 110, 1111, 1138
BspT104I	TTCGAA	1 cut(s)	90
BspT107I	GGYRCC	1 cut(s)	1206
BspTNI	GGTCTC	1 cut(s)	802
BsrFI	RCCGGY	1 cut(s)	120
BsrI	ACTGG	3 cut(s)	143, 680, 785
BssAI	RCCGGY	1 cut(s)	120
BssECI	CCNNGG	6 cut(s)	472, 774, 845, 899, 1226, 1227
BssMI	GATC	9 cut(s)	199, 499, 754, 819, 928, 1017, 1027, 1183, 1222
BssNAI	GTATAC	1 cut(s)	612
BssNI	GRCGYC	1 cut(s)	1207
BssSI	CACGAG	1 cut(s)	1122
BssT1I	CCWWGG	1 cut(s)	845
Bst1107I	GTATAC	1 cut(s)	612
Bst2BI	CACGAG	1 cut(s)	1122
Bst2UI	CCWGG	1 cut(s)	473
Bst4CI	ACNGT	4 cut(s)	407, 569, 704, 1035
Bst6I	CTCTTC	7 cut(s)	24, 87, 110, 786, 1050, 1111, 1138
BstACI	GRCGYC	1 cut(s)	1207
BstAPI	GCANNNNNTGC	1 cut(s)	1136
BstBI	TTCGAA	1 cut(s)	90
BstDEI	CTNAG	2 cut(s)	267, 548
BstDSI	CCRYGG	2 cut(s)	845, 899
BstF5I	GGATG	5 cut(s)	655, 685, 931, 1141, 1246
BstH2I	RGCGCY	1 cut(s)	1210
BstHHI	GCGC	2 cut(s)	855, 1209
BstKTI	GATC	9 cut(s)	202, 502, 757, 822, 931, 1020, 1030, 1186, 1225
BstMAI	GTCTC	3 cut(s)	71, 79, 802
BstMBI	GATC	9 cut(s)	199, 499, 754, 819, 928, 1017, 1027, 1183, 1222
BstMWI	GCNNNNNNNGC	4 cut(s)	254, 1127, 1136, 1145
BstNI	CCWGG	1 cut(s)	473
BstNSI	RCATGY	1 cut(s)	610
BstSCI	CCNGG	3 cut(s)	471, 1226, 1227
BstSLI	GKGCMC	1 cut(s)	845
BstV2I	GAAGAC	2 cut(s)	705, 899
BstX2I	RGATCY	3 cut(s)	199, 499, 819
BstXI	CCANNNNNNTGG	1 cut(s)	675
BstYI	RGATCY	3 cut(s)	199, 499, 819
BstZ17I	GTATAC	1 cut(s)	612
BsuRI	GGCC	3 cut(s)	149, 764, 843
BtgI	CCRYGG	2 cut(s)	845, 899
BtsCI	GGATG	5 cut(s)	655, 685, 931, 1141, 1246
BtsI	GCAGTG	1 cut(s)	177
BtsIMutI	CAGTG	2 cut(s)	177, 574
BveI	ACCTGC	1 cut(s)	973
CfoI	GCGC	2 cut(s)	855, 1209
Cfr10I	RCCGGY	1 cut(s)	120
Cfr13I	GGNCC	5 cut(s)	147, 298, 419, 841, 842
Cfr9I	CCCGGG	1 cut(s)	1227
Csp6I	GTAC	2 cut(s)	175, 691
CspCI	CAANNNNNGTGG	2 cut(s)	1029, 1064
CviAII	CATG	5 cut(s)	412, 607, 846, 1220, 1250
CviQI	GTAC	2 cut(s)	175, 691
DdeI	CTNAG	2 cut(s)	267, 548
DinI	GGCGCC	1 cut(s)	1208
DpnI	GATC	9 cut(s)	201, 501, 756, 821, 930, 1019, 1029, 1185, 1224
DpnII	GATC	9 cut(s)	199, 499, 754, 819, 928, 1017, 1027, 1183, 1222
Eam1104I	CTCTTC	7 cut(s)	24, 87, 110, 786, 1050, 1111, 1138
EarI	CTCTTC	7 cut(s)	24, 87, 110, 786, 1050, 1111, 1138
Eco130I	CCWWGG	1 cut(s)	845
Eco24I	GRGCYC	2 cut(s)	774, 845
Eco31I	GGTCTC	1 cut(s)	802
Eco32I	GATATC	1 cut(s)	625
Eco47I	GGWCC	2 cut(s)	298, 419
Eco57I	CTGAAG	1 cut(s)	968
