Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002902

Physical Location & Seq

Genomic Coordinates Reverse (-)

32186054 .. 32190200

Gene Structure 4147 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1247000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1275 bp

ATGGCGAAGCAGCAAAAGCAGCTGGTTTTGCTAATATTGTCGCTCTTCTTGCTCTCCTTTTGCTTTCGTTCTTCACTTTCAGAGATCATTTTCGAAGAGCGCTTCGACGACGACGAATGGCGAAGCCGTTGGGTGACATCTGACTGGAAAAGCAGTGAAGGTAAGGCAGGTTCCTTTAAACACACAGCCGGAAAGTGGTCCGGTGACCACGATGATAAAGGAATTCAGACATCTAATGATGCCAAGCATTTTGCCATATCTGCAAAGATACCGGAGTTCAGCAACAGGAACAGGACGTTGGTCCTCCAGTACTCGATCAAGTTTGAGCAGGAAATTGAATGTGGTGGCGGTTACATAAAGCTCATGTCTGGATTTGTTAATCAGAAGAAATTCAGCGGGGACACTCCATACAGTTTAATGTTTGGTCCCGATATTTGCGGCACGGACACAAAGAAGCTCCATGTCATAGTCTCCTACCAAGGCCAGAATTACCCCATCAAGAAGGATTTACAGTGTGAAACTGACAAGTTGACTCATTTCTACACGTTCATTCTGCGACCTGATGCAACTTATAGTGTCCTAATAGACAACCGTGAAAAGGATTCTGGAAGTATGTACACAGATTGGGATATTCTTCCTCCAAGAAAGATAAAAGATGTTACTGCAAAGAAGCCGGCTGACTGGGATCTTAGAGAATACATTGATGATCCCAATTACATCAAACCTGAGGGTTATGATTCAATTCCGAGAGAAATTCCAGATCCAAAAGCAAAACAGCCCGAAGAATGGGATGATGAAGAGAACGGTCTGTGGAAAGCTCCAAAGATACCAAATCCAGCTTATAAAGGACCATGGAGACCCAAGAAAATCAAGAACCCAAATTACAAAGGGAAGTGGAAGATTCCTTGGATTGATAATCCAGAGTTTGAAGATGATCCTGATCTCTATGTGCTTAAGCCAATTAATTATGTTGGTGTCGAAGTTTGGCAGGTAAAGGCCGGTTCAGTTTTCGACAACATTTTAATTTGTGATGATCCTGATTATGCAAAACAAGTGGTCGAGGAAGTCTTCGCCAATAGAGAGGCTGAAAAGGATGCCTTTGAAGAAGCAGAAAAAAGGAGGAAAGCACAGGAGGAAGAGGAGGCTCAAAGAGCAAGAGAAGAGGGAGAAAGGAGGAGAAGAGAGAGGGGTTATGATCGAGGTCGCCGAGACAGACATAGAGACAGATACCGAAGGCACCGTAATTGGGATCACTATGATCATGACGAGCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006457 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009812 GO:0009987 GO:0010204 GO:0012501 GO:0012505 GO:0016020 GO:0023052 GO:0031347 GO:0031349 GO:0031984 GO:0033554 GO:0034050 GO:0042175 GO:0042440 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046283 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071704 GO:0080134 GO:0098542 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko04141 ko04145 ko04612 ko05142 ko05166 map04141 map04145 map04612 map05142 map05166

Pfam Domains

Protein Families

Calreticulin

Protein Analysis

425

Amino Acids

50.01

Weight (kDa)

5.87

Isoelectric Point (pI)

