Rh1AG429500

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001854
Physical Location & Seq
Forward (+)
65674013 .. 65676737
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG429500.1

Sequence Viewer

Length: 888 bp
ATGGTGTTTGTAAAGGCCAACGCTAGTGATTGGGTTGCTGATTGGATGATTAATCCAGGTCAGCGTGTTATGGGGATGATGGCGGCAAGGGCCGGCATCGGTGTTTGCTCAGACTGCGGCGAAGATCGCTGGCAGAATTGGGGTGATATTGGGTTTATGGTGCTCTGCTGGTCTTCGGAGCTAGTTTGCCCAGAATCGGATCCGCGTGCTACTAGTGGTGGTGGAAGAGCGGGTGTCGATGGGATTAACCCCGATTCATGTTCACAAACTCGTATTGAAGACTGTTACATAGTCTCGGGGGATGACTGCATTGCAGTGAAGAGTGGCTGGGATCAGGATGGAATCAACTTCGGCATGCCAACCGAGCATCTTGTTATCAGGAGGGTCACCTGCATTTCACCTAAAAGTGATACCGTTGCTCTAGGCAGTGAAATGTCCGGTGGAATCAGAGATGTTAGAGTTGAGGATGTCACATCCATTAACACTGAATCTAGTATTAGGATCAAAACAGCTAAAGAGAGAGGGGCTGATGTCAATGACATCTTTGTACAACGATTAACATTGAACACTGTTAGTATAGGAACTTGCCCATATGGTTCGCATCCAGACGAGGGATTTGATCCAAAGGCACTTCCTGTGATTCTAAACATCAACTACAGAGAGATTGTCGCTGAGAATGTTACCAGGCCAGCACAGTTGGATGGACTTGCTAACGACCCTTTCAAGGGAGTTTGCATTTCTAATGTGAGTATCTCATTGACTGCAAAGCTTAAGGAGCTGTTGTGGAATTGTACTGATGTTGAGGGCATTAATTACAAGCAATGTGACTCCAACGACTTGCGATTTGTTGCCCGAGCAGCAAAACCCAGTAGGTTGTGCTTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

295

Amino Acids

32.09

Weight (kDa)

