Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001854

Physical Location & Seq

Genomic Coordinates Reverse (-)

19216567 .. 19220090

Gene Structure 3524 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1104700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 525 bp

ATGGCGGAAGCTCCCGTGAGTCCAGGCGGCGGCAGCCACGAGAGCGGCGGCGACGGGAGCGGAAGCCCGCGCTCCAACGTTCGGGAGCAGGACCGGTATCTTCCGATCGCCAACATCAGCCGTATCATGAAGAAGGCGCTTCCTCCGAACGGCAAAATCGCCAAGGATGCCAAGGAAACCGTCCAGGAATGCGTTTCTGAGTTCATCAGCTTCATCACCTCCGAGGCGAGTGACAAGTGCCAGAGAGAGAAGAGGAAGACGATCAACGGTGATGATTTGCTCTGGGCAATGGCAACTTTAGGCTTTGAGGATTATATCGACCCCCTCAAGGTTTACCTCACCAAATACAGAGAGGTGGAGGGTGATACCAAGGGCTCGGGGAAGGGCGGAGATTCATCTTCCAAGAAAGATGCTCAGCCAAGTCCAAATCCTCAGATTGCTCGCCAAGGTTCTTTCTCCCAAGGAGGGAATTACTCAAATTCTCAAAACCAATACAAGATAGCTCCTATGCAAGGGTCAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006082 GO:0006355 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016143 GO:0019219 GO:0019222 GO:0019748 GO:0019757 GO:0019760 GO:0031323 GO:0031326 GO:0043436 GO:0043565 GO:0044237 GO:0044281 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0140110 GO:1901135 GO:1901363 GO:1901564 GO:1901657 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04612 ko05152 ko05166 map04612 map05152 map05166

Pfam Domains

Protein Families

CBFD_NFYB_HMF

Protein Analysis

175

Amino Acids

18.78

Weight (kDa)

6.62

Isoelectric Point (pI)

46.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBFD_NFYB_HMF	PF00808	33 - 97	6.3e-28	Histone-like transcription factor (CBF/NF-Y) and archaeal histone