Eco88I	CYCGRG	2 cut(s)	773, 1227
EcoO109I	RGGNCCY	1 cut(s)	841
EcoRII	CCWGG	1 cut(s)	471
EcoRV	GATATC	1 cut(s)	625
EcoT14I	CCWWGG	1 cut(s)	845
EcoT38I	GRGCYC	2 cut(s)	774, 845
EgeI	GGCGCC	1 cut(s)	1208
EheI	GGCGCC	1 cut(s)	1208
ErhI	CCWWGG	1 cut(s)	845
Esp3I	CGTCTC	2 cut(s)	71, 79
FaeI	CATG	5 cut(s)	415, 610, 849, 1223, 1253
FaqI	GGGAC	1 cut(s)	797
FatI	CATG	5 cut(s)	411, 606, 845, 1219, 1249
FblI	GTMKAC	1 cut(s)	611
FokI	GGATG	5 cut(s)	662, 692, 938, 1148, 1253
FriOI	GRGCYC	2 cut(s)	774, 845
FspBI	CTAG	2 cut(s)	293, 1140
GlaI	GCGC	2 cut(s)	854, 1208
GsaI	CCCAGC	1 cut(s)	186
GsuI	CTGGAG	1 cut(s)	216
HaeII	RGCGCY	1 cut(s)	1210
HaeIII	GGCC	3 cut(s)	149, 764, 843
HapII	CCGG	2 cut(s)	121, 1228
HhaI	GCGC	2 cut(s)	855, 1209
Hin1I	GRCGYC	1 cut(s)	1207
Hin1II	CATG	5 cut(s)	415, 610, 849, 1223, 1253
Hin6I	GCGC	2 cut(s)	853, 1207
HinP1I	GCGC	2 cut(s)	853, 1207
HindIII	AAGCTT	3 cut(s)	5, 353, 1089
HinfI	GANTC	5 cut(s)	14, 373, 596, 731, 895
HpaII	CCGG	2 cut(s)	121, 1228
HphI	GGTGA	2 cut(s)	404, 1172
Hpy166II	GTNNAC	3 cut(s)	177, 525, 612
Hpy188I	TCNGA	6 cut(s)	79, 136, 222, 378, 673, 745
Hpy188III	TCNNGA	8 cut(s)	11, 195, 203, 266, 600, 752, 865, 932
Hpy8I	GTNNAC	3 cut(s)	177, 525, 612
HpyAV	CCTTC	5 cut(s)	146, 638, 1102, 1151, 1209
HpyCH4III	ACNGT	4 cut(s)	407, 569, 704, 1035
HpyCH4IV	ACGT	1 cut(s)	167
HpyCH4V	TGCA	4 cut(s)	257, 560, 659, 1040
HpyF10VI	GCNNNNNNNGC	4 cut(s)	254, 1127, 1136, 1145
HpyF3I	CTNAG	2 cut(s)	267, 548
HpySE526I	ACGT	1 cut(s)	167
Hsp92I	GRCGYC	1 cut(s)	1207
Hsp92II	CATG	5 cut(s)	415, 610, 849, 1223, 1253
HspAI	GCGC	2 cut(s)	853, 1207
KasI	GGCGCC	1 cut(s)	1206
Kzo9I	GATC	9 cut(s)	199, 499, 754, 819, 928, 1017, 1027, 1183, 1222
LguI	GCTCTTC	4 cut(s)	87, 110, 1111, 1138
LweI	GCATC	3 cut(s)	223, 547, 1126
MaeI	CTAG	2 cut(s)	293, 1140
MaeII	ACGT	1 cut(s)	167
MaeIII	GTNAC	1 cut(s)	344
MalI	GATC	9 cut(s)	201, 501, 756, 821, 930, 1019, 1029, 1185, 1224
MboI	GATC	9 cut(s)	199, 499, 754, 819, 928, 1017, 1027, 1183, 1222
MflI	RGATCY	3 cut(s)	199, 499, 819
MhlI	GDGCHC	3 cut(s)	774, 845, 1123
MluCI	AATT	3 cut(s)	261, 314, 747
Mly113I	GGCGCC	1 cut(s)	1207
MlyI	GAGTC	1 cut(s)	889
MmeI	TCCRAC	1 cut(s)	114
MroXI	GAANNNNTTC	2 cut(s)	18, 383
MseI	TTAA	2 cut(s)	171, 312
MspI	CCGG	2 cut(s)	121, 1228
MspR9I	CCNGG	3 cut(s)	473, 1228, 1229
Mva1269I	GAATGC	1 cut(s)	216