4.8

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 78 - 267 1.9e-14 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 398
Acc36I ACCTGC 1 cut(s) 398
AccBSI CCGCTC 1 cut(s) 230
AccII CGCG 1 cut(s) 205
AciI CCGC 4 cut(s) 83, 117, 203, 230
AclWI GGATC 5 cut(s) 194, 207, 339, 509, 614
AfaI GTAC 2 cut(s) 549, 793
AfiI CCNNNNNNNGG 4 cut(s) 196, 611, 724, 725
AflII CTTAAG 1 cut(s) 770
AgsI TTSAA 3 cut(s) 278, 565, 724
AhlI ACTAGT 1 cut(s) 212
AjnI CCWGG 2 cut(s) 55, 683
AluBI AGCT 4 cut(s) 181, 512, 769, 778
AluI AGCT 4 cut(s) 181, 512, 769, 778
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 298
AlwI GGATC 5 cut(s) 194, 207, 339, 509, 614
Ama87I CYCGRG 2 cut(s) 295, 852
AoxI GGCC 3 cut(s) 15, 90, 686
ApeKI GCWGC 1 cut(s) 857
ArsI GACNNNNNNTTYG 4 cut(s) 599, 631, 827, 859
AseI ATTAAT 2 cut(s) 51, 810
AspS9I GGNCC 1 cut(s) 90
AsuHPI GGTGA 3 cut(s) 155, 379, 390
AvaI CYCGRG 2 cut(s) 295, 852
BamHI GGATCC 1 cut(s) 199
BbsI GAAGAC 2 cut(s) 165, 285
Bbv12I GWGCWC 1 cut(s) 165
BbvI GCAGC 1 cut(s) 869
BccI CCATC 4 cut(s) 73, 233, 332, 695
BciT130I CCWGG 2 cut(s) 57, 685
BcoDI GTCTC 1 cut(s) 298
BcuI ACTAGT 1 cut(s) 212
BfaI CTAG 5 cut(s) 24, 182, 213, 422, 492
BfmI CTRYAG 1 cut(s) 655
BfrI CTTAAG 1 cut(s) 770
BfuAI ACCTGC 1 cut(s) 398
BisI GCNGC 3 cut(s) 84, 118, 858
BlsI GCNGC 3 cut(s) 85, 119, 859
Bme1390I CCNGG 2 cut(s) 57, 685
BmeT110I CYCGRG 2 cut(s) 295, 852
BmgT120I GGNCC 1 cut(s) 90
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 2 cut(s) 57, 685
BmrI ACTGGG 1 cut(s) 861
BmsI GCATC 3 cut(s) 105, 376, 610
BmuI ACTGGG 1 cut(s) 861
BpiI GAAGAC 2 cut(s) 165, 285
BsaWI WCCGGW 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 767, 797
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 611, 724, 725
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 1 cut(s) 867
Bse3DI GCAATG 2 cut(s) 309, 827
BseBI CCWGG 2 cut(s) 57, 685
BseGI GGATG 8 cut(s) 51, 81, 307, 343, 472, 473, 601, 706
BseLI CCNNNNNNNGG 4 cut(s) 196, 611, 724, 725
BseMI GCAATG 2 cut(s) 309, 827
BseMII CTCAG 2 cut(s) 123, 663
BseNI ACTGG 1 cut(s) 867
BseXI GCAGC 1 cut(s) 869
BseYI CCCAGC 1 cut(s) 327
Bsh1236I CGCG 1 cut(s) 205
BshFI GGCC 3 cut(s) 17, 92, 688
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 2 cut(s) 295, 852
BsiSI CCGG 2 cut(s) 93, 438
BslI CCNNNNNNNGG 4 cut(s) 196, 611, 724, 725
BsmAI GTCTC 1 cut(s) 298
BsnI GGCC 3 cut(s) 17, 92, 688
BsoBI CYCGRG 2 cut(s) 295, 852
Bsp1286I GDGCHC 1 cut(s) 165
Bsp1407I TGTACA 1 cut(s) 547
Bsp143I GATC 5 cut(s) 124, 199, 331, 501, 619
BspACI CCGC 4 cut(s) 83, 117, 203, 230
BspANI GGCC 3 cut(s) 17, 92, 688
BspCNI CTCAG 2 cut(s) 122, 664
BspFNI CGCG 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 201
BspMI ACCTGC 1 cut(s) 398
BspPI GGATC 5 cut(s) 194, 207, 339, 509, 614
BspQI GCTCTTC 1 cut(s) 220
BspTI CTTAAG 1 cut(s) 770
BsrBI CCGCTC 1 cut(s) 230
BsrDI GCAATG 2 cut(s) 309, 827
