Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001809

Physical Location & Seq

Genomic Coordinates Forward (+)

5916101 .. 5919099

Gene Structure 2999 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1050100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1080 bp

ATGGCAACCGCGAACCCTTTCGATCTCTTGGGCGATGACGACAACGAGGACCCAAGTCAACTTCTCGCTGCTCAGCAGCAGAAGCTGCCGGCGGCGCAGCCCAAGAAGCCCCAGCAGGCCCAGCCTGCTCAGCCTGCTAAGCTTCCCTCCAAGCCACTCCCCCCTGCACAATCTGTGAGGGAGGGAAAGTATGAAGGTGGCCGTGGAGGTGGCCGTGGTGGACGTGGGTTCAGTCGTGGCCGTGGTGGAGGTGGTGGTGGATTTAATAGAGATCCAATCAGCAATGAGAACACCAATGGTTTTTCTGGTGGCTACAGACCATCTGAAGAGGGAGATGGGAAAGCTTCTGAAAGACGCGGGGGCTATGGTGCGCCTCGTGGTTCCTTCCGTGGTGGGCGTCGTGGTGGTTACAACAATGGTGAGGATGGTGAGGGAGAACGCCCTCGGAGGGTATTTGATCGTCGCAGTGGTACAGGACGTGGGAGTGATTTCAAACGTGAAGGGTCTGGTCGGGGGAATTGGGGAACTCCCTCTGATGACATTGCTCCGGAGACTGAGGAGCCTGGAACTGAAACTGAGAAGAATGTTGGTGCTGAGAAGCAGTCGGGAGAGAATGAGGCTGCTGATGCCAATAAGGAGGATACTGTTAATGAGACGGAAGAGAAGGAGCCTGAGGAGAAGGAAATGACTCTGGAAGAGTATCAGAAGGTGCTTGAAGAGAAGAGGAAGGCTCTGCTTGCATTGAAGACTGAGGAAAGAAAGGTTGATGTGGACAAAGACTTGAAGGCCATGCAGCTGCTTTCAAACAAGAAGGAAAATGATGAAATATTTATCAAGCTGGGTTCGGAGAAGGATAAGCGCAAAGAAGCTGCTGAGAAAGAAGAGAGAGCCAAGAAGTCTGTTAGCATTAATGAGTTTCTGAAGCCCGCTGAAGGCGAGAGGTACCATGGTGGTCGTGGTCGAGGCCGTGGCCGTGGGCCAAGAGGTGGATACAGTGGAGGACCTGGAGGTTACTCAAGCAATGCGGCAGCACCCTCCATTGGGGATCCCGGCCAGTTCCCATCCCTCGGCGGGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HABP4_PAI-RBP1 Stm1_N

Protein Analysis

360

Amino Acids

38.52

Weight (kDa)

6.76

Isoelectric Point (pI)