MvaI	CCWGG	1 cut(s)	473
MwoI	GCNNNNNNNGC	4 cut(s)	254, 1127, 1136, 1145
NarI	GGCGCC	1 cut(s)	1207
NciI	CCSGG	2 cut(s)	1228, 1229
NcoI	CCATGG	1 cut(s)	845
NdeII	GATC	9 cut(s)	199, 499, 754, 819, 928, 1017, 1027, 1183, 1222
NlaIII	CATG	5 cut(s)	415, 610, 849, 1223, 1253
NlaIV	GGNNCC	3 cut(s)	42, 843, 1208
NspI	RCATGY	1 cut(s)	610
NspV	TTCGAA	1 cut(s)	90
PciSI	GCTCTTC	4 cut(s)	87, 110, 1111, 1138
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	1 cut(s)	216
PdmI	GAANNNNTTC	2 cut(s)	18, 383
PfeI	GAWTC	4 cut(s)	14, 373, 596, 731
PleI	GAGTC	1 cut(s)	889
PluTI	GGCGCC	1 cut(s)	1210
PpsI	GAGTC	1 cut(s)	889
PsiI	TTATAA	1 cut(s)	837
Psp1406I	AACGTT	1 cut(s)	167
Psp6I	CCWGG	1 cut(s)	471
PspFI	CCCAGC	1 cut(s)	182
PspGI	CCWGG	1 cut(s)	471
PspN4I	GGNNCC	3 cut(s)	42, 843, 1208
PspOMI	GGGCCC	1 cut(s)	841
PspPI	GGNCC	5 cut(s)	147, 298, 419, 841, 842
PsrI	GAACNNNNNNTAC	2 cut(s)	860, 892
PsuI	RGATCY	3 cut(s)	199, 499, 819
RsaI	GTAC	2 cut(s)	176, 692
RsaNI	GTAC	2 cut(s)	175, 691
SapI	GCTCTTC	4 cut(s)	87, 110, 1111, 1138
SaqAI	TTAA	2 cut(s)	171, 312
Sau3AI	GATC	9 cut(s)	199, 499, 754, 819, 928, 1017, 1027, 1183, 1222
Sau96I	GGNCC	5 cut(s)	147, 298, 419, 841, 842
SchI	GAGTC	1 cut(s)	889
ScrFI	CCNGG	3 cut(s)	473, 1228, 1229
SduI	GDGCHC	3 cut(s)	774, 845, 1123
SfaNI	GCATC	3 cut(s)	223, 547, 1126
SfoI	GGCGCC	1 cut(s)	1208
SfuI	TTCGAA	1 cut(s)	90
SinI	GGWCC	2 cut(s)	298, 419
SmaI	CCCGGG	1 cut(s)	1229
Sse9I	AATT	3 cut(s)	261, 314, 747
SsiI	CCGC	3 cut(s)	44, 342, 390
SspDI	GGCGCC	1 cut(s)	1206
SspI	AATATT	1 cut(s)	305
SspMI	CTAG	2 cut(s)	293, 1140
StyD4I	CCNGG	3 cut(s)	471, 1226, 1227
StyI	CCWWGG	1 cut(s)	845
TaaI	ACNGT	4 cut(s)	407, 569, 704, 1035
TaiI	ACGT	1 cut(s)	170
TaqI	TCGA	4 cut(s)	12, 90, 308, 1005
TasI	AATT	3 cut(s)	261, 314, 747
TatI	WGTACW	2 cut(s)	174, 690
TfiI	GAWTC	4 cut(s)	14, 373, 596, 731
Tru1I	TTAA	2 cut(s)	171, 312
Tru9I	TTAA	2 cut(s)	171, 312
TscAI	CASTG	2 cut(s)	184, 574
TspDTI	ATGAA	2 cut(s)	17, 400
TspGWI	ACGGA	3 cut(s)	53, 813, 888
TspMI	CCCGGG	1 cut(s)	1227
TspRI	CASTG	2 cut(s)	184, 574
VpaK11BI	GGWCC	2 cut(s)	298, 419
XapI	RAATTY	3 cut(s)	261, 314, 747
XceI	RCATGY	1 cut(s)	610
XmaI	CCCGGG	1 cut(s)	1227
XmiI	GTMKAC	1 cut(s)	611
XmnI	GAANNNNTTC	2 cut(s)	18, 383
XspI	CTAG	2 cut(s)	293, 1140

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.