BsrFI RCCGGY 1 cut(s) 92
BsrGI TGTACA 1 cut(s) 547
BsrI ACTGG 1 cut(s) 867
BssAI RCCGGY 1 cut(s) 92
BssMI GATC 5 cut(s) 124, 199, 331, 501, 619
Bst2UI CCWGG 2 cut(s) 57, 685
Bst4CI ACNGT 4 cut(s) 284, 415, 571, 696
Bst6I CTCTTC 2 cut(s) 220, 314
BstAFI CTTAAG 1 cut(s) 770
BstAUI TGTACA 1 cut(s) 547
BstC8I GCNNGC 5 cut(s) 94, 131, 207, 356, 690
BstDEI CTNAG 2 cut(s) 109, 672
BstEII GGTNACC 1 cut(s) 385
BstF5I GGATG 8 cut(s) 51, 81, 307, 343, 472, 473, 601, 706
BstFNI CGCG 1 cut(s) 205
BstKTI GATC 5 cut(s) 127, 202, 334, 504, 622
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 5 cut(s) 124, 199, 331, 501, 619
BstMWI GCNNNNNNNGC 6 cut(s) 89, 114, 126, 364, 775, 857
BstNI CCWGG 2 cut(s) 57, 685
BstNSI RCATGY 1 cut(s) 358
BstPI GGTNACC 1 cut(s) 385
BstSCI CCNGG 2 cut(s) 55, 683
BstSFI CTRYAG 1 cut(s) 655
BstUI CGCG 1 cut(s) 205
BstV1I GCAGC 1 cut(s) 869
BstV2I GAAGAC 2 cut(s) 165, 285
BstX2I RGATCY 1 cut(s) 199
BstYI RGATCY 1 cut(s) 199
BsuRI GGCC 3 cut(s) 17, 92, 688
BtsCI GGATG 8 cut(s) 51, 81, 307, 343, 472, 473, 601, 706
BtsI GCAGTG 2 cut(s) 321, 433
BtsIMutI CAGTG 4 cut(s) 321, 433, 483, 567
BveI ACCTGC 1 cut(s) 398
Cac8I GCNNGC 5 cut(s) 94, 131, 207, 356, 690
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 90
Csp6I GTAC 2 cut(s) 548, 792
CviAII CATG 2 cut(s) 258, 355
CviJI RGCY 9 cut(s) 17, 92, 181, 327, 512, 527, 688, 769, 778
CviKI_1 RGCY 9 cut(s) 17, 92, 181, 327, 512, 527, 688, 769, 778
CviQI GTAC 2 cut(s) 548, 792
DdeI CTNAG 2 cut(s) 109, 672
DpnI GATC 5 cut(s) 126, 201, 333, 503, 621
DpnII GATC 5 cut(s) 124, 199, 331, 501, 619
Eam1104I CTCTTC 2 cut(s) 220, 314
EarI CTCTTC 2 cut(s) 220, 314
Eco88I CYCGRG 2 cut(s) 295, 852
Eco91I GGTNACC 1 cut(s) 385
EcoO65I GGTNACC 1 cut(s) 385
EcoRII CCWGG 2 cut(s) 55, 683
FaeI CATG 2 cut(s) 261, 358
FaiI YATR 8 cut(s) 71, 158, 259, 290, 356, 578, 592, 594
FatI CATG 2 cut(s) 257, 354
FauI CCCGC 1 cut(s) 223
FauNDI CATATG 1 cut(s) 592
Fnu4HI GCNGC 3 cut(s) 84, 118, 858
FokI GGATG 8 cut(s) 58, 88, 314, 350, 460, 479, 588, 713
Fsp4HI GCNGC 3 cut(s) 84, 118, 858
FspBI CTAG 5 cut(s) 24, 182, 213, 422, 492
GluI GCNGC 3 cut(s) 84, 118, 858
GsaI CCCAGC 1 cut(s) 331
HaeIII GGCC 3 cut(s) 17, 92, 688
HapII CCGG 2 cut(s) 93, 438
Hin1II CATG 2 cut(s) 261, 358
HindIII AAGCTT 1 cut(s) 767
HinfI GANTC 7 cut(s) 194, 254, 342, 444, 488, 640, 827
HpaII CCGG 2 cut(s) 93, 438
HphI GGTGA 3 cut(s) 155, 379, 390
Hpy166II GTNNAC 1 cut(s) 263
Hpy188I TCNGA 4 cut(s) 112, 178, 199, 449
Hpy188III TCNNGA 3 cut(s) 335, 379, 605
Hpy8I GTNNAC 1 cut(s) 263
HpyCH4III ACNGT 4 cut(s) 284, 415, 571, 696
HpyCH4V TGCA 5 cut(s) 309, 314, 393, 735, 764
HpyF10VI GCNNNNNNNGC 6 cut(s) 89, 114, 126, 364, 775, 857
HpyF3I CTNAG 2 cut(s) 109, 672
Hsp92II CATG 2 cut(s) 261, 358
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 5 cut(s) 124, 199, 331, 501, 619
LguI GCTCTTC 1 cut(s) 220
LmnI GCTCC 2 cut(s) 178, 775