46.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stm1_N	PF09598	1 - 72	2.1e-24	Stm1
HABP4_PAI-RBP1	PF04774	148 - 255	1.6e-29	Hyaluronan / mRNA binding family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16830 AT4G16830 AT4G16830
fragaria_vesca	FvH4_5g20020 FvH4_5g20020 FvH4_5g20020 FvH4_7g10280
malus_domestica	MD02G1213500.v1.1 MD04G1050100.v1.1 MD06G1049800.v1.1 MD07G1109500.v1.1
prunus_persica	Prupe.2G132500_v2.0.a1 Prupe.5G060200_v2.0.a1 Prupe.5G060200_v2.0.a1 Prupe.5G060200_v2.0.a1
pyrus_communis	pycom04g04410 pycom06g04220 pycom07g09400 pycom12646g00030
rosa_chinensis	RchiOBHm_Chr1g0348031 RchiOBHm_Chr7g0204831 RchiOBHm_Chr7g0204851
rosa_laevigata	RLG00000003428 RLG00000028697
rosa_multiflora	Rmu_sc0002180.1_g000016 Rmu_sc0002180.1_g000023 Rmu_ssc0000263.1_g000007
rosa_roxburghii	Rroxscaffold_3G00252730 Rroxscaffold_4G00307320
rosa_rugosa	Rorug01G0190700 Rorug01G0190800 Rorug07G0086000 Rorug07G0086000 Rorug07G0086100
rosa_samantha	Rh1AG209300 Rh1BG174300 Rh1CG193700 Rh1DG204100 Rh7AG216500 Rh7AG216700 Rh7BG213600 Rh7CG230000 Rh7DG224100 Rh7DG224300
rosa_wichuraiana	Rw1G017440 Rw7G018760 Rw7G018780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	942
AccB1I	GGYRCC	1 cut(s)	942
AccB7I	CCANNNNNTGG	1 cut(s)	986
AccII	CGCG	2 cut(s)	11, 357
AccIII	TCCGGA	1 cut(s)	547
AciI	CCGC	6 cut(s)	9, 92, 357, 927, 1025, 1071
AclWI	GGATC	3 cut(s)	266, 1040, 1053
AcoI	YGGCCR	5 cut(s)	199, 211, 238, 970, 1051
AcuI	CTGAAG	3 cut(s)	345, 941, 951
AcyI	GRCGYC	1 cut(s)	397
AfaI	GTAC	2 cut(s)	472, 944
AfiI	CCNNNNNNNGG	7 cut(s)	448, 932, 986, 1040, 1041, 1067, 1071
AgsI	TTSAA	5 cut(s)	493, 716, 745, 784, 804
AjiI	CACGTC	2 cut(s)	224, 479
AjnI	CCWGG	2 cut(s)	562, 1003
AluBI	AGCT	6 cut(s)	85, 142, 344, 796, 838, 869
AluI	AGCT	6 cut(s)	85, 142, 344, 796, 838, 869
Alw26I	GTCTC	2 cut(s)	545, 647
AlwI	GGATC	3 cut(s)	266, 1040, 1053
AlwNI	CAGNNNCTG	1 cut(s)	85
Aor13HI	TCCGGA	1 cut(s)	547
AoxI	GGCC	9 cut(s)	117, 199, 211, 238, 786, 964, 970, 977, 1051
ApeKI	GCWGC	9 cut(s)	68, 76, 85, 97, 620, 793, 796, 869, 1028
AseI	ATTAAT	1 cut(s)	909
Asp700I	GAANNNNTTC	1 cut(s)	17
Asp718I	GGTACC	1 cut(s)	942
AspLEI	GCGC	3 cut(s)	97, 373, 861
AspS9I	GGNCC	4 cut(s)	49, 118, 977, 1001
AsuC2I	CCSGG	1 cut(s)	1050
AsuHPI	GGTGA	2 cut(s)	431, 440