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001854)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37060 AT2G37060 AT2G37060 AT3G53340 AT3G53340 AT3G53340 AT3G53340 AT3G53340
fragaria_vesca	FvH4_6g15260 FvH4_6g15260 FvH4_6g15260 FvH4_6g15260 FvH4_6g15260
malus_domestica	MD04G1104700.v1.1 MD12G1124800.v1.1
prunus_persica	Prupe.6G241800_v2.0.a1 Prupe.6G241800_v2.0.a1 Prupe.6G241800_v2.0.a1 Prupe.6G241800_v2.0.a1 Prupe.6G241800_v2.0.a1
pyrus_communis	pycom04g09970 pycom12g12320
rosa_chinensis	RchiOBHm_Chr2g0145071 RchiOBHm_Chr3g0468661 RchiOBHm_Chr5g0066261
rosa_laevigata	RLG00000024032 RLG00000024417
rosa_multiflora	Rmu_co8446349.1_g000001 Rmu_sc0009516.1_g000001 Rmu_sc0011538.1_g000009
rosa_roxburghii	Rroxscaffold_1G00010930 Rroxscaffold_6G00412670
rosa_rugosa	Rorug03G0096100 Rorug03G0096200 Rorug03G0096200 Rorug03G0096200
rosa_samantha	Rh1AG429500 Rh1BG152900 Rh2AG054200 Rh2DG539500 Rh3AG144900 Rh3BG167300 Rh3BG326700 Rh3DG166200
rosa_wichuraiana	Rw3G013660 Rw6G026160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	45, 60
AccII	CGCG	1 cut(s)	70
AciI	CCGC	8 cut(s)	5, 27, 30, 45, 48, 60, 68, 387
AclI	AACGTT	1 cut(s)	78
AcsI	RAATTY	1 cut(s)	478
AfiI	CCNNNNNNNGG	6 cut(s)	29, 81, 149, 328, 465, 512
AgeI	ACCGGT	1 cut(s)	93
AjnI	CCWGG	2 cut(s)	22, 183
AluBI	AGCT	3 cut(s)	11, 210, 503
AluI	AGCT	3 cut(s)	11, 210, 503
Ama87I	CYCGRG	1 cut(s)	376
ApeKI	GCWGC	1 cut(s)	33
ApoI	RAATTY	1 cut(s)	478
AsiGI	ACCGGT	1 cut(s)	93
AspLEI	GCGC	2 cut(s)	72, 139
AspS9I	GGNCC	1 cut(s)	91
AsuHPI	GGTGA	4 cut(s)	208, 281, 331, 374
AvaI	CYCGRG	1 cut(s)	376
AvaII	GGWCC	1 cut(s)	91
BanII	GRGCYC	1 cut(s)	377
BauI	CACGAG	1 cut(s)	38
BbsI	GAAGAC	1 cut(s)	263
BbvI	GCAGC	1 cut(s)	45
BceAI	ACGGC	2 cut(s)	105, 166
BciT130I	CCWGG	2 cut(s)	24, 185
BfoI	RGCGCY	1 cut(s)	140
BisI	GCNGC	5 cut(s)	28, 31, 34, 46, 49
BlpI	GCTNAGC	1 cut(s)	414
BlsI	GCNGC	5 cut(s)	29, 32, 35, 47, 50
Bme1390I	CCNGG	2 cut(s)	24, 185
Bme18I	GGWCC	1 cut(s)	91
BmeT110I	CYCGRG	1 cut(s)	376
BmgT120I	GGNCC	1 cut(s)	91
BmrFI	CCNGG	2 cut(s)	24, 185
BmsI	GCATC	2 cut(s)	157, 400
BpiI	GAAGAC	1 cut(s)	263
Bpu1102I	GCTNAGC	1 cut(s)	414
BpuEI	CTTGAG	1 cut(s)	311
BsaJI	CCNNGG	6 cut(s)	162, 171, 222, 369, 445, 460
BsaWI	WCCGGW	1 cut(s)	93
Bsc4I	CCNNNNNNNGG	6 cut(s)	29, 81, 149, 328, 465, 512
Bse118I	RCCGGY	1 cut(s)	93
Bse3DI	GCAATG	1 cut(s)	294
BseBI	CCWGG	2 cut(s)	24, 185
BseDI	CCNNGG	6 cut(s)	162, 171, 222, 369, 445, 460
BseGI	GGATG	1 cut(s)	172
BseLI	CCNNNNNNNGG	6 cut(s)	29, 81, 149, 328, 465, 512
BseMI	GCAATG	1 cut(s)	294
BseMII	CTCAG	3 cut(s)	189, 428, 446
BseXI	GCAGC	1 cut(s)	45