45.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calreticulin	PF00262	31 - 268	9e-57	Calreticulin family
Calreticulin	PF00262	269 - 342	4.2e-19	Calreticulin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002902)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08450 AT1G08450 AT1G08450
fragaria_vesca	FvH4_1g23470 FvH4_1g23470 FvH4_1g23470 FvH4_1g23470 FvH4_1g23470 FvH4_6g00230 FvH4_6g00230 FvH4_6g00230
malus_domestica	MD04G1045300.v1.1 MD04G1247000.v1.1 MD12G1263200.v1.1
prunus_persica	Prupe.1G198500_v2.0.a1 Prupe.6G363200_v2.0.a1
pyrus_communis	pycom04g21770 pycom12g24070
rosa_chinensis	RchiOBHm_Chr2g0118841 RchiOBHm_Chr3g0447601
rosa_laevigata	RLG00000018425 RLG00000025985
rosa_multiflora	Rmu_sc0001814.1_g000009 Rmu_sc0003550.1_g000005
rosa_roxburghii	Rroxscaffold_2G00125130 Rroxscaffold_6G00425810
rosa_rugosa	Rorug02G0219200 Rorug02G0603400
rosa_samantha	Rh2AG276300 Rh2BG288100 Rh2DG283200 Rh2DG302300 Rh3AG003600 Rh3BG003100 Rh3CG003400 Rh3DG003400
rosa_wichuraiana	Rw2G021990 Rw3G000250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	843
Acc36I	ACCTGC	2 cut(s)	158, 979
AccB1I	GGYRCC	1 cut(s)	1236
AciI	CCGC	3 cut(s)	348, 396, 438
AclWI	GGATC	6 cut(s)	693, 701, 755, 929, 1028, 1257
AcsI	RAATTY	3 cut(s)	222, 389, 753
AfaI	GTAC	2 cut(s)	311, 617
AfeI	AGCGCT	1 cut(s)	101
AfiI	CCNNNNNNNGG	4 cut(s)	195, 598, 786, 1246
AflII	CTTAAG	1 cut(s)	953
AflIII	ACRYGT	1 cut(s)	543
AgsI	TTSAA	4 cut(s)	338, 741, 929, 1103
AjuI	GAANNNNNNNTTGG	2 cut(s)	281, 313
AluBI	AGCT	6 cut(s)	22, 361, 457, 818, 839, 1270
AluI	AGCT	6 cut(s)	22, 361, 457, 818, 839, 1270
Alw26I	GTCTC	4 cut(s)	475, 850, 1203, 1215
AlwI	GGATC	6 cut(s)	693, 701, 755, 929, 1028, 1257
Aor51HI	AGCGCT	1 cut(s)	101
AoxI	GGCC	2 cut(s)	481, 996
ApeKI	GCWGC	2 cut(s)	10, 19
ApoI	RAATTY	3 cut(s)	222, 389, 753
AseI	ATTAAT	1 cut(s)	963
Asp700I	GAANNNNTTC	1 cut(s)	389
AspLEI	GCGC	1 cut(s)	102
AspS9I	GGNCC	4 cut(s)	198, 301, 425, 848
AsuHPI	GGTGA	2 cut(s)	145, 215
AsuII	TTCGAA	1 cut(s)	93
AvaII	GGWCC	4 cut(s)	198, 301, 425, 848
AxyI	CCTNAGG	1 cut(s)	726
BaeI	ACNNNNGTAYC	2 cut(s)	260, 293
BanI	GGYRCC	1 cut(s)	1236
BbsI	GAAGAC	1 cut(s)	1060
BbvI	GCAGC	2 cut(s)	22, 31
BccI	CCATC	1 cut(s)	503
BceAI	ACGGC	1 cut(s)	111
BclI	TGATCA	1 cut(s)	1258
BcoDI	GTCTC	4 cut(s)	475, 850, 1203, 1215
BfoI	RGCGCY	1 cut(s)	103
BfrI	CTTAAG	1 cut(s)	953
BfuAI	ACCTGC	2 cut(s)	158, 979
BisI	GCNGC	3 cut(s)	11, 20, 439
BlsI	GCNGC	3 cut(s)	12, 21, 440
BmcAI	AGTACT	1 cut(s)	311