AvaII	GGWCC	2 cut(s)	49, 1001
AxyI	CCTNAGG	1 cut(s)	672
BaeI	ACNNNNGTAYC	2 cut(s)	934, 967
BamHI	GGATCC	1 cut(s)	1045
BanI	GGYRCC	1 cut(s)	942
BauI	CACGAG	1 cut(s)	375
BbsI	GAAGAC	1 cut(s)	752
BbvI	GCAGC	9 cut(s)	55, 72, 88, 109, 607, 783, 805, 856, 1040
BccI	CCATC	4 cut(s)	328, 329, 419, 1069
BceAI	ACGGC	5 cut(s)	186, 198, 225, 951, 957
BciT130I	CCWGG	2 cut(s)	564, 1005
BciVI	GTATCC	2 cut(s)	634, 983
BcnI	CCSGG	1 cut(s)	1050
BcoDI	GTCTC	2 cut(s)	545, 647
BfmI	CTRYAG	1 cut(s)	313
BfuI	GTATCC	2 cut(s)	634, 983
BlpI	GCTNAGC	3 cut(s)	72, 129, 138
Bme1390I	CCNGG	3 cut(s)	564, 1005, 1050
Bme18I	GGWCC	2 cut(s)	49, 1001
BmgBI	CACGTC	2 cut(s)	224, 479
BmgT120I	GGNCC	4 cut(s)	49, 118, 977, 1001
BmiI	GGNNCC	6 cut(s)	51, 382, 561, 669, 944, 1047
BmrFI	CCNGG	3 cut(s)	564, 1005, 1050
BmsI	GCATC	1 cut(s)	616
BoxI	GACNNNNGTC	1 cut(s)	54
BpiI	GAAGAC	1 cut(s)	752
BplI	GAGNNNNNCTC	2 cut(s)	715, 747
BpmI	CTGGAG	1 cut(s)	1026
Bpu1102I	GCTNAGC	3 cut(s)	72, 129, 138
BpuEI	CTTGAG	1 cut(s)	1000
BpuMI	CCSGG	1 cut(s)	1050
BsaHI	GRCGYC	1 cut(s)	397
BsaJI	CCNNGG	9 cut(s)	202, 214, 241, 388, 443, 946, 967, 973, 1066
BsaWI	WCCGGW	1 cut(s)	547
BsaXI	ACNNNNNCTCC	2 cut(s)	173, 203
Bsc4I	CCNNNNNNNGG	7 cut(s)	448, 932, 986, 1040, 1041, 1067, 1071
Bse118I	RCCGGY	1 cut(s)	88
Bse1I	ACTGG	1 cut(s)	1054
Bse21I	CCTNAGG	1 cut(s)	672
Bse3DI	GCAATG	3 cut(s)	289, 540, 1027
BseAI	TCCGGA	1 cut(s)	547
BseBI	CCWGG	2 cut(s)	564, 1005
BseDI	CCNNGG	9 cut(s)	202, 214, 241, 388, 443, 946, 967, 973, 1066
BseGI	GGATG	2 cut(s)	430, 1061
BseLI	CCNNNNNNNGG	7 cut(s)	448, 932, 986, 1040, 1041, 1067, 1071
BseMI	GCAATG	3 cut(s)	289, 540, 1027
BseMII	CTCAG	8 cut(s)	86, 143, 546, 567, 585, 663, 741, 864
BseNI	ACTGG	1 cut(s)	1054
BseRI	GAGGAG	2 cut(s)	572, 689
BseXI	GCAGC	9 cut(s)	55, 72, 88, 109, 607, 783, 805, 856, 1040
BseYI	CCCAGC	3 cut(s)	111, 120, 838
BsgI	GTGCAG	1 cut(s)	150
Bsh1236I	CGCG	2 cut(s)	11, 357
BshFI	GGCC	9 cut(s)	119, 201, 213, 240, 788, 966, 972, 979, 1053
BshNI	GGYRCC	1 cut(s)	942
BsiSI	CCGG	3 cut(s)	89, 548, 1050
BslI	CCNNNNNNNGG	7 cut(s)	448, 932, 986, 1040, 1041, 1067, 1071
BsmAI	GTCTC	2 cut(s)	545, 647
BsmBI	CGTCTC	1 cut(s)	647
BsnI	GGCC	9 cut(s)	119, 201, 213, 240, 788, 966, 972, 979, 1053
Bsp13I	TCCGGA	1 cut(s)	547
Bsp143I	GATC	4 cut(s)	22, 271, 457, 1045