Lsp1109I GCAGC 1 cut(s) 869
LweI GCATC 3 cut(s) 105, 376, 610
MaeI CTAG 5 cut(s) 24, 182, 213, 422, 492
MaeIII GTNAC 5 cut(s) 284, 385, 469, 679, 824
MalI GATC 5 cut(s) 126, 201, 333, 503, 621
MbiI CCGCTC 1 cut(s) 230
MboI GATC 5 cut(s) 124, 199, 331, 501, 619
MboII GAAGA 5 cut(s) 134, 165, 237, 290, 331
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 3 cut(s) 136, 787, 811
MlyI GAGTC 1 cut(s) 821
MmeI TCCRAC 2 cut(s) 678, 855
MnlI CCTC 5 cut(s) 375, 457, 515, 604, 796
MroNI GCCGGC 1 cut(s) 92
MseI TTAA 6 cut(s) 51, 246, 480, 557, 771, 810
MslI CAYNNNNRTG 1 cut(s) 314
MspCI CTTAAG 1 cut(s) 770
MspI CCGG 2 cut(s) 93, 438
MspR9I CCNGG 2 cut(s) 57, 685
MvaI CCWGG 2 cut(s) 57, 685
MvnI CGCG 1 cut(s) 205
MwoI GCNNNNNNNGC 6 cut(s) 89, 114, 126, 364, 775, 857
NaeI GCCGGC 1 cut(s) 94
NdeI CATATG 1 cut(s) 592
NdeII GATC 5 cut(s) 124, 199, 331, 501, 619
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 2 cut(s) 261, 358
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 3 cut(s) 385, 469, 824
NspI RCATGY 1 cut(s) 358
PaeI GCATGC 1 cut(s) 358
PaqCI CACCTGC 1 cut(s) 398
PciSI GCTCTTC 1 cut(s) 220
PdiI GCCGGC 1 cut(s) 94
PfeI GAWTC 6 cut(s) 194, 254, 342, 444, 488, 640
PkrI GCNGC 3 cut(s) 85, 119, 859
PleI GAGTC 1 cut(s) 821
PpsI GAGTC 1 cut(s) 821
PshBI ATTAAT 2 cut(s) 51, 810
Psp6I CCWGG 2 cut(s) 55, 683
PspEI GGTNACC 1 cut(s) 385
PspFI CCCAGC 1 cut(s) 327
PspGI CCWGG 2 cut(s) 55, 683
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 1 cut(s) 90
PsuI RGATCY 1 cut(s) 199
RsaI GTAC 2 cut(s) 549, 793
RsaNI GTAC 2 cut(s) 548, 792
RseI CAYNNNNRTG 1 cut(s) 314
SapI GCTCTTC 1 cut(s) 220
SaqAI TTAA 6 cut(s) 51, 246, 480, 557, 771, 810
SatI GCNGC 3 cut(s) 84, 118, 858
Sau3AI GATC 5 cut(s) 124, 199, 331, 501, 619
Sau96I GGNCC 1 cut(s) 90
SchI GAGTC 1 cut(s) 821
ScrFI CCNGG 2 cut(s) 57, 685
SduI GDGCHC 1 cut(s) 165
SetI ASST 8 cut(s) 61, 183, 392, 403, 514, 771, 780, 875
SfaNI GCATC 3 cut(s) 105, 376, 610
SfcI CTRYAG 1 cut(s) 655
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
SpeI ACTAGT 1 cut(s) 212
SphI GCATGC 1 cut(s) 358
Sse9I AATT 3 cut(s) 136, 787, 811
SsiI CCGC 4 cut(s) 83, 117, 203, 230
SspMI CTAG 5 cut(s) 24, 182, 213, 422, 492
StyD4I CCNGG 2 cut(s) 55, 683
TaaI ACNGT 4 cut(s) 284, 415, 571, 696
TaqI TCGA 1 cut(s) 237
TasI AATT 3 cut(s) 136, 787, 811
TatI WGTACW 2 cut(s) 547, 791
TauI GCSGC 2 cut(s) 86, 120
TfiI GAWTC 6 cut(s) 194, 254, 342, 444, 488, 640
Tru1I TTAA 6 cut(s) 51, 246, 480, 557, 771, 810
Tru9I TTAA 6 cut(s) 51, 246, 480, 557, 771, 810
TscAI CASTG 4 cut(s) 321, 433, 490, 574
TseFI GTSAC 3 cut(s) 385, 469, 824
TseI GCWGC 1 cut(s) 857
Tsp45I GTSAC 3 cut(s) 385, 469, 824
TspDTI ATGAA 1 cut(s) 246
TspRI CASTG 4 cut(s) 321, 433, 490, 574
Vha464I CTTAAG 1 cut(s) 770
VspI ATTAAT 2 cut(s) 51, 810
XceI RCATGY 1 cut(s) 358
XspI CTAG 5 cut(s) 24, 182, 213, 422, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.