Bme18I	GGWCC	4 cut(s)	198, 301, 425, 848
BmgT120I	GGNCC	4 cut(s)	198, 301, 425, 848
BmiI	GGNNCC	3 cut(s)	172, 427, 1238
BmrI	ACTGGG	1 cut(s)	691
BmsI	GCATC	3 cut(s)	229, 553, 1084
BmuI	ACTGGG	1 cut(s)	691
BoxI	GACNNNNGTC	1 cut(s)	299
BpiI	GAAGAC	1 cut(s)	1060
BpmI	CTGGAG	1 cut(s)	290
Bpu14I	TTCGAA	1 cut(s)	93
BsaBI	GATNNNNATC	1 cut(s)	939
BsaI	GGTCTC	1 cut(s)	850
BsaJI	CCNNGG	3 cut(s)	478, 851, 905
BsaWI	WCCGGW	2 cut(s)	200, 271
Bsc4I	CCNNNNNNNGG	4 cut(s)	195, 598, 786, 1246
Bse118I	RCCGGY	2 cut(s)	673, 998
Bse1I	ACTGG	3 cut(s)	149, 307, 686
Bse21I	CCTNAGG	1 cut(s)	726
Bse8I	GATNNNNATC	1 cut(s)	939
BseDI	CCNNGG	3 cut(s)	478, 851, 905
BseGI	GGATG	2 cut(s)	796, 1099
BseJI	GATNNNNATC	1 cut(s)	939
BseLI	CCNNNNNNNGG	4 cut(s)	195, 598, 786, 1246
BseMII	CTCAG	1 cut(s)	717
BseNI	ACTGG	3 cut(s)	149, 307, 686
BseRI	GAGGAG	2 cut(s)	1154, 1189
BseXI	GCAGC	2 cut(s)	22, 31
BshFI	GGCC	2 cut(s)	483, 998
BshNI	GGYRCC	1 cut(s)	1236
BsiSI	CCGG	5 cut(s)	189, 201, 272, 674, 999
BslFI	GGGAC	2 cut(s)	411, 413
BslI	CCNNNNNNNGG	4 cut(s)	195, 598, 786, 1246
BsmAI	GTCTC	4 cut(s)	475, 850, 1203, 1215
BsmFI	GGGAC	2 cut(s)	411, 413
BsnI	GGCC	2 cut(s)	483, 998
Bso31I	GGTCTC	1 cut(s)	850
Bsp119I	TTCGAA	1 cut(s)	93
Bsp1407I	TGTACA	1 cut(s)	615
Bsp19I	CCATGG	1 cut(s)	851
BspACI	CCGC	3 cut(s)	348, 396, 438
BspANI	GGCC	2 cut(s)	483, 998
BspCNI	CTCAG	1 cut(s)	718
BspHI	TCATGA	1 cut(s)	1261
BspLI	GGNNCC	3 cut(s)	172, 427, 1238
BspMI	ACCTGC	2 cut(s)	158, 979
BspPI	GGATC	6 cut(s)	693, 701, 755, 929, 1028, 1257
BspQI	GCTCTTC	2 cut(s)	50, 90
BspT104I	TTCGAA	1 cut(s)	93
BspT107I	GGYRCC	1 cut(s)	1236
BspTI	CTTAAG	1 cut(s)	953
BspTNI	GGTCTC	1 cut(s)	850
BsrFI	RCCGGY	2 cut(s)	673, 998
BsrGI	TGTACA	1 cut(s)	615
BsrI	ACTGG	3 cut(s)	149, 307, 686
BssAI	RCCGGY	2 cut(s)	673, 998
BssECI	CCNNGG	3 cut(s)	478, 851, 905
BssT1I	CCWWGG	3 cut(s)	478, 851, 905
Bst4CI	ACNGT	5 cut(s)	413, 513, 593, 806, 1241
Bst6I	CTCTTC	6 cut(s)	50, 90, 792, 1131, 1155, 1174
BstAFI	CTTAAG	1 cut(s)	953
BstAUI	TGTACA	1 cut(s)	615
BstBI	TTCGAA	1 cut(s)	93
BstC8I	GCNNGC	1 cut(s)	675
BstDEI	CTNAG	2 cut(s)	689, 726
BstDSI	CCRYGG	1 cut(s)	851
BstEII	GGTNACC	1 cut(s)	203
BstF5I	GGATG	2 cut(s)	796, 1099
BstH2I	RGCGCY	1 cut(s)	103
BstHHI	GCGC	1 cut(s)	102
BstMAI	GTCTC	4 cut(s)	475, 850, 1203, 1215
BstMWI	GCNNNNNNNGC	6 cut(s)	16, 19, 28, 49, 260, 1151
BstPAI	GACNNNNGTC	1 cut(s)	299
BstPI	GGTNACC	1 cut(s)	203
BstV1I	GCAGC	2 cut(s)	22, 31
BstV2I	GAAGAC	1 cut(s)	1060
BstX2I	RGATCY	2 cut(s)	685, 760