Bsp1720I	GCTNAGC	3 cut(s)	72, 129, 138
Bsp19I	CCATGG	1 cut(s)	946
BspACI	CCGC	6 cut(s)	9, 92, 357, 927, 1025, 1071
BspANI	GGCC	9 cut(s)	119, 201, 213, 240, 788, 966, 972, 979, 1053
BspCNI	CTCAG	8 cut(s)	85, 142, 547, 568, 586, 664, 742, 865
BspEI	TCCGGA	1 cut(s)	547
BspFNI	CGCG	2 cut(s)	11, 357
BspLI	GGNNCC	6 cut(s)	51, 382, 561, 669, 944, 1047
BspPI	GGATC	3 cut(s)	266, 1040, 1053
BspT107I	GGYRCC	1 cut(s)	942
BsrDI	GCAATG	3 cut(s)	289, 540, 1027
BsrFI	RCCGGY	1 cut(s)	88
BsrI	ACTGG	1 cut(s)	1054
BssAI	RCCGGY	1 cut(s)	88
BssECI	CCNNGG	9 cut(s)	202, 214, 241, 388, 443, 946, 967, 973, 1066
BssMI	GATC	4 cut(s)	22, 271, 457, 1045
BssNI	GRCGYC	1 cut(s)	397
BssSI	CACGAG	1 cut(s)	375
BssT1I	CCWWGG	1 cut(s)	946
Bst2BI	CACGAG	1 cut(s)	375
Bst2UI	CCWGG	2 cut(s)	564, 1005
Bst4CI	ACNGT	2 cut(s)	646, 995
Bst6I	CTCTTC	6 cut(s)	321, 654, 690, 711, 716, 876
BstACI	GRCGYC	1 cut(s)	397
BstAPI	GCANNNNNTGC	1 cut(s)	85
BstC8I	GCNNGC	6 cut(s)	90, 117, 126, 135, 738, 927
BstDEI	CTNAG	9 cut(s)	72, 129, 138, 555, 576, 594, 672, 750, 873
BstDSI	CCRYGG	7 cut(s)	202, 214, 241, 388, 946, 967, 973
BstF5I	GGATG	2 cut(s)	430, 1061
BstFNI	CGCG	2 cut(s)	11, 357
BstHHI	GCGC	3 cut(s)	97, 373, 861
BstKTI	GATC	4 cut(s)	25, 274, 460, 1048
BstMAI	GTCTC	2 cut(s)	545, 647
BstMBI	GATC	4 cut(s)	22, 271, 457, 1045
BstNI	CCWGG	2 cut(s)	564, 1005
BstPAI	GACNNNNGTC	1 cut(s)	54
BstSCI	CCNGG	3 cut(s)	562, 1003, 1048
BstSFI	CTRYAG	1 cut(s)	313
BstUI	CGCG	2 cut(s)	11, 357
BstV1I	GCAGC	9 cut(s)	55, 72, 88, 109, 607, 783, 805, 856, 1040
BstV2I	GAAGAC	1 cut(s)	752
BstX2I	RGATCY	2 cut(s)	271, 1045
BstYI	RGATCY	2 cut(s)	271, 1045
Bsu36I	CCTNAGG	1 cut(s)	672
BsuI	GTATCC	2 cut(s)	634, 983
BsuRI	GGCC	9 cut(s)	119, 201, 213, 240, 788, 966, 972, 979, 1053
BtgI	CCRYGG	7 cut(s)	202, 214, 241, 388, 946, 967, 973
BtgZI	GCGATG	1 cut(s)	48
BtrI	CACGTC	2 cut(s)	224, 479
BtsCI	GGATG	2 cut(s)	430, 1061
BtsI	GCAGTG	1 cut(s)	472
BtsIMutI	CAGTG	2 cut(s)	472, 1000
Cac8I	GCNNGC	6 cut(s)	90, 117, 126, 135, 738, 927
CaiI	CAGNNNCTG	1 cut(s)	85
CfoI	GCGC	3 cut(s)	97, 373, 861
Cfr10I	RCCGGY	1 cut(s)	88
Cfr13I	GGNCC	4 cut(s)	49, 118, 977, 1001
CseI	GACGC	2 cut(s)	363, 386
Csp6I	GTAC	2 cut(s)	471, 943
CviAII	CATG	2 cut(s)	790, 947
CviQI	GTAC	2 cut(s)	471, 943