Bsh1236I	CGCG	1 cut(s)	70
Bsh1285I	CGRYCG	1 cut(s)	108
BshTI	ACCGGT	1 cut(s)	93
BsiEI	CGRYCG	1 cut(s)	108
BsiHKCI	CYCGRG	1 cut(s)	376
BsiSI	CCGG	1 cut(s)	94
BslI	CCNNNNNNNGG	6 cut(s)	29, 81, 149, 328, 465, 512
BsmI	GAATGC	1 cut(s)	194
BsoBI	CYCGRG	1 cut(s)	376
Bsp1286I	GDGCHC	1 cut(s)	377
Bsp143I	GATC	2 cut(s)	105, 261
Bsp1720I	GCTNAGC	1 cut(s)	414
BspACI	CCGC	8 cut(s)	5, 27, 30, 45, 48, 60, 68, 387
BspCNI	CTCAG	3 cut(s)	190, 427, 445
BspFNI	CGCG	1 cut(s)	70
BspHI	TCATGA	1 cut(s)	126
BsrBI	CCGCTC	2 cut(s)	45, 60
BsrDI	GCAATG	1 cut(s)	294
BsrFI	RCCGGY	1 cut(s)	93
BssAI	RCCGGY	1 cut(s)	93
BssECI	CCNNGG	6 cut(s)	162, 171, 222, 369, 445, 460
BssMI	GATC	2 cut(s)	105, 261
BssSI	CACGAG	1 cut(s)	38
BssT1I	CCWWGG	5 cut(s)	162, 171, 369, 445, 460
Bst2BI	CACGAG	1 cut(s)	38
Bst2UI	CCWGG	2 cut(s)	24, 185
Bst4CI	ACNGT	2 cut(s)	181, 269
Bst6I	CTCTTC	1 cut(s)	245
BstC8I	GCNNGC	2 cut(s)	68, 442
BstDEI	CTNAG	3 cut(s)	198, 414, 432
BstENI	CCTNNNNNAGG	1 cut(s)	510
BstF5I	GGATG	1 cut(s)	172
BstFNI	CGCG	1 cut(s)	70
BstH2I	RGCGCY	1 cut(s)	140
BstHHI	GCGC	2 cut(s)	72, 139
BstKTI	GATC	2 cut(s)	108, 264
BstMBI	GATC	2 cut(s)	105, 261
BstMCI	CGRYCG	1 cut(s)	108
BstMWI	GCNNNNNNNGC	4 cut(s)	33, 42, 57, 167
BstNI	CCWGG	2 cut(s)	24, 185
BstSCI	CCNGG	2 cut(s)	22, 183
BstUI	CGCG	1 cut(s)	70
BstV1I	GCAGC	1 cut(s)	45
BstV2I	GAAGAC	1 cut(s)	263
BtsCI	GGATG	1 cut(s)	172
Cac8I	GCNNGC	2 cut(s)	68, 442
CciI	TCATGA	1 cut(s)	126
CfoI	GCGC	2 cut(s)	72, 139
Cfr10I	RCCGGY	1 cut(s)	93
Cfr13I	GGNCC	1 cut(s)	91
CspAI	ACCGGT	1 cut(s)	93
CviAII	CATG	1 cut(s)	127
CviJI	RGCY	9 cut(s)	11, 36, 66, 120, 210, 303, 375, 418, 503
CviKI_1	RGCY	9 cut(s)	11, 36, 66, 120, 210, 303, 375, 418, 503
DdeI	CTNAG	3 cut(s)	198, 414, 432
DpnI	GATC	2 cut(s)	107, 263
DpnII	GATC	2 cut(s)	105, 261
Eam1104I	CTCTTC	1 cut(s)	245
EarI	CTCTTC	1 cut(s)	245
EciI	GGCGGA	2 cut(s)	20, 402
Eco130I	CCWWGG	5 cut(s)	162, 171, 369, 445, 460
Eco24I	GRGCYC	1 cut(s)	377
Eco47I	GGWCC	1 cut(s)	91
Eco88I	CYCGRG	1 cut(s)	376
EcoNI	CCTNNNNNAGG	1 cut(s)	510
EcoRII	CCWGG	2 cut(s)	22, 183
EcoT14I	CCWWGG	5 cut(s)	162, 171, 369, 445, 460
EcoT38I	GRGCYC	1 cut(s)	377
ErhI	CCWWGG	5 cut(s)	162, 171, 369, 445, 460
FaeI	CATG	1 cut(s)	130
FaiI	YATR	3 cut(s)	128, 315, 509
FatI	CATG	1 cut(s)	126
FauI	CCCGC	1 cut(s)	75
Fnu4HI	GCNGC	5 cut(s)	28, 31, 34, 46, 49
FokI	GGATG	1 cut(s)	179
FriOI	GRGCYC	1 cut(s)	377
Fsp4HI	GCNGC	5 cut(s)	28, 31, 34, 46, 49
GlaI	GCGC	2 cut(s)	71, 138
GluI	GCNGC	5 cut(s)	28, 31, 34, 46, 49
HaeII	RGCGCY	1 cut(s)	140
HapII	CCGG	1 cut(s)	94
HhaI	GCGC	2 cut(s)	72, 139
Hin1II	CATG	1 cut(s)	130
Hin6I	GCGC	2 cut(s)	70, 137