BstYI	RGATCY	2 cut(s)	685, 760
Bsu36I	CCTNAGG	1 cut(s)	726
BsuRI	GGCC	2 cut(s)	483, 998
BtgI	CCRYGG	1 cut(s)	851
BtsCI	GGATG	2 cut(s)	796, 1099
BtsI	GCAGTG	1 cut(s)	160
BtsIMutI	CAGTG	2 cut(s)	160, 518
BveI	ACCTGC	2 cut(s)	158, 979
Cac8I	GCNNGC	1 cut(s)	675
CciI	TCATGA	1 cut(s)	1261
CfoI	GCGC	1 cut(s)	102
Cfr10I	RCCGGY	2 cut(s)	673, 998
Cfr13I	GGNCC	4 cut(s)	198, 301, 425, 848
Csp6I	GTAC	2 cut(s)	310, 616
CspCI	CAANNNNNGTGG	2 cut(s)	1035, 1070
CviAII	CATG	4 cut(s)	364, 461, 852, 1262
CviQI	GTAC	2 cut(s)	310, 616
DdeI	CTNAG	2 cut(s)	689, 726
DraI	TTTAAA	1 cut(s)	178
Eam1104I	CTCTTC	6 cut(s)	50, 90, 792, 1131, 1155, 1174
EarI	CTCTTC	6 cut(s)	50, 90, 792, 1131, 1155, 1174
Eco130I	CCWWGG	3 cut(s)	478, 851, 905
Eco31I	GGTCTC	1 cut(s)	850
Eco47I	GGWCC	4 cut(s)	198, 301, 425, 848
Eco47III	AGCGCT	1 cut(s)	101
Eco81I	CCTNAGG	1 cut(s)	726
Eco91I	GGTNACC	1 cut(s)	203
EcoO65I	GGTNACC	1 cut(s)	203
EcoRI	GAATTC	1 cut(s)	222
EcoT14I	CCWWGG	3 cut(s)	478, 851, 905
ErhI	CCWWGG	3 cut(s)	478, 851, 905
FaeI	CATG	4 cut(s)	367, 464, 855, 1265
FalI	AAGNNNNNCTT	2 cut(s)	1082, 1114
FaqI	GGGAC	2 cut(s)	411, 413
FatI	CATG	4 cut(s)	363, 460, 851, 1261
FauI	CCCGC	1 cut(s)	389
FbaI	TGATCA	1 cut(s)	1258
Fnu4HI	GCNGC	3 cut(s)	11, 20, 439
FokI	GGATG	2 cut(s)	803, 1106
Fsp4HI	GCNGC	3 cut(s)	11, 20, 439
GlaI	GCGC	1 cut(s)	101
GluI	GCNGC	3 cut(s)	11, 20, 439
GsuI	CTGGAG	1 cut(s)	290
HaeII	RGCGCY	1 cut(s)	103
HaeIII	GGCC	2 cut(s)	483, 998
HapII	CCGG	5 cut(s)	189, 201, 272, 674, 999
HhaI	GCGC	1 cut(s)	102
Hin1II	CATG	4 cut(s)	367, 464, 855, 1265
Hin6I	GCGC	1 cut(s)	100
HinP1I	GCGC	1 cut(s)	100
HincII	GTYRAC	1 cut(s)	531
HindII	GTYRAC	1 cut(s)	531
HinfI	GANTC	4 cut(s)	532, 602, 737, 901
HpaII	CCGG	5 cut(s)	189, 201, 272, 674, 999
HphI	GGTGA	2 cut(s)	145, 215
Hpy166II	GTNNAC	2 cut(s)	531, 618
Hpy188I	TCNGA	5 cut(s)	82, 142, 228, 384, 747
Hpy8I	GTNNAC	2 cut(s)	531, 618
Hpy99I	CGWCG	3 cut(s)	110, 113, 116
HpyAV	CCTTC	3 cut(s)	152, 496, 1227
HpyCH4III	ACNGT	5 cut(s)	413, 513, 593, 806, 1241
HpyCH4IV	ACGT	2 cut(s)	296, 545
HpyCH4V	TGCA	4 cut(s)	263, 566, 665, 1046
HpyF10VI	GCNNNNNNNGC	6 cut(s)	16, 19, 28, 49, 260, 1151
HpyF3I	CTNAG	2 cut(s)	689, 726
HpySE526I	ACGT	2 cut(s)	296, 545
Hsp92II	CATG	4 cut(s)	367, 464, 855, 1265
HspAI	GCGC	1 cut(s)	100
KroI	GCCGGC	1 cut(s)	673
KroNI	GCCGGC	1 cut(s)	675
Ksp22I	TGATCA	1 cut(s)	1258
LguI	GCTCTTC	2 cut(s)	50, 90
LmnI	GCTCC	2 cut(s)	462, 823
Lsp1109I	GCAGC	2 cut(s)	22, 31
LweI	GCATC	3 cut(s)	229, 553, 1084