DdeI	CTNAG	9 cut(s)	72, 129, 138, 555, 576, 594, 672, 750, 873
DpnI	GATC	4 cut(s)	24, 273, 459, 1047
DpnII	GATC	4 cut(s)	22, 271, 457, 1045
EaeI	YGGCCR	5 cut(s)	199, 211, 238, 970, 1051
Eam1104I	CTCTTC	6 cut(s)	321, 654, 690, 711, 716, 876
EarI	CTCTTC	6 cut(s)	321, 654, 690, 711, 716, 876
Eco130I	CCWWGG	1 cut(s)	946
Eco47I	GGWCC	2 cut(s)	49, 1001
Eco57I	CTGAAG	3 cut(s)	345, 941, 951
Eco81I	CCTNAGG	1 cut(s)	672
EcoO109I	RGGNCCY	2 cut(s)	49, 1001
EcoRII	CCWGG	2 cut(s)	562, 1003
EcoT14I	CCWWGG	1 cut(s)	946
ErhI	CCWWGG	1 cut(s)	946
Esp3I	CGTCTC	1 cut(s)	647
FaeI	CATG	2 cut(s)	793, 950
FaiI	YATR	4 cut(s)	192, 366, 791, 948
FatI	CATG	2 cut(s)	789, 946
FauI	CCCGC	3 cut(s)	350, 934, 1064
FokI	GGATG	2 cut(s)	437, 1048
GlaI	GCGC	3 cut(s)	96, 372, 860
GsaI	CCCAGC	3 cut(s)	115, 124, 842
GsuI	CTGGAG	1 cut(s)	1026
HaeIII	GGCC	9 cut(s)	119, 201, 213, 240, 788, 966, 972, 979, 1053
HapII	CCGG	3 cut(s)	89, 548, 1050
HgaI	GACGC	2 cut(s)	363, 386
HhaI	GCGC	3 cut(s)	97, 373, 861
Hin1I	GRCGYC	1 cut(s)	397
Hin1II	CATG	2 cut(s)	793, 950
Hin6I	GCGC	3 cut(s)	95, 371, 859
HinP1I	GCGC	3 cut(s)	95, 371, 859
HincII	GTYRAC	1 cut(s)	59
HindII	GTYRAC	1 cut(s)	59
HindIII	AAGCTT	2 cut(s)	140, 342
HinfI	GANTC	1 cut(s)	688
HpaII	CCGG	3 cut(s)	89, 548, 1050
HphI	GGTGA	2 cut(s)	431, 440
Hpy166II	GTNNAC	3 cut(s)	59, 221, 772
Hpy188I	TCNGA	7 cut(s)	325, 349, 447, 535, 705, 847, 921
Hpy188III	TCNNGA	3 cut(s)	548, 606, 692
Hpy8I	GTNNAC	3 cut(s)	59, 221, 772
Hpy99I	CGWCG	2 cut(s)	402, 465
HpyCH4III	ACNGT	2 cut(s)	646, 995
HpyCH4IV	ACGT	3 cut(s)	223, 478, 496
HpyCH4V	TGCA	3 cut(s)	167, 740, 793
HpyF3I	CTNAG	9 cut(s)	72, 129, 138, 555, 576, 594, 672, 750, 873
HpySE526I	ACGT	3 cut(s)	223, 478, 496
Hsp92I	GRCGYC	1 cut(s)	397
Hsp92II	CATG	2 cut(s)	793, 950
HspAI	GCGC	3 cut(s)	95, 371, 859
Kpn2I	TCCGGA	1 cut(s)	547
KpnI	GGTACC	1 cut(s)	946
KroI	GCCGGC	1 cut(s)	88
KroNI	GCCGGC	1 cut(s)	90
Kzo9I	GATC	4 cut(s)	22, 271, 457, 1045
LmnI	GCTCC	3 cut(s)	550, 559, 667
Lsp1109I	GCAGC	9 cut(s)	55, 72, 88, 109, 607, 783, 805, 856, 1040
LweI	GCATC	1 cut(s)	616
MaeII	ACGT	3 cut(s)	223, 478, 496
MaeIII	GTNAC	2 cut(s)	407, 1010
MalI	GATC	4 cut(s)	24, 273, 459, 1047
MboI	GATC	4 cut(s)	22, 271, 457, 1045
MboII	GAAGA	8 cut(s)	338, 592, 671, 707, 728, 733, 757, 893
MflI	RGATCY	2 cut(s)	271, 1045