HinP1I	GCGC	2 cut(s)	70, 137
HinfI	GANTC	2 cut(s)	19, 392
HpaII	CCGG	1 cut(s)	94
HphI	GGTGA	4 cut(s)	208, 281, 331, 374
Hpy166II	GTNNAC	1 cut(s)	334
Hpy188I	TCNGA	6 cut(s)	105, 147, 199, 223, 435, 520
Hpy188III	TCNNGA	2 cut(s)	83, 127
Hpy8I	GTNNAC	1 cut(s)	334
Hpy99I	CGWCG	1 cut(s)	56
HpyAV	CCTTC	2 cut(s)	127, 376
HpyCH4III	ACNGT	2 cut(s)	181, 269
HpyCH4IV	ACGT	1 cut(s)	78
HpyCH4V	TGCA	1 cut(s)	511
HpyF10VI	GCNNNNNNNGC	4 cut(s)	33, 42, 57, 167
HpyF3I	CTNAG	3 cut(s)	198, 414, 432
HpySE526I	ACGT	1 cut(s)	78
Hsp92II	CATG	1 cut(s)	130
HspAI	GCGC	2 cut(s)	70, 137
Kzo9I	GATC	2 cut(s)	105, 261
LmnI	GCTCC	5 cut(s)	16, 57, 77, 85, 508
LpnPI	CCDG	8 cut(s)	9, 36, 74, 107, 170, 197, 254, 268
Lsp1109I	GCAGC	1 cut(s)	45
LweI	GCATC	2 cut(s)	157, 400
MaeII	ACGT	1 cut(s)	78
MaeIII	GTNAC	1 cut(s)	230
MalI	GATC	2 cut(s)	107, 263
MbiI	CCGCTC	2 cut(s)	45, 60
MboI	GATC	2 cut(s)	105, 261
MboII	GAAGA	5 cut(s)	92, 142, 262, 268, 390
MhlI	GDGCHC	1 cut(s)	377
MluCI	AATT	2 cut(s)	469, 478
MlyI	GAGTC	1 cut(s)	28
MmeI	TCCRAC	1 cut(s)	99
MspI	CCGG	1 cut(s)	94
MspR9I	CCNGG	2 cut(s)	24, 185
Mva1269I	GAATGC	1 cut(s)	194
MvaI	CCWGG	2 cut(s)	24, 185
MvnI	CGCG	1 cut(s)	70
MwoI	GCNNNNNNNGC	4 cut(s)	33, 42, 57, 167
NdeII	GATC	2 cut(s)	105, 261
NlaIII	CATG	1 cut(s)	130
NmuCI	GTSAC	1 cut(s)	230
PagI	TCATGA	1 cut(s)	126
PctI	GAATGC	1 cut(s)	194
PfeI	GAWTC	1 cut(s)	392
PfoI	TCCNGGA	1 cut(s)	183
PinAI	ACCGGT	1 cut(s)	93
PkrI	GCNGC	5 cut(s)	29, 32, 35, 47, 50
Ple19I	CGATCG	1 cut(s)	108
PleI	GAGTC	1 cut(s)	27
PpsI	GAGTC	1 cut(s)	27
Psp1406I	AACGTT	1 cut(s)	78
Psp6I	CCWGG	2 cut(s)	22, 183
PspGI	CCWGG	2 cut(s)	22, 183
PspPI	GGNCC	1 cut(s)	91
PvuI	CGATCG	1 cut(s)	108
SatI	GCNGC	5 cut(s)	28, 31, 34, 46, 49
Sau3AI	GATC	2 cut(s)	105, 261
Sau96I	GGNCC	1 cut(s)	91
SchI	GAGTC	1 cut(s)	28
ScrFI	CCNGG	2 cut(s)	24, 185
SduI	GDGCHC	1 cut(s)	377
SetI	ASST	9 cut(s)	13, 81, 212, 221, 333, 339, 357, 451, 505
SfaNI	GCATC	2 cut(s)	157, 400
SinI	GGWCC	1 cut(s)	91
SmlI	CTYRAG	1 cut(s)	326
SmoI	CTYRAG	1 cut(s)	326
Sse9I	AATT	2 cut(s)	469, 478
SsiI	CCGC	8 cut(s)	5, 27, 30, 45, 48, 60, 68, 387
StyD4I	CCNGG	2 cut(s)	22, 183
StyI	CCWWGG	5 cut(s)	162, 171, 369, 445, 460
TaaI	ACNGT	2 cut(s)	181, 269
TaiI	ACGT	1 cut(s)	81
TaqI	TCGA	1 cut(s)	318
TasI	AATT	2 cut(s)	469, 478
TauI	GCSGC	4 cut(s)	30, 33, 48, 51
TfiI	GAWTC	1 cut(s)	392
TseFI	GTSAC	1 cut(s)	230
TseI	GCWGC	1 cut(s)	33
Tsp45I	GTSAC	1 cut(s)	230
TspDTI	ATGAA	4 cut(s)	143, 193, 202, 384
VpaK11BI	GGWCC	1 cut(s)	91
XagI	CCTNNNNNAGG	1 cut(s)	510
XapI	RAATTY	1 cut(s)	478

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.