MaeII	ACGT	2 cut(s)	296, 545
MaeIII	GTNAC	4 cut(s)	133, 203, 350, 658
MflI	RGATCY	2 cut(s)	685, 760
MlyI	GAGTC	1 cut(s)	526
MroNI	GCCGGC	1 cut(s)	673
MroXI	GAANNNNTTC	1 cut(s)	389
MseI	TTAA	6 cut(s)	177, 378, 416, 954, 963, 1022
MspA1I	CMGCKG	2 cut(s)	22, 396
MspCI	CTTAAG	1 cut(s)	953
MspI	CCGG	5 cut(s)	189, 201, 272, 674, 999
MwoI	GCNNNNNNNGC	6 cut(s)	16, 19, 28, 49, 260, 1151
NaeI	GCCGGC	1 cut(s)	675
NcoI	CCATGG	1 cut(s)	851
NgoMIV	GCCGGC	1 cut(s)	673
NlaIII	CATG	4 cut(s)	367, 464, 855, 1265
NlaIV	GGNNCC	3 cut(s)	172, 427, 1238
NmeAIII	GCCGAG	1 cut(s)	1232
NmuCI	GTSAC	2 cut(s)	133, 203
NspV	TTCGAA	1 cut(s)	93
PagI	TCATGA	1 cut(s)	1261
PciSI	GCTCTTC	2 cut(s)	50, 90
PcsI	WCGNNNNNNNCGW	2 cut(s)	111, 1204
PdiI	GCCGGC	1 cut(s)	675
PdmI	GAANNNNTTC	1 cut(s)	389
PfeI	GAWTC	3 cut(s)	602, 737, 901
PkrI	GCNGC	3 cut(s)	12, 21, 440
PleI	GAGTC	1 cut(s)	526
PpsI	GAGTC	1 cut(s)	526
PshAI	GACNNNNGTC	1 cut(s)	299
PshBI	ATTAAT	1 cut(s)	963
PsiI	TTATAA	1 cut(s)	843
PspEI	GGTNACC	1 cut(s)	203
PspN4I	GGNNCC	3 cut(s)	172, 427, 1238
PspPI	GGNCC	4 cut(s)	198, 301, 425, 848
PsrI	GAACNNNNNNTAC	4 cut(s)	154, 186, 866, 898
PsuI	RGATCY	2 cut(s)	685, 760
PvuII	CAGCTG	1 cut(s)	22
RsaI	GTAC	2 cut(s)	311, 617
RsaNI	GTAC	2 cut(s)	310, 616
SapI	GCTCTTC	2 cut(s)	50, 90
SaqAI	TTAA	6 cut(s)	177, 378, 416, 954, 963, 1022
SatI	GCNGC	3 cut(s)	11, 20, 439
Sau96I	GGNCC	4 cut(s)	198, 301, 425, 848
ScaI	AGTACT	1 cut(s)	311
SchI	GAGTC	1 cut(s)	526
SfaNI	GCATC	3 cut(s)	229, 553, 1084
SfuI	TTCGAA	1 cut(s)	93
SinI	GGWCC	4 cut(s)	198, 301, 425, 848
SmlI	CTYRAG	1 cut(s)	953
SmoI	CTYRAG	1 cut(s)	953
SsiI	CCGC	3 cut(s)	348, 396, 438
SspI	AATATT	1 cut(s)	36
StyI	CCWWGG	3 cut(s)	478, 851, 905
TaaI	ACNGT	5 cut(s)	413, 513, 593, 806, 1241
TaiI	ACGT	2 cut(s)	299, 548
TaqI	TCGA	7 cut(s)	93, 105, 314, 978, 1011, 1059, 1198
TatI	WGTACW	2 cut(s)	309, 615
TauI	GCSGC	1 cut(s)	441
TfiI	GAWTC	3 cut(s)	602, 737, 901
Tru1I	TTAA	6 cut(s)	177, 378, 416, 954, 963, 1022
Tru9I	TTAA	6 cut(s)	177, 378, 416, 954, 963, 1022
TscAI	CASTG	2 cut(s)	160, 518
TseFI	GTSAC	2 cut(s)	133, 203
TseI	GCWGC	2 cut(s)	10, 19
Tsp45I	GTSAC	2 cut(s)	133, 203
TspDTI	ATGAA	2 cut(s)	538, 810
TspGWI	ACGGA	1 cut(s)	458
TspRI	CASTG	2 cut(s)	160, 518
Vha464I	CTTAAG	1 cut(s)	953
VpaK11BI	GGWCC	4 cut(s)	198, 301, 425, 848
VspI	ATTAAT	1 cut(s)	963
XapI	RAATTY	3 cut(s)	222, 389, 753
XmnI	GAANNNNTTC	1 cut(s)	389
ZrmI	AGTACT	1 cut(s)	311

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.