MluCI	AATT	1 cut(s)	517
MlyI	GAGTC	1 cut(s)	682
MroI	TCCGGA	1 cut(s)	547
MroNI	GCCGGC	1 cut(s)	88
MroXI	GAANNNNTTC	1 cut(s)	17
MseI	TTAA	3 cut(s)	264, 648, 909
MspA1I	CMGCKG	2 cut(s)	796, 929
MspI	CCGG	3 cut(s)	89, 548, 1050
MspR9I	CCNGG	3 cut(s)	564, 1005, 1050
MvaI	CCWGG	2 cut(s)	564, 1005
MvnI	CGCG	2 cut(s)	11, 357
NaeI	GCCGGC	1 cut(s)	90
NciI	CCSGG	1 cut(s)	1050
NcoI	CCATGG	1 cut(s)	946
NdeII	GATC	4 cut(s)	22, 271, 457, 1045
NgoMIV	GCCGGC	1 cut(s)	88
NlaIII	CATG	2 cut(s)	793, 950
NlaIV	GGNNCC	6 cut(s)	51, 382, 561, 669, 944, 1047
NmeAIII	GCCGAG	1 cut(s)	1047
PdiI	GCCGGC	1 cut(s)	90
PdmI	GAANNNNTTC	1 cut(s)	17
PflMI	CCANNNNNTGG	1 cut(s)	986
PleI	GAGTC	1 cut(s)	682
PpsI	GAGTC	1 cut(s)	682
PpuMI	RGGWCCY	2 cut(s)	49, 1001
PshAI	GACNNNNGTC	1 cut(s)	54
PshBI	ATTAAT	1 cut(s)	909
Psp5II	RGGWCCY	2 cut(s)	49, 1001
Psp6I	CCWGG	2 cut(s)	562, 1003
PspFI	CCCAGC	3 cut(s)	111, 120, 838
PspGI	CCWGG	2 cut(s)	562, 1003
PspN4I	GGNNCC	6 cut(s)	51, 382, 561, 669, 944, 1047
PspPI	GGNCC	4 cut(s)	49, 118, 977, 1001
PspPPI	RGGWCCY	2 cut(s)	49, 1001
PstNI	CAGNNNCTG	1 cut(s)	85
PsuI	RGATCY	2 cut(s)	271, 1045
PvuII	CAGCTG	1 cut(s)	796
RsaI	GTAC	2 cut(s)	472, 944
RsaNI	GTAC	2 cut(s)	471, 943
SaqAI	TTAA	3 cut(s)	264, 648, 909
Sau3AI	GATC	4 cut(s)	22, 271, 457, 1045
Sau96I	GGNCC	4 cut(s)	49, 118, 977, 1001
SchI	GAGTC	1 cut(s)	682
ScrFI	CCNGG	3 cut(s)	564, 1005, 1050
SfaNI	GCATC	1 cut(s)	616
SfcI	CTRYAG	1 cut(s)	313
SinI	GGWCC	2 cut(s)	49, 1001
SmlI	CTYRAG	1 cut(s)	1015
SmoI	CTYRAG	1 cut(s)	1015
Sse9I	AATT	1 cut(s)	517
SsiI	CCGC	6 cut(s)	9, 92, 357, 927, 1025, 1071
SspI	AATATT	1 cut(s)	828
StyD4I	CCNGG	3 cut(s)	562, 1003, 1048
StyI	CCWWGG	1 cut(s)	946
TaaI	ACNGT	2 cut(s)	646, 995
TaiI	ACGT	3 cut(s)	226, 481, 499
TaqI	TCGA	2 cut(s)	21, 961
TasI	AATT	1 cut(s)	517
TauI	GCSGC	2 cut(s)	95, 1028
Tru1I	TTAA	3 cut(s)	264, 648, 909
Tru9I	TTAA	3 cut(s)	264, 648, 909
TscAI	CASTG	2 cut(s)	472, 1000
TseI	GCWGC	9 cut(s)	68, 76, 85, 97, 620, 793, 796, 869, 1028
TspDTI	ATGAA	2 cut(s)	207, 837
TspGWI	ACGGA	2 cut(s)	377, 671
TspRI	CASTG	2 cut(s)	472, 1000
Van91I	CCANNNNNTGG	1 cut(s)	986
VpaK11BI	GGWCC	2 cut(s)	49, 1001
VspI	ATTAAT	1 cut(s)	909
XcmI	CCANNNNNNNNNTGG	1 cut(s)	953
XmnI	GAANNNNTTC	1 cut(s)	17

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.